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公开(公告)号:US09222944B2
公开(公告)日:2015-12-29
申请号:US13190885
申请日:2011-07-26
申请人: Yutaka Ishiwatari , Yuko Kai , Takami Maekawa , Yuzuru Eto
发明人: Yutaka Ishiwatari , Yuko Kai , Takami Maekawa , Yuzuru Eto
CPC分类号: G01N33/6872 , G01N33/5008
摘要: By contacting a test substance with a cell that expresses a Kv3.2 protein, and comparing observed cation influx into the cell with cation influx into the cell observed when the test substance is not contacted with the cell, a salty taste modulating substance such as salt alternative compound, or salt perception enhancing or inhibiting compound is screened.
摘要翻译: 通过使测试物质与表达Kv3.2蛋白质的细胞接触,并且当测试物质不与细胞接触时观察到的阳离子进入细胞,将观察到的阳离子流入细胞与阳离子进入比较,将咸味调节物质如盐 替代化合物或盐感知增强或抑制化合物被筛选。
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公开(公告)号:US20070190602A1
公开(公告)日:2007-08-16
申请号:US11316926
申请日:2005-12-27
申请人: Isao Abe , Rie Takeshita , Seiichi Hara , Sonoko Suzuki , Kenzo Yokozeki , Masakazu Sugiyama , Shunichi Suzuki , Kunihiko Watanabe , Nobuhisa Shimba , Takefumi Nakamura , Uno Tagami , Yuya Kodama , Hiromi Onoye , Reiko Yuuji , Eiichiro Suzuki , Tatsuki Kashiwagi , Ningchun Xu , Yuko Kai
发明人: Isao Abe , Rie Takeshita , Seiichi Hara , Sonoko Suzuki , Kenzo Yokozeki , Masakazu Sugiyama , Shunichi Suzuki , Kunihiko Watanabe , Nobuhisa Shimba , Takefumi Nakamura , Uno Tagami , Yuya Kodama , Hiromi Onoye , Reiko Yuuji , Eiichiro Suzuki , Tatsuki Kashiwagi , Ningchun Xu , Yuko Kai
CPC分类号: C12N9/93
摘要: The present invention aims at providing an excellent peptide-synthesizing protein and a method for efficiently producing a peptide. The peptide is synthesized by reacting an amine component and a carboxy component in the presence of at least one of proteins shown in the following (I) and (II).(I) The mutant protein having an amino acid sequence comprising one or more mutations from any of the mutations 1 to 68, and the mutations 239 to 290 and 324 to 377 in an amino acid sequence of SEQ ID NO:2.(II) The mutant protein having an amino acid sequence comprising one or more mutations from any of the mutations L1 to L335 and M1 to M642 in an amino acid sequence of SEQ ID NO:208
摘要翻译: 本发明的目的在于提供优异的肽合成蛋白质和有效生产肽的方法。 在下列(I)和(II)所示的至少一种蛋白质存在下,使胺组分和羧基组分反应合成肽。 (I)具有包含SEQ ID NO:2的氨基酸序列中的任何突变1至68和突变239至290和324至377的一个或多个突变的氨基酸序列的突变蛋白。 (II)具有包含SEQ ID NO:208的氨基酸序列中的任何突变L1至L335和M1至M642的一个或多个突变的氨基酸序列的突变蛋白
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公开(公告)号:US20050112731A1
公开(公告)日:2005-05-26
申请号:US10892112
申请日:2004-07-16
申请人: Tatsuki Kashiwagi , Yuko Kai , Kohki Ishikawa , Ei-ichiro Suzuki , Hiroshi Takagi , Mikhail Ziyatdinov , Ekaterina Redkina , Mikhail Gusyatiner
发明人: Tatsuki Kashiwagi , Yuko Kai , Kohki Ishikawa , Ei-ichiro Suzuki , Hiroshi Takagi , Mikhail Ziyatdinov , Ekaterina Redkina , Mikhail Gusyatiner
CPC分类号: C12P13/12 , C12N9/1029
摘要: O-acetylserine, L-cysteine and sulphurous compounds derived therefrom may be produced using a bacterium belonging to the genus Escherichia which harbors a mutant feedback-resistant serine acetyltransferases in which the amino acid sequence corresponding to positions from 89 to 96 in a wild-type serine acetyltransferase is replaced with any one of the amino acid sequences shown in SEQ ID NOS: 4 to 9, and feedback inhibition by L-cysteine in the bacterium is desensitized.
摘要翻译: O-乙酰丝氨酸,L-半胱氨酸和从其衍生的亚硫酸化合物可以使用属于埃希氏杆菌属的细菌产生,其含有突变反馈抗性丝氨酸乙酰转移酶,其中对应于野生型的89至96位的氨基酸序列 丝氨酸乙酰转移酶被SEQ ID NO:4至9所示的氨基酸序列中的任何一个替代,并且细菌中L-半胱氨酸的反馈抑制被脱敏。
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公开(公告)号:US07312058B2
公开(公告)日:2007-12-25
申请号:US10892112
申请日:2004-07-16
申请人: Tatsuki Kashiwagi , Yuko Kai , Kohki Ishikawa , Ei-ichiro Suzuki , Hiroshi Takagi , Mikhail Kharisovich Ziyatdinov , Ekaterina Igorevna Redkina , Mikhail Markovich Gusyatiner
发明人: Tatsuki Kashiwagi , Yuko Kai , Kohki Ishikawa , Ei-ichiro Suzuki , Hiroshi Takagi , Mikhail Kharisovich Ziyatdinov , Ekaterina Igorevna Redkina , Mikhail Markovich Gusyatiner
CPC分类号: C12P13/12 , C12N9/1029
摘要: O-acetylserine, L-cysteine and sulphurous compounds derived therefrom may be produced using a bacterium belonging to the genus Escherichia which harbors a mutant feedback-resistant serine acetyltransferases in which the amino acid sequence corresponding to positions from 89 to 96 in a wild-type serine acetyltransferase is replaced with any one of the amino acid sequences shown in SEQ ID NOS: 4 to 9, and feedback inhibition by L-cysteine in the bacterium is desensitized.
摘要翻译: O-乙酰丝氨酸,L-半胱氨酸和从其衍生的亚硫酸化合物可以使用属于埃希氏杆菌属的细菌产生,其含有突变反馈抗性丝氨酸乙酰转移酶,其中对应于野生型的89至96位的氨基酸序列 丝氨酸乙酰转移酶被SEQ ID NO:4至9所示的氨基酸序列中的任何一个替代,并且细菌中L-半胱氨酸的反馈抑制被脱敏。
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公开(公告)号:US20120028263A1
公开(公告)日:2012-02-02
申请号:US13190885
申请日:2011-07-26
申请人: Yutaka Ishiwatari , Yuko Kai , Takami Maekawa , Yuzuru Eto
发明人: Yutaka Ishiwatari , Yuko Kai , Takami Maekawa , Yuzuru Eto
IPC分类号: C12Q1/68 , C07H21/04 , C07K14/435 , C12Q1/02 , C12N5/00
CPC分类号: G01N33/6872 , G01N33/5008
摘要: By contacting a test substance with a cell that expresses a Kv3.2 protein, and comparing observed cation influx into the cell with cation influx into the cell observed when the test substance is not contacted with the cell, a salty taste modulating substance such as salt alternative compound, or salt perception enhancing or inhibiting compound is screened.
摘要翻译: 通过使测试物质与表达Kv3.2蛋白质的细胞接触,并且当测试物质不与细胞接触时观察到阳离子进入细胞的阳离子流入细胞中观察到的阳离子流入,将盐调味物质如盐 替代化合物或盐感知增强或抑制化合物被筛选。
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公开(公告)号:US20070292916A1
公开(公告)日:2007-12-20
申请号:US11765926
申请日:2007-06-20
申请人: Isao ABE , Rie Takeshita , Seiichi Hara , Sonoko Suzuki , Kenzo Yokozeki , Masakazu Sugiyama , Shunichi Suzuki , Kunihiko Watanabe , Nobuhisa Shimba , Takefumi Nakamura , Uno Tagami , Yuya Kodama , Hiromi Onoye , Reiko Yuuji , Eiichiro Suzuki , Tatsuki Kashiwagi , Ningchun Xu , Yuko Kai
发明人: Isao ABE , Rie Takeshita , Seiichi Hara , Sonoko Suzuki , Kenzo Yokozeki , Masakazu Sugiyama , Shunichi Suzuki , Kunihiko Watanabe , Nobuhisa Shimba , Takefumi Nakamura , Uno Tagami , Yuya Kodama , Hiromi Onoye , Reiko Yuuji , Eiichiro Suzuki , Tatsuki Kashiwagi , Ningchun Xu , Yuko Kai
CPC分类号: C07K14/00 , C07K14/195 , C12N9/93
摘要: The present invention aims at providing an excellent peptide-synthesizing protein and a method for efficiently producing a peptide. The peptide is synthesized by reacting an amine component and a carboxy component in the presence of at least one of proteins shown in the following (I) and (II). (I) The mutant protein having an amino acid sequence comprising one or more mutations from any of the mutations 1 to 68, and the mutations 239 to 290 and 324 to 377 in an amino acid sequence of SEQ ID NO:2. (II) The mutant protein having an amino acid sequence comprising one or more mutations from any of the mutations L1 to L335 and M1 to M642 in an amino acid sequence of SEQ ID NO:208
摘要翻译: 本发明的目的在于提供优异的肽合成蛋白质和有效生产肽的方法。 在下列(I)和(II)所示的至少一种蛋白质存在下,使胺组分和羧基组分反应合成肽。 (I)具有包含SEQ ID NO:2的氨基酸序列中的任何突变1至68和突变239至290和324至377的一个或多个突变的氨基酸序列的突变蛋白。 (II)具有包含SEQ ID NO:208的氨基酸序列中的任何突变L1至L335和M1至M642的一个或多个突变的氨基酸序列的突变蛋白
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