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    Poxvirus expression system
    9.
    发明授权
    Poxvirus expression system 有权

    公开(公告)号：US09951352B2

    公开(公告)日：2018-04-24

    申请号：US15051410

    申请日：2016-02-23

    申请人： Isis Innovation Limited

    发明人： Matthew Guy Cottingham

    IPC分类号： C12N15/86 ,  A61K48/00 ,  C12N7/00 ,  C12N15/863 ,  C12N15/66 ,  A61K39/00

    CPC分类号： C12N15/86 ,  A61K39/00 ,  A61K48/00 ,  A61K2039/5256 ,  C12N7/00 ,  C12N15/66 ,  C12N15/8636 ,  C12N2710/24143 ,  C12N2800/204 ,  C12N2800/50 ,  C12N2830/34 ,  Y02A50/412

    摘要： There is provided a method for inserting a nucleic acid sequence that encodes a foreign peptide into a poxvirus genome, said method comprising: identifying in the poxvirus genome a poxvirus open reading frame wherein said open reading frame is characterized by an initial ATG start codon and wherein expression of said open reading frame is driven by an operably-linked poxvirus promoter located upstream of the open reading frame and wherein expression of said open reading frame provides a peptide that is non-essential to viability of the poxvirus; and inserting the nucleic acid sequence that encodes the foreign peptide at a position downstream of the poxvirus promoter; wherein following said insertion, (i) the nucleic acid that encodes the foreign peptide is operably-linked to the poxvirus promoter and expression of said nucleic acid is driven by said poxvirus promoter; and (ii) translation of the foreign peptide is initiated at an ATG start codon located at the same position as the ATG start codon of the poxvirus open reading frame. Also provided are a poxvirus vector and corresponding uses of the poxvirus vector in medicine.

    Poxvirus expression system
    10.
    发明授权
    Poxvirus expression system 有权
    痘病毒表达系统

    公开(公告)号：US09273327B2

    公开(公告)日：2016-03-01

    申请号：US13641512

    申请日：2011-04-15

    申请人： Matthew Guy Cottingham

    发明人： Matthew Guy Cottingham

    IPC分类号： C12N15/863 ,  C12N15/66 ,  A61K39/00 ,  C12N15/86

    CPC分类号： C12N15/86 ,  A61K39/00 ,  A61K48/00 ,  A61K2039/5256 ,  C12N7/00 ,  C12N15/66 ,  C12N15/8636 ,  C12N2710/24143 ,  C12N2800/204 ,  C12N2800/50 ,  C12N2830/34 ,  Y02A50/412

    摘要： There is provided a method for inserting a nucleic acid sequence that encodes a foreign peptide into a poxvirus genome, said method comprising: identifying in the poxvirus genome a poxvirus open reading frame wherein said open reading frame is characterized by an initial ATG start codon and wherein expression of said open reading frame is driven by an operably-linked poxvirus promoter located upstream of the open reading frame and wherein expression of said open reading frame provides a peptide that is non-essential to viability of the poxvirus; and inserting the nucleic acid sequence that encodes the foreign peptide at a position downstream of the poxvirus promoter; wherein following said insertion, (i) the nucleic acid that encodes the foreign peptide is operably-linked to the poxvirus promoter and expression of said nucleic acid is driven by said poxvirus promoter; and (ii) translation of the foreign peptide is initiated at an ATG start codon located at the same position as the ATG start codon of the poxvirus open reading frame. Also provided are a poxvirus vector and corresponding uses of the poxvirus vector in medicine.

    摘要翻译： 提供了将编码外源肽的核酸序列插入痘病毒基因组的方法，所述方法包括：在痘病毒基因组中鉴定痘病毒开放阅读框，其中所述开放阅读框的特征在于初始的ATG起始密码子，其中 所述开放阅读框的表达由位于开放阅读框上游的可操作连接的痘病毒启动子驱动，并且其中所述开放阅读框的表达提供了对痘病毒的活力非必需的肽; 并将编码外源肽的核酸序列插入到痘病毒启动子下游的位置; 其中在所述插入之后，（i）编码外来肽的核酸与痘病毒启动子可操作地连接，并且所述核酸的表达由所述痘病毒启动子驱动; 和（ii）外源肽的翻译起始于位于与痘病毒开放阅读框的ATG起始密码相同位置的ATG起始密码子。 还提供了痘病毒载体和痘病毒载体在药物中的相应用途。