公开(公告)号：EP2580319A1

公开(公告)日：2013-04-17

申请号：EP11728211.1

申请日：2011-06-14

申请人： Cellartis AB

发明人： JENSEN, Janne

IPC分类号： C12N5/071

CPC分类号： C12N5/0671 ,  C12N5/067 ,  C12N2501/999 ,  C12N2506/02 ,  C12N2533/50

摘要： The present invention relates to the use of 3-dimensional (3D) synthetic or animal-derived bioscaffolds as substrates for the improved growth and differentiation of hPS (Human pluripotent stem cells); these scaffolds being adapted for use in conjunction with existing cell culture lab plastic-ware. More specifically, it relates to the seeding of these scaffolds, either alone or in conjunction with various biologic matrix coatings, with hPS cells for the improved differentiation of said hPS cells into hepatocyte or hepatocyte-like cell types. The invention also relates to the seeding of partially-differentiated hepatocyte progenitors onto scaffolds for further differentiation into more mature hepatocyte-cell types.

摘要翻译： 本发明涉及使用三维（3D）的合成或动物来源的生物支架作为底物用于HPS的改进的生长和分化（人多能干细胞）的; 论文支架是与现有的细胞培养实验室塑料餐具连词angepasst的使用。 更具体地，本发明涉及支架论文，无论是单独使用或与各种生物基质涂层连词的接种，用HPS细胞用于细胞分化改进为肝。所述HPS或肝细胞样细胞类型。 因此，本发明涉及部分地分化的肝细胞的祖细胞的走上支架用于进一步分化成更成熟的肝细胞的细胞类型接种。

公开(公告)号：EP2444477A1

公开(公告)日：2012-04-25

申请号：EP10789497.4

申请日：2010-06-15

申请人： Shiseido Company, Ltd.

发明人： FUJIWARA, Shigeyoshi ,  TAKEI, Keigo ,  HIRAYAMA, Aya ,  SUDA, Yukimitsu

IPC分类号： C12M1/00 ,  C12M3/00 ,  C12N5/07 ,  C08J7/04 ,  C08J7/12 ,  C09D133/14

CPC分类号： C12M23/20 ,  C12N5/062 ,  C12N5/0667 ,  C12N5/0671 ,  C12N5/0693 ,  C12N2533/30 ,  G01N33/5082

摘要： A container for forming a cell aggregate is provided in which a group represented by a general formula of:

(in the formula, each of R 1 , R 2 , and R 3 is independently a an alkyl group with a carbon number of 1 or more and 6 or less and m is an integer of 2 or more and 6 or less.) and at least one of an amino group, a carboxyl group, and a hydroxyl group are present near a surface thereof.

摘要翻译： 提供一种用于形成细胞凝集块的容器，其中由通式表示的基团：（式中，各R 1，R 2，和R 3中的被unabhängig一个具有1个或更多碳数的烷基组 和小于或等于6，m是2以上，6以下。）和氨基，羧基中的至少一种的整数，和羟基是存在于其表面附近。

公开(公告)号：EP2412800A1

公开(公告)日：2012-02-01

申请号：EP10171265.1

申请日：2010-07-29

申请人： Koninklijke Nederlandse Akademie van Wetenschappen

发明人： Ortega, Meritxell Huch ,  Clevers, Johannes Carolus

IPC分类号： C12N5/074

CPC分类号： C12N5/0671 ,  A61K35/407 ,  C12N5/0672 ,  C12N2500/38 ,  C12N2501/11 ,  C12N2501/119 ,  C12N2501/12 ,  C12N2501/155 ,  C12N2501/415 ,  C12N2533/90

摘要： The invention relates to a liver organoid, uses thereof and method for obtaining them.

摘要翻译： 本发明涉及肝类器官，它们和方法使用用于获得它们。

公开(公告)号：EP2027257A4

公开(公告)日：2010-10-20

申请号：EP07748692

申请日：2007-05-24

申请人： AGENCY SCIENCE TECH & RES

发明人： DU YANAN ,  HAN RONGBIN ,  YU HANRY

IPC分类号： C12M3/00 ,  C12N5/071

CPC分类号： C12N5/0677 ,  C12N5/0068 ,  C12N5/0671 ,  C12N2533/20 ,  C12N2533/30 ,  C12N2533/50 ,  C12N2533/52 ,  C12N2533/54

摘要： The invention provides a surface, wherein said surface comprises (i) a polymer substrate and (ii) sugar groups and peptide groups coupled to said substrate suitable for culturing hepatocytes.

公开(公告)号：EP1966367B1

公开(公告)日：2010-03-03

申请号：EP06828762.2

申请日：2006-12-22

申请人： Drugmode ApS

发明人： LARSEN,Peter Mose ,  FEY, Stephen John

IPC分类号： C12M3/02 ,  C12M3/06 ,  G01N33/50 ,  C12Q1/68 ,  C12N5/07 ,  C12M3/04

CPC分类号： G01N33/502 ,  C12M23/16 ,  C12M27/10 ,  C12M29/04 ,  C12N5/0671 ,  C12Q1/6809

摘要： The present invention provides a bioreacto 1r for incubation of cell cultures, tissue biopsies, cell clusters, tissue-like structures, 'prototissues' or similar samples. The bioreactor is generally adapted for rotation for use in microgravity conditions and equipped with an incubation cavity having a small internal fluid volume, generally less than 1 ml. To avoid problems associated with small volume incubation, the bioreactor may include a humidity chamber or other means of avoiding dehydration as well as substantially fluid-tight closures for access ports that avoid introduction of air bubbles to the incubation cavity. The small-volume bioreactor permits long term maintenance of tissue differentiation states in cultures. Also provided are methods of incubating cells or tissues using the bioreactor including methods of creating molecular profiles of biological effects of chemical compositions on differentiated cell or tissue samples maintained in long term culture.

公开(公告)号：EP2084264A1

公开(公告)日：2009-08-05

申请号：EP08726608.6

申请日：2008-03-07

申请人： Corning Incorporated

发明人： NOLL, Frederick, E. ,  SENARATNE, Wageesha ,  WEI, Ying

IPC分类号： C12N5/00 ,  C12N5/06

CPC分类号： C12N5/0068 ,  C12N5/0671 ,  C12N2533/70

摘要： This invention relates three dimensional porous cell culture matrices or scaffolds. More particularly, this invention relates to three dimensional porous cell culture matrices or scaffolds for cell culture which are derived from or contain gums including naturally occurring gums, plant gums, galactomannan gums or derivatives thereof. The invention also relates to articles of manufacture (e.g., cell culture vessels and labware) having such matrices or scaffolds, methods of applying these materials to cell culture surfaces, and methods of using cell culture vessels having these three dimensional porous cell culture matrices or scaffolds.

公开(公告)号：EP1939280A1

公开(公告)日：2008-07-02

申请号：EP08005978.5

申请日：2002-07-25

申请人： Transparent Inc.

发明人： The designation of the inventor has not yet been filed

IPC分类号： C12N5/06

CPC分类号： C12N5/0671 ,  C12N5/0697 ,  C12N2502/14 ,  C12N2502/28 ,  C12N2533/30 ,  C12N2533/40 ,  C12N2535/10

摘要： Parenchymal cells are cultivated on cultivated endothelial cells or cultivated fibroblasts which have been separated by a surface of a specific hydrophilic polymer, and which have been patterned. A culture which contains thus formed patterned spheroids of cultivated parenchymal cells is thereby provided by this invention. This culture maintains a function which is specific to the parenchymal cells over a long period of time.

摘要翻译： 将实质细胞培养在培养的内皮细胞或培养的成纤维细胞上，所述成纤维细胞已被特定亲水性聚合物的表面分离，并已经被图案化。 因此本发明提供了由此形成的培养的实质细胞的图案化球体的培养物。 该培养物保持长时间对实质细胞特异的功能。

公开(公告)号：EP1668118A2

公开(公告)日：2006-06-14

申请号：EP04783636.6

申请日：2004-09-10

申请人： Becton, Dickinson and Company

发明人： GUARINO, Richard, David ,  PRESNELL, Sharon, C. ,  LIEBMANN-VINSON, Andrea ,  HEIDARAN, Mohammad, A. ,  HEMPERLY, John, J. ,  JURGENSEN, Stewart ,  LAETHEM, Ronald, M. ,  ROBBINS, Neil

IPC分类号： C12N5/06 ,  C12N5/08 ,  G01N33/50

CPC分类号： G01N33/5067 ,  C12N5/067 ,  C12N5/0671 ,  C12N2533/32 ,  C12N2533/54

摘要： Surfaces useful for cell culture comprise a support to which is bound a CAR material, and, bound to the CAR material, an ECM protein, or a biologically active fragment or variant thereof such as elastin, fibronectin, vitronectin, laminin, collagen I, collagen III, collagen IV, and collagen VI. Also, optionally present on the surface is an active factor, preferably a polycationic polymer or a biologically active fragment or variant thereof, such as polyethyleneimine (PEI), poly-D-lysine (PDL), poly-L-lysine (PLL), poly-D-ornithine (PDO) or poly-L-ornithine (PLO). This surface is used in cell culture to promote cell attachment, survival, maintenance of function, and/or proliferation of primary liver cells. The invention also relates to methods utilizing this surface, such as methods for attachment, survival, maintenance of function, and/or proliferation of cells. Further disclosed is the use of the surface in cell culture with serum-free medium. Methods of screening using the surface of the invention are also disclosed.

公开(公告)号：EP1430111A1

公开(公告)日：2004-06-23

申请号：EP02757586.9

申请日：2002-09-05

申请人： Geron Corporation

发明人： MANDALAM, Ramkumar ,  XU, Chunhui ,  GOLD, Joseph, D. ,  CARPENTER, Melissa, K.

IPC分类号： C12N5/00

CPC分类号： C12N15/1096 ,  C12N5/0062 ,  C12N5/0068 ,  C12N5/0075 ,  C12N5/0603 ,  C12N5/0606 ,  C12N5/0607 ,  C12N5/0619 ,  C12N5/0622 ,  C12N5/0662 ,  C12N5/0671 ,  C12N5/0672 ,  C12N5/0678 ,  C12N5/068 ,  C12N5/0692 ,  C12N5/0693 ,  C12N5/0696 ,  C12N11/04 ,  C12N15/1034 ,  C12N2500/25 ,  C12N2500/90 ,  C12N2500/98 ,  C12N2500/99 ,  C12N2501/065 ,  C12N2501/105 ,  C12N2501/115 ,  C12N2501/119 ,  C12N2501/125 ,  C12N2501/13 ,  C12N2501/145 ,  C12N2501/155 ,  C12N2501/2306 ,  C12N2501/235 ,  C12N2501/237 ,  C12N2501/26 ,  C12N2501/998 ,  C12N2502/13 ,  C12N2502/99 ,  C12N2503/02 ,  C12N2506/02 ,  C12N2510/00 ,  C12N2510/04 ,  C12N2533/50 ,  C12N2533/90

摘要： This disclosure provides an improved system for culturing human pluripotent stem cells. Traditionally, pluripotent stem cells are cultured on a layer of feeder cells (such as mouse embryonic fibroblasts) to prevent them from differentiating. In the system described here, the role of feeder cells is replaced by components added to the culture environment that support rapid proliferation without differentiation. Effective features are a suitable support structure for the cells, and an effective medium that can be added fresh to the culture without being preconditioned by another cell type. Culturing human embryonic stem cells in fresh medium according to this invention causes the cells to expand surprisingly rapidly, while retaining the ability to differentiate into cells representing all three embryonic germ layers. This new culture system allows for bulk proliferation of pPS cells for commercial production of important products for use in drug screening and human therapy.

公开(公告)号：EP1406680A1

公开(公告)日：2004-04-14

申请号：EP02736456.1

申请日：2002-06-14

申请人： Johns Hopkins Singapore Pte Ltd

发明人： HAI-QUAN, Mao,Block 60 West Coast Crescent ,  KUAN, Chee, Mun,41 Science Park Road ,  LEONG, Kam, Weng

IPC分类号： A61L27/54 ,  A61L27/56 ,  C12N5/00 ,  A61K47/48

CPC分类号： A61K9/70 ,  A61K47/593 ,  A61K47/605 ,  A61K47/6953 ,  A61L27/28 ,  A61L27/38 ,  A61L27/50 ,  A61L27/54 ,  A61L2300/214 ,  A61L2300/22 ,  A61L2300/23 ,  A61L2300/258 ,  A61L2300/412 ,  C12N5/0068 ,  C12N5/0671 ,  C12N2533/30 ,  Y10T442/2525

摘要： The present invention is directed to surface functionalization of polymeric fibers. Surface biofunctionalization is achieved by covalent conjugation of biofunctional igands and/or cell growth factors that are crucial for cell attachment, proliferation and functions. Biofunctional fibers could be fabricated into three-dimensional scaffolds. Polymer fibers described here comprise of biocompatible polymers that are either biodegradable ornon-biodegradable. This patent also describes a series of new biodegradable polyphosphoramidates for the processing of biodegradable fibers. Scaffolds made of non-biodegradable functional fibers could be used for in vitro cell culture (for example, ex vivo cell expansion), while biodegradable functional fibers could be fabricated into tissue engineering scaffolds.