公开(公告)号：US5190872A

公开(公告)日：1993-03-02

申请号：US573066

申请日：1990-08-27

申请人： Yoshio Hashizume ,  Akio Kariyone ,  Ryuzo Hayashi ,  Minako Oka

发明人： Yoshio Hashizume ,  Akio Kariyone ,  Ryuzo Hayashi ,  Minako Oka

IPC分类号： G01N27/327 ,  C12N11/00 ,  C12N11/06 ,  C12N11/14 ,  C12Q1/00 ,  G01N27/416

CPC分类号： C12N11/06 ,  C12Q1/003 ,  Y10S435/817

摘要： Alcohol oxidase is immobilized by bonding an aminosilane coupling agent to a carrier, bonding a multifunctional aldehyde such as glutaraldehyde to an amino group of the aminosilane, washing the carrier to remove free multifunctional aldehyde and bonding alcohol oxidase to the multifunctional aldehyde bonded to the aminosilane. Washing to remove free multifunctional aldehyde enables immobilizing a one molecule thickness of alcohol oxidase on the carrier since free multifunctional aldehyde is not present to cross-link alcohol oxidase molecules together. A thin layer of alcohol oxidase is advantageous when assaying for alcohol since a thin layer does not retain hydrogen peroxide that can deactivate alcohol oxidase. The carrier preferably contains hydroxyl groups and is porous, and can be a silicate-containing carrier such as diatomaceous earth or fire brick. The immobilized alcohol oxidase is used in a column as part of an apparatus for measuring alcohol or as part of an apparatus containing immobilized glucose oxidase for measuring glucose and alcohol.

公开(公告)号：US5320939A

公开(公告)日：1994-06-14

申请号：US953294

申请日：1992-09-30

申请人： Yoshio Hashizume ,  Ryuzo Hayashi ,  Akio Kariyone

发明人： Yoshio Hashizume ,  Ryuzo Hayashi ,  Akio Kariyone

IPC分类号： G01N27/26 ,  C12Q1/00 ,  G01N27/27 ,  G01N27/403 ,  G01N27/416

CPC分类号： C12Q1/001 ,  Y10S435/817

摘要： In a two-substrate measuring apparatus employing a first enzyme electrode for responding only to a first substrate to be measured and a second enzyme electrode for responding to both first and second substrates to be measured, in which the same enzymatic reactions are used for forming detectable substances and the substances to be finally detected by the electrodes are identical, when calculating the calibration curve for the second substrate to be measured, the output portion due to traces of the first substrate contained in the second substrate to be measured is removed, so that the second substrate is measured at a high sensitivity and high accuracy.

摘要翻译： 在使用第一酶电极仅响应待测量的第一基底的第二酶电极和用于响应于待测量的第一和第二基底的第二酶电极的双基底测量装置中，其中使用相同的酶反应形成可检测的 物质和由电极最终检测到的物质在计算待测量的第二基板的校准曲线时是相同的，除去由于包含在待测量的第二基板中的第一基板的痕迹引起的输出部分，从而 以高灵敏度和高精度测量第二基板。

公开(公告)号：US5992221A

公开(公告)日：1999-11-30

申请号：US703700

申请日：1996-08-27

申请人： Akio Kariyone ,  Yoshio Hashizume ,  Ryuzo Hayashi

发明人： Akio Kariyone ,  Yoshio Hashizume ,  Ryuzo Hayashi

IPC分类号： G01N1/00 ,  G01N1/38 ,  G01N1/16 ,  G01N15/06

CPC分类号： G01N1/38 ,  G01N35/1095

摘要： Pipes provided with each switching valve in intermediate portions thereof for feeding sample solutions to a separation mechanism are joined by a multi-way joint, to reach the separation mechanism through a pump. When all valves are closed while driving the pump and thereafter one valve is opened, all bubbles etc. downstream the opened valve are swept and eliminated due to abrupt flow of a liquid since a portion downstream the valve has been decompressed. In switching of a sample in sample measurement, a valve of a pipe for a completely measured sample is left open while a valve of a pipe for a next sample is opened, and then the valve of the pipe for the previously completely measured sample is closed after a while. Thus, pressure fluctuation following sample switching is avoided.

摘要翻译： 在其中间部分设置有用于将样品溶液供给到分离机构的每个切换阀的管道通过多向接头连接，以通过泵到达分离机构。 当所有阀在驱动泵时关闭，此后一个阀打开，所以打开的阀下游的所有气泡等均由于液体的急剧流动被扫除并消除，因为阀下游的部分已被减压。 在样品测量中切换样品时，用于完全测量样品的管道的阀门打开，而用于下一个样品的管道的阀门打开，然后用于先前完全测量的样品的管道阀门关闭 过了一会儿。 因此，避免了样品切换后的压力波动。

公开(公告)号：US5225321A

公开(公告)日：1993-07-06

申请号：US727341

申请日：1991-07-08

申请人： Ryuzo Hayashi ,  Akio Kariyone ,  Yoshio Hashizume

发明人： Ryuzo Hayashi ,  Akio Kariyone ,  Yoshio Hashizume

IPC分类号： C12Q1/00 ,  G01N27/403

CPC分类号： C12Q1/001 ,  C12Q1/005 ,  C12Q1/006

摘要： In the case of measuring concentration of, for example, glucose as a first substance to be measured, and that of sucrose as a second substance to be measured, using a measuring apparatus, the apparatus is provided with a first enzyme electrode, a dilution pipeline situated at the downstream of the electrode and for diluting the substances, and a second enzyme electrode situated at the downstream of the dilution pipeline. A sample, which contains the first and second substances, is poured intermittently into a constant flow of a buffer solution. The first enzyme electrode detects the first substance. The second enzyme electrode, which comprises an immobilized enzyme catalizing the conversion of the second substance in to the first substance, detects the first substance, which is originally contained in the sample as well as is formed on the second enzyme electrode. The concentration of the second substance in the sample can be determined by calculating about the outputs of the first and second enzyme electrodes.

摘要翻译： 在测量作为第一待测物质的葡萄糖的浓度和作为第二待测物质的蔗糖的浓度的情况下，使用测量装置，设置有第一酶电极，稀释管线 位于电极的下游并用于稀释物质，以及位于稀释管道下游的第二酶电极。 将含有第一和第二物质的样品间歇地倒入缓冲溶液的恒定流中。 第一酶电极检测第一物质。 第二酶电极，其包含催化第二物质向第一物质转化的固定化酶，检测原样包含在样品中的第一物质，并形成在第二酶电极上。 可以通过计算第一和第二酶电极的输出来确定样品中第二物质的浓度。

公开(公告)号：US5221521A

公开(公告)日：1993-06-22

申请号：US735911

申请日：1991-07-25

申请人： Yoshio Hashizume ,  Akio Kariyone ,  Ryuzo Hayashi

发明人： Yoshio Hashizume ,  Akio Kariyone ,  Ryuzo Hayashi

IPC分类号： G01N1/38 ,  G01N35/08

CPC分类号： G01N1/38 ,  G01N35/085 ,  Y10T436/117497 ,  Y10T436/118339 ,  Y10T436/25 ,  Y10T436/25625 ,  Y10T436/2575

摘要： A liquid dilution system which dilutes a sample to be analyzed with a carrier to supply a diluted sample-containing liquid to an analytical measurement apparatus of flow type. The inventive dilution system features simplicity and versatility as compared to conventional automatic apparatus with robot concept, and the present system comprises: a first carrier pump for feeding a first carrier; a sample injection unit for injecting the sample into the first carrier; a main passage for flowing a liquid from the sample injection unit to a detector unit of an analytical measurement apparatus; a branching device located downstream of the sample injection unit for forming a branched passage to remove a liquid mass partially from the main passage; a confluence unit located downstream of the branching unit for confluencing a second carrier; and a second carrier pump for feeding the second carrier to the confluence unit. Dilution and mixing of the sample with a carrier is done twice, once at the sample injection and then again at confluence.

公开(公告)号：US5081015A

公开(公告)日：1992-01-14

申请号：US401132

申请日：1989-08-30

申请人： Ryuzo Hayashi ,  Akio Kariyone ,  Yoshio Hashizume

发明人： Ryuzo Hayashi ,  Akio Kariyone ,  Yoshio Hashizume

IPC分类号： C12Q1/00

CPC分类号： C12Q1/005 ,  Y10S435/817 ,  Y10S435/921 ,  Y10S435/938

摘要： An enzyme electrode possessing an immobilized enzyme membrane or an immobilized enzyme layer composed by applying a solution containing alcohol oxidase and a crosslinking agent, in which the solution further contains reduced glutathione.According to the invention it is possible to immobilize alcohol oxidase stably, and an excellent immobilized enzyme electrode for alcohol measurement is obtained. Furthermore, by this invention, a highly sensitive and stable measuring method is conducted quite easily.

公开(公告)号：US5242793A

公开(公告)日：1993-09-07

申请号：US490709

申请日：1990-03-07

申请人： Akio Kariyone ,  Ryuzo Hayashi ,  Yoshio Hashizume

发明人： Akio Kariyone ,  Ryuzo Hayashi ,  Yoshio Hashizume

IPC分类号： B01D69/14 ,  C12N11/02 ,  C12N11/10 ,  C12Q1/00 ,  G01N27/40

CPC分类号： C12Q1/003 ,  B01D69/141 ,  C12N11/02 ,  C12N11/10 ,  C12Q1/002 ,  G01N27/3271 ,  Y10S435/817

摘要： An electrode comprising an electrically conductive base, and a selectively permeable membrane which is produced by forming a membrane from a mixed solution comprising (a) albumin, (b) at least one type of crosslinking agent and (c) chitosan. The selectively permeable membrane is useful, e.g., in conjuction with an electrode. An enzyme electrode is also provided having the selectively permeable membrane disposed on the electrode and an immobilized enzyme membrane disposed on the selectively permeable membrane. The immobilized enzyme membrane is prepared by incorporating a carboxylate into the solution containing the enzyme and an aldehyde as the crosslinking agent. The crosslinking is effected by heating or drying.

公开(公告)号：US5081037A

公开(公告)日：1992-01-14

申请号：US321165

申请日：1989-03-09

申请人： Akio Kariyone ,  Yoshio Hashizume ,  Ryuzo Hayashi

发明人： Akio Kariyone ,  Yoshio Hashizume ,  Ryuzo Hayashi

IPC分类号： G01N27/327 ,  C12M1/40 ,  C12Q1/00 ,  G01N27/30 ,  G01N27/416

CPC分类号： C12Q1/005 ,  Y10S435/817 ,  Y10S435/913 ,  Y10S435/939

摘要： An enzyme electrode for measuring malto-oligosaccharides having a co-immobilized enzyme membrane containing glucoamylase and a glucose oxidizing enzyme such as glucose oxidase or pyranose oxidase.The glucoamylase and glucose oxidizing enzyme are immobilized at a ratio of ##EQU1## where Va is the maximum reaction rate of glucoamylase as expressed in the glucose formation rate from maltose, and Vo is the maximum reaction rate of glucose oxidizing enzyme as expressed in the hydrogen peroxide formation rate from glucose.In such enzyme electrode, malto-oligosaccharides are detected at the response value proportional to their degree of polymerization. Therefore, the malto-oligosaccharides can be measured at the glucose converted concentration.

公开(公告)号：US5306413A

公开(公告)日：1994-04-26

申请号：US37421

申请日：1993-03-26

申请人： Ryuzo Hayashi ,  Yukie Inoue ,  Akio Kariyone

发明人： Ryuzo Hayashi ,  Yukie Inoue ,  Akio Kariyone

IPC分类号： C12Q1/00 ,  G01N27/26

CPC分类号： C12Q1/005 ,  Y10S435/817

摘要： The present invention relates to a simple and yet multifunctional system for assay employing immobilized enzymes.

摘要翻译： 本发明涉及使用固定化酶的简单而又多功能的测定系统。

公开(公告)号：US4971901A

公开(公告)日：1990-11-20

申请号：US146202

申请日：1988-01-20

申请人： Ryuzo Hayashi ,  Akio Kariyone

发明人： Ryuzo Hayashi ,  Akio Kariyone

IPC分类号： G01N27/327 ,  C12Q1/00

CPC分类号： C12Q1/005 ,  Y10S435/817

摘要： An enzyme electrode is prepared by a process wherein an albumin solution containing 0.05 to 0.5% by weight of a multifunctional aldehyde is applied onto the surface of an electroconductive substrate while maintaining the atmospheric temperature at 0.degree. to 20.degree. C. to thereby form a crosslinked albumin layer on the surface of the electroconductive substrate. Thereafter a solution containing 0.5% by weight or less of a multifunctional aldehyde and a predetermined amount of an oxidase capable of producing hydrogen peroxide (H.sub.2 O.sub.2) is applied on the surface of the previously formed crosslinked albumin layer to form a H.sub.2 O.sub.2 productive oxidase containing layer.