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公开(公告)号：US20050277138A1
公开(公告)日：2005-12-15
申请号：US11114288
申请日：2005-04-26
CPC分类号： C07H21/02
摘要： The present invention relates to compositions and methods for the preparation of modified nucleic acids. In particular, the present invention provides novel reagents and chemistries for the generation of linkers, modified phosphoramidites, and modified solid supports.
摘要翻译： 本发明涉及用于制备修饰的核酸的组合物和方法。 特别地，本发明提供用于产生接头，改性亚磷酰胺和改性固体载体的新型试剂和化学物质。
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公开(公告)号：US07582436B2
公开(公告)日：2009-09-01
申请号：US11787843
申请日：2007-04-18
申请人： Jeff G. Hall , Zbigniev Skrzypczynski , Sarah Wayland , Ned D. Reimer , Luis P. Reynaldo , Joerg Baier , Victor Lyamichev , Bruce P. Neri
发明人： Jeff G. Hall , Zbigniev Skrzypczynski , Sarah Wayland , Ned D. Reimer , Luis P. Reynaldo , Joerg Baier , Victor Lyamichev , Bruce P. Neri
CPC分类号： C12Q1/6823 , C12Q1/6806 , C12Q1/6853 , C12Q2561/109 , C12Q2525/197
摘要： The present invention provides compositions comprising oligonucleotides that have 3′ end groups (e.g. lipophilic moieties) that are useful in invasive cleavage reactions such as the INVADER assay. Specifically, the present invention provides compositions containing oligonucleotides with 3′ end groups configured for generating a detectable signal in invasive cleavages assays with a high signal-to-background ratio, as well as methods for generating such compositions.
摘要翻译： 本发明提供了包含具有3'端基（例如亲脂部分）的寡核苷酸的组合物，其可用于浸润性切割反应，例如INVADER测定。 具体而言，本发明提供含有3'末端基团的寡核苷酸的组合物，其配置用于在具有高信号与背景比的侵入性切割分析中产生可检测信号，以及产生这种组合物的方法。
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公开(公告)号：US20050130179A1
公开(公告)日：2005-06-16
申请号：US10875094
申请日：2004-06-23
申请人： Victor Lyamichev , Zbigniev Skrzypczynski , Hatim Allawi , Sarah Wayland , Tsetska Takova , Bruce Neri , James Prudent , Jeff Hall
发明人： Victor Lyamichev , Zbigniev Skrzypczynski , Hatim Allawi , Sarah Wayland , Tsetska Takova , Bruce Neri , James Prudent , Jeff Hall
IPC分类号： G01N33/50 , C07F9/24 , C07F9/59 , C12N9/22 , C12N15/09 , C12Q1/68 , G01N27/447 , G01N33/58 , C07H21/04
CPC分类号： C12N9/22 , C07F9/2408 , C07F9/59 , C12Q1/6816 , C12Q1/6823 , C12Q1/683 , C12Q2525/197 , C12Q2565/125 , C12Q2563/107
摘要： The present invention relates to novel phosphoramidites, including positive and neutrally charged compounds. The present invention also provides charge tags for attachment to materials including solid supports and nucleic acids, wherein the charge tags increase or decrease the net charge of the material. The present invention further provides methods for separating and characterizing molecules based on the charge differentials between modified and unmodified materials.
摘要翻译： 本发明涉及新型亚磷酰胺，包括正中和中性化合物。 本发明还提供用于附着到包括固体支持物和核酸的材料的电荷标签，其中电荷标签增加或减少材料的净电荷。 本发明还提供了基于修饰材料和未改性材料之间的电荷差异分离和表征分子的方法。
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公开(公告)号：US20080293930A1
公开(公告)日：2008-11-27
申请号：US11787843
申请日：2007-04-18
申请人： Jeff G. Hall , Zbigniev Skrzypczynski , Sarah Wayland , Ned D. Reimer , Luis P. Reynaldo , Joerg Baier , Victor Lyamichev , Bruce P. Neri
发明人： Jeff G. Hall , Zbigniev Skrzypczynski , Sarah Wayland , Ned D. Reimer , Luis P. Reynaldo , Joerg Baier , Victor Lyamichev , Bruce P. Neri
IPC分类号： C07H1/00
CPC分类号： C12Q1/6823 , C12Q1/6806 , C12Q1/6853 , C12Q2561/109 , C12Q2525/197
摘要： The present invention provides compositions comprising oligonucleotides that have 3′ end groups (e.g. lipophilic moieties) that are useful in invasive cleavage reactions such as the INVADER assay. Specifically, the present invention provides compositions containing oligonucleotides with 3′ end groups configured for generating a detectable signal in invasive cleavages assays with a high signal-to-background ratio, as well as methods for generating such compositions.
摘要翻译： 本发明提供了包含具有3'端基（例如亲脂部分）的寡核苷酸的组合物，其可用于浸润性切割反应，例如INVADER测定。 具体而言，本发明提供含有3'末端基团的寡核苷酸的组合物，其配置用于在具有高信号与背景比的侵入性切割分析中产生可检测信号，以及产生这种组合物的方法。
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公开(公告)号：US20080071074A1
公开(公告)日：2008-03-20
申请号：US11805038
申请日：2007-05-22
IPC分类号： C07H21/00
CPC分类号： C07H21/00 , A61K49/0039 , A61K49/0054
摘要： The present invention relates to compositions useful as probes and in other applications and methods of their use. In some embodiments, nucleotides are prepared and functionalized with dyes. In some embodiments a first molecule is functionalized with an alkynyl group, a second molecule is functionalized with an azide group, and said first and second molecules are mixed under conditions to form a conjugate with a 1,2,3-triazol group. In further embodiments, a nucleotide is functionalized with an alkynyl group, a dye is functionalized with an azide group, and mixing the nucleotide and the dye forms a conjugate capable of emitting light.
摘要翻译： 本发明涉及可用作探针的组合物及其应用和使用方法。 在一些实施方案中，制备核苷酸并用染料官能化。 在一些实施方案中，第一分子用炔基官能化，第二分子用叠氮基官能化，并且所述第一和第二分子在条件下混合以形成具有1,2,3-三唑基团的缀合物。 在另外的实施方案中，核苷酸用炔基官能化，染料用叠氮化物官能化，并且混合核苷酸和染料形成能发光的共轭体。
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