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Patent Agency Ranking
公开(公告)号：US20210023561A1
公开(公告)日：2021-01-28
申请号：US16922265
申请日：2020-07-07
Applicant: STOKES BIO LTD.
Inventor： Mark DAVIES , Tara DALTON , Kieran CURRAN
Abstract: A bridge (30) comprises a first inlet port (31) at the end of a capillary, a narrower second inlet port (32) which is an end of a capillary, an outlet port (33) which is an end of a capillary, and a chamber (34) for silicone oil. The oil is density-matched with the reactor droplets such that a neutrally buoyant environment is created within the chamber (34). The oil within the chamber is continuously replenished by the oil separating the reactor droplets. This causes the droplets to assume a stable capillary-suspended spherical form upon entering the chamber (34). The spherical shape grows until large enough to span the gap between the ports, forming an axisym metric liquid bridge. The introduction of a second droplet from the second inlet port (32) causes the formation of an unstable funicular bridge that quickly ruptures from the, finer, second inlet port (32), and the droplets combine at the liquid bridge (30). In another embodiment, a droplet (55) segments into smaller droplets which bridge the gap between the inlet and outlet ports.
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公开(公告)号：US20230295703A1
公开(公告)日：2023-09-21
申请号：US18164453
申请日：2023-02-03
Applicant: STOKES BIO LTD.
Inventor： Mark B. DAVIES , Tara DALTON
IPC: C12Q1/6851 , B01L3/00 , B01L7/00
CPC classification number: C12Q1/6851 , B01L3/502784 , B01L7/525 , B01L2300/0838 , B01L2300/0867 , B01L2400/0487
Abstract: A method comprises flowing a plurality of sample droplets in a continuous flow of a carrier fluid, immiscible with the sample droplets, such that the droplets are separated from each other by the carrier fluid, wherein an average number of copies of target nucleic acid contained in each droplet the plurality of sample droplets is one or fewer. The method may further comprise subjecting the droplets to thermal cycling sufficient to allow amplification of the target nucleic acid, and detecting one or more of the presence or absence of amplified target nucleic acid in the droplets.
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公开(公告)号：US11807902B2
公开(公告)日：2023-11-07
申请号：US16892488
申请日：2020-06-04
Applicant: STOKES BIO LTD.
Inventor： Mark Davies , Tara Dalton
CPC classification number: C12Q1/686 , B01F25/10 , B01F33/30 , B01L3/50273 , B01L3/502776 , B01L3/502784 , B01L7/525 , G01N35/08 , B01L2200/0636 , B01L2200/0673 , B01L2200/141 , B01L2300/087 , B01L2300/0816 , B01L2300/0861 , B01L2300/185 , B01L2400/0409 , B01L2400/0487 , G01N35/1095 , G01N2035/00514
Abstract: A microfluidic analysis system (1) performs polymerase chain reaction (PCR) analysis on a bio sample. In a centrifuge (6) the sample is separated into DNA and RNA constituents. The vortex is created by opposing flow of a silicon oil primary carrier fluid effecting circulation by viscous drag. The bio sample exits the centrifuge enveloped in the primary carrier fluid. This is pumped by a flow controller (7) to a thermal stage (9). The thermal stage (9) has a number of microfluidic devices (70) each having thermal zones (71, 72, 73) in which the bio sample is heated or cooled by heat conduction to/from a thermal carrier fluid and the primary carrier fluid. Thus, the carrier fluids envelope the sample, control its flowrate, and control its temperature without need for moving parts at the micro scale.
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公开(公告)号：US11772096B2
公开(公告)日：2023-10-03
申请号：US16922265
申请日：2020-07-07
Applicant: STOKES BIO LTD.
Inventor： Mark Davies , Tara Dalton , Kieran Curran
IPC: B01L3/00 , B01F13/00 , B01F15/02 , F17D1/08 , C12Q1/686 , B01F35/71 , B01F33/302 , B01F101/23 , B01L7/00
CPC classification number: B01L3/502784 , B01F33/3021 , B01F35/7172 , B01L3/502715 , B01L3/502746 , C12Q1/686 , F17D1/08 , B01F35/71 , B01F2101/23 , B01L3/5025 , B01L7/525 , B01L2200/0673 , B01L2300/0829 , B01L2300/0838 , B01L2400/0478 , B01L2400/0487 , B01L2400/0633 , B01L2400/082 , Y10T137/87676 , Y10T436/117497 , Y10T436/118339 , Y10T436/2575
Abstract: A system for processing a biological sample includes a substrate comprising a plurality of wells and a plurality of flow channels. The system further includes a flow control system comprising a manifold having a plurality of ports configured to fluidically couple to the plurality of wells, and one or more containment structures configured to contain carrier fluid and fluidically couple to the ports. The flow control system further includes a cradle configured to removably receive the substrate. The flow control system is configured to transmit pressure differential, via the manifold, to the plurality of wells so as to cause a plurality of sample volumes held by at least some wells of the plurality of wells to flow through respective flow channels and cause the carrier fluid to flow through the flow channels and form a plurality of droplets of the plurality of sample volumes separated by the carrier fluid.
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公开(公告)号：US20200283837A1
公开(公告)日：2020-09-10
申请号：US16812987
申请日：2020-03-09
Applicant: STOKES BIO LTD.
Inventor： Mark B. DAVIES , Tara DALTON
IPC: C12Q1/6851 , B01L3/00 , B01L7/00
Abstract: A method comprises flowing a plurality of sample droplets in a continuous flow of a carrier fluid, immiscible with the sample droplets, such that the droplets are separated from each other by the carrier fluid, wherein an average number of copies of target nucleic acid contained in each droplet the plurality of sample droplets is one or fewer. The method may further comprise subjecting the droplets to thermal cycling sufficient to allow amplification of the target nucleic acid, and detecting one or more of the presence or absence of amplified target nucleic acid in the droplets.
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