摘要:
The present invention relates to a process for producing an organic acid by fermentation, comprising the process steps: I) cultivating microorganisms in a culture medium to which are fed, as assimilable carbon sources, glycerol and a further carbonaceous compound, to allow the microorganisms to produce the organic acid, thereby obtaining a fermentation broth comprising the organic acid; II) recovering the organic acid or the salt thereof from the fermentation broth obtained in process step I); wherein, at least for a certain period of time in process step I), the consumption rate of the further carbonaceous compound (CRc.c.; in g per liter per hour) is lower than the maximum theoretical consumption rate of the further carbonaceous compound (CRc.c. max; in g per liter per hour).
摘要翻译:本发明涉及通过发酵生产有机酸的方法,包括以下工艺步骤:I)在作为可同化碳源进料的培养基中培养微生物甘油和另外的碳质化合物,以使微生物 产生有机酸,从而得到包含有机酸的发酵液; II)从方法步骤I)中获得的发酵液中回收有机酸或其盐; 其中,至少在处理步骤I)中的一段时间内,进一步的碳质化合物(CRc.c;以g / l / h)的消耗速率低于进一步的碳质化合物的最大理论消耗速率 (CRc.c.max;以g每升/小时计)。
摘要:
The present invention relates to a method of producing riboflavin in a genetically modified organism of the genus Eremothecium, wherein said genetic modification is linked to the fatty acid uptake and/or beta-oxidation pathway of said organism, comprising growing said organisms in a culture medium and isolating riboflavin from the culture medium. The invention further relates to a method of providing a riboflavin accumulating organism belonging to the genus Eremothecium by genetically modifying said organism, to organisms obtained by such a method, as well as the use of such genetically modified organisms for increasing the accumulation of riboflavin.
摘要:
The present invention relates to the use of recombinant microorganisms comprising DNA molecules in a deregulated form which improve the production of cadaverine, as well as to recombinant DNA molecules and polypeptides used to produce the microorganism, said microorganism comprising an intracellular lysine decarboxylase activity and an enhanced lysine import activity or comprising an intracellular and an extracellular lysine decarboxylase activity or comprising an intracellular and extracellular lysine decarboxylase activity and an enhanced lysine import activity. The present invention also relates to a processes for the production of cadaverine using recombinant microorganisms.
摘要:
The present invention relates to a method of increasing the amount of at least one polypeptide in the host cell by expressing a modified nucleotide sequence encoding for a polypeptide in a host cell with said modified.nucleotide sequence being derived from a different non-modified nucleotide sequence encoding for a polypeptide of identical amino acid sequence such that the codon usage of the modified nucleotide sequence is adjusted to the codon usage of abundant proteins in the host cell.
摘要:
The present invention concerns methods for the production of microorganisms with increased efficiency for methionine synthesis. The present invention also concerns microorganisms with increased efficiency for methionine synthesis. Furthermore, the present invention concerns methods for determining the optimal metabolic flux for organisms with respect to methionine synthesis.
摘要:
The present invention relates to a modified microorganism having, compared to its wildtype,—a reduced activity of an enzyme encoded by the ptsA-gene,—a reduced activity of an enzyme encoded by the ptsH-gene or—a reduced activity of an enzyme encoded by the ptsA-gene and a reduced activity of an enzyme encoded by the ptsH-gene, wherein the wildtype from which the modified microorganism has been derived belongs to the family of Pasteurellaceae. The present invention also relates to a method for producing succinic acid and to the use of modified microorganisms.
摘要:
The present invention relates to genetically modified microorganisms capable of producing beta-glucans, characterized in that said genetically modified microorganism overexpresses (i) a polynucleotide encoding a polypeptide having 1,3-&bgr;-D-glucan synthase-activity, and/or (ii) a polypeptide having 1,3-&bgr;-D-glucan synthase-activity, compared to a corresponding non-modified control microorganism of the same strain. The present invention also relates to the use of a polynucleotide encoding a polypeptide having 1,3-&bgr;-D-glucan synthase-activity or the use of such a polypeptide for producing &bgr;-glucans. Furthermore, the present invention relates to methods for producing &bgr;-glucans comprising the introduction of a promoter upstream of a polynucleotide encoding a polypeptide having 1,3-&bgr;-D-glucan synthase-activity thereby increasing the expression of said polynucleotide, or a polynucleotide encoding a polypeptide having 1,3-&bgr;-D-glucan synthase-activity into a microorganism being able to synthesize &bgr;-glucans.
摘要:
The present invention relates to a novel method for the fermentative production of dipicolinate by cultivating a recombinant microorganism expressing an enzyme having dipicolinate synthetase activity. The present invention also relates to corresponding recombinant hosts, recombinant vectors, expression cassettes and nucleic acids suitable for preparing such hosts as well as a method of preparing polyester or polyamide copolymers making use of dipicolinate as obtained by fermentative production.