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41 条记录 (耗时 0.026 秒)

    TRANSAMINASE MUTANT AND USE THEREOF
    16.
    发明公开
    TRANSAMINASE MUTANT AND USE THEREOF 审中-公开

    公开(公告)号：EP3677674A1

    公开(公告)日：2020-07-08

    申请号：EP17930421.7

    申请日：2017-11-06

    申请人： Asymchem Life Science (Tianjin) Co., Ltd.

    发明人： HONG, Hao ,  JAMES, Gage ,  LU, jiangping ,  JIAO, Xuecheng ,  ZHANG, Na ,  LI, Rui ,  ZHANG, Kejian

    IPC分类号： C12N9/10 ,  C12N15/63 ,  C12P13/00 ,  C12N15/54

    摘要： Disclosed are transaminase mutants and use thereof. The amino acid sequence of the transaminase mutant is obtained by the mutation of the amino acid sequence as shown in SEQ ID NO: 1, and the mutation at least comprises one of the following mutation sites: the 19-th site, the 41-th site, the 43-th site, the 72-th site, the 76-th site, the 92-th site, the 107-th site, the 125-th site, the 132-th site, the 226-th site, the 292-th site, the 295-th site, the 308-th site, and the 332-th site; and the 19-th site is mutated into a serine, the 41-th site is mutated into a serine, the 43-th site is mutated into an asparagine, a glycine in the 72-th site is mutated into a leucine, etc.; or the amino acid sequence of the transaminase mutant has the mutation sites in the mutanted amino acid sequence, and has more than 80% homology to the mutanted amino acid sequence.

    TRANSAMINASE AND USE THEREOF
    19.
    发明公开
    TRANSAMINASE AND USE THEREOF 审中-公开
    TRANSAMINASE UND VERWENDUNG DAVON

    公开(公告)号：EP3075847A4

    公开(公告)日：2017-08-02

    申请号：EP14865656

    申请日：2014-10-31

    申请人： ASYMCHEM LABORATORIES (TIANJIN) CO LTD ,  ASYMCHEM LIFE SCIENCE (TIANJIN) CO LTD ,  TIANJIN ASYMCHEM PHARMACEUTICAL CO LTD ,  ASYMCHEM LABORATORIES (FUXIN) CO LTD ,  JILIN ASYMCHEM LABORATORIES CO LTD

    发明人： HONG HAO ,  GAO FENG ,  LI YANJUN ,  ZHANG YAN ,  LI SHAOHE

    IPC分类号： C12N9/10 ,  C12N15/54 ,  C12P13/00 ,  C12P17/12 ,  C12R1/01 ,  C12R1/66

    CPC分类号： C12P13/001 ,  C12N9/1096 ,  C12N15/70 ,  C12P17/12 ,  C12Y206/01

    摘要： Atransaminase and a use thereof are provided. The transaminase has the amino acid sequences as shown in SEQ ID NO: 2 or 4, or has at least 80% identity to the amino acid sequences as shown in SEQ ID NO: 2 or 4, or has amino acid sequences which are obtained by the substitution, deletion or addition of one or more amino acids and have an the activity of an omega-transaminase with high stereoselective R-configuration catalytic activity, wherein the high stereoselective refers to the content of one of the stereoisomers being at least about 1.1 times that of the other. The transaminase can synthesize a chiral amine of R-configuration with a relatively high chiral purity, and is therefore suitable for the industrial use of the synthesis of chiral amines.

    摘要翻译： 提供了转氨酶及其用途。 转氨酶具有如SEQ ID NO：2或4所示的氨基酸序列，或与SEQ ID NO：2或4所示的氨基酸序列具有至少80％的同一性，或具有通过以下方式获得的氨基酸序列： 一个或多个氨基酸的取代，缺失或添加并且具有具有高立体选择性R-构型催化活性的ω-转氨酶的活性，其中所述高立体选择性是指其中一种立体异构体的含量为至少约1.1倍 那另一个。 转氨酶可以合成具有较高手性纯度的R-构型手性胺，因此适用于合成手性胺的工业应用。

    OMEGA-TRANSAMINASE OF R CONFIGURATION AND USE THEREOF
    20.
    发明公开
    OMEGA-TRANSAMINASE OF R CONFIGURATION AND USE THEREOF 审中-公开
    OMEGA-TRANSAMINASE MIT R-KONFIGURATION UND DAVON VERWENDUNG

    公开(公告)号：EP3075856A1

    公开(公告)日：2016-10-05

    申请号：EP14865169.8

    申请日：2014-10-29

    申请人： Asymchem Laboratories (Tianjin) Co., Ltd ,  Asymchem Life Science (Tianjin) Co., Ltd ,  Tianjin Asymchem Pharmaceutical Co., Ltd ,  Asymchem Laboratories (Fuxin) Co., Ltd ,  Jilin Asymchem Laboratories Co., Ltd

    发明人： HONG, Hao ,  GAO, Feng ,  LI, Yanjun ,  ZHANG, Yan ,  LI, Shaohe

    IPC分类号： C12N15/54 ,  C12N15/31 ,  C12N15/11 ,  C12N9/10 ,  C12P41/00 ,  C07K14/195

    CPC分类号： C12N9/1096 ,  C12P13/001 ,  C12P17/12 ,  C12P41/006 ,  C12Y206/01

    摘要： An omega-transaminase of R-configuration is provided. The omega-transaminase of R-configuration has the amino acid sequence as shown in SEQ ID NO: 2, or has at least 80% identity to the amino acid sequence as shown in SEQ ID NO: 2, or has the amino acid sequence of proteins which have substituted, deleted or added one or more amino acids and have the activity of an omega-transaminase with a high stereoselectivity R-configuration catalytic activity; and does not have the amino acid sequence encoded by the nucleotide sequence as shown in SEQ ID NO: 4. The high stereoselectivity refers to the content of one of the stereoisomers being at least about 1.1 times that of the other. A use of the omega-transaminase of R-configuration is provided, which can be used for highly efficient synthesis of a chiral amine of R-configuration with a relatively high chiral purity, and is therefore suitable for industrial production of chiral amines.

    摘要翻译： 提供R-构型的ω-转氨酶。 R构型的ω-转氨酶具有如SEQ ID NO：2所示的氨基酸序列，或与SEQ ID NO：2所示的氨基酸序列具有至少80％的同一性，或具有SEQ ID NO：2所示的氨基酸序列 具有取代，缺失或添加一个或多个氨基酸并且具有高立体选择性R构型催化活性的ω-转氨酶活性的蛋白质; 并且不具有由SEQ ID NO：4所示的核苷酸序列编码的氨基酸序列。高立体选择性是指一种立体异构体的含量为至少约1.1倍。 提供R-构型的ω-转氨酶的用途，其可用于高效合成手性纯度较高的R-构型手性胺，因此适用于手性胺的工业生产。