公开(公告)号：US08652848B2

公开(公告)日：2014-02-18

申请号：US13488770

申请日：2012-06-05

Applicant: Takaaki Nagai ,  Hideaki Matsumoto ,  Yuichi Hamada

Inventor： Takaaki Nagai ,  Hideaki Matsumoto ,  Yuichi Hamada

IPC: G01N31/00 ,  C12Q1/68 ,  G01N33/00 ,  G01N15/06 ,  G06M11/02

CPC classification number: G01N15/12 ,  G01N1/38 ,  G01N15/1456 ,  G01N33/5094 ,  G01N33/80 ,  G01N33/86 ,  G01N35/00584 ,  G01N2015/0073 ,  G01N2015/0084 ,  G01N2015/0088 ,  Y10T436/101666

Abstract: A method for analyzing blood cells in a whole blood sample obtained from a cat is provided. An electrical measurement result and an optical measurement result of the whole blood sample are acquired. The electrical measurement result is obtainable by electrically measuring blood cells in the whole blood sample and the optical measurement result is obtainable by optically measuring blood cells in the whole blood sample. On the basis of the electrical measurement result and the optical measurement result, volume of red blood cells in the whole blood sample is calculated.

Abstract translation: 提供了一种用于分析从猫获得的全血样品中的血细胞的方法。 获得全血样品的电测量结果和光学测量结果。 通过电测量全血样品中的血细胞可以获得电测量结果，并且可以通过光学测量全血样品中的血细胞获得光学测量结果。 在电气测量结果和光学测量结果的基础上，计算出全血样品中红细胞的体积。

公开(公告)号：US20070159627A1

公开(公告)日：2007-07-12

申请号：US11328033

申请日：2006-01-09

Applicant: Paul Johnson

Inventor： Paul Johnson

IPC: G06M11/02 ,  G01N15/02

CPC classification number: G01N15/1463 ,  G01N15/1459 ,  G01N2015/1486

Abstract: An invention is described which allows measurement of the concentration of fluorescent particles in a flowing (laminar) fluid by imaging the flow with a video camera. A beam of illumination is used to illuminate the target particles. Imaging optics are arranged to view the focal plane to form an image of the multiple fluorescent sample particles in the flow stream; a camera records the image formed by the imaging optics, and a counting algorithm enumerates the particles. Operational parameters of the system are adjusted according to an initial estimate of particle density, for example flow rate, exposure time, and sampling interval. In addition, the counting algorithm is selected according to the estimated particle density.

Abstract translation: 描述了允许通过用摄像机对流动进行成像来测量流动（层流）流体中的荧光颗粒的浓度的发明。 照射光束用于照射目标颗粒。 成像光学器件布置成观察焦平面以在流动流中形成多个荧光样品颗粒的图像; 相机记录由成像光学元件形成的图像，并且计数算法列举了粒子。 根据粒子密度的初始估计，例如流速，曝光时间和采样间隔来调整系统的操作参数。 另外，根据估计的粒子密度来选择计数算法。

公开(公告)号：US5978435A

公开(公告)日：1999-11-02

申请号：US983043

申请日：1998-01-15

Applicant: Steen Kold Christensen ,  Christian Born ,  Tove Asmussen ,  Mogens Bering Larsen

Inventor： Steen Kold Christensen ,  Christian Born ,  Tove Asmussen ,  Mogens Bering Larsen

IPC: G01N15/14 ,  G06M11/00 ,  G06M11/02

CPC classification number: G01N15/1456 ,  G06M11/00 ,  G01N2015/1486

Abstract: A method and apparatus for determining the number of particles or cells in a liquid sample. The method comprises a) determining the number of particles or cells in a first volume of the liquid sample, b) determining the statistical uncertainty of the determined number of particles in said first volume, c) if the determined uncertainty is larger than a pre-determined value, determining the number of particles or cells in a further volume of the liquid sample, d) adding the numbers of cells or particles determined in steps a) and c), e) determining the statistical uncertainty of the number of particles determined in step d), f) repeating steps d) and e) with a further volumes of the liquid sample, until the uncertainty determined in step e) is lower than said pre-determined value or until a pre-determined total volume of the liquid sample in which the number of cells or particles has been determined exceeds a pre-determined volume being larger than said first volume. In this way, the volume examined and the uncertainty of the measurement may be selected so that a suitable compromise between the uncertainty and the time required to perform the measurement can be obtained. The apparatus according to the invention comprises a liquid flow system allowing the measurement sequence to be interrupted as soon as the calculating means decide that the uncertainty of the actual cell count is small enough to rely on the present determination. The apparatus will then be ready to carry on with the next sample, thereby maintaining a high capacity while obtaining reliable cell counts.

Abstract translation: PCT No.PCT / DK96 / 00351 Sec。 371日期1998年1月15日 102（e）1998年1月15日PCT PCT 1996年8月20日PCT公布。 公开号WO97 / 07390 日期1997年2月27日用于确定液体样品中的颗粒或细胞数量的方法和装置。 该方法包括a）确定液体样品的第一体积中的颗粒或细胞的数量，b）确定所述第一体积中所确定的颗粒数量的统计学不确定度，c）如果所确定的不确定度大于预先确定的， 确定的值，确定液体样品的另一体积中的颗粒或细胞的数量，d）添加在步骤a）和c）中确定的细胞或颗粒的数量，e）确定测定的颗粒数量的统计学不确定性 步骤d），f）用另外体积的液体样品重复步骤d）和e），直到步骤e）中确定的不确定度低于所述预定值或直到液体样品的预定总体积 其中已经确定细胞或颗粒的数量超过比所述第一体积大的预定体积。 以这种方式，可以选择检查的体积和测量的不确定性，使得可以获得不确定性和执行测量所需的时间之间的适当折中。 根据本发明的装置包括一个液体流动系统，一旦计算装置决定实际细胞计数的不确定性足够小以依赖于当前的确定，允许测量序列被中断。 然后该设备将准备好进行下一个样品，从而在获得可靠的细胞计数的同时保持高容量。

公开(公告)号：US5793833A

公开(公告)日：1998-08-11

申请号：US665809

申请日：1996-06-19

Applicant: Toshihiko Imai

Inventor： Toshihiko Imai

IPC: G01N15/00 ,  G01N15/10 ,  G06M11/02

CPC classification number: G01N15/10 ,  G01N2015/0053

Abstract: To evaluate cleanliness of wafer accommodating members used for storing or transporting of silicon, gallium arsenide or like semiconductor wafers, pure water is poured into a vessel-like wafer accommodating member, such as a wafer case body, a top cover, etc., capable of containing liquid therein, and then low frequency vibrations or supersonic wave is applied to the member. Alternatively, inner wafer accommodating members accommodated in the wafer case body, such as a wafer basket, a wafer retainer, etc., are accommodated in a vessel containing poured pure water, and the low frequency vibrations or supersonic wave is applied to the members. Then, the quantity of particles in the water is counted. Particles that have been attached to the wafer case or to inner wafer accommodating members accommodated therein, such as a wafer basket, a wafer retainer, etc., are made readily separable into the water, thus permitting quantitative detection of generation of particles from the inner wafer accommodating members.

Abstract translation: 为了评估用于存储或输送硅，砷化镓等半导体晶片的晶片容纳构件的清洁度，将纯水倒入容器状晶片容纳构件中，例如晶片壳体，顶盖等，其能够 在其中容纳液体，然后将低频振动或超声波施加到构件。 或者，容纳在诸如晶片篮，晶片保持器等的晶片壳体中的内晶片容纳构件容纳在容纳倾倒的纯水的容器中，并且将低频振动或超声波施加到构件。 然后，计算水中的颗粒数量。 已经附着到晶片盒或容纳在其中的内晶片容纳元件（例如晶片篮，晶片保持器等）的颗粒容易地分离到水中，从而允许定量检测从内部产生的颗粒 晶片容纳部件。

公开(公告)号：US4015108A

公开(公告)日：1977-03-29

申请号：US636273

申请日：1975-11-28

Applicant: Roger Roy Adams Morton

Inventor： Roger Roy Adams Morton

IPC: G06K9/46 ,  G06T7/60 ,  G06M11/02

CPC classification number: G06T7/60 ,  G06K9/4638

Abstract: Apparatus for determining parameters of any number of selected regularly or randomly oriented particles, or images or image patterns thereof, of various size and shape lying within a discrete region. A scanning device is swept over the discrete region causing the generation of electrical signals in response to the interception of the boundaries of one or more of the particles. Apparatus, including, for example, a light pen, for use in cooperation with a display of the feature images is operated to best identify, select or prepare for analysis features of interest within the discrete region. In cooperation with such apparatus appropriate logic and electronic circuitry which is adapted to receive the initially generated signals provide, in response thereto, other signals related to the feature or features of interest and any particular parameter related to those features.

Abstract translation: 用于确定位于离散区域内的各种尺寸和形状的任何数量的所选定期或随机取向的颗粒或其图像或图像图案的参数的装置。 扫描装置扫过离散区域，导致响应于截取一个或多个颗粒的边界而产生电信号。 操作包括例如用于与特征图像的显示器配合使用的光笔的装置，以最佳地识别，选择或准备在离散区域内的感兴趣的分析特征。 与这种装置协作，适于接收最初生成的信号的适当的逻辑电路和电子电路响应于此提供与感兴趣的特征或特征有关的其它信号以及与这些特征有关的任何特定参数。

公开(公告)号：US3922532A

公开(公告)日：1975-11-25

申请号：US55848675

申请日：1975-03-14

Applicant: ARTEK SYST CORP

Inventor： KITCHENER WILLIAM R ,  SAMA STEPHEN L ,  TOLLES WALTER E

IPC: G06M11/02 ,  H04N7/18

CPC classification number: G06M11/02

Abstract: An automatic cell counter which includes a video camera for viewing a sample being scanned. The video output is quantized by means of a level detector which compensates for variations and non-uniformities of the background field and produces a signal identifying a cell as having a certain absorption relative to a preselected absorption level. The quantized signal from the level detector passes through a discriminator which produces an output flag for each cell having a predetermined size level. Thus, cells can be selected having a combination of predetermined size and absorption levels. By utilizing line to line comparison, a single flag is provided for each cell desired to be counted. The flags are counted and the entire video output is displayed with the flag superimposed upon each cell counted.

Abstract translation: 一种自动细胞计数器，其包括用于观察被扫描的样品的摄像机。 视频输出通过电平检测器进行量化，电平检测器补偿背景场的变化和非均匀性，并产生识别细胞相对于预选吸收水平具有一定吸收的信号。 来自电平检测器的量化信号通过鉴别器，其产生具有预定尺寸电平的每个单元的输出标志。 因此，可以选择具有预定尺寸和吸收水平的组合的细胞。 通过利用线对线比较，为希望被计数的每个单元提供单个标志。 对这些标记进行计数，并且显示整个视频输出，并且标记叠加在每个单元格上。

公开(公告)号：US3811036A

公开(公告)日：1974-05-14

申请号：US29040372

申请日：1972-09-19

Applicant: ARTEK SYST CORP

Inventor： PERRY R

IPC: G06M11/00 ,  C12M1/34 ,  G06M11/02

CPC classification number: C12M41/36 ,  G06M11/02

Abstract: An automatic colony counter which includes a television camera for viewing a sample being scanned. The output from the television camera is sent through control circuitry to a television monitor. The video signal is also processed and quantized to produce a digital count representing the number of colonies counted and also to produce a flag signal which causes an illuminated dot to be superimposed on each colony counted, thereby insuring that all the colonies in the sample have been counted.

Abstract translation: 一种自动菌落计数器，其包括用于观察被扫描的样品的电视摄像机。 电视摄像机的输出通过控制电路发送到电视监视器。 视频信号也被处理和量化以产生代表所计数的菌落数的数字计数，并且还产生一个标志信号，该标志信号使得每个殖民地上的点被叠加，从而确保样品中的所有菌落已被 算了

公开(公告)号：US3742194A

公开(公告)日：1973-06-26

申请号：US3742194D

申请日：1971-05-03

Applicant: TECHNICAN INSTR CORP

Inventor： CARUSO S ,  ISENBERG H ,  REICHLER A ,  WISEMAN D

IPC: G06F19/00 ,  G06M11/02

CPC classification number: G06M11/02

Abstract: A method and apparatus is described for determining the susceptibility of a significant microorganism in a biological sample to different anti-microbial agents, and for providing its antibiotic susceptibility profile. Quotients of the biological sample are provided to a plurality of biological chambers, two of such chambers being used as internal ''''start'''' and ''''reference'''' controls. In the remaining biological chambers, proliferation of the significant microorganism is challenged by the addition of selected antibiotics. In the ''''start'''' control chamber the microorganisms are ''''killed'''' simultaneously with the addition of antibiotics to the biological chambers; after incubation, the microorganisms in the remaining biological chambers and, also, the ''''reference'''' control chambers are ''''killed'''' concurrently. Each of the biological chambers and the control chambers are sampled, and the total particulates, i.e., nonviable and ''''killed'''' microorganisms and, also, any dust or dirt particles, are counted in particular sequence, and the respective counts are logically related, so as to signify the susceptibility of the significant microorganism to each of the antibiotics. The results are graphically recorded, so as to provide an antibiotic susceptibility profile of the significant organisms.

Abstract translation: 描述了用于确定生物样品中的重要微生物对不同抗微生物剂的易感性以及用于提供其抗生素敏感性特征的方法和装置。 将生物样品的商品提供给多个生物室，其中两个用作内部“开始”和“参考”对照。 在剩余的生物室中，通过添加选择的抗生素来挑战重要微生物的增殖。 在“启动”控制室中，微生物与生物室中加入抗生素同时“杀死”; 孵化后，剩余生物室中的微生物以及“参考”对照室同时被“杀死”。 对每个生物室和控制室进行采样，总的颗粒物，即不可维护的和“杀死的”微生物以及任何灰尘或污垢颗粒以特定顺序进行计数，并且相应的计数在逻辑上相关，因此 表示显着微生物对每种抗生素的敏感性。 结果以图形方式记录，以提供重要生物体的抗生素敏感性。

公开(公告)号：US3736432A

公开(公告)日：1973-05-29

申请号：US3736432D

申请日：1971-03-22

Applicant: VARIAN ASSOCIATES

Inventor： SWEET R

IPC: C12M1/34 ,  G06M11/02 ,  G06K7/00

CPC classification number: C12M41/36 ,  G06M11/02 ,  Y10S435/808

Abstract: Light from a localized source is projected through a transparent bacterial culture medium having a multitude of bacterial colonies formed on the surface thereof. The bacterial colonies serve as lenses for focusing images of the light source into a pattern of images which are projected into an image plane forming a pattern of light images corresponding to the pattern of bacterial colonies. A linear array of photodetectors is disposed in the projected image plane. The optical system is scanned relative to the bacterial colony culture such that adjacent scanning tracks overlap each other by approximately the track width of two photodetectors of the array of photodetectors. An output pulse, which is to be counted, from one detector inhibits counting output pulses from its nearest neighbors and the output pulses from the detectors at opposite ends of the array are not counted to form guard detectors, whereby colonies are accurately counted and colonies which overlap adjacent scanning tracks are counted but only once.

Abstract translation: 来自局部源的光通过在其表面上形成有多个细菌菌落的透明细菌培养基投影。 细菌菌落用作用于将光源的图像聚焦成图像的图案的图像，图像的图案被投影到形成与细菌菌落图案相对应的光图像的图案的图像平面中。 在投影图像平面中设置光电检测器的线性阵列。 相对于细菌菌落培养物扫描光学系统，使得相邻的扫描轨道彼此重叠大约光电检测器阵列的两个光电检测器的轨道宽度。 要从一个检测器计数的输出脉冲禁止从其最近的邻居计数输出脉冲，并且不计数来自阵列的相对端处的检测器的输出脉冲，以形成保护检测器，由此可以精确地计数菌落和菌落， 重叠相邻的扫描轨道被计数但只有一次。

公开(公告)号：US20180364145A1

公开(公告)日：2018-12-20

申请号：US16015807

申请日：2018-06-22

Applicant: Becton, Dickinson and Company

Inventor： Collin A. Rich

IPC: G01N15/14 ,  G06F19/26 ,  G06F19/12 ,  G06F19/10 ,  G01J3/02 ,  G01J3/36 ,  G01J3/18 ,  G06M11/02

CPC classification number: G01N15/1434 ,  G01J3/0208 ,  G01J3/0229 ,  G01J3/18 ,  G01J3/2803 ,  G01J3/36 ,  G01N15/14 ,  G01N15/1404 ,  G01N15/1429 ,  G06M11/02 ,  G16B5/00 ,  G16B45/00 ,  G16B99/00

Abstract: Systems in a flow cytometer having an interrogation zone and illumination impinging the interrogation zone include: a lens subsystem including a collimating element that collimates light from the interrogation zone, a light dispersion element that disperses collimated light into a light spectrum, and a focusing lens that focuses the light spectrum onto an array of adjacent detection points; a detector array, including semiconductor detector devices, that collectively detects a full spectral range of input light signals, in which each detector device detects a subset spectral range of the full spectral range of light signals; and a user interface that enables a user to create a set of virtual detector channels by grouping detectors in the detector array, such that each virtual detector channel corresponds to a detector group and has a virtual detector channel range including the sum of subset spectral ranges of the detectors in the corresponding detector group.