公开(公告)号：WO2013096653A1

公开(公告)日：2013-06-27

申请号：PCT/US2012/071007

申请日：2012-12-20

Applicant: DANISCO US INC. ,  ESTELL, David A. ,  GRAYCAR, Thomas P. ,  JENSEN, Lene Bojsen ,  KELLETT-SMITH, Anja Hemmingsen ,  KRAGH, Karsten M. ,  MEJLDAL, Rie ,  PRICELIUS, Sina ,  YAO, Jian

Inventor： ESTELL, David A. ,  GRAYCAR, Thomas P. ,  JENSEN, Lene Bojsen ,  KELLETT-SMITH, Anja Hemmingsen ,  KRAGH, Karsten M. ,  MEJLDAL, Rie ,  PRICELIUS, Sina ,  YAO, Jian

IPC: C12N9/18

CPC classification number: C11D3/38627 ,  C07K2319/02 ,  C07K2319/50 ,  C12N9/20

Abstract: The present invention provides lipolytic enzyme variants. Specifically, the present invention provides lipolytic enzyme variants having two, three, or more modifications as compared to a parent lipolytic enzyme having at least one improved property. In addition, the present invention provides compositions comprising a lipolytic enzyme variant of the invention. The present invention also provides methods of cleaning using compositions comprising a lipolytic enzyme variant of the invention.

Abstract translation: 本发明提供脂肪分解酶变体。 具体而言，与具有至少一种改良性质的亲本脂肪分解酶相比，本发明提供了具有两个，三个或更多个修饰的脂肪分解酶变体。 此外，本发明提供了包含本发明的脂肪分解酶变体的组合物。 本发明还提供使用包含本发明的脂肪分解酶变体的组合物进行清洁的方法。

公开(公告)号：WO2009061380A4

公开(公告)日：2009-11-05

申请号：PCT/US2008012412

申请日：2008-11-03

Applicant: DANISCO US INC GENENCOR DIV ,  SHAW ANDREW ,  RAMER SANDRA ,  POWER SCOTT D ,  CHANG CLAUDINE ,  ESTABROOK MELODIE ,  JONES BRIAN E ,  CHOY CLEMENT ,  KOLKMAN MARC ,  LEEFLANG CHRIS ,  VROEMAN CASPER ,  WEYLER WALTER ,  KRAGH KARSTEN M ,  GOYAL MANSI ,  GRAYCAR THOMAS ,  HUANG VICTORIA

Inventor： SHAW ANDREW ,  RAMER SANDRA ,  POWER SCOTT D ,  CHANG CLAUDINE ,  ESTABROOK MELODIE ,  JONES BRIAN E ,  CHOY CLEMENT ,  KOLKMAN MARC ,  LEEFLANG CHRIS ,  VROEMAN CASPER ,  WEYLER WALTER ,  KRAGH KARSTEN M ,  GOYAL MANSI ,  GRAYCAR THOMAS ,  HUANG VICTORIA

IPC: C11D3/386 ,  C12N9/28 ,  C12N15/75

CPC classification number: C11D3/386 ,  C11D3/38681 ,  C12N9/2417

Abstract: Variants of Bacillus sp. TS-23 strain alpha-amylases exhibit improved enzymatic performance, including increased themostability, reduced calcium dependence, increased washing / cleaning performance, and baking ability. Compositions comprising these variants are useful in methods of starch processing, starch liquefaction, fermatation, starch saccharification, cleaning, laundrying, textile desizing, baking, and biofilm removal.

Abstract translation: 芽孢杆菌的变种 TS-23菌株α-淀粉酶表现出改善的酶性能，包括增加其稳定性，降低钙依赖性，提高洗涤/清洁性能和烘烤能力。 包含这些变体的组合物可用于淀粉加工，淀粉液化，粉化，淀粉糖化，清洗，洗衣，纺织品退浆，烘烤和生物膜除去的方法。

公开(公告)号：WO2009061380A8

公开(公告)日：2017-03-23

申请号：PCT/US2008012412

申请日：2008-11-03

Applicant: DANISCO US INC ,  SHAW ANDREW ,  RAMER SANDRA ,  POWER SCOTT D ,  CHANG CLAUDINE ,  ESTABROOK MELODIE ,  JONES BRIAN E ,  CHOY CLEMENT ,  KOLKMAN MARC ,  LEEFLANG CHRIS ,  VROEMEN CASPER ,  WEYLER WALTER ,  KRAGH KARSTEN M ,  GOYAL MANSI ,  GRAYCAR THOMAS ,  HUANG VICTORIA

Inventor： SHAW ANDREW ,  RAMER SANDRA ,  POWER SCOTT D ,  CHANG CLAUDINE ,  ESTABROOK MELODIE ,  JONES BRIAN E ,  CHOY CLEMENT ,  KOLKMAN MARC ,  LEEFLANG CHRIS ,  VROEMEN CASPER ,  WEYLER WALTER ,  KRAGH KARSTEN M ,  GOYAL MANSI ,  GRAYCAR THOMAS ,  HUANG VICTORIA

IPC: C11D3/386 ,  C12N9/28 ,  C12N15/75

CPC classification number: C11D3/386 ,  C11D3/38681 ,  C12N9/2417

Abstract: Variants of Bacillus sp. TS-23 strain alpha-amylases exhibit improved enzymatic performance, including increased themostability, reduced calcium dependence, increased washing / cleaning performance, and baking ability. Compositions comprising these variants are useful in methods of starch processing, starch liquefaction, fermatation, starch saccharification, cleaning, laundrying, textile desizing, baking, and biofilm removal.

Abstract translation: 芽孢杆菌的变种 TS-23菌株α-淀粉酶表现出改善的酶性能，包括增加其稳定性，降低钙依赖性，提高洗涤/清洁性能和烘烤能力。 包含这些变体的组合物可用于淀粉加工，淀粉液化，粉化，淀粉糖化，清洗，洗衣，纺织品退浆，烘烤和生物膜除去的方法。

公开(公告)号：WO2009061380A3

公开(公告)日：2009-05-14

申请号：PCT/US2008/012412

申请日：2008-11-03

Applicant: DANISCO US INC., GENENCOR DIVISION ,  SHAW, Andrew ,  RAMER, Sandra ,  POWER, Scott, D. ,  CHANG, Claudine ,  ESTABROOK, Melodie ,  JONES, Brian, E. ,  CHOY, Clement ,  KOLKMAN, Marc ,  LEEFLANG, Chris ,  VROEMAN, Casper ,  WEYLER, Walter ,  KRAGH, Karsten, M. ,  GOYAL, Mansi ,  GRAYCAR, Thomas ,  HUANG, Victoria

Inventor： SHAW, Andrew ,  RAMER, Sandra ,  POWER, Scott, D. ,  CHANG, Claudine ,  ESTABROOK, Melodie ,  JONES, Brian, E. ,  CHOY, Clement ,  KOLKMAN, Marc ,  LEEFLANG, Chris ,  VROEMAN, Casper ,  WEYLER, Walter ,  KRAGH, Karsten, M. ,  GOYAL, Mansi ,  GRAYCAR, Thomas ,  HUANG, Victoria

IPC: C11D3/386 ,  C12N9/28 ,  C12N15/75

Abstract: Variants of Bacillus sp. TS-23 strain alpha-amylases exhibit improved enzymatic performance, including increased themostability, reduced calcium dependence, increased washing / cleaning performance, and baking ability. Compositions comprising these variants are useful in methods of starch processing, starch liquefaction, fermatation, starch saccharification, cleaning, laundrying, textile desizing, baking, and biofilm removal.

公开(公告)号：WO2009061380A2

公开(公告)日：2009-05-14

申请号：PCT/US2008012412

申请日：2008-11-03

Applicant: DANISCO US INC GENENCOR DIV ,  SHAW ANDREW ,  RAMER SANDRA ,  POWER SCOTT D ,  CHANG CLAUDINE ,  ESTABROOK MELODIE ,  JONES BRIAN E ,  CHOY CLEMENT ,  KOLKMAN MARC ,  LEEFLANG CHRIS ,  VROEMAN CASPER ,  WEYLER WALTER ,  KRAGH KARSTEN M ,  GOYAL MANSI ,  GRAYCAR THOMAS ,  HUANG VICTORIA

Inventor： SHAW ANDREW ,  RAMER SANDRA ,  POWER SCOTT D ,  CHANG CLAUDINE ,  ESTABROOK MELODIE ,  JONES BRIAN E ,  CHOY CLEMENT ,  KOLKMAN MARC ,  LEEFLANG CHRIS ,  VROEMAN CASPER ,  WEYLER WALTER ,  KRAGH KARSTEN M ,  GOYAL MANSI ,  GRAYCAR THOMAS ,  HUANG VICTORIA

IPC: C11D3/386 ,  C12N9/28 ,  C12N15/75

CPC classification number: C11D3/386 ,  C11D3/38681 ,  C12N9/2417

Abstract: Variants of Bacillus sp. TS-23 strain alpha-amylases exhibit improved enzymatic performance, including increased themostability, reduced calcium dependence, increased washing / cleaning performance, and baking ability. Compositions comprising these variants are useful in methods of starch processing, starch liquefaction, fermatation, starch saccharification, cleaning, laundrying, textile desizing, baking, and biofilm removal.

Abstract translation: 芽孢杆菌的变种 TS-23菌株α-淀粉酶表现出改善的酶性能，包括增加其稳定性，降低钙依赖性，提高洗涤/清洁性能和烘烤能力。 包含这些变体的组合物可用于淀粉加工，淀粉液化，粉化，淀粉糖化，清洗，洗衣，纺织品退浆，烘烤和生物膜除去的方法。

公开(公告)号：WO9931959A2

公开(公告)日：1999-07-01

申请号：PCT/US9827213

申请日：1998-12-21

Applicant: GENENCOR INT ,  PLANK PETER F ,  GRAYCAR THOMAS P ,  FLYNN MATHEW J

Inventor： PLANK PETER F ,  GRAYCAR THOMAS P ,  FLYNN MATHEW J

IPC: C11D3/386 ,  C12Q1/00 ,  C12Q1/34 ,  C12Q1/37 ,  G01N17/00 ,  G01N33/15 ,  G01N33/52

CPC classification number: G01N33/52 ,  C11D3/38672 ,  C12Q1/00 ,  C12Q1/34 ,  C12Q1/37

Abstract: Stability test comprising mixing granulated protein (e.g. enzyme) and a detergent matrix, incubating the mixture at high temperature and humidity, and assaying a sample of the mixture for protein stability.

Abstract translation: 稳定性测试包括混合造粒蛋白质（例如酶）和洗涤剂基质，在高温和高湿下孵育该混合物，并测定该混合物样品的蛋白质稳定性。

公开(公告)号：WO1997033001A1

公开(公告)日：1997-09-12

申请号：PCT/US1997003411

申请日：1997-03-06

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA ,  GENENCOR INTERNATIONAL, INC. ,  HSIEH, You-lo ,  HARTZELL, Mary, Michele ,  BOSTON, Matthew, G. ,  CLARKSON, Kathleen, A. ,  COLLIER, Katherine, D. ,  GRAYCAR, Thomas, P. ,  LARENAS, Edmund, A.

Inventor： THE REGENTS OF THE UNIVERSITY OF CALIFORNIA ,  GENENCOR INTERNATIONAL, INC.

IPC: C12S11/00

CPC classification number: D06M16/003 ,  D06M2101/06 ,  D06M2101/32 ,  D06M2200/00 ,  Y10S8/04

Abstract: Textile fibers are treated with enzymes in the absence of surfactants, with the effect of increasing the wettability and absorbency of the fibers. The enzymes are pectinases, cellulases, proteases, lipases or combinations thereof. The wetting properties of cotton fibers are found to be most substantially improved by treatment with a mixture of cellulase and pectinase. The effects of five hydrolyzing enzymes on improving the hydrophilicity of several polyester fabrics have been studied. Four out of the five lipases studied improve the water wetting and absorbent properties of the regular polyester fabrics more than alkaline hydrolysis under optimal conditions (3N NaOH at 55 DEG C for 2 hours). Compared to aqueous hydrolysis, the enzyme reactions have shown to be effective under more moderate conditions, including a relatively low concentration (0.01 g/L), a shorter reaction time (10 minutes), at an ambient temperature (25 DEG C). Contrary to the results with alkaline hydrolysis, the improved water wettability is accompanied by full strength retention. Lipase has also shown to be effective in improving the wetting and absorbent properties of sulfonated polyester and microdenier polyester fabrics.

Abstract translation: 纺织纤维在没有表面活性剂的情况下用酶处理，具有增加纤维的润湿性和吸收性的作用。 酶是果胶酶，纤维素酶，蛋白酶，脂肪酶或其组合。 通过用纤维素酶和果胶酶的混合物处理，发现棉纤维的润湿性能最显着地提高。 已经研究了五种水解酶对改善几种聚酯织物的亲水性的影响。 所研究的五种脂肪酶中的四种在最佳条件下（3N NaOH在55℃下2小时）改善了常规聚酯织物的水润湿性和吸水性，而不是碱性水解。 与水解水解相比，酶反应在环境温度（25℃）下，在更温和的条件下有效，包括较低的浓度（0.01g / L），较短的反应时间（10分钟）。 与碱性水解的结果相反，改善的水润湿性伴随着完全的强度保持。 脂肪酶也显示出有效地改善了磺化聚酯和微粒聚酯织物的润湿和吸收性能。