公开(公告)号：WO2005089945A1

公开(公告)日：2005-09-29

申请号：PCT/US2005/008248

申请日：2005-03-11

Applicant: BIOTROVE, INC. ,  KANIGAN, Tanya, S. ,  SMITH, Steve ,  LINTON, John ,  HESS, Robert ,  YODER, Karl ,  BRENNAN, Colin

Inventor： KANIGAN, Tanya, S. ,  SMITH, Steve ,  LINTON, John ,  HESS, Robert ,  YODER, Karl ,  BRENNAN, Colin

IPC: B01L3/00

CPC classification number: B01L3/5085 ,  B01L3/0241 ,  B01L3/0244 ,  B01L3/5025 ,  B01L3/50857 ,  B01L3/563 ,  B01L2200/021 ,  B01L2200/027 ,  B01L2200/0605 ,  B01L2200/0636 ,  B01L2200/0642 ,  B01L2200/16 ,  B01L2300/0816 ,  B01L2300/0829 ,  B01L2300/0864 ,  B01L2300/12 ,  B01L2300/161 ,  B33Y70/00 ,  B33Y80/00 ,  Y10T137/0318 ,  Y10T137/1624 ,  Y10T137/206 ,  Y10T436/2525

Abstract: An interface is provided for storing microfluidic samples in a nanoliter sample chip (10). A fluid access structure provides a fluid access region to a selected subset (20) of sample wells (12) from an array of sample wells (12). A fluid introduction mechanism introduces a sample fluid to the fluid access region so that the sample wells (12) in the selected subset (20) are populated with the sample fluid without the unselected sample wells being populated with the sample fluid.

Abstract translation: 提供了用于将微流体样品存储在纳升级样品芯片（10）中的界面。 流体通道结构从样品阱阵列（12）向样品阱（12）的选定子集（20）提供流体通路区域。 流体引入机构将样品流体引入流体存取区域，使得所选择的子集（20）中的样品阱（12）填充有样品流体，而未选择的样品孔填充有样品流体。

公开(公告)号：WO2005028629A2

公开(公告)日：2005-03-31

申请号：PCT/US2004/030520

申请日：2004-09-17

Applicant: APPLERA CORPORATION ,  HARDING, Ian ,  SANDELL, Don ,  BODNER, Kevin ,  PATEL, Jaynish ,  WOUDENBERG, Timothy ,  OLDHAM, Mark ,  KAO, H. Pin ,  LIM, Gary ,  BODEAU, John ,  BAHATT, Dar ,  ERMAKOV, Sergey ,  FAWCETT, Adrian

Inventor： HARDING, Ian ,  SANDELL, Don ,  BODNER, Kevin ,  PATEL, Jaynish ,  WOUDENBERG, Timothy ,  OLDHAM, Mark ,  KAO, H. Pin ,  LIM, Gary ,  BODEAU, John ,  BAHATT, Dar ,  ERMAKOV, Sergey ,  FAWCETT, Adrian

IPC: C12N

CPC classification number: B01L3/50851 ,  B01L3/50853 ,  B01L3/50857 ,  B01L2200/0642 ,  B01L2300/044 ,  B01L2300/0819 ,  B01L2300/0829 ,  B01L2400/0409 ,  B01L2400/0487 ,  C12Q1/6837

Abstract: The present invention provides methods for simultaneously determining the genetic expression profile an individual member of a species relative to a standard genome for said species, comprising distributing a sample comprising substantially all the genetic material of said member into an array of reaction chambers on a substrate, wherein each chamber has a volume of less than about 1 microliter, and each chamber comprises (1) a primer for a polynucleotide target within said standard genome, and (2) a probe associated with said primer which emits a concentration dependent signal if the primer binds with said target, and the array comprises at least one chamber comprising a primer for each of the polynucleotide target within said standard genome; performing an amplification reaction on the distributed sample in the array so as to increase the concentration of polynucleotides in each of the chambers in which the polynucleotide binds to a primer; and identifying which of the reaction chambers contains a polynucleotide that has been bound to a primer, by detecting the presence of the probe associated with the primer. In one embodiment, the organism is human, and the array comprises primers for 30,000 genomic polynucleotides. In one embodiment, the amplification is PCR.

Abstract translation: 本发明提供了用于同时确定物种的单个成员相对于所述物种的标准基因组的遗传表达谱的方法，包括将包含所述成员的基本上所有遗传物质的样品分布在基底上的反应室阵列中， 其中每个室具有小于约1微升的体积，并且每个室包含（1）用于所述标准基因组内的多核苷酸靶的引物，和（2）与所述引物相关的探针，如果引物 与所述靶结合，并且所述阵列包含至少一个室，其包含用于所述标准基因组内的多核苷酸靶的每一个的引物; 对阵列中的分布样品进行扩增反应，以增加其中多核苷酸与引物结合的每个室中的多核苷酸的浓度; 并且通过检测与引物相关的探针的存在，鉴定哪个反应室含有已结合引物的多核苷酸。 在一个实施方案中，生物是人，并且阵列包含30,000个基因组多核苷酸的引物。 在一个实施方案中，扩增是PCR。

公开(公告)号：WO2004089532A1

公开(公告)日：2004-10-21

申请号：PCT/EP2004/003720

申请日：2004-04-07

Applicant: PLACHOV, Dimitrij ,  GAVRUSHKIN, Alexander

Inventor： PLACHOV, Dimitrij ,  GAVRUSHKIN, Alexander

IPC: B01J19/00

CPC classification number: B01L3/50857 ,  B01J19/0046 ,  B01J2219/00319 ,  B01J2219/00427 ,  B01J2219/0043 ,  B01J2219/00475 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00641 ,  B01J2219/00668 ,  B01J2219/00673 ,  B01J2219/00707 ,  B01J2219/0072 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01L3/5025 ,  B01L2300/069 ,  B01L2300/0819 ,  C40B40/06 ,  C40B40/10 ,  C40B60/14

Abstract: Chip zur Untersuchung biologischen Materials mit einer Trägereinheit aus porösem Material, an dem die der Detektion der Probenmoleküle dienenden Referenzmoleküle immobilisiert sind.

Abstract translation: 芯片用于检查的生物材料与至所述检测样品分子的的服务基准分子多孔材料制成的载体单元被固定。

公开(公告)号：WO2004005924A1

公开(公告)日：2004-01-15

申请号：PCT/IB2002/003248

申请日：2002-07-03

Applicant: SPINELIX ,  CARIA, Mario ,  MAZZA, Valérie ,  MELIS, Cinzia ,  SESSELEGO, Alessio

Inventor： CARIA, Mario ,  MAZZA, Valérie ,  MELIS, Cinzia ,  SESSELEGO, Alessio

IPC: G01N33/543

CPC classification number: G01N33/54366 ,  B01J2219/00313 ,  B01J2219/00533 ,  B01J2219/00572 ,  B01J2219/00644 ,  B01J2219/00659 ,  B01J2219/00702 ,  B01J2219/00725 ,  B01L3/502 ,  B01L3/508 ,  B01L3/50857 ,  B01L2300/0636 ,  B01L2400/0415 ,  C40B40/10 ,  C40B60/14 ,  C40B70/00

Abstract: The invention relates to a new device and method for analyzing the interaction between reagent(s) and solutes or components, wherein said solutes or components diffuse across a matrix immobilizing said reagent(s), said matrix being located in a through hole within said device.

Abstract translation: 本发明涉及一种用于分析试剂和溶质或组分之间的相互作用的新装置和方法，其中所述溶质或组分扩散穿过固定所述试剂的基质，所述基质位于所述装置内的通孔中 。

公开(公告)号：WO0242775A3

公开(公告)日：2003-12-31

申请号：PCT/US0144868

申请日：2001-11-27

Applicant: GENEVENTION L L C ,  JACOBS ALICE ANNE ,  NIKOLIC BORIS ,  GUPTA VINEET

Inventor： JACOBS ALICE ANNE ,  NIKOLIC BORIS ,  GUPTA VINEET

IPC: B01F13/00 ,  B01F13/08 ,  B01J19/00 ,  B01L3/00 ,  B81B1/00 ,  C12Q1/68 ,  C40B40/06 ,  C40B40/10 ,  C40B40/12 ,  C40B40/18 ,  C40B60/14 ,  C40B70/00 ,  G01N33/15 ,  G01N33/50 ,  G01N33/53 ,  G01N33/543 ,  G01N33/545 ,  G01N33/547 ,  G01N33/551 ,  G01N33/552 ,  G01N33/553 ,  G01N37/00 ,  G01N33/569

CPC classification number: B01F13/0088 ,  B01F13/08 ,  B01F13/0809 ,  B01J19/0046 ,  B01J2219/00315 ,  B01J2219/00317 ,  B01J2219/00481 ,  B01J2219/00497 ,  B01J2219/00509 ,  B01J2219/00511 ,  B01J2219/00527 ,  B01J2219/00547 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00662 ,  B01J2219/00664 ,  B01J2219/00677 ,  B01J2219/00689 ,  B01J2219/00702 ,  B01J2219/0072 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00731 ,  B01J2219/0074 ,  B01J2219/00745 ,  B01L3/5027 ,  B01L3/50273 ,  B01L3/5085 ,  B01L3/50857 ,  B01L3/563 ,  B01L2300/021 ,  B01L2300/0636 ,  B01L2300/069 ,  B01L2300/0819 ,  B01L2300/0864 ,  B82Y30/00 ,  C12Q1/6837 ,  C40B40/06 ,  C40B40/10 ,  C40B40/12 ,  C40B40/18 ,  C40B60/14 ,  C40B70/00 ,  G01N33/53 ,  G01N33/543 ,  G01N33/54366

Abstract: The invention relates to the clinically intelligent design of diagnostic devices (such as microarrays) and methods of making and using such devices in differential diagnoses of specific clinical symptoms or sets of symptoms. In one aspect, the devices include various probes used to perform parallel screening of a number of analytes. The probes are clustered on the devices based on known clinical presentations of symptoms associated with specific diseases and disorders.

Abstract translation: 本发明涉及诊断装置（例如微阵列）的临床智能设计以及制造和使用这些装置在特定临床症状或症状组的鉴别诊断中的方法。 在一个方面，所述装置包括用于执行多个分析物的并行筛选的各种探针。 基于与特定疾病和病症相关的症状的已知临床表现，将探针聚集在装置上。

公开(公告)号：WO2003055589A2

公开(公告)日：2003-07-10

申请号：PCT/EP2002/014763

申请日：2002-12-27

Applicant: INSTITUT FÜR PHYSIKALISCHE HOCHTECHNOLOGIE E.V. ,  ALBERT, Jens ,  MAYER, Günter ,  SCHOBER, Andreas ,  GROSS, Alexander

Inventor： ALBERT, Jens ,  MAYER, Günter ,  SCHOBER, Andreas ,  GROSS, Alexander

IPC: B01J19/00

CPC classification number: B01L3/50853 ,  B01J2219/00286 ,  B01J2219/00315 ,  B01J2219/00333 ,  B01J2219/00423 ,  B01J2219/005 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01L3/5025 ,  B01L3/50851 ,  B01L3/50857 ,  B01L2300/0681 ,  C40B60/14

Abstract: Eine im Nanobereich verwendbare Mikrotiterplatte zur gleichzeitigen Durchführung vieler Mikrosynthesen unter erhöhten Temperaturen ist so zu gestalten, dass die einzelnen Mikrokammern der Mikro- bzw. Nanokammermatrix dicht verschließbar sind und das Verschließen vor bzw. das Öffnen der Mikrokammern nach einer Reaktion automatisierbar ist. Die eingefüllten Substanzen befinden sich in oben und unten offenen Kammern, die durch Zwischenwände voneinander getrennt und durch Abdeckungen verschließbar sind. Die Platte ist zumindest im Kammerbereich mehrlagig und dicht gestaltet und die Dicke der Zwischenwände an den Kammeröffnungen ist geringer als im Inneren der Kammern. Zwischen den Bereichen unterschiedlicher Dicke der Zwischenwände bestehen vorspringende Kanten.

Abstract translation: 对于在升高的温度同时进行许多微合成的有用的纳米级微量滴定板是被设计为使得微或纳米室矩阵的单独的微区室是紧密封闭和之前的关闭和的微室的开口可以是自动化进行反应。 填充物质位于顶部和底部的开口腔室，其通过分隔壁由盖彼此分开和可闭合。 将板以多层配置并至少在所述腔室区域密封，中间壁与所述腔室端口的厚度小于在所述腔室的内部。 由突出不同厚度的中间壁的区域之间的边缘。

公开(公告)号：WO2002078834A2

公开(公告)日：2002-10-10

申请号：PCT/US2002/005316

申请日：2002-02-19

Applicant: GENOSPECTRA, INC. ,  XIAO, Jianming ,  CHEN, Shiping ,  LUO, Yuling ,  BUTLER, John ,  QAZEN, Masoud ,  THIELE, Jochen ,  CHEN, Anthony

Inventor： XIAO, Jianming ,  CHEN, Shiping ,  LUO, Yuling ,  BUTLER, John ,  QAZEN, Masoud ,  THIELE, Jochen ,  CHEN, Anthony

IPC: B01J16/00

CPC classification number: G01N21/6452 ,  B01J19/0046 ,  B01J2219/00315 ,  B01J2219/00369 ,  B01J2219/00382 ,  B01J2219/00385 ,  B01J2219/00387 ,  B01J2219/00418 ,  B01J2219/00454 ,  B01J2219/0047 ,  B01J2219/00504 ,  B01J2219/00511 ,  B01J2219/00527 ,  B01J2219/00529 ,  B01J2219/00585 ,  B01J2219/00587 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/00612 ,  B01J2219/00619 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00702 ,  B01J2219/00704 ,  B01J2219/00707 ,  B01J2219/0072 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00731 ,  B01L3/0241 ,  B01L3/0244 ,  B01L3/5025 ,  B01L3/50857 ,  B01L9/523 ,  B01L9/54 ,  B01L2200/025 ,  B01L2200/0642 ,  B01L2300/0609 ,  B01L2300/0645 ,  B01L2300/0829 ,  B01L2300/087 ,  B01L2300/168 ,  B01L2400/0406 ,  B01L2400/0415 ,  B01L2400/0688 ,  C07B2200/11 ,  C40B40/06 ,  C40B40/10 ,  C40B40/12 ,  C40B50/14 ,  G01N21/253 ,  G01N21/645 ,  G01N35/1074 ,  G01N2021/6484 ,  G01N2035/1037

Abstract: Disclosed are systems and methods involved in extreme high throughput screening of compounds which have an affinity for a biological target. The system is based on a capillary bundle with two distinguishable ends wherein capillaries on one end are connected to compounds stored in discrete reservoirs and capillaries on the other end are bound and processed to form a two dimensional microarray. A capillary bundle having reaction wells for hybridization and compound reaction in one end of the capillaries is disclosed. The capillaries may be light-condicting. Also disclosed are various methods of identifying a target compound in a liquid using this capillary bundle as well as methods of fabricating the bundle. A novel surface tension guided reaction chamber is also provided. Methods and chemistry for fabrication and use of a surface tension guided reaction chamber in binding and hybridization assays are also disclosed. Methods and systems for precise metering of fluids within the capillaries and at the reaction chambers, including the surface tension guided "virtual" reaction well is provided. Methods for performing high throughput screens using optical fiber lined capillaries of the invention are also provided. The capillary arrays are used with both liquid probes as well as probes immobilized on the walls of the capillary.

Abstract translation: 公开了涉及对生物靶具有亲和力的化合物的极高通量筛选中涉及的系统和方法。 该系统基于毛细管束，其具有两个可区分的端部，其中一端的毛细管连接到存储在离散储存器中的化合物，另一端的毛细血管被结合并加工以形成二维微阵列。 公开了一种毛细管束，其具有在毛细血管的一端中用于杂交和化合物反应的反应孔。 毛细血管可能是轻微的。 还公开了使用该毛细管束在液体中鉴定目标化合物的各种方法以及制造束的方法。 还提供了一种新颖的表面张力引导反应室。 还公开了在结合和杂交测定中制造和使用表面张力引导反应室的方法和化学。 提供了用于精确计量毛细管内和反应室内的流体的方法和系统，包括表面张力引导的“虚拟”反应井。 还提供了使用本发明的光纤衬里毛细管进行高通量筛选的方法。 毛细管阵列与两个液体探针以及固定在毛细管壁上的探针一起使用。

公开(公告)号：WO01089680A1

公开(公告)日：2001-11-29

申请号：PCT/EP2001/004758

申请日：2001-04-27

IPC: B81B1/00 ,  B01J19/00 ,  B01L3/00 ,  C40B60/14

CPC classification number: B01L3/50853 ,  B01J19/0046 ,  B01J2219/00286 ,  B01J2219/00317 ,  B01J2219/00333 ,  B01J2219/00585 ,  B01L3/50857 ,  B01L2300/0819 ,  C40B60/14

Abstract: The invention relates to a device used in parallel microsynthesis. Microcompartments that are open on both sides are disposed in a flat arrangement in a microcompartment matrix (4) and are hermetically closed by pressure exerted on both sides by plungers (6) that are covered by a sealing film (11). A layer, produced from elastic material (9) and disposed between the plunger (6) and the sealing film (11), ensures that a continuous pressure is exerted on all microcompartments of the microcompartment matrix (4) that is loosely retained by a frame structure (1). Elevations (2) that project in both directions vertically to the plane of the microcompartment matrix (4) prevent the deformable thin layer from elastic material (9) from escaping laterally between the plunger (6) and the microcompartment matrix (4) while pressure is exerted. A plurality of microcompartment matrices (4) are simultaneously retained by the frame structure (1) and are hermetically closed by a corresponding number of associated plungers (6) that are fastened on a base plate (7) or a cover plate (8).

Abstract translation: 上的开口面积的在微腔阵列两侧（4）布置的微腔室被密封以相互压力到密封膜（11）覆盖，冲头（6）。 A中的冲头（6）和所述密封膜（11）之间布置的弹性材料层（9）确保在微腔阵列（4）的所有微室的均匀的压力，松由框架结构保持（1）。 防止在垂直于微腔阵列的平面（4）在两个方向上突出，微腔阵列（4）闭合周边凸起（2），该弹性材料的可变形薄层（9）的Anpressvorgangs冲头（6）和微室阵列之间横向期间 （4）射出。 几个Mikrokammermatrizen（4）同时由所述框架结构（1）保持并与连接到一个底板（7）和一个盖板（8）被固定，密封冲头（6）的相应的号码相关联。

公开(公告)号：WO0138583A3

公开(公告)日：2001-10-11

申请号：PCT/US0032208

申请日：2000-11-22

Applicant: DIVERSA CORP

Inventor： LAFFERTY WILLIAM MICHAEL ,  SHORT JAY M ,  KELLER MARTIN

IPC: G01N33/53 ,  B01J19/00 ,  B01L3/00 ,  C12M1/00 ,  C12M1/34 ,  C12N15/09 ,  C12N15/10 ,  C12Q1/02 ,  C40B40/06 ,  C40B60/14 ,  G01N27/447 ,  G01N33/15 ,  G01N33/50 ,  G01N37/00

CPC classification number: B82Y30/00 ,  B01J19/0046 ,  B01J2219/00351 ,  B01J2219/00367 ,  B01J2219/00416 ,  B01J2219/00479 ,  B01J2219/00481 ,  B01J2219/00522 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00617 ,  B01J2219/00621 ,  B01J2219/00659 ,  B01J2219/00677 ,  B01J2219/00686 ,  B01J2219/00689 ,  B01J2219/00702 ,  B01J2219/00722 ,  B01J2219/0074 ,  B01J2219/00743 ,  B01L3/5085 ,  B01L3/50857 ,  C12N15/1086 ,  C40B40/06 ,  C40B60/14

Abstract: Provided are methods of screening and identification of bioactivities and bioactive molecules of interest using a capillary array system. More specifically, disclosed are methods of using optical detection and capillary array-based techniques for screening libraries and recovering bioactive molecules having a desired activity or a nucleic acid sequence encoding such bioactive molecules.

Abstract translation: 提供使用毛细管阵列系统筛选和鉴定感兴趣的生物活性分子的方法。 更具体地，公开了使用光学检测和毛细管阵列技术来筛选文库和回收具有所需活性的生物活性分子或编码这种生物活性分子的核酸序列的方法。

公开(公告)号：WO01024929A1

公开(公告)日：2001-04-12

申请号：PCT/DE2000/003490

申请日：2000-10-04

IPC: G01N21/64 ,  B01L3/00 ,  C12M1/00 ,  C12N15/09 ,  C40B60/14 ,  G01N21/01 ,  G01N33/53 ,  G01N37/00 ,  G02B1/10

CPC classification number: B01L3/50857 ,  B01J2219/00317 ,  B01J2219/00497 ,  B01J2219/00608 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/00637 ,  B01J2219/00641 ,  B01L3/5027 ,  B01L2300/0819 ,  B01L2300/168 ,  C40B60/14 ,  G02B5/0833

Abstract: A surface of the pore (P) is provided with a coating for reflecting electromagnetic radiation. Said coating comprises one or more overlapping layers (A1, B1). If the substrate (1) consists of silicon, said layers (A1,l B1) are preferably made of materials which can be deposited in the course of CVD-processes. Said layers (A1, B1) are, for example, comprised of SiO2, polysilicon, silicon nitride or tungsten. The substrate (1) is, for example, provided as a component of a biochip used to detect fluorescing molecules (M) applied to the surface of pores (P)provided with said coating. The substrate (1) can be part of a fiber-optic light guide. Said coating is preferably constructed in such a way that electromagnetic radiation having a wavelength between 400 nm and 700 nm is optimally reflected.

Abstract translation: 所述孔（P）的表面设置有用于反射电磁辐射的涂层。 所述涂层由一个或多个叠置的层（A1，B1）。 如果（1）由硅制成的衬底，层（A1，B1）优选由可通过CVD工艺沉积的材料。 例如，Schicten（A1，B1）由二氧化硅，多晶硅，氮化硅或钨组成。 作为检测的所提供的与所述孔（P）的涂层表面的生物芯片的一部分的基板（1）中，例如施加荧光分子（M）。 在基板（1）可以是一个光导的一部分。 该涂层优选地被设计为使得其最佳地反射具有400至700nm之间的波长的电磁辐射。