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    Viral defective interfering particles and uses thereof
    4.
    发明授权
    Viral defective interfering particles and uses thereof 失效
    病毒性干扰颗粒及其用途

    公开(公告)号：US5980901A

    公开(公告)日：1999-11-09

    申请号：US933480

    申请日：1997-09-18

    申请人： Chiaho Shih ,  Ta-Tung Yuan

    发明人： Chiaho Shih ,  Ta-Tung Yuan

    IPC分类号： A61K35/76 ,  A61K39/00 ,  A61K39/29 ,  A61P31/12 ,  C07K14/02 ,  C12N7/00 ,  A61K39/12 ,  C12N7/04

    CPC分类号： C07K14/005 ,  A61K39/12 ,  A61K39/292 ,  C12N7/00 ,  A61K39/00 ,  C12N2730/10122 ,  C12N2730/10132 ,  C12N2730/10134 ,  Y10S977/802 ,  Y10S977/805 ,  Y10S977/915 ,  Y10S977/918

    摘要： The present invention provides a composition of matter comprising a defective interfering virus particle, wherein said particle naturally occurs in a human infection and wherein said particles has a naturally occurring core antigen internal deletion. Provided is a pharmaceutical composition, comprising defective interfering virus particle and a pharmaceutically acceptable carrier. Provided is a method for preparing defective interfering virus, comprising the steps of: (1) introduce a defective interfering virus and a complementing plasmid expressing a wild type virus core antigen and optionally containing a drug resistance gene, into a recipient cell; (2) selecting for stably transfected colonies; (3) growing the drug resistant cells and screening for the production of virus DNA replication; and (4) collecting defective interfering virus particles from the medium. Further provided is a vaccine, comprising a defective interfering virus particle.

    摘要翻译： 本发明提供了包含有缺陷的干扰性病毒颗粒的物质组合物，其中所述颗粒天然存在于人感染中，并且其中所述颗粒具有天然存在的核心抗原内部缺失。 提供了一种药物组合物，其包含有缺陷的干扰性病毒颗粒和药学上可接受的载体。 本发明提供一种制备有缺陷的干扰性病毒的方法，其特征在于包括以下步骤：（1）将受损细胞引入缺陷干扰病毒和表达野生型病毒核心抗原的互补质粒，并任选地含有耐药性基因; （2）选择稳定转染的菌落; （3）生长耐药细胞并筛选生产病毒DNA复制; 和（4）从介质中收集有缺陷的干扰性病毒颗粒。 还提供了包含有缺陷的干扰性病毒颗粒的疫苗。

    Peptides which enhance transport across tissues and methods of identifying and using the same
    6.
    发明申请
    Peptides which enhance transport across tissues and methods of identifying and using the same 失效
    增强跨组织转运的肽以及识别和使用它们的方法

    公开(公告)号：US20040023204A1

    公开(公告)日：2004-02-05

    申请号：US10104603

    申请日：2002-03-22

    发明人： Daniel Joseph O'Mahony ,  Vernon L. Alvarez ,  Michela Seveso

    IPC分类号： C12Q001/70 ,  C12Q001/68 ,  A61K049/00 ,  C12P019/34

    CPC分类号： C40B40/02 ,  A61K9/167 ,  A61K47/42 ,  A61K47/62 ,  A61K47/6937 ,  B82Y5/00 ,  C12N15/1037 ,  G01N33/68 ,  Y10S977/805

    摘要： A method of identifying a peptide which permits or facilitates the transport of an active agent through a human or animal tissue. A predetermined amount of phage from a random phage library or a preselected phage library is administered in vivo or in situ to a site in an animal, such as into the gastro-intestinal tract. At a predetermined time, the phage which is transported across a tissue barrier is harvested at a harvesting site, such as in portal or systemic blood or brain tissue, which is separated from the site of administration by the tissue barrier to select transported phage. This transported phage is amplified in a host. This cycle of events is repeated (using the transported phage produced in the most recent cycle) a predetermined number of times to obtain a selected phage library containing phage which can be transported from the site of administration to the harvesting site. The identity of at least one peptide coded by phage in the selected phage library is determined to identify a peptide which permits or facilitates the transport of an active agent through a human or animal tissue.

    摘要翻译： 鉴定允许或促进活性剂通过人或动物组织的运输的肽的方法。 来自随机噬菌体文库或预选噬菌体文库的预定量的噬菌体在体内或原位施用于动物如胃肠道中的部位。 在预定时间内，跨越组织屏障传送的噬菌体在收集部位收集，例如门静脉或全身血液或脑组织，其通过组织屏障从给药部位分离以选择输送的噬菌体。 这种传播的噬菌体在宿主中被扩增。 重复这个事件周期（使用最近一次循环中产生的运送的噬菌体）预定次数，以获得包含可从施用部位运送到收获部位的噬菌体的选定噬菌体文库。 测定所选择的噬菌体文库中由噬菌体编码的至少一种肽的身份，以鉴定允许或促进活性剂通过人或动物组织的转运的肽。

    Peptides which enhance transport across tissues and methods of identifying and using the same
    7.
    发明授权
    Peptides which enhance transport across tissues and methods of identifying and using the same 失效

    公开(公告)号：US07566766B2

    公开(公告)日：2009-07-28

    申请号：US10104603

    申请日：2002-03-22

    申请人： Daniel J. O'Mahony ,  Vernon L. Alvarez ,  Michela Seveso

    发明人： Daniel J. O'Mahony ,  Vernon L. Alvarez ,  Michela Seveso

    IPC分类号： C07K14/00

    CPC分类号： C40B40/02 ,  A61K9/167 ,  A61K47/42 ,  A61K47/62 ,  A61K47/6937 ,  B82Y5/00 ,  C12N15/1037 ,  G01N33/68 ,  Y10S977/805

    摘要： A method of identifying a peptide which permits or facilitates the transport of an active agent through a human or animal tissue. A predetermined amount of phage from a random phage library or a preselected phage library is administered in vivo or in situ to a site in an animal, such as into the gastro-intestinal tract. At a predetermined time, the phage which is transported across a tissue barrier is harvested at a harvesting site, such as in portal or systemic blood or brain tissue, which is separated from the site of administration by the tissue barrier to select transported phage. This transported phage is amplified in a host. This cycle of events is repeated (using the transported phage produced in the most recent cycle) a predetermined number of times to obtain a selected phage library containing phage which can be transported from the site of administration to the harvesting site. The identity of at least one peptide coded by phage in the selected phage library is determined to identify a peptide which permits or facilitates the transport of an active agent through a human or animal tissue.

    Compounds and methods for modulating desmosomal cadherin-mediated functions
    9.
    发明授权
    Compounds and methods for modulating desmosomal cadherin-mediated functions 失效
    调节体内钙粘蛋白介导功能的化合物和方法

    公开(公告)号：US06638911B1

    公开(公告)日：2003-10-28

    申请号：US09535852

    申请日：2000-03-27

    申请人： Orest W. Blaschuk ,  James Matthew Symonds ,  Barbara J. Gour

    发明人： Orest W. Blaschuk ,  James Matthew Symonds ,  Barbara J. Gour

    IPC分类号： A61K3808

    CPC分类号： C07K5/0808 ,  A61K38/00 ,  C07K5/1008 ,  C07K5/101 ,  C07K7/06 ,  C07K14/705 ,  C07K2319/00 ,  Y10S977/805

    摘要： Modulating agents for inhibiting or enhancing desmosomal cadherin mediated cell adhesion are provided. The modulating agents comprise one or more of: (a) a peptide sequence that is at least 50% identical to a desmosomal cadherin CAR sequence; (b) a non-peptide mimetic of a desmosomal cadherin CAR sequence; (c) a substance, such as an antibody or antigen-binding fragment thereof, that specifically binds a desmosomal cadherin CAR sequence; and/or (d) a polynucleotide encoding a polypeptide that comprises a desmosomal cadherin CAR sequence or analogue thereof. Methods for using such modulating agents for modulating desmosomal cadherin-mediated cell adhesion in a variety of contexts are also provided.

    摘要翻译： 提供了用于抑制或增强脱颗粒钙粘蛋白介导的细胞粘附的调节剂。 调节剂包含以下一种或多种：（a）与脱氨基体钙粘蛋白CAR序列至少50％相同的肽序列; （b）脱氨基体钙粘蛋白CAR序列的非肽模拟物; （c）特异性结合脱氨基体钙粘蛋白CAR序列的物质，如抗体或其抗原结合片段; 和/或（d）编码包含脱氨基体钙粘蛋白CAR序列或其类似物的多肽的多核苷酸。 还提供了在各种情况下使用这种调节剂调节溶酶体钙粘蛋白介导的细胞粘附的方法。

    Peptides which enhance transport across tissues and methods of identifying and using the same
    10.
    发明授权
    Peptides which enhance transport across tissues and methods of identifying and using the same 失效
    增强跨组织转运的肽以及识别和使用它们的方法

    公开(公告)号：US06361938B1

    公开(公告)日：2002-03-26

    申请号：US08857046

    申请日：1997-05-15

    申请人： Daniel Joseph O'Mahony ,  Vernon L. Alvarez ,  Michela Seveso

    发明人： Daniel Joseph O'Mahony ,  Vernon L. Alvarez ,  Michela Seveso

    IPC分类号： C12Q168

    CPC分类号： C40B40/02 ,  A61K9/167 ,  A61K47/42 ,  A61K47/62 ,  A61K47/6937 ,  B82Y5/00 ,  C12N15/1037 ,  G01N33/68 ,  Y10S977/805

    摘要： A method of identifying a peptide which permits or facilitates the transport of an active agent through a human or animal tissue. A predetermined amount of phage from a random phage library or a preselected phage library is administered in vivo or in situ to a site in an animal, such as into the gastro-intestinal tract. At a predetermined time, the phage which is transported across a tissue barrier is harvested at a harvesting site, such as in portal or systemic blood or brain tissue, which is separated from the site of administration by the tissue barrier to select transported phage. This transported phage is amplified in a host. This cycle of events is repeated (using the transported phage produced in the most recent cycle) a predetermined number of times to obtain a selected phage library containing phage which can be transported from the site of administration to the harvesting site. The identity of at least one peptide coded by phage in the selected phage library is determined to identify a peptide which permits or facilitates the transport of an active agent through a human or animal tissue.

    摘要翻译： 鉴定允许或促进活性剂通过人或动物组织的运输的肽的方法。 来自随机噬菌体文库或预选噬菌体文库的预定量的噬菌体在体内或原位施用于动物如胃肠道中的部位。 在预定时间内，跨越组织屏障传送的噬菌体在收集部位收集，例如门静脉或全身血液或脑组织，其通过组织屏障从给药部位分离以选择输送的噬菌体。 这种传播的噬菌体在宿主中被扩增。 重复这个事件周期（使用最近一次循环中产生的运送的噬菌体）预定次数，以获得包含可从施用部位运送到收获部位的噬菌体的选定噬菌体文库。 测定所选择的噬菌体文库中由噬菌体编码的至少一种肽的身份，以鉴定允许或促进活性剂通过人或动物组织的转运的肽。