公开(公告)号：US10981947B2

公开(公告)日：2021-04-20

申请号：US15122609

申请日：2015-03-12

Applicant: Cytiva Sweden AB

Inventor： Bjorn Markus Olovsson ,  Mathias Osmark ,  Mangus Gamberg ,  Daniel Carlsson

IPC: C07K1/04 ,  B65B31/04 ,  B01J19/00 ,  C07K1/06 ,  C07K1/08

Abstract: The invention relates to a container holder 10, comprising a main body 12 which in turn comprises a gas inlet 16; a solution liquid outlet 18; a gas control valve 20 through which a gas enters the container 100 from the gas inlet; and a sealing means 22 for the container, which sealing means includes a passageway 24 for the input of gas and output of a solution in the container via an egress tube 19; wherein when the container is connected to the container holder through the sealing means, the gas control valve opens automatically, and when the container is disconnected the gas control valve is closed automatically. The invention further relates to a container panel 50 which includes two or more container holders. Also disclosed are methods of using these containers and container panels for synthesizing a polypeptide.

公开(公告)号：US20210086101A1

公开(公告)日：2021-03-25

申请号：US17113311

申请日：2020-12-07

Applicant: Cytiva Sweden AB

Inventor： Klaus Gebauer ,  Bjorn Markus Olovsson

IPC: B01D15/18 ,  G01N30/20 ,  G01N30/88 ,  G01N30/46

Abstract: Disclosed is a device for chromatographic separations comprising: a manifold comprising a manifold body defining an elongate central duct, the central duct comprising a centrally-located closable duct valve providing selective fluid communication between a first portion of the central duct and an opposed second portion of the central duct, a first plurality of connectors, each connector of the first plurality of connectors for connecting to a distinct chromatographic separation column and/or feed or extraction tubing or to a connector of an adjacent manifold; a second plurality of connectors, each connector of the second plurality of connectors for connecting to a distinct chromatographic separation column and/or feed or extraction tubing or to a connector of an adjacent manifold; wherein said manifold body further defines: a first plurality of branch ducts, each branch duct of which extending from the first portion of the central duct to an individual one of the first plurality of connectors, each of the branch ducts of the first plurality of branch ducts comprising a closable branch valve providing selectable fluid communication between a respective connector and the first portion of the central duct, a second plurality of branch ducts, each branch duct of which extending from the second portion of the central duct to an individual one of the second plurality of connectors, each of the branch ducts of the second plurality of branch ducts comprising a closable branch valve providing selectable fluid communication between a respective connector and the second portion of the central duct; first and second ports in fluid communication with the centrally-located closable duct valve wherein said first port communicates with said first portion of the central duct and said second port communicates with said second portion of said central duct, wherein one of said first and second ports is further positioned to communicate with said central duct at a location between the centrally-located closable duct valve and the first and second plurality of branch ducts, respectively.

公开(公告)号：US10888799B2

公开(公告)日：2021-01-12

申请号：US15534540

申请日：2015-12-23

Applicant: Cytiva Sweden AB

Inventor： Klaus Gebauer ,  Bjorn Markus Olovsson

IPC: B01D15/18 ,  G01N30/20 ,  G01N30/42 ,  G01N30/46 ,  G01N30/88

Abstract: Disclosed is a device for chromatographic separations comprising: a manifold comprising a manifold body defining an elongate central duct, the central duct comprising a centrally-located closable duct valve providing selective fluid communication between a first portion of the central duct and an opposed second portion of the central duct, a first plurality of connectors, each connector of the first plurality of connectors for connecting to a distinct chromatographic separation column and/or feed or extraction tubing or to a connector of an adjacent manifold; a second plurality of connectors, each connector of the second plurality of connectors for connecting to a distinct chromatographic separation column and/or feed or extraction tubing or to a connector of an adjacent manifold; wherein said manifold body further defines: a first plurality of branch ducts, each branch duct of which extending from the first portion of the central duct to an individual one of the first plurality of connectors, each of the branch ducts of the first plurality of branch ducts comprising a closable branch valve providing selectable fluid communication between a respective connector and the first portion of the central duct, a second plurality of branch ducts, each branch duct of which extending from the second portion of the central duct to an individual one of the second plurality of connectors, each of the branch ducts of the second plurality of branch ducts comprising a closable branch valve providing selectable fluid communication between a respective connector and the second portion of the central duct; first and second ports in fluid communication with the centrally-located closable duct valve wherein said first port communicates with said first portion of the central duct and said second port communicates with said second portion of said central duct, wherein one of said first and second ports is further positioned to communicate with said central duct at a location between the centrally-located closable duct valve and the first and second plurality of branch ducts, respectively.

公开(公告)号：US20240150697A1

公开(公告)日：2024-05-09

申请号：US18550075

申请日：2022-04-25

Applicant: CYTIVA SWEDEN AB

Inventor： Klaus Gebauer ,  Tim Francois ,  Andreas Lundin ,  Bjorn Markus Olovsson ,  Kerstin Erickson ,  Tomas Dalmo

IPC: C12M1/00 ,  C12M1/36 ,  C12M3/00

CPC classification number: C12M23/40 ,  C12M23/28 ,  C12M23/44 ,  C12M29/04 ,  C12M29/06 ,  C12M41/48

Abstract: The present invention relates to a reconfigurable bioprocessing system (80) operable to perform a predetermined set of bioprocessing operations therein. The reconfigurable bioprocessing system (80) comprises a base unit (60) comprising a plurality of valve actuators (102). At least one of the plurality of valve actuators (102) is operable to releasably engage with at least part of a pinch valve cassette (103, 203) to control fluid flow through the pinch valve cassette (103, 203). At least part of the pinch valve cassette (103, 203) is removably attachable to the base unit (60) and the pinch valve cassette (103, 203) is provided adjacent to said plurality of valve actuators (102). The reconfigurable bioprocessing system (80) also includes a control system operable to selectively actuate respective of said plurality of valve actuators (102) to provide a valve configuration within the pinch valve cassette (103, 203) to enable the base unit (60) and the pinch valve cassette (103, 203) together to perform one of the predetermined set of bioprocessing operations. A flow kit (107) for use in reconfigurable bioprocessing systems (80) and various methods (1000) for reconfiguring bioprocessing systems (80) (e.g. automatically) are also disclosed.

公开(公告)号：US11801456B2

公开(公告)日：2023-10-31

申请号：US17156749

申请日：2021-01-25

Applicant: Cytiva Sweden AB

Inventor： Bjorn Markus Olovsson

IPC: B01D15/16 ,  G01N30/32 ,  G01N30/34 ,  B01F23/40 ,  B01F25/31 ,  B01F35/21 ,  B01F35/22 ,  B01D15/22 ,  G01N30/86 ,  B01F35/221 ,  B01F35/71

CPC classification number: B01D15/166 ,  B01D15/22 ,  B01F23/49 ,  B01F25/31 ,  B01F35/2132 ,  B01F35/2133 ,  B01F35/2211 ,  B01F35/7176 ,  G01N30/32 ,  G01N30/34 ,  B01D15/16 ,  G01N30/8658 ,  G01N2030/324 ,  G01N2030/347

Abstract: A system for preparing solutions for chromatography application is disclosed. The system comprises a T-joint for preparing a buffer solution by mixing at least one first solution and a second solution. The T-joint receives the second solution from a solution supply unit connected to the T-joint. Further one or more low pressure pumps supply the one or more first solutions into the T-joint. The high pressure pump collects the buffer solution and delivers it to a chromatography apparatus.

公开(公告)号：US10926191B2

公开(公告)日：2021-02-23

申请号：US15533405

申请日：2015-12-10

Applicant: Cytiva Sweden AB

Inventor： Bjorn Markus Olovsson

IPC: B01D15/16 ,  B01D15/22 ,  B01F3/08 ,  G01N30/32 ,  G01N30/34 ,  B01F5/04 ,  B01F15/00 ,  B01F15/02 ,  G01N30/86

Abstract: A system for preparing solutions for chromatography application is disclosed. The system comprises a T-joint for preparing a buffer solution by mixing at least one first solution and a second solution. The T-joint receives the second solution from a solution supply unit connected to the T-joint. Further one or more low pressure pumps supply the one or more first solutions into the T-joint. The high pressure pump collects the buffer solution and delivers it to a chromatography apparatus.

公开(公告)号：US12290762B2

公开(公告)日：2025-05-06

申请号：US18474000

申请日：2023-09-25

Applicant: Cytiva Sweden AB

Inventor： Bjorn Markus Olovsson

IPC: B01D15/16 ,  B01D15/22 ,  B01F23/40 ,  B01F25/31 ,  B01F35/21 ,  B01F35/22 ,  B01F35/221 ,  B01F35/71 ,  F04B23/04 ,  G01N30/32 ,  G01N30/34 ,  G01N30/86

Abstract: A system for preparing solutions for chromatography application is disclosed. The system comprises a T-joint for preparing a buffer solution by mixing at least one first solution and a second solution. The T-joint receives the second solution from a solution supply unit connected to the T-joint. Further one or more low pressure pumps supply the one or more first solutions into the T-joint. The high pressure pump collects the buffer solution and delivers it to a chromatography apparatus.

公开(公告)号：US20240009594A1

公开(公告)日：2024-01-11

申请号：US18474000

申请日：2023-09-25

Applicant: Cytiva Sweden AB

Inventor： Bjorn Markus Olovsson

IPC: B01D15/16 ,  G01N30/32 ,  G01N30/34 ,  B01F23/40 ,  B01F25/31 ,  B01F35/21 ,  B01F35/221 ,  B01F35/71 ,  B01D15/22

CPC classification number: B01D15/166 ,  G01N30/32 ,  G01N30/34 ,  B01F23/49 ,  B01F25/31 ,  B01F35/2132 ,  B01F35/2133 ,  B01F35/2211 ,  B01F35/7176 ,  B01D15/22 ,  G01N2030/347 ,  B01D15/16 ,  G01N30/8658

Abstract: A system for preparing solutions for chromatography application is disclosed. The system comprises a T-joint for preparing a buffer solution by mixing at least one first solution and a second solution. The T-joint receives the second solution from a solution supply unit connected to the T-joint. Further one or more low pressure pumps supply the one or more first solutions into the T-joint. The high pressure pump collects the buffer solution and delivers it to a chromatography apparatus.

公开(公告)号：US11648490B2

公开(公告)日：2023-05-16

申请号：US17113311

申请日：2020-12-07

Applicant: Cytiva Sweden AB

Inventor： Klaus Gebauer ,  Bjorn Markus Olovsson

IPC: B01D15/18 ,  G01N30/20 ,  G01N30/42 ,  G01N30/46 ,  G01N30/88

CPC classification number: B01D15/1842 ,  G01N30/20 ,  G01N30/461 ,  G01N30/466 ,  G01N30/467 ,  G01N30/88 ,  G01N30/42 ,  G01N30/468 ,  G01N2030/205 ,  G01N2030/8881

Abstract: Disclosed is a device for chromatographic separations comprising: a manifold comprising a manifold body defining an elongate central duct, the central duct comprising a centrally-located closable duct valve providing selective fluid communication between a first portion of the central duct and an opposed second portion of the central duct, a first plurality of connectors, each connector of the first plurality of connectors for connecting to a distinct chromatographic separation column and/or feed or extraction tubing or to a connector of an adjacent manifold; a second plurality of connectors, each connector of the second plurality of connectors for connecting to a distinct chromatographic separation column and/or feed or extraction tubing or to a connector of an adjacent manifold; wherein said manifold body further defines: a first plurality of branch ducts, each branch duct of which extending from the first portion of the central duct to an individual one of the first plurality of connectors, each of the branch ducts of the first plurality of branch ducts comprising a closable branch valve providing selectable fluid communication between a respective connector and the first portion of the central duct, a second plurality of branch ducts, each branch duct of which extending from the second portion of the central duct to an individual one of the second plurality of connectors, each of the branch ducts of the second plurality of branch ducts comprising a closable branch valve providing selectable fluid communication between a respective connector and the second portion of the central duct; first and second ports in fluid communication with the centrally-located closable duct valve wherein said first port communicates with said first portion of the central duct and said second port communicates with said second portion of said central duct, wherein one of said first and second ports is further positioned to communicate with said central duct at a location between the centrally-located closable duct valve and the first and second plurality of branch ducts, respectively.

公开(公告)号：US11571635B2

公开(公告)日：2023-02-07

申请号：US15562586

申请日：2016-03-23

Applicant: Cytiva Sweden AB

Inventor： Bjorn Markus Olovsson ,  Anders Ljunglof ,  Karol Maciej Lacki

IPC: B01D15/00 ,  B01D15/38 ,  B01D15/18 ,  B01D15/14 ,  G01N30/46 ,  C07K1/22 ,  C12N7/00 ,  G01N30/20 ,  G01N30/88

Abstract: The present invention relates to a liquid chromatography system for the separation of bio-molecules in a fluid including at least two unit operations, wherein the first unit operation is a step of multi-column chromatography and the second unit operation is a step modifying said bio-molecules and/or the fluid, wherein the modification comprises feeding the fluid resulting from the last chromatography column of the first unit operation into a system comprising at least two containers, wherein each container has a volume and a moveable sidewall arranged to divide the volume into a first sub-volume and a second sub-volume, and each container comprises a first port connected to the first volume and a second port connected to the second sub-volume. The invention also relates to a virus inactivation device for a chromatography system according to the invention, which enables continuous or semi-continuous processing of biomolecules, as well as a method of using such a device.