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21 条记录 (耗时 0.021 秒)

    METHODS OF ENHANCING AND EXPEDITING EXPRESSION OF ANTIBODIES
    5.
    发明公开
    METHODS OF ENHANCING AND EXPEDITING EXPRESSION OF ANTIBODIES 审中-公开

    公开(公告)号：US20240271127A1

    公开(公告)日：2024-08-15

    申请号：US17784796

    申请日：2020-12-15

    申请人： Aridis Pharmaceuticals, Inc.

    发明人： Kathy Ngo ,  Jennifer Woo ,  Binh Pham ,  Vu Truong-Le

    IPC分类号： C12N15/11 ,  C07K16/00 ,  C12N5/071 ,  C12N9/22

    CPC分类号： C12N15/111 ,  C07K16/00 ,  C12N5/0682 ,  C12N9/22 ,  C07K2317/14 ,  C12N2310/20

    摘要： This application describes cells, systems, and molecular engineering methods using CRISPR/Cas complexes for targeted activation of endogenous master transcriptional regulatory elements (MTRE) such as PRDM1, XBP1 and IRF4, to generate high productivity antibody production in production cell lines such as CHO and NSO cells. These incorporate the inclusion of the Cas accessory proteins, design of multiple guide RNAs (gRNA), and unique multiplexing of these components using, e.g., lentiviral transfection to induce increased transcription and translation of antibody genes under the control of the MTRE. The methods result in synergies increasing monoclonal antibody production by these modified cell lines. While a significant increase in productivity is demonstrated by this method of activation, further increase in productivity can be accomplished by genetic transfer of additional copies of MTREs.

    Human monoclonal antibody specific for the F protein of respiratory syncytial virus (RSV)
    8.
    发明授权
    Human monoclonal antibody specific for the F protein of respiratory syncytial virus (RSV) 有权
    呼吸道合胞病毒（RSV）F蛋白特异性的人单克隆抗体

    公开(公告)号：US09340604B1

    公开(公告)日：2016-05-17

    申请号：US14527545

    申请日：2014-10-29

    申请人： Aridis Pharmaceuticals, Inc.

    发明人： Holger Koch ,  Simon Urwyler ,  Michael P. Rudolf ,  Vu L. Truong

    IPC分类号： A61K39/42 ,  C07K16/00 ,  C07K16/10

    CPC分类号： C07K16/1027 ,  C07K2317/21 ,  C07K2317/76 ,  C07K2317/92

    摘要： This invention is directed to an antibody construct or fragment thereof derived from an RSV-infected human, such that the antibody construct binds with specificity to RSV fusion protein antigenic region II/A with an affinity of greater than 1×10−9 M. Preferably, the antibody construct is capable of neutralizing RSV strains, including at least one RSV strain that is resistant to palivizumab. The invention further relates to nucleic acids encoding the antibody construct or portions thereof, and cell lines expressing the antibody. This invention further relates to methods for producing said antibody construct, and to the use of said antibody construct for treating or preventing infection of a patient by RSV having a normal or mutated version of F protein.

    摘要翻译： 本发明涉及来自RSV感染的人的抗体构建体或其片段，使得抗体构建体以大于1×10 -9 M的亲和力对RSV融合蛋白抗原区II / A具有特异性结合。优选地 抗体构建体能够中和RSV菌株，包括至少一种对帕利珠单抗有抗性的RSV菌株。 本发明还涉及编码抗体构建体或其部分的核酸和表达抗体的细胞系。 本发明还涉及用于产生所述抗体构建体的方法，以及所述抗体构建体用于治疗或预防具有正常或突变型F蛋白的RSV感染患者的方法。

    Sonic low pressure spray drying
    9.
    发明授权
    Sonic low pressure spray drying 有权
    声波低压喷雾干燥

    公开(公告)号：US08673357B2

    公开(公告)日：2014-03-18

    申请号：US13585096

    申请日：2012-08-14

    申请人： Vu Truong-Le ,  Satoshi Ohtake ,  Russell Andrew Martin ,  Binh V. Pham ,  Luisa Yee

    发明人： Vu Truong-Le ,  Satoshi Ohtake ,  Russell Andrew Martin ,  Binh V. Pham ,  Luisa Yee

    IPC分类号： A61K9/14 ,  F26B5/06

    CPC分类号： B01D1/18

    摘要： This invention provides methods of spray drying pharmaceutical powders from a vibrating nozzle at low pressures. The method can effectively spray dry thick or viscous solutions or suspensions to provide small uniform particles. The invention includes dry particle compositions prepared by methods of low pressure spraying from vibrating nozzles.

    摘要翻译： 本发明提供了在低压下从振动喷嘴喷雾干燥药物粉末的方法。 该方法可以有效地喷洒干燥的稠粘或粘稠溶液或悬浮液以提供小的均匀颗粒。 本发明包括通过从振动喷嘴进行低压喷射的方法制备的干燥颗粒组合物。