-
1.发明授权公开(公告)号：US09459247B2
公开(公告)日：2016-10-04
申请号：US13075160
申请日：2011-03-29
Applicant: Huan-Chang Lin , Hsin-Hung Lin , Cai-Yu Kao , Alice L Yu , Wen-Ping Peng , Chung-Hsuan Chen
Inventor： Huan-Chang Lin , Hsin-Hung Lin , Cai-Yu Kao , Alice L Yu , Wen-Ping Peng , Chung-Hsuan Chen
IPC: A61K45/06 , C12Q1/70 , G01N33/50 , G01N33/569 , G01N33/68
CPC classification number: G01N33/5091 , G01N33/5008 , G01N33/569 , G01N33/6848
Abstract: Methods for detecting the presence of nanoparticles or microparticles by cell mass spectrometry (CMS) are provided. CMS methods are provided for determining the number of nanoparticles or microparticles in each cell. Nanoparticles whose intracellular concentration can be determines by the CMS methods of the invention include polymeric nanoparticles (NPs), liposomes, viral-based NPs, carbon nanotubes, diamond NPs, polymeric micelles, nanocarriers, liposomes, and viral nanoparticles. Determination of the efficiency of drug delivery and intracellular titer of pathogens according to the invention is disclosed. Methods for determining intracellular uptake of virus particles are provided.
Abstract translation: 提供了通过细胞质谱法（CMS）检测纳米颗粒或微粒的存在的方法。 提供CMS方法用于确定每个细胞中纳米颗粒或微粒的数量。 通过本发明的CMS方法可以确定其细胞内浓度的纳米颗粒包括聚合物纳米颗粒（NP），脂质体，基于病毒的NP，碳纳米管，金刚石NP，聚合胶束，纳米载体，脂质体和病毒纳米颗粒。 公开了根据本发明的药物递送效率和病原体细胞内滴度的测定。 提供了确定病毒颗粒的细胞内吸收的方法。
-
公开(公告)号：US09321818B2
公开(公告)日：2016-04-26
申请号：US12703102
申请日：2010-02-09
Applicant: Chung-Hsuan Chen , Wei-Chao Chang , Michael Hsiao , Ming-Shyan Huang , Chih-Jen Yang
Inventor： Chung-Hsuan Chen , Wei-Chao Chang , Michael Hsiao , Ming-Shyan Huang , Chih-Jen Yang
IPC: C12Q1/00 , C07K14/47 , A61K31/7088 , A61K33/24 , A61K38/04 , A61K45/06 , G01N33/574 , G01N30/72 , G01N33/68
CPC classification number: C07K14/4723 , A61K31/7088 , A61K33/24 , A61K38/04 , A61K45/06 , G01N30/72 , G01N33/57423 , G01N33/57488 , G01N33/6851 , A61K2300/00
Abstract: Methods for diagnosis of non-small cell lung cancers by detection of endogenous peptides in exhaled breath condensate (EBC) are provided. Diagnostic peptides derived from dermcidin (DC) are provided. A specific dermcidin-derived peptide E-R11, having the sequence ENAGEDPGLAR (SEQ ID NO:2), is provided. E-R11 peptide levels in EBC, as measured by mass spectrometry (MS), are highly diagnostic of non-small cell lung cancers. A method for inhibiting growth of lung cancer cells by inhibiting DCD expression by RNA interference also is provided.
Abstract translation: 提供了通过检测呼出气体浓缩物（EBC）中的内源肽来诊断非小细胞肺癌的方法。 提供衍生自皮肤霉素（DC）的诊断肽。 提供具有序列ENAGEDPGLAR（SEQ ID NO：2）的具体的皮肤霉素衍生肽E-R11。 通过质谱（MS）测量，EBC中的E-R11肽水平是非小细胞肺癌的高度诊断。 还提供了通过RNA干扰抑制DCD表达来抑制肺癌细胞生长的方法。
-
3.发明授权公开(公告)号：US08963075B2
公开(公告)日：2015-02-24
申请号：US12314520
申请日：2008-12-11
Applicant: Chung Hsuan Chen , Wen-Ping Peng , Ming Lee Chu , Huan Cheng Chang , Huan-Chang Lin
Inventor： Chung Hsuan Chen , Wen-Ping Peng , Ming Lee Chu , Huan Cheng Chang , Huan-Chang Lin
CPC classification number: H01J49/4265 , H01J49/0077 , H01J49/025
Abstract: A novel system and method for charge-monitoring mass spectrometry is provided. The mass spectrometer can be used to measure the mass of one or more analytes having masses in the range of about a few Daltons to more than about 1015 Daltons. The invention can be used for rapid mass distribution measurements. For example, the system and method can be used to distinguish cancer cells from normal cells when their mass distributions are different.
Abstract translation: 提供了一种电荷监测质谱的新型系统和方法。 质谱仪可用于测量质量在约几道尔顿至大于约1015道尔顿范围内的一种或多种分析物的质量。 本发明可用于快速质量分布测量。 例如，当它们的质量分布不同时，系统和方法可以用于区分癌细胞与正常细胞。
-
公开(公告)号：US08507846B2
公开(公告)日：2013-08-13
申请号：US13566352
申请日：2012-08-03
Applicant: Chung-Hsuan Chen , Jung-Lee Lin , Ming-Lee Chu
Inventor： Chung-Hsuan Chen , Jung-Lee Lin , Ming-Lee Chu
CPC classification number: H01J49/4285 , H01J49/0031 , H01J49/424
Abstract: An ion trap mass spectrometer and methods for obtaining a mass spectrum of ions by step scanning the driving frequency in frequency increments over a bandwidth, wherein for each step a specific frequency is held for a fixed number of complete cycles, wherein each specific frequency is changed continuously to the frequency in the next step, and wherein each specific frequency in each step starts at phase zero position.
Abstract translation: 一种离子阱质谱仪和用于通过在带宽上以频率增量扫描驱动频率来获得离子质谱的方法，其中对于每个步骤，特定频率保持固定数量的完整循环，其中每个特定频率被改变 在下一步骤中连续地进入频率，并且其中每个步骤中的每个特定频率从相位零位置开始。
-
公开(公告)号：US20130032709A1
公开(公告)日：2013-02-07
申请号：US13566352
申请日：2012-08-03
Applicant: Chung-Hsuan CHEN , Jung-Lee LIN , Ming-Lee CHU
Inventor： Chung-Hsuan CHEN , Jung-Lee LIN , Ming-Lee CHU
CPC classification number: H01J49/4285 , H01J49/0031 , H01J49/424
Abstract: An ion trap mass spectrometer and methods for obtaining a mass spectrum of ions by step scanning the driving frequency in frequency increments over a bandwidth, wherein for each step a specific frequency is held for a fixed number of complete cycles, wherein each specific frequency is changed continuously to the frequency in the next step, and wherein each specific frequency in each step starts at phase zero position.
Abstract translation: 一种离子阱质谱仪和用于通过在带宽上以频率增量扫描驱动频率来获得离子质谱的方法，其中对于每个步骤，特定频率保持固定数量的完整循环，其中每个特定频率被改变 在下一步骤中连续地进入频率，并且其中每个步骤中的每个特定频率从相位零位置开始。
-
Patent Agency Ranking
公开(公告)号：US20130001415A1
公开(公告)日：2013-01-03
申请号：US13534449
申请日：2012-06-27
Applicant: Chung-Hsuan CHEN , I-Chung LU , Ming-Lee CHU
Inventor： Chung-Hsuan CHEN , I-Chung LU , Ming-Lee CHU
CPC classification number: H01J49/4225 , H01J49/429
Abstract: An ion trap mass spectrometer and methods for obtaining a mass spectrum of ions by scanning an RF frequency applied to the linear ion trap for mass selective ejection of the ions by using two power amplifiers to apply opposite phases of the RF to x and y electrodes.
Abstract translation: 一种离子阱质谱仪和通过扫描施加到线性离子阱的RF频率来获得离子的质谱的方法，用于通过使用两个功率放大器将RF的相反相施加到x和y电极来质量选择性地喷射离子。
-
公开(公告)号：US20120126112A1
公开(公告)日：2012-05-24
申请号：US13365105
申请日：2012-02-02
Applicant: Chung Hsuan Chen , Jung-Lee Lin , Nien-Yeen Hsu , Yi-Sheng Wang
Inventor： Chung Hsuan Chen , Jung-Lee Lin , Nien-Yeen Hsu , Yi-Sheng Wang
CPC classification number: H05H5/047 , H01J49/06 , H01J49/40 , H01J49/403
Abstract: A novel system and methods for accelerating analytes including, without limitation, molecular ions, biomolecules, polymers, nano- and microparticles, is provided. The invention can be useful for increasing detection sensitivity in applications such as mass spectrometry, performing collision-induced dissociation molecular structure analysis, and probing surfaces and samples using accelerated analyte.
Abstract translation: 提供了一种用于加速分析物的新系统和方法，包括但不限于分子离子，生物分子，聚合物，纳米和微粒。 本发明可用于在诸如质谱法，进行碰撞诱导的解离分子结构分析和使用加速分析物的探测表面和样品等应用中提高检测灵敏度。
-
8.发明申请QUANTITATIVE MEASUREMENT OF NANO/MICRO PARTICLE ENDOCYTOSIS WITH CELL MASS SPECTROMETRY 审中-公开纳米/微粒子内毒素与细胞质谱的定量测量公开(公告)号：US20110236882A1
公开(公告)日：2011-09-29
申请号：US13075160
申请日：2011-03-29
Applicant: Huan-Chang LIN , Hsin-Hung LIN , Cai-Yu KAO , Alice L. Yu , Wen-Ping PENG , Chung-Hsuan Chen
Inventor： Huan-Chang LIN , Hsin-Hung LIN , Cai-Yu KAO , Alice L. Yu , Wen-Ping PENG , Chung-Hsuan Chen
CPC classification number: G01N33/5091 , G01N33/5008 , G01N33/569 , G01N33/6848
Abstract: Methods for detecting the presence of nanoparticles or microparticles by cell mass spectrometry (CMS) are provided. CMS methods are provided for determining the number of nanoparticles or microparticles in each cell. Nanoparticles whose intracellular concentration can be determines by the CMS methods of the invention include polymeric nanoparticles (NPs), liposomes, viral-based NPs, carbon nanotubes, diamond NPs, polymeric micelles, nanocarriers, liposomes, and viral nanoparticles. Determination of the efficiency of drug delivery and intracellular titer of pathogens according to the invention is disclosed. Methods for determining intracellular uptake of virus particles are provided.
Abstract translation: 提供了通过细胞质谱法（CMS）检测纳米颗粒或微粒的存在的方法。 提供CMS方法用于确定每个细胞中纳米颗粒或微粒的数量。 通过本发明的CMS方法可以确定其细胞内浓度的纳米颗粒包括聚合物纳米颗粒（NP），脂质体，基于病毒的NP，碳纳米管，金刚石NP，聚合胶束，纳米载体，脂质体和病毒纳米颗粒。 公开了根据本发明的药物递送效率和病原体细胞内滴度的测定。 提供了确定病毒颗粒的细胞内吸收的方法。
-
公开(公告)号：US20100181474A1
公开(公告)日：2010-07-22
申请号：US12689506
申请日：2010-01-19
Applicant: Yi-Sheng Wang , Chung-Hsuan Chen
Inventor： Yi-Sheng Wang , Chung-Hsuan Chen
CPC classification number: H01J49/40 , H01J49/0095
Abstract: An angled dual-polarity mass spectrometer includes a dual-polarity ion generator, a first mass analyzer, and a second mass analyzer. The dual-polarity ion generator includes an ion source to generate positive ions and negative ions from a sample, and electrodes to generate electric fields for guiding the negative ions into a beam of negative ions and guiding the positive ions into a beam of positive ions. The first mass analyzer can analyze the negative ions, and the second mass analyzer can analyze the positive ions. The central axes of the first and the second mass analyzers are at an angle between 0 to 179 degrees.
Abstract translation: 角度双极性质谱仪包括双极性离子发生器，第一质量分析器和第二质量分析器。 双极性离子发生器包括从样品产生正离子和负离子的离子源，以及用于产生电场的电极，用于将负离子引导到负离子束中并将正离子引导到正离子束中。 第一个质谱仪可以分析负离子，第二个质谱仪可以分析正离子。 第一和第二质量分析器的中心轴线在0至179度之间的角度。
-
公开(公告)号：US20060024815A1
公开(公告)日：2006-02-02
申请号：US10903569
申请日：2004-07-30
Applicant: Steve Allman , Hannah Farquar , Chung-Hsuan Chen
Inventor： Steve Allman , Hannah Farquar , Chung-Hsuan Chen
IPC: C12M1/34
CPC classification number: G01N21/6452 , G01N21/253 , G01N33/54373
Abstract: A high sensitivity array-based detection system includes a plurality of probes immobilized onto a plurality of locations provided on a surface of a patterned array, such as a microarray. At least one target selectively binds to at least one of the plurality of probes when disposed proximate thereto under appropriate conditions, such as temperature and pH. The target includes at least one bound particle. The bound particle includes a plurality of label molecules. Alternatively, the target can be immobilized to the surface of the array, such as from a sample suspected of including the target. An excitation light source provides excitation light incident to one or more locations on the patterned array. A photodetector detects emanated signals from the patterned array. The photodetector can be a commercially available digital camera.
Abstract translation: 基于高灵敏度的基于阵列的检测系统包括固定在设置在诸如微阵列的图案化阵列的表面上的多个位置上的多个探针。 当在适当的条件下，例如温度和pH值时，至少一个靶选择性地结合至多个探针中的至少一个。 目标包括至少一个结合粒子。 结合粒子包括多个标记分子。 或者，靶可以被固定到阵列的表面，例如从怀疑包括靶的样品中。 激发光源提供入射到图案化阵列上的一个或多个位置的激发光。 光电检测器检测来自图案化阵列的发射信号。 光电检测器可以是市售的数码相机。
-
-
-
-
-
-
-
-
-
Search Results
33
records
(took 0.025 seconds)
