公开(公告)号：US12226561B2

公开(公告)日：2025-02-18

申请号：US17671353

申请日：2022-02-14

Applicant: Life Technologies Corporation ,  Life Technologies AS

Inventor： Lars Norderhaug ,  Angel Varela-Rohena ,  Nephi D. Jones

IPC: A61M1/36 ,  B01L3/00 ,  B03C1/01 ,  B03C1/28 ,  B03C1/30 ,  C07K16/28 ,  C12M1/00 ,  C12M1/12 ,  C12M1/26 ,  C12M3/00

Abstract: A magnetic particle separation system includes a magnetic field generating device having an upper surface with a receiving area formed thereon; and a magnetic field generating element disposed beneath the upper surface, the magnetic field generating element being configured to produce a magnetic field above the upper surface. A container assembly is disposed on the upper surface and includes: a flexible container having an outer wall with an interior surface that at least partially bounds an internal compartment, the outer wall having a front side and an opposing back side with the internal compartment disposed therebetween; a fluid inlet extending through the outer wall at the front side; a fluid outlet extending through the outer wall at the front side; and a first partition projecting into the internal compartment from the front side between the fluid inlet and the fluid outlet.

公开(公告)号：US20240392226A1

公开(公告)日：2024-11-28

申请号：US18248428

申请日：2021-10-12

Applicant: LIFE TECHNOLOGIES AS ,  LIFE TECHNOLOGIES CORPORATION ,  LIFE TECHNOLOGIES HOLDINGS PTE LIMITED

Inventor： Morten LUHR ,  Michael GORDON ,  Ming Tiong SIA ,  Kok Shyong CHONG ,  Woon Liang Terence SOH ,  Seng Leong LIM ,  Ida Caroline SCHROEDER ,  Katrine ALFSNES ,  Terry JianHui LIM ,  Marie BOSNES ,  Axl Alois NEURAUTER ,  Ketil Winther PEDERSEN ,  Autumn Day WILBUR ,  Sean ZIMMERMAN

IPC: C12M1/12 ,  C12M1/00 ,  G01N35/00

Abstract: A bead processing assembly for use in attaching magnetic beads to biological cells and other biological materials and/or separating magnetic beads from biological cells and other biological materials includes a base assembly having a housing, a support panel disposed on the housing and having a front face, a first pinch valve at least partially outwardly projecting from the front face of the support panel, and a first pump at least partially outwardly projecting from the front face of the support panel. A rocker assembly is supported on the base assembly and includes a mount assembly supported on the base assembly, a platform assembly pivotably secured to the mount assembly, and a rocker drive for repeatedly rocking the platform assembly relative to the mount assembly.

公开(公告)号：US20230167210A1

公开(公告)日：2023-06-01

申请号：US18101785

申请日：2023-01-26

Applicant: LIFE TECHNOLOGIES CORPORATION ,  LIFE TECHNOLOGIES AS

Inventor： Geir FONNUM ,  Grete Irene MODAHL ,  Nini Hofsloekken KJUS ,  Astrid Evenroed MOLTEBERG ,  Diem Thuy Thi TRAN ,  Jo AASERUD ,  M. Talha GOKMEN ,  Steven M. MENCHEN ,  Carl FULLER ,  Luisa ANDRUZZI ,  Wolfgang HINZ

IPC: C08F130/08 ,  C07F7/18 ,  C08F8/12 ,  C12Q1/686 ,  C08F2/20 ,  C08F2/26 ,  C08F230/08 ,  C08F6/24 ,  C08F222/38 ,  C08F8/32 ,  C08F8/34 ,  C12P19/34 ,  C12Q1/6874 ,  G01N27/414 ,  C08J9/20

CPC classification number: C08F130/08 ,  C07F7/1804 ,  C08F8/12 ,  C12Q1/686 ,  C07F7/188 ,  C08F2/20 ,  C08F2/26 ,  C08F230/085 ,  C08F6/24 ,  C08F222/385 ,  C08F8/32 ,  C08F8/34 ,  C12P19/34 ,  C12Q1/6874 ,  G01N27/4145 ,  C08J9/20 ,  Y10T428/2982 ,  C08F2800/20 ,  C08F220/603

Abstract: A method of forming a particle includes, in a disperse phase within an aqueous suspension, polymerizing a plurality of mer units of a hydrophilic monomer having a hydrophobic protection group, thereby forming a polymeric particle including a plurality of the hydrophobic protection groups. The method further includes converting the polymeric particle to a hydrophilic particle.

公开(公告)号：US20220275331A1

公开(公告)日：2022-09-01

申请号：US17578172

申请日：2022-01-18

Applicant: LIFE TECHNOLOGIES AS

Inventor： Hilde ALMAASBAK ,  Tanja AARVAK ,  Kerstin BERNSTROEM ,  Oystein AAMELLEM

IPC: C12N5/0783

Abstract: This invention relates, inter alia, to compositions of expanded T cell populations, methods for the expansion of T cell populations and methods for using such populations of cells. In some aspects, the invention relates to compositions and methods for the selective expansion of T cell subpopulations present in mixed T cell populations, as well as T cell subpopulations produced by methods for the invention.

公开(公告)号：US20190264017A1

公开(公告)日：2019-08-29

申请号：US16305372

申请日：2017-06-07

Applicant: LIFE TECHNOLOGIES AS ,  THERMO FISHER SCIENTIFIC GENEART GMBH

Inventor： Kristian ANDERSSON ,  Geir FONNUM ,  Korbinian HEIL

IPC: C08L25/14 ,  C08F212/08 ,  B01J19/00

Abstract: This invention relates to polymer particles for solid phase oligonucleotide synthesis. The oligonucleotide may be linked to the particle via a linker having an amide-oligoethyleneglycol-amine structure. The particles may be considered to act as a solid support during the oligonucleotide synthesis. Also disclosed are processes for preparing such polymer particles, compositions and systems comprising such particles, and uses thereof.

公开(公告)号：US10246533B2

公开(公告)日：2019-04-02

申请号：US14970192

申请日：2015-12-15

Applicant: LIFE TECHNOLOGIES CORPORATION ,  LIFE TECHNOLOGIES AS

Inventor： Geir Fonnum ,  Grete Irene Modahl ,  Nini Hofslokken Kjus ,  Astrid Evenroed Molteberg ,  Diem Tran ,  Jo Aaserud ,  M. Talha Gokmen ,  Steven M. Menchen ,  Carl Fuller ,  Luisa Andruzzi ,  Wolfgang Hinz

IPC: C08F130/08 ,  C08F6/24 ,  C07F7/18 ,  C08F2/20 ,  C08F2/26 ,  C08F220/58 ,  C08F230/08 ,  C08F222/38 ,  C08F8/32 ,  C08F8/34 ,  C12Q1/686 ,  C12P19/34 ,  C12Q1/6874 ,  G01N27/414 ,  C08J9/20 ,  C08F8/12

Abstract: A method of forming a particle includes, in a disperse phase within an aqueous suspension, polymerizing a plurality of monomer units of a hydrophilic monomer having a hydrophobic protection group, thereby forming a polymeric particle including a plurality of the hydrophobic protection groups. The method further includes converting the polymeric particle to a hydrophilic particle.

公开(公告)号：US20190062706A1

公开(公告)日：2019-02-28

申请号：US15771821

申请日：2016-10-27

Applicant: LIFE TECHNOLOGIES AS

Inventor： Hilde ALMAASBAK ,  Tanja AARVAK ,  Kerstin BERNSTROEM ,  Oystein AAMELLEM

IPC: C12N5/0783

Abstract: This invention relates, inter alia, to compositions of expanded T cell populations, methods for the expansion of T cell populations and methods for using such populations of cells. In some aspects, the invention relates to compositions and methods for the selective expansion of T cell subpopulations present in mixed T cell populations, as well as T cell subpopulations produced by methods for the invention.

公开(公告)号：US20180237595A1

公开(公告)日：2018-08-23

申请号：US15871863

申请日：2018-01-15

Applicant: Life Technologies AS ,  Life Technologies Corporation

Inventor： Geir FONNUM ,  Steven M. MENCHEN ,  M. Talha GOKMEN ,  Pontus LUNDBERG ,  Prasanna Krishnan THWAR ,  Alfred LUI ,  Lily LU ,  Wolfgang HINZ ,  Lene Husaboe ,  Elisabeth BREIVOLD ,  Astrid Evenroed MOLTEBERG ,  Synne LARSEN

IPC: C08J3/075 ,  C08F8/12

CPC classification number: C08J3/075 ,  C08F8/12 ,  C08F220/58 ,  C08F220/70 ,  C08F2230/085 ,  C08J2333/26 ,  C08F222/38

Abstract: A polymer substrate, such as a polymer particle, is formed from a carboxyl functional monomer. In an example, the carboxyl functional monomer has a protection group in place of the OH of the carboxyl group. Once the monomer is polymerized, such a protection group can be removed, providing a polymer network with carboxyl functional sites. Such sites can be used to attach other functionality to the polymer substrate.

公开(公告)号：US20180215852A1

公开(公告)日：2018-08-02

申请号：US15745620

申请日：2016-07-19

Applicant: LIFE TECHNOLOGIES AS

Inventor： Geir FONNUM ,  Nils LUNDBERG ,  Muhammed GOKMEN ,  Synne LARSEN ,  Wilhelm Glomm ,  Heidi Johnsen ,  Ruth SCHMID ,  Lars Kilaas

IPC: C08F265/10 ,  C08F120/54 ,  C08F265/06

CPC classification number: C08F265/10 ,  C08F120/54 ,  C08F265/06 ,  C08F220/56

Abstract: This invention relates to monodisperse cross-linked hydrogel polymer particles comprising a polymer formed from (a) a hydrophilic vinylic monomer; and (b) a crosslinker comprising at least two vinyl groups. The invention also relates to monodisperse seed particles with a Z-average diameter of from 100 nm to 1500 nm that comprise a plurality of non-crosslinked oligomers of poly N,N-dimethylacrylamide. Also provided are methods of forming the monodisperse cross-linked hydrogel polymer particles and monodisperse seed particles.

公开(公告)号：US09868826B2

公开(公告)日：2018-01-16

申请号：US15200533

申请日：2016-07-01

Applicant: LIFE TECHNOLOGIES CORPORATION ,  LIFE TECHNOLOGIES AS

Inventor： Geir Fonnum ,  Steven M. Menchen ,  Talha M. Gokmen ,  Pontus Lundberg ,  Prasanna Krishnan Thwar ,  Alfred Lui ,  Lily Lu ,  Wolfgang Hinz ,  Lene Husaboe ,  Elisabeth Breivold ,  Astrid Evenroed Molteberg ,  Synne Larsen

IPC: C08J3/075 ,  C08F220/58 ,  C08F8/12 ,  C08F220/70 ,  C08F230/08

CPC classification number: C08J3/075 ,  C08F8/12 ,  C08F220/58 ,  C08F220/70 ,  C08F2230/085 ,  C08J2333/26 ,  C08F222/38

Abstract: A polymer substrate, such as a polymer particle, is formed from a carboxyl functional monomer. In an example, the carboxyl functional monomer has a protection group in place of the OH of the carboxyl group. Once the monomer is polymerized, such a protection group can be removed, providing a polymer network with carboxyl functional sites. Such sites can be used to attach other functionality to the polymer substrate.