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    DNA sequencing methods and detectors and systems for carrying out the same
    5.
    发明授权
    DNA sequencing methods and detectors and systems for carrying out the same 有权
    DNA测序方法和检测器和系统进行相同

    公开(公告)号：US08969090B2

    公开(公告)日：2015-03-03

    申请号：US12984269

    申请日：2011-01-04

    申请人： Hongye Sun ,  Eric S. Nordman ,  Mark F. Oldham ,  John R. O'Neill ,  Charles Connell ,  Umberto Ulmanella ,  Aldrich N. K. Lau ,  Theofilos Kotseroglou ,  Kenneth J. Livak

    发明人： Hongye Sun ,  Eric S. Nordman ,  Mark F. Oldham ,  John R. O'Neill ,  Charles Connell ,  Umberto Ulmanella ,  Aldrich N. K. Lau ,  Theofilos Kotseroglou ,  Kenneth J. Livak

    IPC分类号： G01N27/447 ,  G01N33/543 ,  C12Q1/68 ,  G01N27/414 ,  G01Q60/24 ,  C08G69/42 ,  B01L3/00

    CPC分类号： C12Q1/6869 ,  B01L3/502761 ,  G01N27/3278 ,  G01N27/414 ,  G01N27/4145 ,  G01N27/4146 ,  G01N33/5438 ,  Y10S977/924 ,  Y10T436/143333 ,  C12Q2565/631

    摘要： In some embodiments, an analyte detection system is provided that includes a nanochannel, an electrode arrangement, and a plurality of nanoFET devices disposed in the nanochannel. A plurality of nucleic acid base detection components can be used that include a plurality of nanopores, a plurality of nanochannels, a plurality of hybridization probes, combinations thereof, and the like. According to other embodiments of the present teachings, different coded molecules are hybridized to a target DNA molecule and used to detect the presence of various sequences along the target molecule. A kit including mixtures of coded molecules is also provided. In some embodiments, devices including nanochannels, nanopores, and the like, are used for manipulating movement of DNA molecules, for example, in preparation for a DNA sequencing detection. Nanopore structures and methods of making the same are also provided as are methods of nucleic acid sequencing using the nanopore structures. Surface-modified nanopores are provided as are methods of making them. In some embodiments, surfaced-modified nanopores for slowing the translocation of single stranded DNA (ssDNA) through the nanopore are provided, as are nanopores configured to detect each of a plurality of different bases on an ssDNA strand.

    摘要翻译： 在一些实施例中，提供了分析物检测系统，其包括纳米通道，电极布置以及设置在纳米通道中的多个纳米器件器件。 可以使用多个核酸碱基检测组分，其包括多个纳米孔，多个纳米通道，多个杂交探针及其组合等。 根据本教导的其他实施方案，将不同的编码分子与靶DNA分子杂交并用于检测沿着靶分子的各种序列的存在。 还提供了包含编码分子混合物的试剂盒。 在一些实施方案中，包括纳米通道，纳米孔等的装置用于操纵DNA分子的移动，例如用于DNA测序检测的准备。 还提供了制备其的纳米孔结构及其制备方法，使用纳米孔结构的核酸测序方法也是如此。 提供表面改性的纳米孔是制备它们的方法。 在一些实施方案中，提供了用于减缓通过纳米孔的单链DNA（ssDNA）易位的表面改性的纳米孔，以及配置成检测ssDNA链上的多个不同碱基中的每一个的纳米孔。

    High speed, high resolution compositions, methods, and kits for capillary electrophoresis
    8.
    发明授权
    High speed, high resolution compositions, methods, and kits for capillary electrophoresis 有权
    用于毛细管电泳的高速，高分辨率组合物，方法和试剂盒

    公开(公告)号：US06706162B1

    公开(公告)日：2004-03-16

    申请号：US09668960

    申请日：2000-09-25

    申请人： Karl O. Voss ,  Aldrich N. K. Lau

    发明人： Karl O. Voss ,  Aldrich N. K. Lau

    IPC分类号： B01D5702

    CPC分类号： G01N27/44747 ,  B01D53/02 ,  G01N27/44791

    摘要： The invention provides compositions, methods and kits for high speed, high resolution of analytes by capillary electrophoresis starting with uncoated capillaries. The compositions comprise a sieving component, comprising a non-crosslinked acrylamide polymer, and a surface interaction component, comprising at least one uncharged and non-crosslinked water-soluble silica-adsorbing polymer. Methods for employing the novel compositions in capillary electrophoresis are provided. Kits comprising the novel compositions for use in the novel methods are also provided.

    摘要翻译： 本发明提供了用未经涂覆的毛细管从毛细管电泳开始高分辨率分析物的组合物，方法和试剂盒。 组合物包含筛分组分，其包含非交联的丙烯酰胺聚合物和包含至少一种不带电和非交联的水溶性二氧化硅吸附聚合物的表面相互作用组分。 提供了在毛细管电泳中使用新型组合物的方法。 还提供了包含用于新颖方法的新型组合物的试剂盒。