公开(公告)号：US20230355738A1

公开(公告)日：2023-11-09

申请号：US18138183

申请日：2023-04-24

Applicant: The Catholic University of America

Inventor： Venigalla B. RAO ,  Jingen ZHU

IPC: A61P37/04 ,  A61K39/12

CPC classification number: A61K39/12 ,  A61P37/04 ,  A61K2039/545

Abstract: A bacteriophage T4-based, multivalent/multicomponent, needle and adjuvant-free, mucosal vaccine by engineering spike trimers on capsid exterior and nucleocapsid protein in the interior is disclosed herein. Intranasal administration of this T4-COVID vaccine induces higher virus neutralization antibody titers against multiple variants, balanced Th1/Th2 antibody and cytokine responses, stronger CD4+ and CD8+ T cell immunity, and higher secretory IgA titers in sera and bronchoalveolar lavage with no effect on the gut microbiota, compared to vaccination of mice intramuscularly. The vaccine is stable at ambient temperature, induce apparent sterilizing immunity, and provide complete protection against original SARS-CoV-2 strain and its Delta variant with minimal lung histopathology. This mucosal vaccine is an excellent candidate for boosting immunity of immunized and/or as a second-generation vaccine for the unimmunized population. This needle-free platform could be used to develop effective vaccines against many other respiratory infectious pathogens including Flu and any future emerging epidemic and pandemic pathogens.

公开(公告)号：US11804712B2

公开(公告)日：2023-10-31

申请号：US17230201

申请日：2021-04-14

Applicant: THE CATHOLIC UNIVERSITY OF AMERICA

Inventor： Kevin F. Forbes

IPC: H02J3/00 ,  G05B17/02 ,  G06F16/27 ,  H02J3/38

CPC classification number: H02J3/003 ,  G05B17/02 ,  G06F16/27 ,  H02J3/004 ,  H02J3/381 ,  H02J2203/20 ,  H02J2300/24 ,  H02J2300/28

Abstract: Systems and methods for improving load energy forecasting in the presence of distributed energy resources in which a revised load forecast is calculated based on forecasted meteorological conditions data, forecasted wind and solar energy, forecasted load data, time data and time-series variables determined based on an analysis of the historical data. In exemplary embodiments, the revised load forecast is provided to energy management computer systems to enable appropriate levels of generation of conventional and renewable energy generation within the electric power grid.

公开(公告)号：US20220074069A1

公开(公告)日：2022-03-10

申请号：US17470105

申请日：2021-09-09

Applicant: The Catholic University of America

Inventor： XIAOLONG LUO ,  PIAO HU

IPC: C25D17/00 ,  C25D1/00 ,  C25D17/10

Abstract: The present disclosure relates to a device for and a method of interfacial electrofabricatio of freestanding biopolymer membrane. The present disclosure also relates to the freestanding biopolymer membrane fabricated using the method provided in the present disclosure and applications of the presently disclosed freestanding biopolymer membrane.

公开(公告)号：US20220033850A1

公开(公告)日：2022-02-03

申请号：US17359711

申请日：2021-06-28

Applicant: The Catholic University of America

Inventor： Venigalla B. RAO ,  Jingen ZHU

IPC: C12N15/86 ,  C12N7/00

Abstract: Described is an “artificial virus” (AV) programmed with biomolecules that can enter human cells and carry out precise human genome modification. The AVs comprise: at least one viral vector, such as bacteriophage T4; at least one therapeutic molecule, such as DNA, RNA, protein and their complex; and a lipid coating. Also described is a method of human genome modification, using such an AV, and a method of program such an AV.

公开(公告)号：US20200062645A1

公开(公告)日：2020-02-27

申请号：US16545012

申请日：2019-08-20

Applicant: The Catholic University of America

Inventor： Weiliang GONG ,  Hui XU ,  Werner LUTZE ,  Ian L. PEGG

IPC: C04B18/08 ,  C04B7/06 ,  C04B12/00 ,  C04B14/36 ,  C04B14/26 ,  C04B18/06 ,  C04B18/16

Abstract: An embodiment includes a Class C fly ash (CFA) cementitious composition with a controllable setting time comprising at least one Class C fly ash; at least one alkali hydroxide; at least one source of phosphate; and water. Alternate embodiments include a Class C fly ash (CFA) cementitious composition with a solid activator comprising at least one Class C fly ash; at least one alkali carbonate; at least one source of phosphate; and water.

公开(公告)号：US20200031717A1

公开(公告)日：2020-01-30

申请号：US16517802

申请日：2019-07-22

Applicant: The Catholic University of America

Inventor： Weiliang Gong ,  Hui Xu ,  Werner Lutze ,  Ian L. Pegg

IPC: C04B28/08 ,  C04B28/02 ,  C04B22/06 ,  C04B18/02 ,  C04B14/30 ,  C04B14/02 ,  C04B18/16 ,  C09K5/14

Abstract: A geopolymer thermal energy storage (TES) concrete product comprising at least one binder; at least one alkali activator; at least one fine aggregate with high thermal conductivity and heat capacity; and at least one coarse aggregate with high thermal conductivity and heat capacity.

公开(公告)号：US20190330643A1

公开(公告)日：2019-10-31

申请号：US16355932

申请日：2019-03-18

Applicant: The Catholic University of America

Inventor： Venigalla B. Rao ,  Pan Tao

IPC: C12N15/70 ,  C12N15/11 ,  C12N9/22

Abstract: Embodiments of the invention provide systems, methods, and kits for CRISPR-based editing of DNA targets by a CRISPR-associated (Cas) enzyme. The systems include a bacterial host cell adapted to produce an engineered bacteriophage comprising a Cas protein and guide RNA that do not naturally occur together, i.e. they are engineered to occur together, as well as a DNA repair template comprising a donor DNA having a desired mutation. The guide RNA comprises a trans-activating crRNA and a guide sequence complementary to a target protospacer in a bacteriophage genome. A wild-type bacteriophage or a glucosylhydroxymethyl cytosine (ghmC)-unmodified mutant bacteriophage may be delivered into a disclosed bacterial host cell to create recombinants of bacteriophage having the desired mutation provided by the donor DNA.

公开(公告)号：US10407470B2

公开(公告)日：2019-09-10

申请号：US15908372

申请日：2018-02-28

Applicant: The Catholic University of America

Inventor： Venigalla B. Rao ,  Wadad Alsalmi

IPC: C07K14/16 ,  C07K14/005 ,  C12N7/00 ,  A61K9/70 ,  A61K39/21 ,  C07K1/22 ,  C07K1/36 ,  A61K39/12 ,  A61K39/00

Abstract: Provided herein are HIV vaccines that encompasses recombinant trimers that mimic native HIV-1 envelope trimers. Also provided are methods of administering to a subject in need thereof an HIV vaccine provided herein to elicit antibodies against a recombinant trimer in the subject. A recombinant trimer is formed by a recombinant protein comprising a recombinant HIV-1 gp140 fused to a tag through a linker at C-terminus of the recombinant HIV-1 gp140, wherein the linker is sufficiently long so that the tag is accessible for binding by a binding molecule bound on a solid matrix during purification of the recombinant trimer.

公开(公告)号：US09850288B2

公开(公告)日：2017-12-26

申请号：US14806735

申请日：2015-07-23

Applicant: THE CATHOLIC UNIVERSITY OF AMERICA

Inventor： Venigalla B. Rao ,  Wadad Alsalmi

IPC: A61K39/21 ,  C07K14/005 ,  C12N7/00 ,  A61K9/70 ,  C07K1/22 ,  C07K1/36 ,  A61K39/12 ,  C07K14/16 ,  A61K39/00

CPC classification number: C07K14/005 ,  A61K9/7023 ,  A61K39/00 ,  A61K39/12 ,  A61K39/21 ,  A61K2039/54 ,  A61K2039/627 ,  C07K1/22 ,  C07K1/36 ,  C07K14/162 ,  C07K2319/00 ,  C07K2319/20 ,  C07K2319/21 ,  C07K2319/22 ,  C07K2319/40 ,  C07K2319/50 ,  C12N7/00 ,  C12N2740/16022 ,  C12N2740/16043 ,  C12N2740/16051 ,  C12N2740/16111 ,  C12N2740/16122 ,  C12N2740/16134 ,  C12N2740/16234

Abstract: An approach of producing recombinant trimers that mimic native HIV-1 envelope trimers is developed. A recombinant protein forming the recombinant trimers encompasses a recombinant HIV-1 gp140 fused to a tag through a linker at C-terminus of the recombinant HIV-1 gp140. The linker is sufficiently long so that the tag is accessible for binding by a binding molecule bound on a solid matrix. After expressed in a cell, the recombinant protein is secreted into the culture medium and assembles into recombinant trimers therein. The recombinant trimers may be directly purified from the culture medium. Cleaved and uncleaved trimers from different clade viruses are produced.

公开(公告)号：US09523101B2

公开(公告)日：2016-12-20

申请号：US14322097

申请日：2014-07-02

Applicant: The Catholic University of America

Inventor： Venigalla B. Rao

IPC: C12N15/86 ,  C12N15/87 ,  C07K14/005 ,  C12N7/00 ,  C12N15/88 ,  A61K47/48 ,  A61K31/7088 ,  A61K31/711 ,  A61K48/00

CPC classification number: C12N15/86 ,  A61K31/7088 ,  A61K31/711 ,  A61K47/48776 ,  A61K47/6901 ,  A61K48/00 ,  C07K14/005 ,  C12N7/00 ,  C12N15/87 ,  C12N15/88 ,  C12N2795/10042 ,  C12N2795/10122 ,  C12N2795/10142 ,  C12N2795/10152

Abstract: Complex viruses are assembled from simple protein subunits by sequential and irreversible assembly. During genome packaging in bacteriophages, a powerful molecular motor assembles at the special portal vertex of an empty prohead to initiate packaging. An aspect of the invention relates to the phage T4 packaging machine being highly promiscuous, translocating DNA into finished phage heads as well as into proheads. Single motors can force exogenous DNA into phage heads at the same rate as into proheads and phage heads undergo repeated initiations, packaging multiple DNA molecules into the same head. This shows that the phage DNA packaging machine has unusual conformational plasticity, powering DNA into an apparently passive capsid receptacle, including the highly stable virus shell, until it is full. These features allow for the design of a novel class of nanocapsid delivery vehicles.