公开(公告)号：US09175421B2

公开(公告)日：2015-11-03

申请号：US13332411

申请日：2011-12-21

申请人： Ariel Notcovich ,  Alon Herschhorn ,  Shai Nimri ,  John Barich ,  Ariel Lipson ,  Ran Boaz ,  Doron Lipson ,  Yaakov Levie

发明人： Ariel Notcovich ,  Alon Herschhorn ,  Shai Nimri ,  John Barich ,  Ariel Lipson ,  Ran Boaz ,  Doron Lipson ,  Yaakov Levie

IPC分类号： C40B30/04 ,  G01N21/552 ,  G01N21/64 ,  G01N33/543 ,  G01N33/557 ,  B01L3/00 ,  G01N21/05 ,  G01N21/21 ,  G01N35/00 ,  G01N21/03

CPC分类号： C40B30/04 ,  B01J2219/00657 ,  B01J2219/00659 ,  B01L3/5025 ,  B01L3/5027 ,  B01L3/502715 ,  B01L2300/0636 ,  B01L2300/0877 ,  G01N21/05 ,  G01N21/553 ,  G01N21/648 ,  G01N33/54373 ,  G01N33/5438 ,  G01N33/557 ,  G01N2021/0346 ,  G01N2021/212 ,  G01N2021/6439 ,  G01N2021/6463 ,  G01N2035/00158

摘要： Provided is a method for determining one or more kinetic parameters of binding between a first binding member and a second binding member. The method includes adsorbing the first binding member to a surface at a plurality of microspots. The second binding member is then presented to the first binding member at each of the microspots, there being a plurality of combinations of first binding member surface density and second binding member concentration among the plurality of microspots. Data indicative of a binding reaction between the first of microspots are then obtained and analyzed so as to obtain one or more kinetic parameters of the binding between the first and second binding members. Also provided is a system for carrying out the method. A method for localizing a molecular species at microspots on a surface, and a probe array produced by the method are also provided.

摘要翻译： 提供了一种用于确定第一结合构件和第二结合构件之间的结合的一个或多个动力学参数的方法。 该方法包括将第一结合构件吸附到多个微点的表面。 然后将第二结合构件提供给每个微点处的第一结合构件，在多个微孔中存在多个第一结合构件表面密度和第二结合成员浓度的组合。 然后获得并分析指示第一个微孔之间的结合反应的数据，以便获得第一和第二结合成员之间的结合的一个或多个动力学参数。 还提供了一种用于执行该方法的系统。 还提供了一种用于定位表面上的微孔分子种类的方法，以及通过该方法制备的探针阵列。

公开(公告)号：US09040309B2

公开(公告)日：2015-05-26

申请号：US11471716

申请日：2006-06-21

申请人： Shai Nimri

发明人： Shai Nimri

IPC分类号： G01N33/548 ,  G01N33/543

CPC分类号： G01N33/54353 ,  G01N33/548

摘要： The invention provides a binding layer comprising a polysaccharide substituted by carboxylic groups or derivatives thereof exhibiting high performance in the binding of ligand molecules and in the interaction thereof with analyte molecules. A method for the preparation of the binding layer and for the assaying of various analyte molecules is also provided.

摘要翻译： 本发明提供一种结合层，其包含被羧基或其衍生物取代的多糖，其在配体分子的结合和与分析物分子的相互作用中表现出高性能。 还提供了制备结合层和分析各种分析物分子的方法。

公开(公告)号：US20120129723A1

公开(公告)日：2012-05-24

申请号：US13332411

申请日：2011-12-21

申请人： Ariel NOTCOVICH ,  Alon HERSCHHORN ,  Shai NIMRI ,  John BARICH ,  Ariel LIPSON ,  Ran BOAZ ,  Doron LIPSON ,  Yaakov LEVIE

发明人： Ariel NOTCOVICH ,  Alon HERSCHHORN ,  Shai NIMRI ,  John BARICH ,  Ariel LIPSON ,  Ran BOAZ ,  Doron LIPSON ,  Yaakov LEVIE

IPC分类号： C40B30/04 ,  C40B60/08

CPC分类号： C40B30/04 ,  B01J2219/00657 ,  B01J2219/00659 ,  B01L3/5025 ,  B01L3/5027 ,  B01L3/502715 ,  B01L2300/0636 ,  B01L2300/0877 ,  G01N21/05 ,  G01N21/553 ,  G01N21/648 ,  G01N33/54373 ,  G01N33/5438 ,  G01N33/557 ,  G01N2021/0346 ,  G01N2021/212 ,  G01N2021/6439 ,  G01N2021/6463 ,  G01N2035/00158

摘要： Provided is a method for determining one or more kinetic parameters of binding between a first binding member and a second binding member. The method includes adsorbing the first binding member to a surface at a plurality of microspots. The second binding member is then presented to the first binding member at each of the microspots, there being a plurality of combinations of first binding member surface density and second binding member concentration among the plurality of microspots. Data indicative of a binding reaction between the first of microspots are then obtained and analyzed so as to obtain one or more kinetic parameters of the binding between the first and second binding members. Also provided is a system for carrying out the method. A method for localizing a molecular species at microspots on a surface, and a probe array produced by the method are also provided.

摘要翻译： 提供了一种用于确定第一结合构件和第二结合构件之间的结合的一个或多个动力学参数的方法。 该方法包括将第一结合构件吸附到多个微点的表面。 然后将第二结合构件提供给每个微点处的第一结合构件，在多个微孔中存在多个第一结合构件表面密度和第二结合成员浓度的组合。 然后获得并分析指示第一个微孔之间的结合反应的数据，以便获得第一和第二结合成员之间的结合的一个或多个动力学参数。 还提供了一种用于执行该方法的系统。 还提供了一种用于定位表面上的微孔分子种类的方法，以及通过该方法制备的探针阵列。

公开(公告)号：US20140212990A1

公开(公告)日：2014-07-31

申请号：US14167428

申请日：2014-01-29

申请人： Shai NIMRI ,  Vered BRONNER

发明人： Shai NIMRI ,  Vered BRONNER

IPC分类号： G01N33/53

摘要： The present invention is directed to novel assays for detecting target molecules. The assays employ small size, detectably labeled, magnetic nanoparticles associated with a capture molecule. The detection assay is accelerated by applying magnetic field during the assay. The assays of the invention can be used to enhance the efficiency of the detection step in dot blot, Western blot and ELISA.

摘要翻译： 本发明涉及用于检测靶分子的新型测定法。 测定采用与捕获分子相关的小尺寸，可检测标记的磁性纳米颗粒。 在测定期间通过施加磁场来加速检测测定。 本发明的测定法可用于提高斑点印迹，Western印迹和ELISA检测步骤的效率。

公开(公告)号：US08105845B2

公开(公告)日：2012-01-31

申请号：US10578860

申请日：2004-11-14

申请人： Ariel Notcovich ,  Alon Herschhorn ,  Shai Nimri ,  John Barich ,  Ariel Lipson ,  Ran Boaz ,  Doron Lipson ,  Yaakov Levie

发明人： Ariel Notcovich ,  Alon Herschhorn ,  Shai Nimri ,  John Barich ,  Ariel Lipson ,  Ran Boaz ,  Doron Lipson ,  Yaakov Levie

IPC分类号： G01N33/543

CPC分类号： C40B30/04 ,  B01J2219/00657 ,  B01J2219/00659 ,  B01L3/5025 ,  B01L3/5027 ,  B01L3/502715 ,  B01L2300/0636 ,  B01L2300/0877 ,  G01N21/05 ,  G01N21/553 ,  G01N21/648 ,  G01N33/54373 ,  G01N33/5438 ,  G01N33/557 ,  G01N2021/0346 ,  G01N2021/212 ,  G01N2021/6439 ,  G01N2021/6463 ,  G01N2035/00158

摘要： A method for determining one or more kinetic parameters of binding between a first binding member and a second binding member. The method comprises adsorbing the first binding member to a surface at a plurality of microspots. The second binding member is then presented to the first binding member at each of the microspots, there being a plurality of combinations of first binding member surface density and second binding member concentration among the plurality of microspots. Data indicative of a binding reaction between the first of microspots are then obtained and analyzed so as to obtain one or more kinetic parameters of the binding between the first and second binding members. The invention also provides a system for carrying out the method. A method for localizing a molecular species at microspots on a surface, and a probe array produced by the method are also provided.

摘要翻译： 一种用于确定第一结合构件和第二结合构件之间的结合的一个或多个动力学参数的方法。 该方法包括将第一结合构件吸附到多个微点处的表面。 然后将第二结合构件提供给每个微点处的第一结合构件，在多个微孔中存在多个第一结合构件表面密度和第二结合成员浓度的组合。 然后获得并分析指示第一个微孔之间的结合反应的数据，以便获得第一和第二结合成员之间的结合的一个或多个动力学参数。 本发明还提供一种用于执行该方法的系统。 还提供了一种用于定位表面上的微孔分子种类的方法，以及通过该方法制备的探针阵列。

公开(公告)号：US10732178B2

公开(公告)日：2020-08-04

申请号：US14167428

申请日：2014-01-29

申请人： Bio-Rad HAIFA Ltd.

发明人： Shai Nimri ,  Vered Bronner

IPC分类号： G01N33/543 ,  G01N33/53

摘要： The present invention is directed to novel assays for detecting target molecules. The assays employ small size, detectably labeled, magnetic nanoparticles associated with a capture molecule. The detection assay is accelerated by applying magnetic field during the assay. The assays of the invention can be used to enhance the efficiency of the detection step in dot blot, Western blot and ELISA.

公开(公告)号：US20080198384A1

公开(公告)日：2008-08-21

申请号：US12149158

申请日：2008-04-28

申请人： Boaz Ran ,  Ariel G. Notcovich ,  Ariel Lipson ,  Shay Nimri ,  Stephen G. Lipson ,  Doron Lipson

发明人： Boaz Ran ,  Ariel G. Notcovich ,  Ariel Lipson ,  Shay Nimri ,  Stephen G. Lipson ,  Doron Lipson

IPC分类号： G01N21/55

CPC分类号： G01N21/55 ,  G01N21/553 ,  G01N2021/058

摘要： An SPR sensor comprising: a thin conducting layer comprising at least one conductive element formed on a surface of a transparent substrate; an illumination system controllable to illuminate an interface between the conducting layer and the substrate; a photosensitive surface that generates signals responsive to light from the light source that is reflected from a region of the interface; a flow cell formed with at least one flow channel having a lumen defined by a wall at least a portion of which is formed from an elastic material and a portion of which is formed by a region of the conducting layer; and at least one hollow needle having an exit orifice communicating with the needle's lumen and wherein fluid flow is enabled between the flow channel and the needle's lumen by puncturing the elastic material with the at least one needle so that the exit orifice communicates with the flow channel lumen.