公开(公告)号：US10465153B2

公开(公告)日：2019-11-05

申请号：US15037539

申请日：2014-11-26

Applicant: GE Healthcare BioProcess R&D AB

Inventor： Jean-Luc Maloisel ,  Tobias Soderman ,  Ola Lind

IPC: C12H1/056 ,  B01D15/00 ,  B01J20/32 ,  B01J20/28 ,  C08F251/00

Abstract: The invention discloses a separation matrix with a porous solid support and a plurality of polyvinylpyrrolidone (PVP) polymer chains covalently attached to the solid support. The polyvinylpyrrolidone polymer chains are either vinylpyrrolidone homopolymer chains or copolymer chains which comprise at least 70 mol % vinylpyrrolidone monomer residues and less than 2 mol % negatively charged monomer residues.

公开(公告)号：US20190324001A1

公开(公告)日：2019-10-24

申请号：US16459525

申请日：2019-07-01

Applicant: GE HEALTHCARE BIOPROCESS R&D AB

Inventor： Klaus Gebauer

IPC: G01N30/60 ,  B01D15/18 ,  B01D15/22

Abstract: The present invention relates to axial flow chromatography columns, methods for separating one or more analytes in a liquid by the use of such columns, and systems employing such columns. The column comprises a first port and a second port the first port and said second port being at essentially the same level or elevation above the level of the bed space on the chromatography column.

公开(公告)号：US20190308168A1

公开(公告)日：2019-10-10

申请号：US16443600

申请日：2019-06-17

Applicant: GE Healthcare BioProcess R&D AB

Inventor： Martin HALL ,  Sture LARSSON ,  Andreas MURANYI ,  Gustav RODRIGO ,  Jinyu ZOU ,  Per-Mikael ABERG

IPC: B01J20/24 ,  B01D15/38 ,  B01J20/32 ,  B01J20/285 ,  B01J20/286 ,  C07K17/10 ,  C07K17/00 ,  C07K16/00 ,  C07K14/31 ,  C07K1/22 ,  B01J20/28 ,  B01J20/289

Abstract: The present invention relates to a chromatography ligand, which comprises Domain C from Staphylococcus protein A (SpA), or a functional fragment or variant thereof. The chromatography ligand presents an advantageous capability of withstanding harsh cleaning in place (CIP) conditions, and is capable of binding Fab fragments of antibodies. The ligand may be provided with a terminal coupling group, such as arginine or cysteine, to facilitate its coupling to an insoluble carrier such as beads or a membrane. The invention also relates to a process of using the ligand in isolation of antibodies, and to a purification protocol which may include washing steps and/or regeneration with alkali.

公开(公告)号：US20190224588A1

公开(公告)日：2019-07-25

申请号：US16306619

申请日：2017-06-19

Applicant: GE HEALTHCARE BIOPROCESS R&D AB

Inventor： Klaus Gebauer ,  Spyridon Gerontas ,  Jamil Shanagar

IPC: B01D15/20 ,  B01D15/18 ,  G01N30/46 ,  G01N30/56 ,  G01N30/60 ,  A61L2/08

Abstract: The invention discloses a method for packing a plurality of uniform chromatography columns, comprising the steps of: a) providing a plurality of chromatography columns; b) providing a plurality of chromatography resin aliquots; c) packing the chromatography resin aliquots in the chromatography columns to provide a plurality of packed chromatography columns; and d) subjecting the packed chromatography columns to repeated mechanical impacts to provide a plurality of uniform chromatography columns.

公开(公告)号：US10308690B2

公开(公告)日：2019-06-04

申请号：US14961164

申请日：2015-12-07

Applicant: GE HEALTHCARE BIOPROCESS R&D AB

Inventor： Gustav Rodrigo ,  Mats Ander ,  Göran Baurén ,  Tomas Björkman

IPC: C07K14/31 ,  B01J20/32 ,  B01D15/38 ,  C07K1/22 ,  B01J20/286 ,  C07K14/745 ,  C07K16/00

Abstract: The invention discloses a polypeptide with improved alkaline stability, which polypeptide comprises a mutant of a B or C domain of Staphylococcus Protein A (SpA), as specified by SEQ ID NO 1 or SEQ ID NO 2, or of Protein Z, as specified by SEQ ID NO 3, comprising at least the mutation wherein the glutamine residue at position 9 has been mutated to a tryptophan, leucine, glutamic acid, valine or lysine. The invention also discloses multimers of the polypeptide, as well as separation matrices comprising the multimers or polypeptides.

公开(公告)号：US20190154639A1

公开(公告)日：2019-05-23

申请号：US16067628

申请日：2017-01-06

Applicant: GE HEALTHCARE BIOPROCESS R&D AB

Inventor： Klaus Gebauer ,  Dan Hermansson

IPC: G01N30/60 ,  B01D15/22 ,  G01N30/56 ,  B01D15/20

Abstract: A method for packing a chromatography column with chromatography media, comprising the steps of: providing a system comprising a column tube (3,19,21) having a closed first end (5) comprising an inlet/outlet (7), a media inlet (15) adjacent a second end of the column tube and an adaptor (9) positioned inside the column tube initially adjacent the second end of the column tube for sliding and sealing contact with an inner face of the column tube, the column tube and adaptor arranged initially such that they define an internal volume and such that the media inlet is in fluid connection with the internal volume; connecting a media slurry source to the media inlet; at least partially filling the internal volume with media slurry via the media inlet; forcing the adaptor towards the first end of the column tube to reduce the internal volume such that the media inlet is no longer in fluid connection with the reduced internal volume.

公开(公告)号：US20190144511A1

公开(公告)日：2019-05-16

申请号：US16252015

申请日：2019-01-18

Applicant: GE HEALTHCARE BIOPROCESS R&D AB

Inventor： Gustav Rodrigo ,  Mats Ander ,  Tomas Bjorkman

IPC: C07K14/195 ,  C07K16/00 ,  C07K1/22

Abstract: A kappa light chain-binding polypeptide comprising or consisting essentially of one or more mutated binding domains of Peptostreptococcus Protein L.

公开(公告)号：US20190111419A1

公开(公告)日：2019-04-18

申请号：US16090609

申请日：2017-03-30

Applicant: GE HEALTHCARE BIOPROCESS R&D AB

Inventor： Jean-Luc Maloisel ,  Tobias Söderman ,  Ola Lind ,  Ida Eklind

IPC: B01J47/02 ,  B01J39/07 ,  B01J39/19 ,  B01J39/26 ,  B01J41/07 ,  B01J41/13 ,  B01J41/20 ,  C07K1/18 ,  B01D15/36 ,  B01D15/38 ,  B01D15/32

Abstract: The invention discloses a separation matrix for purification of biomacromolecules, comprising a plurality of particles (1) having a core region (2) and a shell region (3), wherein: a) said shell region is accessible to a target biomacromolecule; b) said core region is less accessible to the target biomacromolecule than the shell region; and c) the core region comprises a grafted polymer comprising residues of at least one polymerizable monomer.

公开(公告)号：US20180356375A1

公开(公告)日：2018-12-13

申请号：US16103149

申请日：2018-08-14

Applicant: GE Healthcare BioProcess R&D AB

Inventor： Klaus Gebauer

IPC: G01N30/60 ,  B01D15/22 ,  B01D15/18

CPC classification number: G01N30/6004 ,  B01D15/18 ,  B01D15/22 ,  G01N30/6017 ,  G01N30/6021 ,  G01N30/6052 ,  G01N30/606

Abstract: The present invention relates to axial flow chromatography columns, methods for separating one or more analytes in a liquid by the use of such columns, and systems employing such columns. The column comprises a first port and a second port, the first port and said second port being at essentially the same level or elevation above the level of the bed space on the chromatography column.

公开(公告)号：US10112980B2

公开(公告)日：2018-10-30

申请号：US15598685

申请日：2017-05-18

Applicant: GE Healthcare BioProcess R&D AB

Inventor： Mats Ander ,  Goran Bauren ,  Tomas Bjorkman ,  Gustav Rodrigo

IPC: C07K14/31 ,  C07K14/745 ,  C07K16/00 ,  C07K1/22 ,  B01D15/38 ,  B01J20/32 ,  B01J20/286

Abstract: The invention discloses a polypeptide with improved alkaline stability, which polypeptide comprises a mutant of a B or C domain of Staphylococcus Protein A (SpA), as specified by SEQ ID NO 1 or SEQ ID NO 2, or of Protein Z, as specified by SEQ ID NO 3, wherein at least the glutamic acid residue at position 15 has been mutated to an amino acid other than asparagine. The invention also discloses multimers of said polypeptide, as well as separation matrices comprising the multimers or polypeptides.