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公开(公告)号：US10301563B2
公开(公告)日：2019-05-28
申请号：US15179469
申请日：2016-06-10
发明人： Rowena Justo Torres-Ordonez , Melanie Kuberka , Peter Placzek , Leslie R. Wolf , James J. Baustian
摘要： Gasoline blends and methods for producing gasoline blends containing high concentrations of a butanol isomer and having good cold start and warm-up driveability characteristics are disclosed.
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公开(公告)号：US10174345B2
公开(公告)日：2019-01-08
申请号：US15335628
申请日：2016-10-27
发明人： Yougen Li , Jessica McElvain , Steven Cary Rothman
摘要： Polypeptides having ketol-acid reductoisomerase activity are provided. Also disclosed are recombinant host cells comprising isobutanol biosynthetic pathways employing such polypeptides. Methods for producing isobutanol employing host cells comprising the polypeptides having ketol-acid reductoisomerase activity are also disclosed.
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公开(公告)号：US09944954B2
公开(公告)日：2018-04-17
申请号：US15228770
申请日：2016-08-04
发明人： Ritu Bhalla , Gopal K. Chotani , Michael Dauner , Mark J. Nelson , Daniel P. O'Keefe , Caroline M. Peres , Jahnavi Chandra Prasad , Jean-Francois Tomb
CPC分类号： C12P7/16 , C12N9/0006 , C12N9/0008 , C12N15/52 , C12Y101/01008 , C12Y101/01094 , C12Y101/05003 , C12Y102/01012 , Y02E50/10 , Y02P20/52
摘要： Provided herein are glycerol-3-phosphate dehydrogenase (GPD) enzymes with increased KM for NADH and GPD enzymes with substantially the same affinity for NADH and NADPH and/or are feedback inhibited by glycerol-3-phosphate. Also provided herein are recombinant microorganisms comprising a heterologous gene encoding GPD and a deletion or disruption in an endogenous gene encoding GPD. Also provided are recombinant microorganisms comprising a heterologous gene encoding GPD and a butanol biosynthetic pathway. Further provided are methods of producing butanol comprising providing the recombinant microorganisms described herein and contacting the recombinant microorganism with at least one fermentable carbon substrate under conditions wherein butanol is produced.
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公开(公告)号：US20170226497A1
公开(公告)日：2017-08-10
申请号：US15406876
申请日：2017-01-16
发明人： Kristen J. Kelly , Rick W. YE
CPC分类号： C12N9/88 , B01D11/04 , C12N15/52 , C12P7/16 , C12P7/40 , C12Y101/01001 , C12Y101/01086 , C12Y202/01006 , C12Y402/01009 , Y02E50/10 , Y02P20/52
摘要： Methods of screening for dihydroxy-acid dehydratase (DHAD) variants that display increased DHAD activity are disclosed, along with DHAD variants identified by these methods. Such enzymes can result in increased production of compounds from DHAD requiring biosynthetic pathways. Also disclosed are isolated nucleic acids encoding the DHAD variants, recombinant host cells comprising the isolated nucleic acid molecules, and methods of producing butanol.
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公开(公告)号：US20170145445A1
公开(公告)日：2017-05-25
申请号：US15293599
申请日：2016-10-14
发明人： Stephane Francois Bazzana , James Timothy Cronin , Michael Charles Grady , David J. Lowe , Brian Michael Roesch , Joseph J. Zaher , Raymond Richard Zolandz
CPC分类号： C12P7/16 , C11B1/025 , C11B1/10 , C12M21/12 , C12M33/00 , C12M41/26 , C12M41/48 , C12M43/02 , C12M47/10 , C12P7/10 , C12P7/14 , C12P2203/00 , Y02E50/16 , Y02E50/17
摘要： The present invention relates to the fermentative production of alcohols including ethanol and butanol, and processes for improving alcohol fermentation employing in situ product removal methods.
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公开(公告)号：US09517985B2
公开(公告)日：2016-12-13
申请号：US14772705
申请日：2014-03-14
发明人： Thomas Basham , Leslie William Bolton , Ian David Dobson , Sarah Richardson Hanson , Aidan Hurley , Karen Kustedjo , Andrew Richard Lucy , Liang Song
摘要： The invention relates to a method for producing butanol through microbial fermentation, in which the butanol product is removed by extraction into a water immiscible organic extractant during the fermentation. The invention also relates to a method for producing butanol through microbial fermentation, in which the butanol product is removed during the fermentation by extraction into a water-immiscible extractant composition. The invention further relates to compositions comprising a solution of butanol in a water immiscible organic extractant composition.
摘要翻译： 本发明涉及通过微生物发酵生产丁醇的方法，其中通过在发酵期间通过萃取到与水不混溶的有机萃取剂中除去丁醇产物。 本发明还涉及通过微生物发酵生产丁醇的方法，其中在发酵期间通过萃取到与水不混溶的萃取剂组合物中除去丁醇产物。 本发明还涉及包含丁醇在水不混溶的有机萃取剂组合物中的溶液的组合物。
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7.发明申请Fermentive Production of Isobutanol Using Highly Effective Ketol-Acid Reductoisomerase Enzymes 审中-公开使用高效酮酯酸重排酶酶的异丁醇发酵生产公开(公告)号：US20160319307A1
公开(公告)日：2016-11-03
申请号：US15209841
申请日：2016-07-14
CPC分类号： C12P7/16 , C12N9/0006 , C12N9/88 , C12N15/52 , C12P7/42 , C12Y101/01008 , C12Y101/01086 , C12Y401/01001 , C12Y401/01072 , C12Y401/02009 , C12Y402/01009 , Y02E50/10 , Y02P20/52
摘要： Ketol-acid reductoisomerase enzymes have been identified that provide high effectiveness in vivo as a step in an isobutanol biosynthetic pathway in bacteria and in yeast. These KARIs are members of a clade identified through molecular phylogenetic analysis called the SLSL Clade.
摘要翻译： 已经鉴定了酮醇酸还原异构酶，其在细菌和酵母中作为异丁醇生物合成途径的步骤在体内提供高效力。 这些KARI是通过称为SLSL分子的分子系统发育分析鉴定的进化枝的成员。
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8.发明申请Recombinant Yeast Host Cell With Fe-S Cluster Proteins And Methods Of Using Thereof 审中-公开具有Fe-S簇蛋白的重组酵母宿主细胞及其使用方法公开(公告)号：US20160222370A1
公开(公告)日：2016-08-04
申请号：US15016759
申请日：2016-02-05
发明人： Larry Cameron ANTHONY , Dennis Flint , Wonchul Suh , Rick W. Ye , Steven Cary Rothman , Jean-Francois Tomb
摘要： Yeast strains were engineered that have increased activity of heterologous proteins that require binding of an Fe—S cluster for their activity. The yeast strains have reduced activity of an endogenous Fe—S protein. Activities of heterologous bacterial, fungal or plant 2Fe-2S dihydroxy-acid dehydratases and Fe—S propanediol dehydratase reactivase were increased for increased production of products made using biosynthetic pathways including these enzymes, such as valine, isoleucine, leucine, pantothenic acid (vitamin B5), isobutanol, 2-butanone and 2-butanol.
摘要翻译： 工程化的酵母菌株具有增加Fe-S簇结合活性的异源蛋白质的活性。 酵母菌株内源性Fe-S蛋白的活性降低。 增加异源细菌，真菌或植物2Fe-2S二羟基酸脱水酶和Fe-S丙二醇脱水酶再活化酶的活性，以增加使用包括这些酶的生物合成途径（如缬氨酸，异亮氨酸，亮氨酸，泛酸（维生素B5 ），异丁醇，2-丁酮和2-丁醇。
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9.发明授权公开(公告)号：US09404117B2
公开(公告)日：2016-08-02
申请号：US14848792
申请日：2015-09-09
CPC分类号： C12P7/16 , C12N9/1025 , C12N9/1029 , C12N9/1096 , C12N9/88 , C12N15/81 , C12Y203/03013 , C12Y206/01042 , C12Y401/01001 , C12Y403/01019 , Y02E50/10 , Y02P20/52
摘要： Yeast cells with reduced activity of certain enzymes involved in branched chain amino acid biosynthesis in yeast mitochondria are described. Target enzymes include threonine deaminase, isopropylmalate synthase, and optionally branched chain amino acid transaminase.
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公开(公告)号：US09388392B2
公开(公告)日：2016-07-12
申请号：US14853108
申请日：2015-09-14
发明人： Sridhar Govindarajan , Yougen Li , Der-Ing Liao , Daniel P. O'Keefe , Jeremy Stephen Minshull , Steven Cary Rothman , Alexander Vincent Tobias
CPC分类号： C12N9/0006 , C12P7/16 , C12P7/42 , C12P7/44 , C12P13/02 , C12P13/06 , C12P13/08 , C12Y101/01086 , Y02E50/10 , Y02P20/52
摘要： Provided herein are polypeptides having ketol-acid reductoisomerase activity as well as microbial host cells comprising such polypeptides. Polypeptides provided herein may be used in biosynthetic pathways, including, but not limited to, isobutanol biosynthetic pathways.
摘要翻译： 本文提供具有酮酸酸还原异构酶活性的多肽以及包含这些多肽的微生物宿主细胞。 本文提供的多肽可用于生物合成途径，包括但不限于异丁醇生物合成途径。
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