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公开(公告)号：US20050130179A1
公开(公告)日：2005-06-16
申请号：US10875094
申请日：2004-06-23
申请人： Victor Lyamichev , Zbigniev Skrzypczynski , Hatim Allawi , Sarah Wayland , Tsetska Takova , Bruce Neri , James Prudent , Jeff Hall
发明人： Victor Lyamichev , Zbigniev Skrzypczynski , Hatim Allawi , Sarah Wayland , Tsetska Takova , Bruce Neri , James Prudent , Jeff Hall
IPC分类号： G01N33/50 , C07F9/24 , C07F9/59 , C12N9/22 , C12N15/09 , C12Q1/68 , G01N27/447 , G01N33/58 , C07H21/04
CPC分类号： C12N9/22 , C07F9/2408 , C07F9/59 , C12Q1/6816 , C12Q1/6823 , C12Q1/683 , C12Q2525/197 , C12Q2565/125 , C12Q2563/107
摘要： The present invention relates to novel phosphoramidites, including positive and neutrally charged compounds. The present invention also provides charge tags for attachment to materials including solid supports and nucleic acids, wherein the charge tags increase or decrease the net charge of the material. The present invention further provides methods for separating and characterizing molecules based on the charge differentials between modified and unmodified materials.
摘要翻译： 本发明涉及新型亚磷酰胺，包括正中和中性化合物。 本发明还提供用于附着到包括固体支持物和核酸的材料的电荷标签，其中电荷标签增加或减少材料的净电荷。 本发明还提供了基于修饰材料和未改性材料之间的电荷差异分离和表征分子的方法。
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公开(公告)号：US20110104682A1
公开(公告)日：2011-05-05
申请号：US12898490
申请日：2010-10-05
申请人： Hatim Allawi , Brad T. Argue , Christian Tor Bartholomay , LuAnne Chehak , Michelle L. Curtis , Peggy S. Eis , Jeff G. Hall , Hon S. Ip , Lin Ji , Michael Kaiser , Robert W. Kwiatkowski, JR. , Andrew A. Lukowiak , Victor Lyamichev , Natalie E. Lyamicheva , WuPo Ma , Bruce P. Neri , Sarah M. Olson , Marilyn C. Olson-Munoz , James J. Schaefer , Zbigniev Skrzypczynski , Tsetska Y. Takova , Lisa C. Thompson , Kevin L. Vedvik
发明人： Hatim Allawi , Brad T. Argue , Christian Tor Bartholomay , LuAnne Chehak , Michelle L. Curtis , Peggy S. Eis , Jeff G. Hall , Hon S. Ip , Lin Ji , Michael Kaiser , Robert W. Kwiatkowski, JR. , Andrew A. Lukowiak , Victor Lyamichev , Natalie E. Lyamicheva , WuPo Ma , Bruce P. Neri , Sarah M. Olson , Marilyn C. Olson-Munoz , James J. Schaefer , Zbigniev Skrzypczynski , Tsetska Y. Takova , Lisa C. Thompson , Kevin L. Vedvik
CPC分类号： C12Q1/6823 , C12N9/1252 , C12N9/22 , C12Q1/683 , Y10T436/143333 , C12Q2561/109
摘要： The present invention provides novel cleavage agents and polymerases for the cleavage and modification of nucleic acid. The cleavage agents and polymerases find use, for example, for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. In some embodiments, the 5′ nuclease activity of a variety of enzymes is used to cleave a target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
摘要翻译： 本发明提供了用于切割和修饰核酸的新型切割剂和聚合酶。 裂解剂和聚合酶可用于例如核酸序列的检测和表征以及核酸序列的变化。 在一些实施方案中，使用各种酶的5'核酸酶活性来切割靶依赖性切割结构，从而指示特异性核酸序列的存在或其特定变体。
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公开(公告)号：US20080293046A1
公开(公告)日：2008-11-27
申请号：US11642815
申请日：2006-12-19
申请人： Hatim Allawi , Brad T. Argue , Christian Tor Bartholomay , LuAnne Chehak , Michelle L. Curtis , Peggy S. Eis , Jeff G. Hall , Hon S. Ip , Lin Ji , Michael Kaiser , Robert W. Kwiatkowski, JR. , Andrew A. Lukowiak , Victor Lyamichev , Natalie E. Lyamicheva , WuPo Ma , Bruce P. Neri , Sarah M. Olson , Marilyn C. Olson-Munoz , James J. Schaefer , Zbigniev Skrzypczynski , Tsetska Y. Takova , Lisa C. Thompson , Kevin L. Vedvik
发明人： Hatim Allawi , Brad T. Argue , Christian Tor Bartholomay , LuAnne Chehak , Michelle L. Curtis , Peggy S. Eis , Jeff G. Hall , Hon S. Ip , Lin Ji , Michael Kaiser , Robert W. Kwiatkowski, JR. , Andrew A. Lukowiak , Victor Lyamichev , Natalie E. Lyamicheva , WuPo Ma , Bruce P. Neri , Sarah M. Olson , Marilyn C. Olson-Munoz , James J. Schaefer , Zbigniev Skrzypczynski , Tsetska Y. Takova , Lisa C. Thompson , Kevin L. Vedvik
CPC分类号： C12Q1/6823 , C12N9/1252 , C12N9/22 , C12Q1/683 , Y10T436/143333 , C12Q2561/109
摘要： The present invention provides novel cleavage agents and polymerases for the cleavage and modification of nucleic acid. The cleavage agents and polymerases find use, for example, for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. In some embodiments, the 5′ nuclease activity of a variety of enzymes is used to cleave a target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
摘要翻译： 本发明提供了用于切割和修饰核酸的新型切割剂和聚合酶。 裂解剂和聚合酶可用于例如核酸序列的检测和表征以及核酸序列的变化。 在一些实施方案中，使用各种酶的5'核酸酶活性来切割靶依赖性切割结构，从而指示特异性核酸序列的存在或其特定变体。
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公开(公告)号：US08063184B2
公开(公告)日：2011-11-22
申请号：US12898490
申请日：2010-10-05
申请人： Hatim Allawi , Brad T. Argue , Christian Tor Bartholomay , LuAnne Chehak , Michelle L. Curtis , Peggy S. Eis , Jeff G. Hall , Hon S. Ip , Lin Ji , Michael Kaiser , Robert W. Kwiatkowski, Jr. , Andrew A. Lukowiak , Victor Lyamichev , Natalie E. Lyamicheva , WuPo Ma , Bruce P. Neri , Sarah M. Olson , Marilyn C. Olson-Munoz , James J. Schaefer , Zbigniev Skrzypczynski , Tsetska Y. Takova , Lisa C. Thompson , Kevin L. Vedvik
发明人： Hatim Allawi , Brad T. Argue , Christian Tor Bartholomay , LuAnne Chehak , Michelle L. Curtis , Peggy S. Eis , Jeff G. Hall , Hon S. Ip , Lin Ji , Michael Kaiser , Robert W. Kwiatkowski, Jr. , Andrew A. Lukowiak , Victor Lyamichev , Natalie E. Lyamicheva , WuPo Ma , Bruce P. Neri , Sarah M. Olson , Marilyn C. Olson-Munoz , James J. Schaefer , Zbigniev Skrzypczynski , Tsetska Y. Takova , Lisa C. Thompson , Kevin L. Vedvik
CPC分类号： C12Q1/6823 , C12N9/1252 , C12N9/22 , C12Q1/683 , Y10T436/143333 , C12Q2561/109
摘要： The present invention provides novel cleavage agents and polymerases for the cleavage and modification of nucleic acid. The cleavage agents and polymerases find use, for example, for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. In some embodiments, the 5′ nuclease activity of a variety of enzymes is used to cleave a target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
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公开(公告)号：US07582436B2
公开(公告)日：2009-09-01
申请号：US11787843
申请日：2007-04-18
申请人： Jeff G. Hall , Zbigniev Skrzypczynski , Sarah Wayland , Ned D. Reimer , Luis P. Reynaldo , Joerg Baier , Victor Lyamichev , Bruce P. Neri
发明人： Jeff G. Hall , Zbigniev Skrzypczynski , Sarah Wayland , Ned D. Reimer , Luis P. Reynaldo , Joerg Baier , Victor Lyamichev , Bruce P. Neri
CPC分类号： C12Q1/6823 , C12Q1/6806 , C12Q1/6853 , C12Q2561/109 , C12Q2525/197
摘要： The present invention provides compositions comprising oligonucleotides that have 3′ end groups (e.g. lipophilic moieties) that are useful in invasive cleavage reactions such as the INVADER assay. Specifically, the present invention provides compositions containing oligonucleotides with 3′ end groups configured for generating a detectable signal in invasive cleavages assays with a high signal-to-background ratio, as well as methods for generating such compositions.
摘要翻译： 本发明提供了包含具有3'端基（例如亲脂部分）的寡核苷酸的组合物，其可用于浸润性切割反应，例如INVADER测定。 具体而言，本发明提供含有3'末端基团的寡核苷酸的组合物，其配置用于在具有高信号与背景比的侵入性切割分析中产生可检测信号，以及产生这种组合物的方法。
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公开(公告)号：US20080293930A1
公开(公告)日：2008-11-27
申请号：US11787843
申请日：2007-04-18
申请人： Jeff G. Hall , Zbigniev Skrzypczynski , Sarah Wayland , Ned D. Reimer , Luis P. Reynaldo , Joerg Baier , Victor Lyamichev , Bruce P. Neri
发明人： Jeff G. Hall , Zbigniev Skrzypczynski , Sarah Wayland , Ned D. Reimer , Luis P. Reynaldo , Joerg Baier , Victor Lyamichev , Bruce P. Neri
IPC分类号： C07H1/00
CPC分类号： C12Q1/6823 , C12Q1/6806 , C12Q1/6853 , C12Q2561/109 , C12Q2525/197
摘要： The present invention provides compositions comprising oligonucleotides that have 3′ end groups (e.g. lipophilic moieties) that are useful in invasive cleavage reactions such as the INVADER assay. Specifically, the present invention provides compositions containing oligonucleotides with 3′ end groups configured for generating a detectable signal in invasive cleavages assays with a high signal-to-background ratio, as well as methods for generating such compositions.
摘要翻译： 本发明提供了包含具有3'端基（例如亲脂部分）的寡核苷酸的组合物，其可用于浸润性切割反应，例如INVADER测定。 具体而言，本发明提供含有3'末端基团的寡核苷酸的组合物，其配置用于在具有高信号与背景比的侵入性切割分析中产生可检测信号，以及产生这种组合物的方法。
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公开(公告)号：US20050074788A1
公开(公告)日：2005-04-07
申请号：US10740256
申请日：2003-12-18
申请人： James Dahlberg , Hatim Allawi , Victor Lyamichev , Bruce Neri , Marilyn Olson-Munoz , LuAnne Chehak , Sarah Olson
发明人： James Dahlberg , Hatim Allawi , Victor Lyamichev , Bruce Neri , Marilyn Olson-Munoz , LuAnne Chehak , Sarah Olson
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6816 , C12Q2565/1015 , C12Q2561/109 , C12Q2525/207
摘要： The present invention relates to compositions and methods for the detection and characterization of interfering RNAs such as micro RNAs (miRNAs) and small interfering RNAs (siRNAs) and other short nucleic acid molecules. More particularly, the present invention relates to improved methods for the detection and quantitation of interfering RNA expression. The present invention further provides for the detection of variants and types of miRNAs and siRNAs.
摘要翻译： 本发明涉及用于检测和表征干扰RNA如微RNA（miRNA）和小干扰RNA（siRNA）和其他短核酸分子的组合物和方法。 更具体地，本发明涉及用于检测和定量干扰RNA表达的改进方法。 本发明还提供了miRNA和siRNA的变体和类型的检测。
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公开(公告)号：US20050048527A1
公开(公告)日：2005-03-03
申请号：US10783557
申请日：2004-02-20
申请人： Hatim Allawi , Michael Kaiser , Wu-Po Ma , Bruce Neri , Victor Lyamichev
发明人： Hatim Allawi , Michael Kaiser , Wu-Po Ma , Bruce Neri , Victor Lyamichev
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6823 , C12Q2521/301
摘要： The present invention provides novel cleavage agents for the cleavage and modification of nucleic acid. The cleavage agents find use, for example, for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. In some embodiments, an endonuclease activity is used to cleave a target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
摘要翻译： 本发明提供了用于切割和修饰核酸的新的切割剂。 裂解剂可用于例如核酸序列的检测和表征以及核酸序列的变化。 在一些实施方案中，使用内切核酸酶活性来切割靶依赖性切割结构，从而指示特异性核酸序列的存在或其特定变体。
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公开(公告)号：US20060147955A1
公开(公告)日：2006-07-06
申请号：US11266723
申请日：2005-11-03
申请人： Hatim Allawi , Victor Lyamichev , Vecheslav Elagin , Scott Law , Jeff Hall
发明人： Hatim Allawi , Victor Lyamichev , Vecheslav Elagin , Scott Law , Jeff Hall
CPC分类号： C12Q1/6823 , C12Q2561/109 , C12Q2547/101 , C12Q2531/113
摘要： The present invention provides methods and routines for developing and optimizing nucleic acid detection assays for use in basic research, clinical research, and for the development of clinical detection assays. In particular, the present invention provides methods for designing oligonucleotide primers to be used in multiplex amplification reactions. The present invention also provides methods to optimize multiplex amplification reactions. The present invention also provides methods for combined target and signal generation assays.
摘要翻译： 本发明提供用于开发和优化用于基础研究，临床研究和用于开发临床检测测定的核酸检测测定的方法和程序。 特别地，本发明提供了用于多重扩增反应中使用的寡核苷酸引物的设计方法。 本发明还提供优化多重扩增反应的方法。 本发明还提供了用于组合的靶和信号产生测定的方法。
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10.发明申请Compositions and methods for increased dynamic range detection of nucleic acids 审中-公开用于增加核酸动态范围检测的组合物和方法公开(公告)号：US20060246475A1
公开(公告)日：2006-11-02
申请号：US11338244
申请日：2006-01-23
申请人： Patrick Peterson , Hatim Allawi , Victor Lyamichev , Scott Law , Vecheslav Elagin , Jeff Hall
发明人： Patrick Peterson , Hatim Allawi , Victor Lyamichev , Scott Law , Vecheslav Elagin , Jeff Hall
CPC分类号： C12Q1/6816 , C12Q1/6818 , C12Q1/6827 , C12Q2561/109 , C12Q2537/143 , C12Q2527/143 , C12Q2525/204 , C12Q2525/161 , C12Q2525/301 , C12Q2565/1015
摘要： The present invention provides systems, methods and kits for increasing the dynamic range of detection of a target nucleic acid in a sample. In particular, the present invention provides methods and kits for increasing the dynamic range of detection of a target nucleic acid in a sample through the use of one or more probe oligonucleotides (e.g., analyte-specific probe oligonucleotides).
摘要翻译： 本发明提供用于增加样品中靶核酸的动态检测范围的系统，方法和试剂盒。 特别地，本发明提供了通过使用一种或多种探针寡核苷酸（例如分析物特异性探针寡核苷酸）增加样品中靶核酸的动态检测范围的方法和试剂盒。
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