Multivalent live influenza vaccine platform using recombinant adenovirus

    公开(公告)号:US11672854B2

    公开(公告)日:2023-06-13

    申请号:US16980838

    申请日:2019-01-16

    摘要: The present invention relates to a multivalent live influenza vaccine platform using a recombinant adenovirus. The present invention is a live attenuated vaccine platform using a recombinant virus and it is easy to inoculate because it is infected with the respiratory tract like influenza virus and exhibits a vaccine action and it is a multivalent vaccine which combines two types into one and it is a highly novel vaccine that does not need to mix viruses compared to vaccines using multiple combinations of one vaccine. The present invention is the first vaccine in which a gene obtained by fusion of two influenza antigen genes into one gene is incorporated into a recombinant virus. Instead of using the entire HA gene of influenza, but using a structurally independent HA1 gene, which is about half of the total HA gene, several types of HA genes could be fused into one. When the recombinant virus was inoculated into mice by nasal inhalation, it was confirmed that it is an effective vaccine in which the vaccine effect is induced by two inoculations, and the vaccine platform of the present invention is expected to be useful for the development of a vaccine for human influenza infection.

    MESENCHYMAL STEM CELLS DERIVED FROM SYNOVIUM AND USES THEREOF

    公开(公告)号:US20220112465A1

    公开(公告)日:2022-04-14

    申请号:US17280003

    申请日:2019-09-27

    IPC分类号: C12N5/0775 A61K35/28

    摘要: Provided are a method of preparing synovium-derived mesenchymal stem cells using a synovial tissue and a hydrogel, synovium-derived mesenchymal stem cells prepared by the method, a pharmaceutical composition for treating bone or cartilage damage, the pharmaceutical composition including the synovium-derived mesenchymal stem cells, and a composition and kit for culturing mesenchymal stem cells, the composition and kit including a synovial tissue and a hydrogel. When the preparation method of the present invention is used, stem cells may be effectively extracted and obtained from synovium, and the obtained synovium-derived mesenchymal stem cells may effectively treat bone or cartilage damage, and therefore, it will be able to greatly contribute to the development of a method of treating bone or cartilage damage.