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    SYNTHESIS OF CODON RANDOMIZED NUCLEIC ACIDS
    4.
    发明申请
    SYNTHESIS OF CODON RANDOMIZED NUCLEIC ACIDS 审中-公开
    基于密码子随机生成的核酸合成

    公开(公告)号：WO00018778A1

    公开(公告)日：2000-04-06

    申请号：PCT/US1999/022436

    申请日：1999-09-28

    IPC: C07H21/00 ,  C07H1/00 ,  C07C255/11 ,  C07C255/49 ,  C07H1/02

    CPC classification number: C07H21/00 ,  C12N15/1058 ,  Y02P20/55

    Abstract: A method for generating a selected set of codons is disclosed; the method includes the steps of: (a) providing a first set of mononucleosides, mononucleotides, dinucleotides, or mixture thereof, where a subset A of the first set is protected with a protecting group A', and a subset B of the first set is protected with a protecting group B', where A' and B' are orthogonal protecting groups; (b) selectively removing the protecting group A' from subset A; (c) coupling the products of step (b) with a second set of mononucleosides, mononucleotides, dinucleotides, or a mixture thereof, where the second set is protected with protecting group A'; (d) optionally removing protecting group A' from the products of step (c); (e) optionally coupling the products of step (d) with a third set of mononucleosides, where the third set is protected with protecting group A'; (f) selectively removing the protecting group B' from subset B; (g) coupling the products of step (f) with a fourth set of mononucleosides, mononucleotides, dinucleotides, or a mixture thereof, where the fourth set is protected with protecting group A' or protecting group B'; (h) optionally selectively removing protecting group B' from the products of step (g); and (i) optionally coupling the products of step (h) with a fifth set of mononucleosides, to yield a selected set of codons.

    Abstract translation: 本发明涉及产生选定的一组密码子的方法。 该方法包括：（a）提供的第一组由mononucleoside，单核苷酸，二核苷酸或它们的混合物的，所述包括第一子集的集合A被保护基团A“和一个子组B保护 由保护基B'保护，A'和B'为正交保护基; （b）从子集A选择性提取保护基A'; （c）将步骤（b）的产物偶联至第二组单核苷酸，单核苷酸，二核苷酸或其混合物，所述第二组受保护基团A'保护; （d）任选地从步骤（c）的产物中提取保护基团A'; （e）任选将步骤（d）的产物偶联至受保护基A'保护的第三组单核苷; （f）从子集B中选择性地提取保护基团B'; （G）连接步骤（f）与由mononucleoside，单核苷酸，二核苷酸或它们的混合物的第四组的产品，所述第四组件由保护基团A“或保护基团保护 B“; （h）任选任选地从步骤（g）的产物中提取保护基团B'; 和（i）任选将步骤（h）的产物偶联至第五组单核苷酸以产生选定的一组密码子。

    SYNTHESIS OF CODON RANDOMIZED NUCLEIC ACIDS
    6.
    发明申请
    SYNTHESIS OF CODON RANDOMIZED NUCLEIC ACIDS 审中-公开
    CODON RANDOMIZED NUCLEIC ACIDS的合成

    公开(公告)号：WO0018778A9

    公开(公告)日：2000-06-08

    申请号：PCT/US9922436

    申请日：1999-09-28

    Applicant: PHYLOS INC

    Inventor： LOHSE PETER ,  KUIMELIS ROBERT G

    IPC: C07H21/00 ,  C07H1/00 ,  C07C255/11 ,  C07C255/49 ,  C07H1/02

    CPC classification number: C07H21/00 ,  C12N15/1058 ,  Y02P20/55

    Abstract: A method for generating a selected set of codons is disclosed; the method includes the steps of: (a) providing a first set of mononucleosides, mononucleotides, dinucleotides, or mixture thereof, where a subset A of the first set is protected with a protecting group A', and a subset B of the first set is protected with a protecting group B', where A' and B' are orthogonal protecting groups; (b) selectively removing the protecting group A' from subset A; (c) coupling the products of step (b) with a second set of mononucleosides, mononucleotides, dinucleotides, or a mixture thereof, where the second set is protected with protecting group A'; (d) optionally removing protecting group A' from the products of step (c); (e) optionally coupling the products of step (d) with a third set of mononucleosides, where the third set is protected with protecting group A'; (f) selectively removing the protecting group B' from subset B; (g) coupling the products of step (f) with a fourth set of mononucleosides, mononucleotides, dinucleotides, or a mixture thereof, where the fourth set is protected with protecting group A' or protecting group B'; (h) optionally selectively removing protecting group B' from the products of step (g); and (i) optionally coupling the products of step (h) with a fifth set of mononucleosides, to yield a selected set of codons.

    Abstract translation: 公开了一种用于产生选定密码子集的方法; 该方法包括以下步骤：（a）提供第一组单核苷，单核苷酸，二核苷酸或其混合物，其中第一组的子集A被保护基团A'和第一组的子集B 被保护基B'保护，其中A'和B'是正交保护基; （b）从子集A中选择性地除去保护基团A'; （c）将步骤（b）的产物与第二组单核苷，单核苷酸，二核苷酸或其混合物偶联，其中第二组用保护基A'保护; （d）任选地将步骤（c）的产物中的保护基A'除去; （e）任选地将步骤（d）的产物与第三组单核苷类偶联，其中第三组用保护基A'保护; （f）从子集B选择性地除去保护基B'; （g）步骤（f）的产物与第四组单核苷，单核苷酸，二核苷酸或其混合物偶联，其中第四组用保护基A'或保护基B'保护; （h）任选地选择性地从步骤（g）的产物中除去保护基B'; 和（i）任选地将步骤（h）的产物与第五组单核苷化合物偶联，得到选定的一组密码子。

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