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    THERMOSTABLE BIOCATALYST COMBINATION FOR NUCLEOSIDE SYNTHESIS
    2.
    发明公开
    THERMOSTABLE BIOCATALYST COMBINATION FOR NUCLEOSIDE SYNTHESIS 有权
    用于核苷酸合成耐热Biokatalysatorkombination

    公开(公告)号：EP2516637A1

    公开(公告)日：2012-10-31

    申请号：EP10798096.3

    申请日：2010-12-22

    申请人： Plasmia Biotech, S.L.

    发明人： MONTILLA AREVALO, Rafael ,  DERONCELÉ THOMAS, Víctor, Manuel ,  LÓPEZ GÓMEZ, Cristina ,  PASCUAL GILABERT, Marta ,  ESTÉVEZ COMPANY, Carlos ,  CASTELLS BOLIART, Josep

    IPC分类号： C12N9/10 ,  C12P19/38

    CPC分类号： C12N9/1077 ,  C12P19/38

    摘要： The present invention relates to a recombinant expression vector comprising: a) the sequence encoding a purine nucleoside phosphorylase (PNPase, E. C. 2.4.2.1), b) the sequence encoding a uridine phosphorylase (UPase, E. C. 2.4.2.3), c) or both; each of the sequences operably linked to one or more control sequences that direct the production of said phosphorylases in a suitable expression host; said sequences originating from the Archaea Thermoprotei class, characterized in that the PNPase is from
    Sulfolobus solfataricus (SEQ ID NO. 7) and the UPase is from
    Aeropyrum pernix (SEQ ID NO. 8). In addition, the present invention relates to A transglycosylation method between a sugar-donating nucleoside and an acceptor base in the presence of phosphate ions, characterised in that said method comprises the use of a uridine phosphorylase (UPase) of
    Aeropyrum pernix (NC_000854.2), a purine nucleoside phosphorylase (PNPase) of
    Sulfolobus solfataricus (NC_002754.1), or a combination thereof.

    PROCESS FOR PRODUCING NUCLEOSIDE COMPOUND
    5.
    发明授权
    PROCESS FOR PRODUCING NUCLEOSIDE COMPOUND 有权
    一种用于生产核苷

    公开(公告)号：EP1338654B1

    公开(公告)日：2008-10-15

    申请号：EP01980986.2

    申请日：2001-11-06

    申请人： Mitsui Chemicals, Inc.

    发明人： ARAKI, Tadashi ,  IKEDA, Ichiro ,  TAKAHASHI, Katsuyuki ,  ITO, Kiyoshi ,  ASANO, Tamotsu ,  NIKUMARU, Seiya ,  NAKAMURA, Takeshi ,  ISHIBASHI, Hiroki ,  NAGAHARA, Kiyoteru ,  FUKUIRI, Yasushi

    IPC分类号： C12P19/38 ,  C12N15/09

    CPC分类号： C12P19/38

    摘要： In a process for producing a nucleoside compound by reacting pentose-1-phosphate with a nucleic acid base or a nucleic acid base analog in an aqueous reaction medium in the presence of a metal cation, the point and method of adding at least one of these reactants to the aqueous reaction medium are changed to thereby effectively produce the nucleotide compound at a high yield without causing an increase in viscosity of the liquid reaction mixture or the solidification thereof even in case of using the reactant at such an amount as causing the increase in viscosity of the liquid reaction mixture or the solidification thereof when employed as such. Thus, it becomes possible to provide a widely applicable process for producing a nucleoside compound which involves the step of reacting pentose-1-phosphate with a nucleic acid base or a nucleic acid base analog in the presence of a nucleoside phosphorylase activity and by which the conversion ratio of the nucleoside compound in the above reaction can be elevated.

    INDUSTRIALLY USEFUL MICROORGANISM
    8.
    发明公开
    INDUSTRIALLY USEFUL MICROORGANISM 审中-公开
    工业上有用的微生物

    公开(公告)号：EP1829965A1

    公开(公告)日：2007-09-05

    申请号：EP05809612.4

    申请日：2005-11-25

    申请人： KYOWA HAKKO KOGYO CO., LTD.

    发明人： MIZOGUCHI, Hiroshi ,  HARA, Kiyotaka ,  MORI, Hideo

    IPC分类号： C12N15/09 ,  C12N1/20 ,  C12N1/21 ,  C12P7/64 ,  C12P13/00 ,  C12P13/04 ,  C12P17/00 ,  C12P19/00 ,  C12P19/30 ,  C12P19/38 ,  C12P21/02

    CPC分类号： C12P13/08 ,  C12N1/20 ,  C12R1/185

    摘要： According to the present invention, the E . coli mutant strain having chromosomal DNA that is at least 470 kbp shorter than that of a wild-type E . coli strain exhibits such a property that the number of the cells after a certain period of culture is greater than a wild-type strain. Thus, by culturing the mutant strain in a medium so as to generate and accumulate a useful substance in the culture, a useful substance such as proteins, peptides, amino acids, nucleic acids, vitamins, saccharides, organic acids, and lipids can be efficiently produced.

    摘要翻译： 根据本发明，具有比野生型大肠杆菌菌株短至少470kbp的染色体DNA的大肠杆菌突变株显示出这样的性质，即培养一段时间后的细胞数目是 大于野生型菌株。 因此，通过在培养基中培养突变菌株以在培养物中产生并积累有用物质，可以有效地使蛋白质，肽，氨基酸，核酸，维生素，糖类，有机酸和脂质等有用物质 产生的。