SYSTEM FOR OPTICAL STIMULATION OF TARGET CELLS
    1.
    发明申请
    SYSTEM FOR OPTICAL STIMULATION OF TARGET CELLS 审中-公开
    目标细胞光刺激系统

    公开(公告)号:US20100190229A1

    公开(公告)日:2010-07-29

    申请号:US12522528

    申请日:2008-01-09

    IPC分类号: C12N13/00 C12N5/079

    摘要: Stimulation of target cells using light, e.g., in vivo, is implemented using a variety of methods and devices. In one example, embodiments involve methods for stimulating target cells using a photosensitive protein that allows the target cells to be stimulated in response to light. In another specific example embodiment, target cells are stimulated using an implantable arrangement. The arrangement includes an electrical light-generation means for generating light and a biological portion. The biological portion has a photosensitive bio-molecular arrangement that responds to the generated light by stimulating target cells in vivo. Other aspects and embodiments are directed to systems and methods for screening chemicals based screening chemicals to identify their effects on cell membrane ion channels and pumps, and to systems and methods for controlling an action potential of neuron (e.g., in vivo and in vitro environments).

    摘要翻译: 使用光(例如,体内)使用各种方法和装置来实现靶细胞的刺激。 在一个实例中,实施方案涉及使用允许靶细胞响应于光而被刺激的光敏蛋白刺激靶细胞的方法。 在另一具体示例性实施方案中,使用可植入装置刺激靶细胞。 该装置包括用于产生光和生物部分的电光产生装置。 生物部分具有通过在体内刺激靶细胞来响应所产生的光的感光生物分子结构。 其他方面和实施方案涉及用于筛选基于化学品的筛选化学品以识别其对细胞膜离子通道和泵的影响的系统和方法,以及用于控制神经元的动作电位(例如,体内和体外环境)的系统和方法, 。

    CELL LINE, SYSTEM AND METHOD FOR OPTICAL-BASED SCREENING OF ION-CHANNEL MODULATORS
    5.
    发明申请
    CELL LINE, SYSTEM AND METHOD FOR OPTICAL-BASED SCREENING OF ION-CHANNEL MODULATORS 有权
    细胞线,系统和方法用于离子通道调制器的光学筛选

    公开(公告)号:US20090099038A1

    公开(公告)日:2009-04-16

    申请号:US12187927

    申请日:2008-08-07

    摘要: A variety of applications, systems, methods and constructs are implemented for use in connection with screening of ion-channel modulators. Consistent with one such system, drug candidates are screened to identify their effects on cell membrane ion channels and pumps. The system includes screening cells having light responsive membrane ion switches, voltage-gated ion switches and fluorescence producing voltage sensors. A chemical delivery device introduces the drug candidates to be screened. An optical delivery device activates the light responsive ion switches. An optical sensor monitors fluorescence produced by the voltage sensors. A processor processes data received from the optical sensor. A memory stores the data received from the optical sensor.

    摘要翻译: 实现了各种应用,系统,方法和结构,用于与离子通道调制器的筛选相结合。 与一个这样的系统一致,筛选候选药物以鉴定其对细胞膜离子通道和泵的影响。 该系统包括筛选具有光响应性膜离子开关,电压门控离子开关和荧光产生电压传感器的电池。 化学品输送装置引入待筛选的候选药物。 光学传递装置激活光响应离子开关。 光学传感器监测由电压传感器产生的荧光。 处理器处理从光学传感器接收的数据。 存储器存储从光学传感器接收的数据。

    Screening test drugs to identify their effects on cell membrane voltage-gated ion channel
    6.
    发明授权
    Screening test drugs to identify their effects on cell membrane voltage-gated ion channel 有权
    筛选试验药物,以鉴别其对细胞膜电压门控离子通道的影响

    公开(公告)号:US09274099B2

    公开(公告)日:2016-03-01

    申请号:US12187927

    申请日:2008-08-07

    摘要: A variety of applications, systems, methods and constructs are implemented for use in connection with screening of ion-channel modulators. Consistent with one such system, drug candidates are screened to identify their effects on cell membrane ion channels and pumps. The system includes screening cells having light responsive membrane ion switches, voltage-gated ion switches and fluorescence producing voltage sensors. A chemical delivery device introduces the drug candidates to be screened. An optical delivery device activates the light responsive ion switches. An optical sensor monitors fluorescence produced by the voltage sensors. A processor processes data received from the optical sensor. A memory stores the data received from the optical sensor.

    摘要翻译: 实现了各种应用,系统,方法和结构,用于与离子通道调制器的筛选相结合。 与一个这样的系统一致,筛选候选药物以鉴定其对细胞膜离子通道和泵的影响。 该系统包括筛选具有光响应性膜离子开关,电压门控离子开关和荧光产生电压传感器的电池。 化学品输送装置引入待筛选的候选药物。 光学传递装置激活光响应离子开关。 光学传感器监测由电压传感器产生的荧光。 处理器处理从光学传感器接收的数据。 存储器存储从光学传感器接收的数据。

    APPARATUS AND METHODS FOR CONTROLLING CELLULAR DEVELOPMENT
    9.
    发明申请
    APPARATUS AND METHODS FOR CONTROLLING CELLULAR DEVELOPMENT 审中-公开
    用于控制细胞发育的装置和方法

    公开(公告)号:US20110159562A1

    公开(公告)日:2011-06-30

    申请号:US12997140

    申请日:2009-06-17

    IPC分类号: C12N13/00

    摘要: According to one aspect and example, a method for facilitating cellular interactions in biological tissue provides controllable activation of a selected type of stem cell among a plurality of cell types present in the tissue. The method includes various steps including the introduction of a microbial opsin into a region of the tissue that includes a selected type of stem cell, by expressing the microbial opsin in the stem cell. A light source is then introduced near the stem cell, and the light source is used to controllably activate the light source to direct pulses of illumination from the light source to the selected type of stem cell, for selectively controlling the growth and development of the stem cell in a manner that is independent of the growth and development of the other types of cells.

    摘要翻译: 根据一个方面和实施例,促进生物组织中的细胞相互作用的方法提供组织中存在的多种细胞类型中所选类型的干细胞的可控活化。 该方法包括通过在干细胞中表达微生物视蛋白来将微生物视蛋白引入到包括选定类型的干细胞的组织的区域中的各种步骤。 然后在干细胞附近引入光源,并且光源用于可控制地激活光源以将来自光源的照明脉冲引导到选定类型的干细胞,用于选择性地控制茎的生长和发育 细胞以独立于其他类型细胞的生长和发育的方式。

    Implantable optical stimulators
    10.
    发明授权

    公开(公告)号:US10434327B2

    公开(公告)日:2019-10-08

    申请号:US12263044

    申请日:2008-10-31

    IPC分类号: A61N5/06

    摘要: Various systems and methods are implemented for in vivo use in a living animal. One such method involves stimulating target cells having light-responsive proteins and includes providing an elongated light-delivery structure in a narrow passageway in the animal, the elongated light-delivery structure having separately-activatable light sources located along the length of the elongated light-delivery structure. The method also includes activating less than all the light sources to deliver light to light-responsive proteins adjacent to the activated light sources along the length of the elongated light-delivery structure, thereby stimulating target cells in vivo.