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1.发明申请POLYPEPTIDE HAVING AFFINITY FOR ENVELOPE VIRUS CONSTITUENT AND USE THEREOF IN TRANSFERRING SUBSTANCE INTO CELL 有权具有包容性病毒组织的亲和力的多肽及其在细胞转运中的用途公开(公告)号：US20120301961A1
公开(公告)日：2012-11-29
申请号：US13546359
申请日：2012-07-11
申请人： Kuni FUSHIKIDA , Yoshitaka Kondo
发明人： Kuni FUSHIKIDA , Yoshitaka Kondo
IPC分类号： C12N5/071
CPC分类号： C07K14/005 , C07K2319/70 , C12N2760/18822 , C12N2760/18845 , C12N2810/6072
摘要： Delivery proteins are provided for transferring a protein, antibody or foreign substance into a cell without impairing the function or structure thereof. Further, methods of transferring a foreign substance into a cell at a high efficiency by using the delivery protein or an envelope virus or inactivated envelope virus in combination with said delivery protein are provided. The inventors discovered that a protein containing a polypeptide having an affinity for a constituent of the envelope virus contributes to the efficient enclosure of the foreign substance in the envelope. Moreover, the inventors discovered that use of the delivery protein enables foreign substances to be included in an envelope virus or inactivated envelope virus and therefore makes it possible to efficiently transfer the substances into cells without damaging the physiological function thereof.
摘要翻译： 提供递送蛋白质用于将蛋白质，抗体或异物转移到细胞中而不损害其功能或结构。 此外，提供了通过使用递送蛋白或包膜病毒或灭活的包膜病毒结合所述递送蛋白将异物高效转移到细胞中的方法。 本发明人发现含有对包膜病毒成分具有亲和性的多肽的蛋白质有助于将外源物质有效地包封在信封中。 此外，本发明人发现，使用递送蛋白质使得外来物质能够包含在包膜病毒或灭活的包膜病毒中，因此能够将物质有效地转移到细胞中而不损害其生理功能。
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2.发明授权Polypeptide having affinity for envelope virus constituent and use thereof in transferring substance into cell 有权对包膜病毒成分具有亲和性的多肽及其将物质转移至细胞中的用途公开(公告)号：US08388979B2
公开(公告)日：2013-03-05
申请号：US13546359
申请日：2012-07-11
申请人： Kuni Fushikida , Yoshitaka Kondo
发明人： Kuni Fushikida , Yoshitaka Kondo
IPC分类号： A61K39/00 , A61K39/12 , A61K39/385 , C12Q1/70 , G01N33/53 , G01N33/567 , C12P1/00
CPC分类号： C07K14/005 , C07K2319/70 , C12N2760/18822 , C12N2760/18845 , C12N2810/6072
摘要： Delivery proteins are provided for transferring a protein, antibody or foreign substance into a cell without impairing the function or structure thereof. Further, methods of transferring a foreign substance into a cell at a high efficiency by using the delivery protein or an envelope virus or inactivated envelope virus in combination with said delivery protein are provided. The inventors discovered that a protein containing a polypeptide having an affinity for a constituent of the envelope virus contributes to the efficient enclosure of the foreign substance in the envelope. Moreover, the inventors discovered that use of the delivery protein enables foreign substances to be included in an envelope virus or inactivated envelope virus and therefore makes it possible to efficiently transfer the substances into cells without damaging the physiological function thereof.
摘要翻译： 提供递送蛋白质用于将蛋白质，抗体或异物转移到细胞中而不损害其功能或结构。 此外，提供了通过使用递送蛋白或包膜病毒或灭活的包膜病毒结合所述递送蛋白将异物高效转移到细胞中的方法。 本发明人发现含有对包膜病毒成分具有亲和性的多肽的蛋白质有助于将外源物质有效地包封在信封中。 此外，本发明人发现，使用递送蛋白质使得外来物质能够包含在包膜病毒或灭活的包膜病毒中，因此能够将物质有效地转移到细胞中而不损害其生理功能。
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3.发明申请Polypeptide Having Affinity for Envelope Virus Constituent and Use Thereof in Transferring Substance Into Cell 审中-公开具有包膜病毒成分亲和力的多肽和将其转移到细胞中的用途公开(公告)号：US20090053790A1
公开(公告)日：2009-02-26
申请号：US12223464
申请日：2007-01-31
申请人： Kuni Fushikida , Yoshitaka Kondo
发明人： Kuni Fushikida , Yoshitaka Kondo
CPC分类号： C07K14/005 , C07K2319/70 , C12N2760/18822 , C12N2760/18845 , C12N2810/6072
摘要： Proteins are provided for transferring a protein, an antibody or another foreign substance into a cell without impairing the function or structure thereof; and methods of transferring a foreign substance into a cell in a time and quantity controllable manner at a high efficiency by using the above-described delivery protein or an envelope virus or inactivated envelope virus in combination with said delivery protein. As the results of intensive studies on a method of enclosing a foreign substance in the envelope of an envelope virus, it is found out that a protein containing a polypeptide having an affinity for a constituent of the envelope virus contributes to the efficient enclosure of the foreign substance in the envelope. Moreover, it is found out that use of the aforementioned protein enables foreign substances to be included in an envelope virus or inactivated envelope virus and therefore the resulting foreign substance-containing envelope virus or inactivated envelope virus makes it possible to efficiently transfer the substances into cells without damaging the physiological function thereof.
摘要翻译： 提供蛋白质用于将蛋白质，抗体或其他异物转移到细胞中而不损害其功能或结构; 以及通过使用上述递送蛋白或包膜病毒或灭活的包膜病毒与所述递送蛋白组合，以高效率以时间和数量可控的方式将异物转移到细胞中的方法。 作为将外来物质包封在信封病毒的包膜中的方法的深入研究的结果，发现含有对包膜病毒成分具有亲和性的多肽的蛋白质有助于外源的有效封闭 物质在信封。 此外，发现使用上述蛋白质可使外来物质被包含在信封病毒或灭活的包膜病毒中，因此产生的含异物的包膜病毒或灭活的包膜病毒能够有效地将物质转移到细胞中 而不损害其生理功能。
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公开(公告)号：US5265335A
公开(公告)日：1993-11-30
申请号：US984547
申请日：1992-12-02
CPC分类号： F16C23/041 , B23P15/003 , F16C33/14 , Y10S72/70 , Y10T29/49675 , Y10T29/49677
摘要： An inner sliding surface layer of a bearing bushing is formed into a crowning shape in an axial direction by a die shaping method. A bushing blank fitted on a generally-tapered lower end portion of an upper mandrel is inserted, together with the upper mandrel, into a hole in a forming die, and the bushing blank is deformed between the forming die, the upper mandrel and a lower mandrel having an upper end portion similar in shape to the lower end portion of the upper mandrel, thereby obtaining the bushing having the sliding inner peripheral surface of a crowning shape.
摘要翻译： 轴承衬套的内部滑动表面层通过模具成形方法沿轴向形成为凸面形状。 安装在上心轴的大致锥形下端部上的衬套坯料与上心轴一起插入成型模具中的孔中，衬套坯料在成型模具，上心轴和下心轴之间变形 心轴具有与上心轴的下端部相似形状的上端部，从而获得具有凸形形状的滑动内周面的衬套。
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公开(公告)号：US08691956B2
公开(公告)日：2014-04-08
申请号：US13498392
申请日：2010-09-29
申请人： Fuminori Kato , Yoshitaka Kondo
发明人： Fuminori Kato , Yoshitaka Kondo
CPC分类号： C07K16/18 , C07K2317/34 , C07K2317/92 , G01N33/6893 , G01N2333/4703 , G01N2800/125 , G01N2800/245 , G01N2800/32 , G01N2800/368 , G01N2800/50
摘要： This invention relates to a monoclonal antibody against a human HIG-1 polypeptide, the antibody binding to at least one epitope included in the amino acid sequence at positions 1-19 of a human HIG-1 polypeptide; an antibody fragment derived from the antibody; a DNA comprising a base sequence encoding a variable region of the antibody; an expression vector comprising the DNA; a cell line producing the antibody; a reagent for detecting a human HIG-1 polypeptide comprising the antibody or the antibody fragment, and a method for detecting a human HIG-1 polypeptide using the antibody or the antibody fragment.
摘要翻译： 本发明涉及针对人HIG-1多肽的单克隆抗体，所述抗体与人HIG-1多肽的位置1-19处的氨基酸序列中包含的至少一个表位结合; 衍生自抗体的抗体片段; 包含编码抗体可变区的碱基序列的DNA; 包含DNA的表达载体; 产生抗体的细胞系; 用于检测包含抗体或抗体片段的人HIG-1多肽的试剂，以及使用该抗体或抗体片段检测人HIG-1多肽的方法。
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公开(公告)号：US5274921A
公开(公告)日：1994-01-04
申请号：US984546
申请日：1992-12-02
IPC分类号： B21D37/10 , B21D53/10 , B21D53/16 , B32B15/04 , F16C17/02 , F16C23/04 , F16C33/06 , F16C33/14 , B21D19/00
CPC分类号： B21D53/16 , B21D53/10 , F16C17/02 , F16C23/04 , F16C33/14 , Y10S72/70 , Y10T29/49673 , Y10T29/49677
摘要： A bushing having an outer sliding surface is produced by a die forming method without machining. A crowning shape is provided to the outer surface so that the outer diameter of the bushing is smaller at its opposite longitudinal ends, and increases progressively toward a central zone of the bushing. A cylindrical bushing blank is fitted on distal end portion of a vertically-movable mandrel. Next, one of an upper die and a lower die is reactively moved toward the other to plastically work the bushing blank by the both dies and the mandrel, thereby working the bushing. An inner diameter of the upper die is larger at its lower end, and decreases progressively away from the lower end, and an inner diameter of the lower die is larger at its upper end, and decreases progressively away from the upper end.
摘要翻译： 具有外滑动面的衬套通过不进行机加工的模具成形方法制造。 顶部形状设置在外表面上，使得衬套的外径在其相对的纵向端部较小，并逐渐向套管的中心区域逐渐增加。 圆柱形衬套坯料安装在可垂直移动的心轴的远端部分上。 接下来，上模和下模中的一个被朝向另一个反应地移动以通过两个模具和心轴对衬套坯料进行塑性加工，从而对衬套进行加工。 上模的内径在其下端较大，并且从下端逐渐减小，下模的内径在其上端较大，并且从上端逐渐减小。
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公开(公告)号：US5433532A
公开(公告)日：1995-07-18
申请号：US290127
申请日：1994-08-15
CPC分类号： F16C33/08 , F16C33/105 , F16C33/1065 , Y10T29/49675
摘要： An annular bearing has its bearing surface on the inner surface. The bearing surface is formed to improve bearing properties, that is, to improve adaptability to an impact load and an imbalanced load. A method of manufacturing a bearing having such a bearing surface is given. An annular bearing blank, together with a mandrel having a plurality of arch-shaped ridges extending axially on the outer surface of the mandrel and a sleeve fitted on a periphery of the mandrel, is pushed into an inner hole of a molding die. The material is accordingly closed in a space defined by the mandrel, the inner hole, the sleeve and an ejector inserted into the bottom part of the inner hole. Thus, a plurality of arch-shaped recesses are formed extending axially on the inner surface of the bearing blank.
摘要翻译： 环形轴承在其内表面具有其支承表面。 形成轴承表面以改善轴承性能，即提高对冲击载荷和不平衡载荷的适应性。 给出了具有这种支承面的轴承的制造方法。 环形轴承坯料与具有在心轴的外表面上轴向延伸的多个拱形凸脊和安装在心轴的周边上的套筒的心轴一起被推入模具的内孔中。 因此，该材料在由心轴，内孔，套筒和插入内孔的底部的顶出件所限定的空间内封闭。 因此，在轴承坯件的内表面上轴向形成多个拱形凹部。
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公开(公告)号：US20120244560A1
公开(公告)日：2012-09-27
申请号：US13498392
申请日：2010-09-29
申请人： Fuminori Kato , Yoshitaka Kondo
发明人： Fuminori Kato , Yoshitaka Kondo
CPC分类号： C07K16/18 , C07K2317/34 , C07K2317/92 , G01N33/6893 , G01N2333/4703 , G01N2800/125 , G01N2800/245 , G01N2800/32 , G01N2800/368 , G01N2800/50
摘要： This invention relates to a monoclonal antibody against a human HIG-1 polypeptide, the antibody binding to at least one epitope included in the amino acid sequence at positions 1-19 of a human HIG-1 polypeptide; an antibody fragment derived from the antibody; a DNA comprising a base sequence encoding a variable region of the antibody; an expression vector comprising the DNA; a cell line producing the antibody; a reagent for detecting a human HIG-1 polypeptide comprising the antibody or the antibody fragment, and a method for detecting a human HIG-1 polypeptide using the antibody or the antibody fragment.
摘要翻译： 本发明涉及针对人HIG-1多肽的单克隆抗体，所述抗体与人HIG-1多肽的位置1-19处的氨基酸序列中包含的至少一个表位结合; 衍生自抗体的抗体片段; 包含编码抗体可变区的碱基序列的DNA; 包含DNA的表达载体; 产生抗体的细胞系; 用于检测包含抗体或抗体片段的人HIG-1多肽的试剂，以及使用该抗体或抗体片段检测人HIG-1多肽的方法。
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公开(公告)号：US5471862A
公开(公告)日：1995-12-05
申请号：US337141
申请日：1994-11-10
CPC分类号： F16C33/08 , F16C33/105 , F16C33/1065 , Y10T29/49675
摘要： An annular bearing has its bearing surface on the inner surface. The bearing surface is formed to improve bearing properties, that is, to improve adaptability to an impact load and an imbalanced load. A method of manufacturing a bearing having such a bearing surface is given. An annular bearing blank, together with a mandrel having a plurality of arch-shaped ridges extending axially on the outer surface of the mandrel and a sleeve fitted on a periphery of the mandrel, is pushed into an inner hole of a molding die. The material is accordingly closed in a space defined by the mandrel, the inner hole, the sleeve and an ejector inserted into the bottom part of the inner hole. Thus, a plurality of arch-shaped recesses are formed extending axially on the inner surface of the bearing blank.
摘要翻译： 环形轴承在其内表面具有其支承表面。 形成轴承表面以改善轴承性能，即提高对冲击载荷和不平衡载荷的适应性。 给出了具有这种支承面的轴承的制造方法。 环形轴承坯料与具有在心轴的外表面上轴向延伸的多个拱形凸脊和安装在心轴的周边上的套筒的心轴一起被推入模具的内孔中。 因此，该材料在由心轴，内孔，套筒和插入内孔的底部的顶出件所限定的空间内封闭。 因此，在轴承坯件的内表面上轴向形成多个拱形凹部。
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公开(公告)号：US4821402A
公开(公告)日：1989-04-18
申请号：US715947
申请日：1985-03-25
CPC分类号： B23Q3/101 , B23Q3/12 , B23Q7/046 , Y10T483/123 , Y10T483/171 , Y10T82/2516
摘要： An NC lathe with a robot for automatically exchanging works and chuck claws comprising a numerically controlled lathe having a rotatable chuck, a robot disposed in the vicinity of the numerically controlled lathe, and a feeder disposed in the vicinity of the robot. Groups of works and magazines containing chuck claws are placed on the pallets carried on the feeder, forming respective sets. The robot executes the exchanging operation of the works and the chuck claws between the feeder and the chuck.
摘要翻译： 一种具有用于自动交换作业的机器人和具有可旋转卡盘的数控车床的机器人的NC车床，设置在数控车床附近的机器人以及设置在机器人附近的进给器。 包含卡盘爪的作品和杂志组放置在馈送器上携带的托盘上，形成各个组。 机器人执行工件的交换操作和进给器和卡盘之间的卡盘爪。
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