公开(公告)号：US5521084A

公开(公告)日：1996-05-28

申请号：US975719

申请日：1992-11-10

申请人： Jacek Kowalski ,  Scott Gilbert ,  Timothy J. Zamb

发明人： Jacek Kowalski ,  Scott Gilbert ,  Timothy J. Zamb

IPC分类号： C12N15/09 ,  A61K39/00 ,  C07K14/03 ,  C12N1/21 ,  C12N5/10 ,  C12N15/38 ,  C12N15/40 ,  C12N15/85 ,  C12P21/00 ,  C12N15/11

CPC分类号： C07K14/005 ,  C12N15/85 ,  A61K39/00 ,  C07K2319/02 ,  C12N2710/16722 ,  C12N2830/002 ,  C12N2830/85

摘要： A novel expression system using the heat-inducible bovine hsp70A promoter and associated cis-acting elements is disclosed. The system provides for the continuous production of a highly pure, authentic protein, substantially free of infectious viral and cellular protein contaminants.

公开(公告)号：US5494801A

公开(公告)日：1996-02-27

申请号：US162401

申请日：1993-12-03

申请人： Gregory R. Bogart ,  Robert J. Bilodeau ,  Rachel M. Ostroff ,  Jeffrey W. Steaffens

发明人： Gregory R. Bogart ,  Robert J. Bilodeau ,  Rachel M. Ostroff ,  Jeffrey W. Steaffens

IPC分类号： G01N33/569

CPC分类号： A61K39/092 ,  G01N33/56944 ,  Y10S435/961 ,  Y10S435/962 ,  Y10S435/975 ,  Y10S436/808 ,  Y10T436/25 ,  Y10T436/25125

摘要： Method for extracting an antigen from a micro-organism organism of the genus streptococcus contained in a sample including the steps of contacting the sample with reagents which generate hyponitrous acid, further contacting the sample with a base, or contacting the sample with hypochlorite, and neutralizing the sample.

摘要翻译： 从样品中含有的链球菌属微生物生物体提取抗原的方法，包括使样品与产生亚硝酸的试剂接触的步骤，进一步使样品与碱接触或使样品与次氯酸盐接触，中和 例子。

公开(公告)号：US5468606A

公开(公告)日：1995-11-21

申请号：US923304

申请日：1992-07-31

申请人： Gregory R. Bogart ,  Garret R. Moddel ,  Diana M. Maul ,  Jeffrey B. Etter

发明人： Gregory R. Bogart ,  Garret R. Moddel ,  Diana M. Maul ,  Jeffrey B. Etter

IPC分类号： G01N21/21 ,  G01N21/45 ,  G01N21/47 ,  G01N21/77 ,  G01N33/543 ,  G01N33/551 ,  G01N33/552 ,  G01N33/545

CPC分类号： B82Y30/00 ,  B82Y15/00 ,  G01N21/211 ,  G01N21/4738 ,  G01N21/77 ,  G01N33/54366 ,  G01N33/54373 ,  G01N33/551 ,  G01N33/552 ,  G01N21/45 ,  Y10S435/808 ,  Y10S436/805

摘要： Device for detecting the presence or amount of an analyte of interest, having a substrate possessing an optically active surface which exhibits a first color in response to light impinging thereon, and exhibits a second color comprising a combination of wavelengths of light different from the first color or comprising an intensity of at least one wavelength of light different from the first color, in response to the light when the analyte is present on the surface in any amount selected from 0.1 nM, 0.1 ng/ml, 50 fg, and 2.times.10.sup.3 organisms comprising the analyte.

摘要翻译： 用于检测感兴趣的分析物的存在或量的装置，具有具有光学活性表面的基底，所述基底表现出响应于其上的光的第一颜色，并且显示第二颜色，其包括与第一颜色不同的光的波长的组合 或者当所述分析物以选自0.1nM，0.1ng / ml，50fg和2x103生物体的任何量存在于所述表面上时，响应于所述光而包含至少一种不同于所述第一颜色的波长的光的强度，所述生物体包含 分析物。

公开(公告)号：US20020192664A1

公开(公告)日：2002-12-19

申请号：US09982658

申请日：2001-10-18

申请人： THERMO BIOSTAR, INC.

发明人： Hakan Nygren ,  Manne Johan E. Stenberg

IPC分类号： C12Q001/68 ,  C12M001/34

CPC分类号： B82Y30/00 ,  C12Q1/6825 ,  G01N33/526 ,  Y10S436/805

摘要： An optical device for determining the presence of a first nucleic acid in a sample comprising a second nucleic acid complementary to the first nucleic acid and able to hybridize with the first nucleic acid under hybridizing conditions, the second nucleic acid being bonded to a solid support, wherein the solid support is formed as a light reflecting surface having a first thickness when bonded to the second nucleic acid, and wherein the light reflecting surface has a second thickness, wherein the first and second nucleic acids are hybridized, and the first and second thicknesses can be distinguished by their effect on the light reflecting properties of said light reflecting surface independent of any label present on the first nucleic acid.

公开(公告)号：US6060237A

公开(公告)日：2000-05-09

申请号：US375151

申请日：1995-01-17

申请人： Hakan Nygren ,  Manne Stenberg

发明人： Hakan Nygren ,  Manne Stenberg

IPC分类号： C12Q1/68 ,  G01N33/52 ,  C07H21/04 ,  G01J4/00

CPC分类号： B82Y30/00 ,  C12Q1/6825 ,  G01N33/526 ,  Y10S436/805

摘要： An optical device for determining the presence of a first nucleic acid in a sample comprising a second nucleic acid complementary to the first nucleic acid and able to hybridize with the first nucleic acid under hybridizing conditions, the second nucleic acid being bonded to a solid support, wherein the solid support is formed as a light reflecting surface having a first thickness when bonded to the second nucleic acid, and wherein the light reflecting surface has a second thickness, wherein the first and second nucleic acids are hybridized, and the first and second thicknesses can be distinguished by their effect on the light reflecting properties of said light reflecting surface independent of any label present on the first nucleic acid.

公开(公告)号：US5709865A

公开(公告)日：1998-01-20

申请号：US445746

申请日：1995-05-22

申请人： Jan van den Hurk ,  Peter Tijssen

发明人： Jan van den Hurk ,  Peter Tijssen

IPC分类号： A61K39/00 ,  A61K39/12 ,  C07K14/18 ,  C07H21/02 ,  C07K1/00 ,  C12N15/00

CPC分类号： C07K14/005 ,  A61K39/12 ,  A61K2039/552 ,  A61K2039/55566 ,  A61K39/00 ,  C12N2770/24322 ,  C12N2770/24334

摘要： This invention relates to the identification of Bovine Viral Diarrhea Virus group II (BVDV-II) nucleic acid sequences (e.g., gp53 sequences), to methods of using the nucleic acid sequences for detecting BVD-II virus in animal sera, to polypeptide vital antigens derived from the sequences and immunoreactive with sera from animals infected with Bovine Viral Diarrhea group II (BVD-II) virus, to polynucleotide sequences which encode these polypeptide antigens, to an expression system capable of producing the polypeptide antigens, to vaccines containing the polypeptide antigens, to methods of using the polypeptide antigens for detecting BVD-II virus antibodies in animal sera, and to antibodies directed against these polypeptide antigens.

摘要翻译： 本发明涉及将牛病毒性腹泻病毒组II（BVDV-II）核酸序列（例如，gp53序列）鉴定为使用核酸序列检测动物血清中的BVD-II病毒的方法来多肽重要抗原 衍生自序列并且与来自感染牛病毒性腹泻组II（BVD-II）病毒的动物的血清对编码这些多肽抗原的多核苷酸序列与能够产生多肽抗原的表达系统进行免疫反应）到含有多肽抗原的疫苗 涉及使用多肽抗原检测动物血清中BVD-II病毒抗体的方法，以及针对这些多肽抗原的抗体。

公开(公告)号：US5629214A

公开(公告)日：1997-05-13

申请号：US456040

申请日：1995-05-31

申请人： Mark Crosby

发明人： Mark Crosby

IPC分类号： G01N21/21 ,  G01N21/77 ,  G01N33/543 ,  G01N33/551 ,  G01N33/552 ,  G01N33/569 ,  G01N33/545 ,  G01N33/547

CPC分类号： G01N21/78 ,  G01N21/21 ,  G01N21/211 ,  G01N21/6428 ,  G01N21/77 ,  G01N33/54366 ,  G01N33/54373 ,  G01N33/54386 ,  G01N33/551 ,  G01N33/552 ,  G01N33/56927 ,  B82Y30/00 ,  B82Y35/00 ,  G01N2021/7779 ,  Y10S435/808 ,  Y10S435/81 ,  Y10S436/804 ,  Y10S436/805 ,  Y10S436/811 ,  Y10T436/25 ,  Y10T436/25375

摘要： A method for forming an optical device for detecting the presence or amount of an analyte of interest comprising a substrate which supports an optically active layer, an attachment layer provided on the optically active layer, and a receptive layer specific the analyte provided on the attachment layer. The method comprises forming the optically active layer with a chosen refractive index on the substrate by curing the optically active layer on the substrate at a controlled temperature or for a controlled length of time and subsequently providing the attachment and receptive layers on the optically active layer.

摘要翻译： 一种用于形成用于检测感兴趣分析物的存在或量的光学装置的方法，包括：支撑光学活性层的基底，设置在光学活性层上的附着层，以及特异性地提供在附着层上的分析物 。 该方法包括通过在受控的温度或受控的时间长度固化衬底上的光学活性层，并且随后在光学活性层上提供附着和接受层，在衬底上形成具有选定折射率的光学活性层。

公开(公告)号：US5552272A

公开(公告)日：1996-09-03

申请号：US76348

申请日：1993-06-10

申请人： Gregory R. Bogart

发明人： Gregory R. Bogart

IPC分类号： G01N33/543 ,  G01N33/58

CPC分类号： B82Y30/00 ,  B82Y15/00 ,  G01N33/54353 ,  G01N33/582 ,  Y10S435/808 ,  Y10S435/81 ,  Y10S436/805 ,  Y10S436/807

摘要： Device for detecting the presence or amount of an analyte of interest, comprising a reflective solid, optical support and a label capable of generating fluorescent signal upon excitation with a suitable light source wherein said support comprises an attachment layer comprising a chemical selected from the group consisting of dendrimers, star polymers, molecular self-assembling polymers, polymeric siloxanes, and film forming latexes wherein the support provides an enhanced level of exciting photons to the immobilized fluorescent label compound, and wherein the support also increases the capture of fluorescent signal.

摘要翻译： 用于检测感兴趣分析物的存在或量的装置，包括反射固体，光学载体和能够在用合适的光源激发时产生荧光信号的标签，其中所述载体包括附着层，所述附着层包含选自以下的化学物质： 树枝状聚合物，星形聚合物，分子自组装聚合物，聚合物硅氧烷和成膜胶乳，其中载体提供增强水平的激发光子到固定的荧光标记化合物，并且其中载体也增加荧光信号的捕获。

公开(公告)号：US5494829A

公开(公告)日：1996-02-27

申请号：US75128

申请日：1993-06-10

申请人： Torbjorn Sandstrom ,  Lars Stiblert ,  Diana M. Maul

发明人： Torbjorn Sandstrom ,  Lars Stiblert ,  Diana M. Maul

IPC分类号： G01N21/41 ,  G01N21/77 ,  G01N33/543 ,  G01N33/569 ,  G01N33/551

CPC分类号： G01N21/21 ,  G01N21/4133 ,  G01N21/77 ,  G01N33/54366 ,  G01N33/54386 ,  G01N33/56911 ,  G01N33/56927 ,  G01N33/56944 ,  G01N33/56983 ,  G01N33/56988 ,  Y10S435/808 ,  Y10S436/805

摘要： Instrument configured and arranged to detect the presence or amount of an analyte of interest on the substrate of an optical device. The instrument has a source of linearly polarized, monochromatic light positioned at an angle other than Brewster's angle relative to the substrate; and an analyzer positioned at the same angle relative to the substrate at a location suitable for detecting reflected polarized light from the substrate; wherein the analyzer is configured and arranged to approximately maximize the change in intensity of the light reflected from the substrate that is transmitted through the analyzer when a change in mass occurs at the substrate relative to an unreacted surface.

摘要翻译： 配置和布置用于检测光学装置的基板上的感兴趣分析物的存在或量的仪器。 该仪器具有线性偏振的单色光源，其相对于衬底以不同于布鲁斯特角度的角度定位; 以及在适于检测来自所述衬底的反射偏振光的位置处相对于所述衬底以相同角度定位的分析器; 其中所述分析器被配置和布置成当在所述衬底相对于未反应的表面发生质量变化时，大致最大化从通过所述分析器的衬底反射的光的强度变化。

公开(公告)号：US07153651B1

公开(公告)日：2006-12-26

申请号：US08950963

申请日：1997-10-15

申请人： Joel A. Drewes ,  Gregory R. Bogart ,  Jeffrey B. Etter ,  Jeffrey W. Steaffens ,  Rachel M. Ostroff ,  Mark Crosby

发明人： Joel A. Drewes ,  Gregory R. Bogart ,  Jeffrey B. Etter ,  Jeffrey W. Steaffens ,  Rachel M. Ostroff ,  Mark Crosby

IPC分类号： C12Q1/68 ,  C12M1/00 ,  G01N15/06

CPC分类号： G01N33/54373 ,  G01N21/7703

摘要： An optical assay device for the detection of an analyte of interest in a sample comprising a support containing channels, an optically functional layer positioned on the support such that the optically functional layer and the support allow for laminar flow of the sample through layers of the device, an attachment layer positioned on the optically functional layer, and an analyte specific receptive layer positioned on the attachment layer.

摘要翻译： 一种用于检测样品中感兴趣分析物的光学测定装置，包括含有通道的载体，位于载体上的光学功能层，使得光学功能层和载体允许样品层流流过该装置的层 ，位于光学功能层上的附着层和位于附着层上的分析物特异性接收层。