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公开(公告)号：US20050112731A1
公开(公告)日：2005-05-26
申请号：US10892112
申请日：2004-07-16
申请人： Tatsuki Kashiwagi , Yuko Kai , Kohki Ishikawa , Ei-ichiro Suzuki , Hiroshi Takagi , Mikhail Ziyatdinov , Ekaterina Redkina , Mikhail Gusyatiner
发明人： Tatsuki Kashiwagi , Yuko Kai , Kohki Ishikawa , Ei-ichiro Suzuki , Hiroshi Takagi , Mikhail Ziyatdinov , Ekaterina Redkina , Mikhail Gusyatiner
CPC分类号： C12P13/12 , C12N9/1029
摘要： O-acetylserine, L-cysteine and sulphurous compounds derived therefrom may be produced using a bacterium belonging to the genus Escherichia which harbors a mutant feedback-resistant serine acetyltransferases in which the amino acid sequence corresponding to positions from 89 to 96 in a wild-type serine acetyltransferase is replaced with any one of the amino acid sequences shown in SEQ ID NOS: 4 to 9, and feedback inhibition by L-cysteine in the bacterium is desensitized.
摘要翻译： O-乙酰丝氨酸，L-半胱氨酸和从其衍生的亚硫酸化合物可以使用属于埃希氏杆菌属的细菌产生，其含有突变反馈抗性丝氨酸乙酰转移酶，其中对应于野生型的89至96位的氨基酸序列 丝氨酸乙酰转移酶被SEQ ID NO：4至9所示的氨基酸序列中的任何一个替代，并且细菌中L-半胱氨酸的反馈抑制被脱敏。
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公开(公告)号：US07312058B2
公开(公告)日：2007-12-25
申请号：US10892112
申请日：2004-07-16
申请人： Tatsuki Kashiwagi , Yuko Kai , Kohki Ishikawa , Ei-ichiro Suzuki , Hiroshi Takagi , Mikhail Kharisovich Ziyatdinov , Ekaterina Igorevna Redkina , Mikhail Markovich Gusyatiner
发明人： Tatsuki Kashiwagi , Yuko Kai , Kohki Ishikawa , Ei-ichiro Suzuki , Hiroshi Takagi , Mikhail Kharisovich Ziyatdinov , Ekaterina Igorevna Redkina , Mikhail Markovich Gusyatiner
CPC分类号： C12P13/12 , C12N9/1029
摘要： O-acetylserine, L-cysteine and sulphurous compounds derived therefrom may be produced using a bacterium belonging to the genus Escherichia which harbors a mutant feedback-resistant serine acetyltransferases in which the amino acid sequence corresponding to positions from 89 to 96 in a wild-type serine acetyltransferase is replaced with any one of the amino acid sequences shown in SEQ ID NOS: 4 to 9, and feedback inhibition by L-cysteine in the bacterium is desensitized.
摘要翻译： O-乙酰丝氨酸，L-半胱氨酸和从其衍生的亚硫酸化合物可以使用属于埃希氏杆菌属的细菌产生，其含有突变反馈抗性丝氨酸乙酰转移酶，其中对应于野生型的89至96位的氨基酸序列 丝氨酸乙酰转移酶被SEQ ID NO：4至9所示的氨基酸序列中的任何一个替代，并且细菌中L-半胱氨酸的反馈抑制被脱敏。
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公开(公告)号：US07553650B2
公开(公告)日：2009-06-30
申请号：US11505438
申请日：2006-08-17
申请人： Tatsuki Kashiwagi , Nobuhisa Shimba , Kohki Ishikawa , Ei-ichiro Suzuki , Keiichi Yokoyama , Kazuo Hirayama
发明人： Tatsuki Kashiwagi , Nobuhisa Shimba , Kohki Ishikawa , Ei-ichiro Suzuki , Keiichi Yokoyama , Kazuo Hirayama
CPC分类号： C12N9/1044 , C07K14/195 , C07K2299/00 , C12N15/102 , C12Y203/02013
摘要： The present invention relates to a method for designing and preparing mutant transglutaminases on the basis of the three-dimensional structure of MTG derived from Streptoverticillium mobaraense (MTG), and the mutant MTG thus prepared. The present invention provides a method for modifying MTG on the basis of the three-dimensional structure, and transglutaminase having reactivity on the substrate improved by the method. In the present invention, the binding site of MTG for the substrate is extrapolated based on the three-dimensional structure obtained by X-ray crystal structure analysis of MTG crystals, and the mutant transglutaminases are designed and produced by replacing, inserting or deleting amino acid residues positioned at the substrate-binding site of the transglutaminase.
摘要翻译： 本发明涉及基于来自链霉菌（MTG）的MTG的三维结构和由此制备的突变体MTG来设计和制备突变转谷氨酰胺酶的方法。 本发明提供了一种基于三维结构修饰MTG的方法，并且通过该方法改善了在基板上具有反应性的转谷氨酰胺酶。 在本发明中，基于通过MTG晶体的X射线晶体结构分析获得的三维结构外推基质的MTG的结合位点，通过置换，插入或缺失氨基酸来设计和生产突变型转谷氨酰胺酶 位于转谷氨酰胺酶底物结合位点的残基。
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公开(公告)号：US20060275872A1
公开(公告)日：2006-12-07
申请号：US11505438
申请日：2006-08-17
申请人： Tatsuki Kashiwagi , Nobuhisa Shimba , Kohki Ishikawa , Ei-ichiro Suzuki , Keiichi Yokoyama , Kazuo Hirayama
发明人： Tatsuki Kashiwagi , Nobuhisa Shimba , Kohki Ishikawa , Ei-ichiro Suzuki , Keiichi Yokoyama , Kazuo Hirayama
CPC分类号： C12N9/1044 , C07K14/195 , C07K2299/00 , C12N15/102 , C12Y203/02013
摘要： The present invention relates to a method for designing and preparing mutant transglutaminases on the basis of the three-dimensional structure of MTG derived from Streptoverticillium mobaraense (MTG), and the mutant MTG thus prepared. The present invention provides a method for modifying MTG on the basis of the three-dimensional structure, and transglutaminase having reactivity on the substrate improved by the method. In the present invention, the binding site of MTG for the substrate is extrapolated based on the three-dimensional structure obtained by X-ray crystal structure analysis of MTG crystals, and the mutant transglutaminases are designed and produced by replacing, inserting or deleting amino acid residues positioned at the substrate-binding site of the transglutaminase.
摘要翻译： 本发明涉及基于来自链霉菌（MTG）的MTG的三维结构和由此制备的突变体MTG来设计和制备突变转谷氨酰胺酶的方法。 本发明提供了一种基于三维结构修饰MTG的方法，并且通过该方法改善了在基板上具有反应性的转谷氨酰胺酶。 在本发明中，基于通过MTG晶体的X射线晶体结构分析获得的三维结构外推基质的MTG的结合位点，通过置换，插入或缺失氨基酸来设计和生产突变型转谷氨酰胺酶 位于转谷氨酰胺酶底物结合位点的残基。
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公开(公告)号：US06987008B1
公开(公告)日：2006-01-17
申请号：US09807990
申请日：2000-09-01
申请人： Kohki Ishikawa , Ei-ichiro Suzuki , Keiko Gondoh , Nobuhisa Shimba , Yasuhiro Mihara , Hisashi Kawasaki , Osamu Kurahashi , Tohru Kouda , Megumi Shimaoka , Rie Kozutsumi , Yasuhisa Asano
发明人： Kohki Ishikawa , Ei-ichiro Suzuki , Keiko Gondoh , Nobuhisa Shimba , Yasuhiro Mihara , Hisashi Kawasaki , Osamu Kurahashi , Tohru Kouda , Megumi Shimaoka , Rie Kozutsumi , Yasuhisa Asano
摘要： There is provided a mutant nucleoside-5′-phosphate producing enzyme with improved nucleoside-5′-phosphate producing ability of a nucleoside-5′-phosphate producing enzyme that has transphosphorylation activity and/or phosphatase activity and has one Lys residue, two Arg residues and two His residues with distances between their Cα's within a particular ranges and a space around them allowing a binding of a nucleoside, of which mutation is designed by using the crystal structure of EB-AP.
摘要翻译： 提供了具有转移磷酸化活性和/或磷酸酶活性并具有一个Lys残基的核苷-5'-磷酸生产酶的核苷-5'-磷酸生产能力的突变体核苷-5'-磷酸生产酶，两个Arg 残留物和两个His残基，其特定范围内的Calpha与其周围的空间之间的距离允许结合核苷，其中通过使用EB-AP的晶体结构设计突变。
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公开(公告)号：US20130316572A1
公开(公告)日：2013-11-28
申请号：US13956954
申请日：2013-08-01
申请人： Kohki Ishikawa , Shigeru Ishikawa
发明人： Kohki Ishikawa , Shigeru Ishikawa
IPC分类号： H01R13/621
CPC分类号： H01R13/621 , H01R13/641 , H01R2201/26
摘要： A connector assembly (1) comprises: a female connector (60) which includes a female terminal (70), a female-side housing (80) which holds the female terminal (70), and a bolt (86) which is held to be rotatable by the female-side housing (80); and a male connector (10) which includes a male terminal (20), a male-side housings (30 and 50) which holds the male terminal (20) and can be fitted with the female-side housing (80), and a female screw portion (531) which is provided in the male-side outer housing (50), the female connector (60) and the male connector (10) fit together by screwing the bolt (86) and the female screw portion (531) with each other, and the connector assembly (1) comprise a fitting indicator (514 and 824) which indicates fitting between the female terminal (70) and the male terminal (20).
摘要翻译： 连接器组件（1）包括：阴连接器（60），其包括阴端子（70），保持阴端子（70）的阴侧壳体（80）和螺栓（86） 可通过阴侧壳体（80）旋转; 以及阳连接器（10），其包括阳端子（20），保持阳端子（20）并可与阴侧壳体（80）配合的阳侧壳体（30和50），以及 设置在阳侧外壳（50）中的阴螺纹部（531），阴连接器（60）和阳连接器（10）通过螺栓（86）和阴螺纹部（531）的螺合而嵌合在一起， 并且连接器组件（1）包括装配指示器（514和824），其指示阴端子（70）和阳端子（20）之间的配合。
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公开(公告)号：US08276963B2
公开(公告)日：2012-10-02
申请号：US11313618
申请日：2005-12-22
IPC分类号： B60R7/04
摘要： A storage structure, including: a main body portion including an opening portion and a cable outlet portion; a lid slidably mounted on the main body portion to open and close the opening portion, the lid including a cable introductory portion; and a flat plate-shaped cable including a first end at the cable outlet portion, a second end at the cable introductory portion, and a cable slack portion between the first and second ends, where the length of the cable slack portion between the cable outlet portion and the cable introductory portion is set to be equal to or more than the distance between the cable outlet portion and the cable introductory portion, both when the lid is positioned to open and to close the opening portion.
摘要翻译： 一种存储结构，包括：主体部分，包括开口部分和电缆出口部分; 可滑动地安装在主体部分上以打开和关闭开口部分的盖子，盖子包括一个电缆引导部分; 以及平板状电缆，包括在电缆出口部分处的第一端，电缆引导部分处的第二端，以及在第一和第二端之间的电缆松弛部分，其中电缆出口部分之间的电缆松弛部分的长度 当盖被定位成打开并关闭开口部分时，电缆引导部分被设定为等于或大于电缆出口部分和电缆引导部分之间的距离。
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公开(公告)号：US20110051393A1
公开(公告)日：2011-03-03
申请号：US12988189
申请日：2009-04-16
申请人： Kohki Ishikawa , Kiyotsugu Oba , Mitsuru Kamikatano , Kiyoshi Ina
发明人： Kohki Ishikawa , Kiyotsugu Oba , Mitsuru Kamikatano , Kiyoshi Ina
IPC分类号： G01D11/28
CPC分类号： G01D11/28 , B60K2370/20 , B60K2370/33
摘要： Both end portions of a light guide body (3) are bent in respective directions towards a second main surface to form guide portions (31, 32). Light that is emitted from each light source (4, 5) mounted on a wiring board arranged on the rear side of the light guide body and that travels in a direction vertical to main surfaces of the light guide body is guided by each guide portion (31, 32) into light propagating in a direction along the main surfaces of the light guide body. Reflection surfaces of a plurality of grooves (13) formed on the light guide body radially from the center position thereof reflect the light from the light sources to a dial plate (2) side and a diffusion printing diffuses the reflected light, so that the diffused light transmissively illuminates a scale marks section (21) and a numerical characters section (22) of the dial plate with uniform brightness.
摘要翻译： 导光体（3）的两端部朝向第二主表面朝各个方向弯曲以形成引导部（31,32）。 安装在布置在导光体背面侧并沿与导光体主表面垂直的方向移动的布线基板上的每个光源（4,5）发出的光由各引导部分 31,32）沿着沿着导光体的主表面的方向传播的光。 从导光体的中心位置径向形成的多个凹槽（13）的反射面将来自光源的光反射到表盘（2）侧，扩散印刷使反射光扩散， 光透明地以均匀的亮度照亮刻度盘部分（21）和数字字符部分（22）。
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公开(公告)号：US09484647B2
公开(公告)日：2016-11-01
申请号：US13956903
申请日：2013-08-01
申请人： Kohki Ishikawa , Shigeru Ishikawa , Kazunori Sato
发明人： Kohki Ishikawa , Shigeru Ishikawa , Kazunori Sato
IPC分类号： H01R9/24 , H01R13/502 , H01R13/621 , H01R43/00 , H01R105/00 , H01R11/12 , H01R13/52
CPC分类号： H01R9/24 , H01R11/12 , H01R13/502 , H01R13/521 , H01R13/6215 , H01R43/005 , H01R2105/00 , H01R2201/26
摘要： A male connector (10) comprises: a male-side inner housing (30) which holds a plurality of male terminals (20) so as to expose circular portions (22) of the male terminals (20); and a partition member (40) which includes a rib (41) dividing the circular portions (22) of the plurality of the male terminals (30), and the male-side inner housing (30) and the partition member (40) are configured to be separate from each other.
摘要翻译： 公连接器（10）包括：阳侧内壳（30），其保持多个阳端子（20）以暴露阳端子（20）的圆形部分（22）; 以及分隔构件（40），其包括分割多个阳端子（30）的圆形部分（22）和阳侧内壳体（30）和分隔构件（40）的肋（41） 被配置为彼此分开。
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公开(公告)号：US20060166557A1
公开(公告)日：2006-07-27
申请号：US11313618
申请日：2005-12-22
IPC分类号： H01R9/03
摘要： A storage structure, including: a main body portion including an opening portion and a cable outlet portion; a lid slidably mounted on the main body portion to open and close the opening portion, the lid including a cable introductory portion; and a flat plate-shaped cable including a first end at the cable outlet portion, a second end at the cable introductory portion, and a cable slack portion between the first and second ends, where the length of the cable slack portion between the cable outlet portion and the cable introductory portion is set to be equal to or more than the distance between the cable outlet portion and the cable introductory portion, both when the lid is positioned to open and to close the opening portion.
摘要翻译： 一种存储结构，包括：主体部分，包括开口部分和电缆出口部分; 可滑动地安装在主体部分上以打开和关闭开口部分的盖子，盖子包括电缆引导部分; 以及平板状电缆，包括在电缆出口部分处的第一端，电缆引导部分处的第二端，以及在第一和第二端之间的电缆松弛部分，其中电缆出口部分之间的电缆松弛部分的长度 当盖被定位成打开并关闭开口部分时，电缆引导部分被设定为等于或大于电缆出口部分和电缆引导部分之间的距离。
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