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公开(公告)号:US20230167477A1
公开(公告)日:2023-06-01
申请号:US17982080
申请日:2022-11-07
CPC分类号: C12P21/00 , B01L3/502761 , C12M21/18 , C12M41/32 , C12M47/10 , B01L2400/043 , B01L2400/0427 , B01L2200/04 , B01L2200/0652 , B01L2200/16
摘要: The invention provided herein relates to methods for protein synthesis, characterisation and purification on a digital microfluidic device.
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公开(公告)号:US20230151046A1
公开(公告)日:2023-05-18
申请号:US17916862
申请日:2021-04-06
摘要: The present invention relates to a compound according to Formula (1c) or (1d): wherein, R1; R2; R3 and R6 are defined herein, and their use in methods of nucleic acid synthesis.
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公开(公告)号:US20230128171A1
公开(公告)日:2023-04-27
申请号:US18083053
申请日:2022-12-16
摘要: An electrowetting system is disclosed. The system includes electrodes configured to manipulate droplets of fluid in a microfluidic space. Each electrode is coupled to circuitry operative to selectively apply a driving voltage to the electrode. The system includes a dielectric stack including a first dielectric pair comprising a first layer having a first dielectric constant and a second layer having a second dielectric constant. The second dielectric constant is larger than the first dielectric constant. The dielectric stack includes a second dielectric pair comprising a third layer having a third dielectric constant and a fourth layer having a fourth dielectric constant. The fourth dielectric constant is larger than the third dielectric constant. A ratio of a thickness of the fourth layer to a thickness of the third layer (T4:T3) is in the range from about 2:1 to about 8:1. The second dielectric pair is thinner than the first dielectric pair.
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公开(公告)号:US11353759B2
公开(公告)日:2022-06-07
申请号:US16572022
申请日:2019-09-16
IPC分类号: G02F1/1368 , G02F1/1362 , G02F1/167 , H01L27/12
摘要: Active matrix backplanes including an array of hexagonal electrodes or an array of triangular electrodes. Because the backplane designs route the gate lines along the periphery of the electrodes there is less cross talk with the surface of the electrode. The disclosed designs simplify construction and control of the electrodes and improve the regularity of the electric field above the electrode. Such backplane electrode designs may be particularly useful in electrowetting on dielectric (EWoD) devices and electrophoretic displays (EPD).
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5.
公开(公告)号:US20210394190A1
公开(公告)日:2021-12-23
申请号:US17353031
申请日:2021-06-21
发明人: Richard J. Paolini
IPC分类号: B01L3/00
摘要: A method for holding an aqueous droplet in a selected location within a microfluidic device. The microfluidic device comprises: a top plate comprising a top substrate, a first layer of hydrophobic material applied to a surface of the top substrate, and a common top electrode between the first layer of hydrophobic material and the top substrate; a bottom plate comprising a pixel array, the pixel array comprising a plurality of pixel electrodes and a second layer of hydrophobic material applied over the plurality of pixel electrodes, and a microfluidic gap between the first and second layers of hydrophobic material. The method comprises: applying an intermittent driving pattern to pixels under the area of the droplet. The intermittent driving pattern comprises, in order: actuating a first subset of the pixels under the area of the droplet, and actuating a second subset of the pixels under the area of the droplet.
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公开(公告)号:US20230175030A1
公开(公告)日:2023-06-08
申请号:US17610900
申请日:2020-05-14
摘要: Disclosed an improved process for synthesising a nucleic acid strand using cycles of template independent enzyme extension. Having one or more of the amino groups on the base heterocyclic groups masked with protecting groups helps to prevent secondary structure in the extended strand, thereby improving access of the enzyme to the 3′ OH terminus for extension.
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公开(公告)号:US11623940B2
公开(公告)日:2023-04-11
申请号:US16646361
申请日:2018-09-14
发明人: Michael Chen , Jiahao Huang , Gordon McInroy
IPC分类号: C12Q1/68 , C07H21/00 , C12Q1/6806 , C12P19/34
摘要: The invention relates to the use of polyphosphate containing species in a method of nucleic acid synthesis, to methods of nucleic acid synthesis, and to the use of kits comprising said polyphosphate containing species. The invention also relates to the use of polyphosphate containing species for the capping of 3′-terminal hydroxyl moieties using terminal transferases.
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公开(公告)号:US20230057330A1
公开(公告)日:2023-02-23
申请号:US17965750
申请日:2022-10-13
发明人: Michael Chun Hao Chen , Sumit Kalsi , Laurence Livingstone Bell , Gordon Ross McInroy , David Zhitomirsky , Luke Slominski , Rick Paolini, JR. , Cristina Visani
IPC分类号: B01L3/00
摘要: A method for moving an aqueous droplet comprising providing an electrokinetic device including a first substrate having a matrix of electrodes, wherein each of the matrix electrodes is coupled to a thin film transistor, and wherein the matrix electrodes are overcoated with a functional coating comprising: a dielectric layer in contact with the matrix electrodes, a conformal layer in contact with the dielectric layer, and a hydrophobic layer in contact with the confornial layer; a second substrate comprising a top electrode; a spacer disposed between the first substrate and the second substrate and defining an electrokinetic workspace; and a voltage source operatively coupled to the niatrix electrodes. The method further comprises disposing an aqueous droplet on a first matrix electrode; and providing a differential electrical potential between the first matrix electrode and a second matrix electrode with the voltage source, thereby moving the aqueous droplet.
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公开(公告)号:US11505815B2
公开(公告)日:2022-11-22
申请号:US16191109
申请日:2018-11-14
发明人: Michael C. Chen , Gordon R. McInroy
IPC分类号: C12Q1/68 , C07H19/04 , C07H19/06 , C07H19/16 , C12P19/34 , C07H19/10 , C07H19/20 , C07H1/00 , C12Q1/6844
摘要: The invention relates to the use of an amine masked moiety in a method of enzymatic nucleic acid synthesis. The invention also relates to said amine masked moieties per se and a process for preparing nucleotide triphosphates comprising said amine masked moieties.
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10.
公开(公告)号:US11466301B2
公开(公告)日:2022-10-11
申请号:US16480128
申请日:2018-01-26
发明人: Michael Chen , Jiahao Huang , Radu Lazar , Gordon McInroy
摘要: The invention relates to methods of synthesizing polymers, biopolymers, and nucleic acids, to immobilised dNTP/NTPs and kits comprising said immobilised dNTP/NTPs for use in said methods of nucleic acid synthesis.
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