公开(公告)号：US10683551B2

公开(公告)日：2020-06-16

申请号：US15411855

申请日：2017-01-20

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Steven T. Okino ,  Yan Wang

IPC: C12Q1/68 ,  C12Q1/6886 ,  C12Q1/6806 ,  C12Q1/6809 ,  C12Q1/6804

Abstract: This invention provides methods of determining the biological, pathological, genetic, epigenetic or disease status in a biological sample by determining the methylation status of a subpopulation of genomic DNA in the sample.

公开(公告)号：US20180230462A1

公开(公告)日：2018-08-16

申请号：US15895504

申请日：2018-02-13

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Xiao-Song Gong ,  Yan Wang

IPC: C12N15/10 ,  C12Q1/686 ,  C12N9/22

CPC classification number: C12N15/1096 ,  C12N9/22 ,  C12N2770/00022 ,  C12Q1/6844 ,  C12Q1/686 ,  C12Q2521/107 ,  C12Q2521/327

Abstract: Hybrid reverse transcriptases formed from portions of FLVRT and MLVRT are provided.

公开(公告)号：US09994887B2

公开(公告)日：2018-06-12

申请号：US14482756

申请日：2014-09-10

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Xiao-Song Gong ,  John Sullivan ,  Rongdian Fu ,  Yan Wang ,  Evan H. Bursey ,  Man Cheng

IPC: C12Q1/68 ,  C12P19/34 ,  C07K16/40 ,  C12N9/12

CPC classification number: C12P19/34 ,  C07K16/40 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/76 ,  C07K2319/80 ,  C12N9/1252 ,  C12Q1/6804 ,  C12Q1/686 ,  C12Q2531/113 ,  C12Q2527/125 ,  C12Q2521/101

Abstract: The present invention provides methods and compositions for improving the efficiency of nucleic acid amplification reactions. The invention encompasses hybrid polymerases that show increased processivity over wild type polymerases as well as decreased exonucleases activity. The invention also encompasses methods, compositions and kits for conducting nucleic acid synthesis and amplification reactions in which non-specific amplification of primers is reduced.

公开(公告)号：US09708598B2

公开(公告)日：2017-07-18

申请号：US14831042

申请日：2015-08-20

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Yan Wang ,  Peter Vander Horn ,  Lei Xi

IPC: C12P19/34 ,  C12N15/10 ,  C07K14/195 ,  C12N9/12 ,  C12N15/62 ,  C12Q1/68

CPC classification number: C12N15/10 ,  C07K14/195 ,  C07K2319/00 ,  C07K2319/80 ,  C12N9/1252 ,  C12N15/62 ,  C12P19/34 ,  C12Q1/686 ,  C12Y207/07007

Abstract: This invention provides for methods of sequencing and performing polymerase reactions using an improved generation of nucleic acid polymerases. The improvement is the fusion of a sequence-non-specific nucleic-acid-binding domain to the enzyme in a manner that enhances the processivity of the polymerase.

公开(公告)号：US20160060607A1

公开(公告)日：2016-03-03

申请号：US14845234

申请日：2015-09-03

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Peter B. Vander Horn ,  Yan Wang

IPC: C12N9/12

CPC classification number: C12N9/1252 ,  C07K2319/00 ,  C12N9/1241 ,  C12Y207/07007

Abstract: The invention provides novel compositions with polymerase activity and methods of using the compositions.

公开(公告)号：US09145550B2

公开(公告)日：2015-09-29

申请号：US13753207

申请日：2013-01-29

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Peter B. Vander Horn ,  Yan Wang

IPC: C12N9/12

CPC classification number: C12N9/1252 ,  C07K2319/00 ,  C12N9/1241 ,  C12Y207/07007

Abstract: The invention provides novel compositions with polymerase activity and methods of using the compositions.

公开(公告)号：US20130203119A1

公开(公告)日：2013-08-08

申请号：US13850048

申请日：2013-03-25

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Yan Wang

IPC: C12N9/96

CPC classification number: C12N9/1276 ,  C07K14/00 ,  C07K14/001 ,  C07K19/00 ,  C07K2319/80 ,  C12N9/1252 ,  C12N9/96 ,  C12P19/34 ,  C12Y207/07007 ,  C12Y207/07049 ,  Y02P20/52

Abstract: This invention provides for an improved generation of novel nucleic acid modifying enzymes. The improvement is the fusion of a sequence-non-specific nucleic-acid-binding domain to the enzyme in a manner that enhances the ability of the enzyme to bind and catalytically modify the nucleic acid.

公开(公告)号：US20130143300A1

公开(公告)日：2013-06-06

申请号：US13753207

申请日：2013-01-29

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Peter B. Vander Horn ,  Yan Wang

IPC: C12N9/12

CPC classification number: C12N9/1252 ,  C07K2319/00 ,  C12N9/1241 ,  C12Y207/07007

Abstract: The invention provides novel compositions with polymerase activity and methods of using the compositions.

公开(公告)号：US11649453B2

公开(公告)日：2023-05-16

申请号：US17211595

申请日：2021-03-24

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Xiao-Song Gong ,  Yan Wang

IPC: C12N9/10 ,  C12N9/22 ,  C12N15/10 ,  C12Q1/686 ,  C12Q1/6844

CPC classification number: C12N15/1096 ,  C12N9/22 ,  C12Q1/686 ,  C12N2770/00022 ,  C12Q1/6844 ,  C12Q1/6844 ,  C12Q2521/107 ,  C12Q2521/327

Abstract: Hybrid reverse transcriptases formed from portions of FLVRT and MLVRT are provided.

公开(公告)号：US11021735B2

公开(公告)日：2021-06-01

申请号：US16009894

申请日：2018-06-15

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Man Cheng ,  Xiao-Song Gong ,  Yan Wang ,  Adam M. McCoy

IPC: C12Q1/6806 ,  C12Q1/686 ,  C12P19/34 ,  C12Q1/6853 ,  C12N15/10

Abstract: Methods of detecting and amplifying short RNAs are provided.