公开(公告)号：US20160016992A1

公开(公告)日：2016-01-21

申请号：US14747029

申请日：2015-06-23

Applicant: EMD Millipore Corporation

Inventor： Nanying Bian ,  Christopher Gillespie ,  Matthew T. Stone ,  Mikhail Kozlov ,  Jie Chen ,  Martin Siwak

IPC: C07K1/36 ,  B01J41/20 ,  B01J39/26 ,  B01J20/20

CPC classification number: C07K1/36 ,  B01D15/125 ,  B01D15/1871 ,  B01D15/362 ,  B01D15/363 ,  B01D15/3809 ,  B01J20/20 ,  B01J20/282 ,  B01J39/26 ,  B01J41/20 ,  B01J43/00 ,  B01J47/04 ,  C07K1/16 ,  C07K1/20 ,  C07K16/00 ,  C07K2317/10

Abstract: The present invention provides novel and improved protein purification processes which incorporate certain types of carbonaceous materials and result in effective and selective removal of certain undesirable impurities without adversely effecting the yield of the desired protein product.

Abstract translation: 本发明提供新颖和改进的蛋白质纯化方法，其包含某些类型的碳质材料，并且导致有效和选择性地除去某些不需要的杂质，而不会不利地影响所需蛋白质产物的产率。

公开(公告)号：US20180362595A1

公开(公告)日：2018-12-20

申请号：US16113403

申请日：2018-08-27

Applicant: EMD Millipore Corporation

Inventor： Nanying Bian ,  Neil Soice ,  Shari Spector ,  Kara Levine

IPC: C07K14/31 ,  B01J20/32 ,  B01D15/38 ,  B01J20/286

Abstract: The present invention relates to chromatography ligands having improved caustic stability, e.g., ligands based on immunoglobulin-binding proteins such as, Staphylococcal protein A, as well as methods of making and using such ligands.

公开(公告)号：US09587009B2

公开(公告)日：2017-03-07

申请号：US14325014

申请日：2014-07-07

Applicant: EMD Millipore Corporation

Inventor： Nanying Bian ,  Senthil Ramaswamy ,  Neil Soice ,  Chen Wang ,  Yuan Wong

IPC: G01N33/552 ,  C07K16/00 ,  G01N33/538 ,  B01J20/10 ,  B01J20/28 ,  B01J20/281 ,  B01J20/283 ,  B01J20/286 ,  C03C11/00 ,  C03C12/00 ,  C03C17/28 ,  C07K1/22

CPC classification number: C07K16/00 ,  B01J20/103 ,  B01J20/28004 ,  B01J20/28014 ,  B01J20/28016 ,  B01J20/28026 ,  B01J20/28054 ,  B01J20/28085 ,  B01J20/281 ,  B01J20/283 ,  B01J20/286 ,  C03C11/00 ,  C03C12/00 ,  C03C17/28 ,  C07K1/22 ,  G01N33/538 ,  Y10T428/2982

Abstract: The invention relates generally to solid supports for chromatography. In specific embodiments the invention provides for solid supports suitable for affinity chromatography along with methods, systems and kits which use the same.

Abstract translation: 本发明一般涉及用于色谱的固体支持物。 在具体实施方案中，本发明提供了适用于亲和层析的固体支持物，以及使用其的方法，系统和试剂盒。

公开(公告)号：US20160193633A1

公开(公告)日：2016-07-07

申请号：US14912440

申请日：2014-06-19

Applicant: EMD MILLIPORE CORPORATION

Inventor： Nanying Bian ,  Sapna Mehtani ,  John Dana Hubbard

IPC: B08B9/027 ,  B08B3/08 ,  A61L2/18

CPC classification number: B08B9/027 ,  A61L2/18 ,  B08B3/08 ,  C07K1/22

Abstract: The present invention provides methods for cleaning a Protein A chromatography column employing a media comprising a Protein A ligand derived from the C domain of Staphylococcus aureus, such that the column can be cleaned using both acidic and alkaline solutions.

Abstract translation: 本发明提供使用包含衍生自金黄色葡萄球菌C结构域的蛋白A配体的培养基来清洗蛋白A色谱柱的方法，使得可以使用酸性和碱性溶液清洗该柱。

公开(公告)号：US20150252085A1

公开(公告)日：2015-09-10

申请号：US14683738

申请日：2015-04-10

Applicant: EMD Millipore Corporation

Inventor： Shari Spector ,  Robert Smith ,  Joe Orlando ,  Nanying Bian

IPC: C07K14/31 ,  C07K16/12 ,  C07K1/22

CPC classification number: C07K14/31 ,  B01D15/3809 ,  B01J20/286 ,  B01J20/3272 ,  B01J20/3274 ,  C07K1/22 ,  C07K16/1271 ,  C07K2319/21

Abstract: The present invention relates to chromatography matrices including ligands based on one or more domains of immunoglobulin-binding proteins such as, Staphylococcus aureus Protein A (SpA), as well as methods of using the same.

公开(公告)号：US09018305B2

公开(公告)日：2015-04-28

申请号：US14534999

申请日：2014-11-06

Applicant: EMD Millipore Corporation

Inventor： Shari Spector ,  Robert Smith ,  Joe Orlando ,  Nanying Bian

IPC: B01J20/286 ,  C07K1/22 ,  C07K14/31 ,  C07K16/12

CPC classification number: C07K14/31 ,  B01D15/3809 ,  B01J20/286 ,  B01J20/3272 ,  B01J20/3274 ,  C07K1/22 ,  C07K16/1271 ,  C07K2319/21

Abstract: The present invention relates to chromatography matrices including ligands based on one or more domains of immunoglobulin-binding proteins such as, Staphylococcus aureus Protein A (SpA), as well as methods of using the same.

Abstract translation: 本发明涉及包括基于免疫球蛋白结合蛋白（例如金黄色葡萄球菌蛋白A（SpA））的一个或多个结构域的配体的色谱基质，以及使用它们的方法。

公开(公告)号：US11084851B2

公开(公告)日：2021-08-10

申请号：US16113403

申请日：2018-08-27

Applicant: EMD Millipore Corporation

Inventor： Nanying Bian ,  Neil Soice ,  Shari Spector ,  Kara Levine

IPC: C07K14/31 ,  B01D15/38 ,  B01J20/286 ,  B01J20/32

Abstract: The present invention relates to chromatography ligands having improved caustic stability, e.g., ligands based on immunoglobulin-binding proteins such as, Staphylococcal protein A, as well as methods of making and using such ligands.

公开(公告)号：US10287314B2

公开(公告)日：2019-05-14

申请号：US14747029

申请日：2015-06-23

Applicant: EMD Millipore Corporation

Inventor： Nanying Bian ,  Christopher Gillespie ,  Matthew T. Stone ,  Mikhail Kozlov ,  Jie Chen ,  Martin Siwak

IPC: B01D15/36 ,  B01D15/12 ,  B01D15/18 ,  B01D15/38 ,  B01J39/26 ,  B01J41/20 ,  C07K1/36 ,  C07K1/16 ,  B01J20/282 ,  C07K1/20 ,  B01J43/00 ,  B01J47/04 ,  C07K16/00 ,  B01J20/20

Abstract: The present invention provides novel and improved protein purification processes which incorporate certain types of carbonaceous materials and result in effective and selective removal of certain undesirable impurities without adversely effecting the yield of the desired protein product.

公开(公告)号：US09376474B1

公开(公告)日：2016-06-28

申请号：US14970610

申请日：2015-12-16

Applicant: EMD Millipore Corporation

Inventor： Shari Spector ,  Robert Smith ,  Joe Orlando ,  Nanying Bian

IPC: C07K14/31 ,  C07K1/22 ,  C07K16/12

CPC classification number: C07K14/31 ,  B01D15/3809 ,  B01J20/286 ,  B01J20/3272 ,  B01J20/3274 ,  C07K1/22 ,  C07K16/1271 ,  C07K2319/21

Abstract: The present invention relates to chromatography matrices including ligands based on one or more domains of immunoglobulin-binding proteins such as, Staphylococcus aureus Protein A (SpA), as well as methods of using the same.

公开(公告)号：US09234010B2

公开(公告)日：2016-01-12

申请号：US14683738

申请日：2015-04-10

Applicant: EMD Millipore Corporation

Inventor： Shari Spector ,  Robert Smith ,  Joe Orlando ,  Nanying Bian

IPC: C07K14/31 ,  C07K1/22 ,  C07K16/12 ,  B01D15/38 ,  B01J20/286 ,  B01J20/32

CPC classification number: C07K14/31 ,  B01D15/3809 ,  B01J20/286 ,  B01J20/3272 ,  B01J20/3274 ,  C07K1/22 ,  C07K16/1271 ,  C07K2319/21

Abstract: The present invention relates to chromatography matrices including ligands based on one or more domains of immunoglobulin-binding proteins such as, Staphylococcus aureus Protein A (SpA), as well as methods of using the same.

Abstract translation: 本发明涉及包括基于免疫球蛋白结合蛋白（例如金黄色葡萄球菌蛋白A（SpA））的一个或多个结构域的配体的色谱基质，以及使用它们的方法。