公开(公告)号：US20030218130A1

公开(公告)日：2003-11-27

申请号：US10412679

申请日：2003-04-14

Applicant: Ciphergen Biosystems, Inc.

Inventor： Egisto Boschetti ,  Jian Ding ,  Pierre Girot ,  Luc Gurrier ,  Christopher A. Pohl ,  Michael T. Grimes

IPC: H01J049/00 ,  B01D059/44

CPC classification number: G01N33/6848 ,  B01J19/0046 ,  B01J2219/00315 ,  B01J2219/00454 ,  B01J2219/00497 ,  B01J2219/00527 ,  B01J2219/00533 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00641 ,  B01J2219/00644 ,  B01J2219/00657 ,  B01J2219/00659 ,  B01J2219/00677 ,  B01J2219/00689 ,  B01J2219/00707 ,  B01J2219/00711 ,  B01J2219/00716 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00731 ,  B01J2219/00734 ,  B82Y30/00 ,  C08B11/20 ,  C08B15/00 ,  C08B31/00 ,  C08B33/00 ,  C08B37/0021 ,  C08B37/0039 ,  C08L5/02 ,  C40B40/06 ,  C40B40/10 ,  C40B40/12 ,  C40B40/14 ,  C40B50/14 ,  C40B60/14 ,  G01N33/5302 ,  G01N33/559 ,  G01N33/6851 ,  H01J49/0418

Abstract: The present invention provides a substrate having a polymerized, polysaccharide-based hydrogel attached to the surface. The hydrogel can be derivatized with binding functionalities that bind analytes from a sample. The invention further provides methods of using the device and gels that are capable of selectively binding one or more analytes from a sample.

Abstract translation: 本发明提供了具有附着在表面上的聚合的多糖基水凝胶的基材。 水凝胶可以用结合来自样品的分析物的结合官能团衍生化。 本发明还提供使用能够选择性地结合一种或多种来自样品的分析物的装置和凝胶的方法。

公开(公告)号：US20030032043A1

公开(公告)日：2003-02-13

申请号：US10197115

申请日：2002-07-16

Applicant: Ciphergen Biosystems, Inc.

Inventor： Christopher A. Pohl ,  Steven C. Papanu

IPC: C12Q001/68 ,  G01N033/53 ,  G01N033/542 ,  C12M001/34

CPC classification number: C40B40/10 ,  B01J19/0046 ,  B01J2219/00274 ,  B01J2219/00497 ,  B01J2219/005 ,  B01J2219/00509 ,  B01J2219/00527 ,  B01J2219/00576 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00648 ,  B01J2219/00689 ,  B01J2219/00702 ,  B01J2219/00707 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/0074 ,  B01L3/5085 ,  B01L2200/12 ,  B01L2300/069 ,  B01L2300/0819 ,  B01L2300/0887 ,  B82Y30/00 ,  C07B2200/11 ,  C40B40/06 ,  G01N33/54393

Abstract: The present invention provides an adsorbent chip, which includes three components, a substrate, an intermediate layer of linker arms and an adsorbent film, which is attached to the linker arms. The adsorbent film is made up of a plurality of adsorbent particles, each of which includes a binding functionality. The invention also provides a method of making the chips of the invention in which the substrate-intermediate film cassette is formed and the adsorbent film is subsequently immobilized thereon. When the adsorbent film is from the same preparation across a particular batch of chips, the chips provide for the acquisition of data that are highly reproducible from one chip to the next throughout the particular batch of chips. Additionally, the invention provides methods for using the chips to perform assays.

公开(公告)号：US20030124371A1

公开(公告)日：2003-07-03

申请号：US10289185

申请日：2002-11-05

Applicant: Ciphergen Biosystems, Inc.

Inventor： Pil-Je Um ,  Christopher A. Pohl

IPC: B32B027/00

CPC classification number: G01N33/54386 ,  B01J19/0046 ,  B01J2219/00527 ,  B01J2219/00576 ,  B01J2219/00581 ,  B01J2219/00641 ,  B01J2219/00659 ,  B01L3/5085 ,  B01L2300/069 ,  B01L2300/0819 ,  G01N1/405 ,  G01N33/54373 ,  G01N33/545 ,  G01N35/00029 ,  G01N2035/00158 ,  G11C13/0016 ,  G11C13/0019 ,  Y10T428/31855 ,  Y10T428/31935 ,  Y10T436/24

Abstract: The invention provides a water-swellable hydrophobic hydrogel and analytical devices incorporating the hydrogel of the invention. Also provided are methods of using the hydrogel to prepare the analytical devices and methods of using the analytical devices to detect, quantitate and discriminate between analytes in a sample. Moreover, the invention provides kits that include components of a hydrogel and instructions for making a chip with a hydrophobic surface.

Abstract translation: 本发明提供了水溶胀性疏水性水凝胶和掺入本发明的水凝胶的分析装置。 还提供了使用水凝胶来制备分析装置的方法以及使用分析装置来检测，定量和区分样品中的分析物的方法。 此外，本发明提供了包括水凝胶的组分的试剂盒以及制备具有疏水性表面的芯片的说明书。

公开(公告)号：US20030017464A1

公开(公告)日：2003-01-23

申请号：US09908518

申请日：2001-07-17

Applicant: Ciphergen Biosystems, Inc.

Inventor： Christopher A. Pohl

IPC: C12Q001/68 ,  G01N033/53 ,  G01N033/542 ,  C12M001/34

CPC classification number: B82Y30/00 ,  B01J19/0046 ,  B01J2219/00274 ,  B01J2219/00576 ,  B01J2219/00648 ,  B01J2219/00702 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/0074 ,  B01L3/5085 ,  B01L2200/12 ,  B01L2300/069 ,  B01L2300/0819 ,  B01L2300/0887 ,  C07B2200/11 ,  C40B40/06 ,  C40B40/10 ,  G01N33/54393

Abstract: The present invention provides an adsorbent chip, which includes three components, a substrate, an intermediate layer of linker arms and an adsorbent film, which is attached to the linker arms. The adsorbent film is made up of a plurality of adsorbent particles, each of which includes a binding functionality. The invention also provides a method of making the chips of the invention in which the substrate-intermediate film cassette is formed and the adsorbent film is subsequently immobilized thereon. When the adsorbent film is from the same preparation across a particular batch of chips, the chips provide for the acquisition of data that are highly reproducible from one chip to the next throughout the particular batch of chips. Additionally, the invention provides methods for using the chips to perform assays.