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    Perhydrolase providing improved specific activity
    3.
    发明授权
    Perhydrolase providing improved specific activity 失效
    过水解酶提供更好的比活性

    公开(公告)号：US08389255B2

    公开(公告)日：2013-03-05

    申请号：US13070135

    申请日：2011-03-23

    申请人： Robert Dicosimo ,  John Edward Gavagan ,  Mark Scott Payne

    发明人： Robert Dicosimo ,  John Edward Gavagan ,  Mark Scott Payne

    IPC分类号： C12N9/18

    CPC分类号： C12N9/18 ,  C11D3/38636 ,  C12P7/40 ,  C12P7/54

    摘要： An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritima acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in specific activity. The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.

    摘要翻译： 提供具有过水解活性的乙酰木聚糖酯酶变体，用于从羧酸酯和过氧源产生过氧羧酸。 更具体地，使用易错PCR和定点诱变来修饰热转录海马乙酰木聚糖酯酶基因，以产生特征在于比活性增加的酶催化剂。 变体乙酰木聚糖酯酶可用于制备适用于各种用途的过氧羧酸，例如清洁，消毒，消毒，漂白，木浆加工和纸浆加工应用。

    PERHYDROLASE VARIANT PROVIDING IMPROVED SPECIFIC ACTIVITY
    5.
    发明申请
    PERHYDROLASE VARIANT PROVIDING IMPROVED SPECIFIC ACTIVITY 失效
    PERHYDROLASE变体提供改进的特定活性

    公开(公告)号：US20120156162A1

    公开(公告)日：2012-06-21

    申请号：US13328418

    申请日：2011-12-16

    申请人： Robert Dicosimo ,  Mark Scott Payne ,  John Edward Gavagan

    发明人： Robert Dicosimo ,  Mark Scott Payne ,  John Edward Gavagan

    IPC分类号： A61L9/01 ,  C12N9/16 ,  C12N1/21 ,  D21H21/32 ,  A01N37/02 ,  C07H21/04 ,  D06L3/00 ,  C12P7/62 ,  C12N15/63

    CPC分类号： C12N9/18 ,  C12Y301/01072

    摘要： An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritime acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in specific activity. The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.

    摘要翻译： 提供具有过水解活性的乙酰木聚糖酯酶变体，用于从羧酸酯和过氧源产生过氧羧酸。 更具体地，使用易错PCR和定点诱变来修饰Thermotoga海洋乙酰木聚糖酯酶基因，以产生特征在于比活性增加的酶催化剂。 变体乙酰木聚糖酯酶可用于制备适用于各种用途的过氧羧酸，例如清洁，消毒，消毒，漂白，木浆加工和纸浆加工应用。

    Immobilized microbial nitrilase for production of glycolic acid
    9.
    发明授权
    Immobilized microbial nitrilase for production of glycolic acid 有权
    用于生产乙醇酸的固定化微生物腈水解酶

    公开(公告)号：US07695945B2

    公开(公告)日：2010-04-13

    申请号：US11930550

    申请日：2007-10-31

    申请人： Robert Dicosimo ,  Arie Ben-Bassat

    发明人： Robert Dicosimo ,  Arie Ben-Bassat

    IPC分类号： C12N9/96 ,  C12P21/06 ,  C12N9/00 ,  C12N9/78 ,  C07H21/04

    CPC分类号： C12N9/78 ,  C12N11/06 ,  C12N11/10 ,  C12P7/42 ,  Y02P20/584 ,  Y02P20/588

    摘要： The present invention provides a process for preparing an enzyme catalyst having nitrilase activity for hydrolysis of glycolonitrile to glycolic acid with improved retention of recovered catalyst activity in consecutive batch reactions with catalyst recycle, said process comprising pretreating the enzyme catalyst with glutaraldehyde. The glutaraldehyde-pretreated enzyme catalyst has improved specific activity when compared to non-glutaraldehyde-pretreated enzyme catalysts, and thereby, has improved overall catalyst activity and productivity.

    摘要翻译： 本发明提供一种制备具有腈水解酶活性的酶催化剂的方法，所述酶催化剂具有乙醇腈水解为乙醇酸的酶催化剂，其具有在催化剂再循环的连续间歇反应中具有回收的催化剂活性的保持性，所述方法包括用戊二醛预处理酶催化剂。 与非戊二醛预处理的酶催化剂相比，戊二醛预处理的酶催化剂具有改善的比活性，从而改善了总的催化剂活性和生产率。

    IMMOBILIZED MICROBIAL NITRILASE FOR PRODUCTION OF GLYCOLIC ACID
    10.
    发明申请
    IMMOBILIZED MICROBIAL NITRILASE FOR PRODUCTION OF GLYCOLIC ACID 有权
    用于生产甘油酸的稳定的微生物硝酸盐

    公开(公告)号：US20090111158A1

    公开(公告)日：2009-04-30

    申请号：US11930550

    申请日：2007-10-31

    申请人： Robert Dicosimo ,  Arie Ben-Bassat

    发明人： Robert Dicosimo ,  Arie Ben-Bassat

    IPC分类号： C12N9/96

    CPC分类号： C12N9/78 ,  C12N11/06 ,  C12N11/10 ,  C12P7/42 ,  Y02P20/584 ,  Y02P20/588

    摘要： The present invention provides a process for preparing an enzyme catalyst having nitrilase activity for hydrolysis of glycolonitrile to glycolic acid with improved retention of recovered catalyst activity in consecutive batch reactions with catalyst recycle, said process comprising pretreating the enzyme catalyst with glutaraldehyde. The glutaraldehyde-pretreated enzyme catalyst has improved specific activity when compared to non-glutaraldehyde-pretreated enzyme catalysts, and thereby, has improved overall catalyst activity and productivity.

    摘要翻译： 本发明提供一种制备具有腈水解酶活性的酶催化剂的方法，所述酶催化剂具有乙醇腈水解为乙醇酸的酶催化剂，其具有在催化剂再循环的连续间歇反应中具有回收的催化剂活性的保持性，所述方法包括用戊二醛预处理酶催化剂。 与非戊二醛预处理的酶催化剂相比，戊二醛预处理的酶催化剂具有改善的比活性，从而改善了总的催化剂活性和生产率。