公开(公告)号：US11920158B2

公开(公告)日：2024-03-05

申请号：US16327355

申请日：2017-08-25

申请人： The Council of the Queensland Institute of Medical Research

发明人： Enzo Porrello ,  James Hudson ,  Drew Titmarsh ,  Richard Mills

IPC分类号： C12N5/077 ,  C12M1/32 ,  C12M1/34 ,  C12Q1/02

CPC分类号： C12N5/0657 ,  C12M23/12 ,  C12M41/00 ,  C12Q1/025 ,  C12N2500/16 ,  C12N2500/34 ,  C12N2500/36 ,  C12N2500/38 ,  C12N2501/115 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/999 ,  C12N2503/02 ,  C12N2506/02 ,  C12N2513/00 ,  C12N2533/54 ,  C12N2533/90

摘要： A cardiomyocyte maturation medium comprises a base medium, one or more fatty acids such as palmitic acid, glucose and albumin. The one or more fatty acids and glucose are ideally present at a concentration ratio of about 1:10. Typically, the medium does not comprise TGF or insulin, or comprises minimal TGF and/or insulin. A cell culture vessel is provided that comprises opposed poles that facilitate force measurements of developing cardiac muscle.

公开(公告)号：US20230210984A1

公开(公告)日：2023-07-06

申请号：US17771375

申请日：2020-10-23

申请人： The Council of the Queensland Institute of Medical Research

发明人： Rajiv KHANNA ,  Debottam SINHA

IPC分类号： A61K39/245 ,  A61P35/00 ,  A61K31/4184 ,  A61P31/22 ,  A61K31/551 ,  A61K31/519

CPC分类号： A61K39/245 ,  A61P35/00 ,  A61K31/4184 ,  A61P31/22 ,  A61K31/551 ,  A61K31/519 ,  A61K2039/5158

摘要： Disclosed herein is the use of a first population of allogeneic T-cells recognizing a first EBV epitope, and a second allogeneic population recognizing a second EBV epitope in the treatment of EBV-associated disorders. Also disclosed is the use of a population of allogeneic T-cells recognizing an EBV antigen in combination with a further therapeutic agent such as an immunotherapeutic agent, a MAPK, BET or MEK. pathway inhibitor for treating EBV-associated disease.

公开(公告)号：US11662343B2

公开(公告)日：2023-05-30

申请号：US16603454

申请日：2018-04-09

申请人： The Council of the Queensland Institute of Medical Research

发明人： Geoffrey R. Hill ,  Ping Zhang

IPC分类号： G01N33/50 ,  C07K14/535 ,  C07K14/54 ,  C07K14/73 ,  C12N5/0783

CPC分类号： G01N33/505 ,  C07K14/535 ,  C07K14/5412 ,  C07K14/5428 ,  C07K14/5446 ,  C07K14/70514 ,  C12N5/0637

摘要： Disclosed are methods of identifying immunosuppressive TR1 regulatory T cells, including in methods of diagnosing the presence of immune tolerance, methods of producing immunosuppressive regulatory T cells, and methods of eliciting immune tolerance in a subject. These methods include screening T cells to detect Eomes+IL-10+ T cells or expressing recombinant Eomes in T cell populations to generate immunosuppressive regulatory T cells.

公开(公告)号：US20230151438A1

公开(公告)日：2023-05-18

申请号：US17251393

申请日：2019-06-12

申请人： The Council of the Queensland Institute of Medical Research

发明人： Rajiv Khanna ,  Jacqueline Burrows

IPC分类号： C12Q1/70 ,  C12N5/0783 ,  C12N15/86

CPC分类号： C12Q1/701 ,  C12N5/0638 ,  C12N15/86 ,  C12N2710/10343 ,  C12N2710/16022 ,  C12N2502/11 ,  C12N2502/1121

摘要： Provided herein are methods for preparing and characterizing CTL cultures and preparations. Also provided herein are methods of determining the presence or absence of trace amounts of viral vectors following the preparation of CTLs.

公开(公告)号：US20220136061A1

公开(公告)日：2022-05-05

申请号：US17368449

申请日：2021-07-06

申请人： The Council of the Queensland Institute of Medical Research

发明人： Mitchell Stark

IPC分类号： C12Q1/6886 ,  C07K16/30 ,  C12Q1/6813

摘要： Provided are methods for determining or predicting the diagnosis, prognosis, treatment and therapeutic efficacy of melanoma in a subject, which include evaluating the expression level of one or more miRNA biomarkers including miRNA-4487, miRNA-4706, miRNA-4731 and fragments or variants thereof as an indication of whether the subject may have, or be predisposed to, melanoma.

公开(公告)号：US20220023419A1

公开(公告)日：2022-01-27

申请号：US17298312

申请日：2019-11-28

申请人： THE COUNCIL OF THE QUEENSLAND INSTITUTE OF MEDICAL RESEARCH

发明人： Geoffrey Hill ,  Motoko Koyama

IPC分类号： A61K39/395 ,  A61K38/14 ,  A61K38/12 ,  A61K31/7048 ,  A61K31/7036 ,  A61K31/546 ,  A61K35/741 ,  A61K35/24 ,  A61K38/08 ,  A61P37/06

摘要： The present disclosure relates to methods of reducing inflammation in the gastro-intestinal (GI) tract, and more specifically to methods of reducing the risk of transplant recipients developing graft-versus-host disease by reducing inflammation in the gut.

公开(公告)号：US11065329B2

公开(公告)日：2021-07-20

申请号：US15860145

申请日：2018-01-02

申请人： The Council of the Queensland Institute of Medical Research

发明人： Rajiv Khanna ,  Dasari Vijayendra

IPC分类号： A61K39/245 ,  C07K14/005 ,  C12N7/00 ,  C12N5/0783 ,  A61K39/00

摘要： An isolated protein comprises respective amino acid sequences of each of a plurality of CTL epitopes from two or more different herpesvirus antigens and further comprises an intervening amino acid or amino acid sequence between at least two of said CTL epitopes comprising proteasome liberation amino acids or amino acid sequences and, optionally, Transporter Associated with Antigen Processing recognition motifs. The isolated protein is capable of rapidly expanding human cytotoxic T lymphocytes (CTL) in vitro and eliciting a CTL immune response in vivo upon administration to an animal as an exogenous protein. Typically, the isolated protein comprises no more than twenty (20) CTL epitopes derived from cytomegalovirus and/or Epstein-Barr virus antigens.

公开(公告)号：US09901632B2

公开(公告)日：2018-02-27

申请号：US14436239

申请日：2013-10-21

申请人： The Council of the Queensland Institute of Medical Research

发明人： Rajiv Khanna ,  Dasari Vijayendra

IPC分类号： A61K39/245 ,  C12N7/00 ,  C07K14/005 ,  C12N5/0783 ,  A61K39/00

CPC分类号： A61K39/245 ,  A61K2039/572 ,  A61K2039/6031 ,  A61K2039/70 ,  C07K14/005 ,  C07K2319/00 ,  C07K2319/21 ,  C07K2319/40 ,  C12N5/0638 ,  C12N7/00 ,  C12N2501/998 ,  C12N2710/16122 ,  C12N2710/16134 ,  C12N2710/16222 ,  C12N2710/16234

摘要： An isolated protein comprises respective amino acid sequences of each of a plurality of CTL epitopes from two or more different herpesvirus antigens and further comprises an intervening amino acid or amino acid sequence between at least two of said CTL epitopes comprising proteasome liberation amino acids or amino acid sequences and, optionally, Transporter Associated with Antigen Processing recognition motifs. The isolated protein is capable of rapidly expanding human cytotoxic T lymphocytes (CTL) in vitro and eliciting a CTL immune response in vivo upon administration to an animal as an exogenous protein. Typically, the isolated protein comprises no more than twenty (20) CTL epitopes derived from cytomegalovirus and/or Epstein-Barr virus antigens.

公开(公告)号：US08367067B2

公开(公告)日：2013-02-05

申请号：US12488485

申请日：2009-06-19

申请人： David Jackson ,  Weiguang Zeng

发明人： David Jackson ,  Weiguang Zeng

IPC分类号： A61K39/00

CPC分类号： C07K14/775 ,  A61K38/00 ,  A61K39/0006 ,  A61K2039/543 ,  A61K2039/6018 ,  A61K2039/6068 ,  C07K19/00

摘要： The present invention provides synthetic immunogenic lipopeptide molecules comprising co-linear T-helper and B cell epitopes, and methods for their production and use in the generation of primary and secondary immune responses, and for the vaccination of animal subjects against particular antigens. More particularly, the present invention provides highly soluble lipopeptides wherein the lipid moiety is attached to the terminal side-chain group of an internal lysine or lysine analog, preferably to the terminal side-chain group of an internal diamino acid residue. Preferably the internal lysine or lysine analog is positioned between the T-helper epitope and the B cell epitope or within the T-helper epitope.

公开(公告)号：US20110280899A1

公开(公告)日：2011-11-17

申请号：US12901457

申请日：2010-10-08

申请人： David Jackson ,  Weiguang Zeng

发明人： David Jackson ,  Weiguang Zeng

IPC分类号： A61K39/145 ,  A61P37/04 ,  C07K7/08 ,  C07K14/00 ,  C07K7/06

CPC分类号： A61K39/39 ,  A61K39/07 ,  A61K39/12 ,  A61K39/145 ,  A61K39/29 ,  A61K39/385 ,  A61K2039/5158 ,  A61K2039/55516 ,  A61K2039/55544 ,  A61K2039/55566 ,  A61K2039/57 ,  A61K2039/6018 ,  A61K2039/6031 ,  A61K2039/6037 ,  A61K2039/6075 ,  C07K14/005 ,  C07K14/195 ,  C07K19/00 ,  C12N2760/16122 ,  C12N2760/16134 ,  C12N2760/18422 ,  C12N2770/24222 ,  C12N2770/24234

摘要： The present invention provides synthetic immunogenic lipopeptide molecules comprising co-linear T-helper and CTL epitopes, and methods for their production and use in the generation of primary and secondary immune responses, and for the vaccination of animal subjects against particular CTL epitopes. More particularly, the present invention provides highly soluble lipopeptides wherein the lipid moiety is attached to the terminal side-chain group of an internal lysine or lysine analog, preferably to the terminal side-chain group of an internal diamino acid residue. Preferably the internal lysine or lysine analog is positioned between the T-helper epitope and the CTL epitope.

摘要翻译： 本发明提供了包含共线T-辅助和CTL表位的合成的免疫原性脂肽分子，及其生产和用于产生初级和次级免疫应答的方法，以及针对特定CTL表位的动物受试者的疫苗接种。 更具体地，本发明提供高可溶性脂肽，其中脂质部分连接到内部赖氨酸或赖氨酸类似物的末端侧链基团，优选连接到内部二氨基酸残基的末端侧链基团。 优选地，内部赖氨酸或赖氨酸类似物位于T辅助表位和CTL表位之间。