公开(公告)号：US20230124451A1

公开(公告)日：2023-04-20

申请号：US18076986

申请日：2022-12-07

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Khairuzzaman Bashar MULLAH ,  Brian EVANS ,  Scott C. BENSON ,  Chu-An CHANG ,  Xiongwei YAN

IPC: C12Q1/6818 ,  C12Q1/686

Abstract: Disclosed is a probe for use in biological assays. The probe includes a fluorescent dye bound to a quencher compound through an oligonucleotide linker. Also disclosed are methods of using the probe, such as for a polymerase chain reaction (PCR), such as in a quantitative PCR reaction (qPCR), as well as kits including the probe.

公开(公告)号：US20240110063A1

公开(公告)日：2024-04-04

申请号：US18345621

申请日：2023-06-30

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Andrew LEVITZ ,  Khairuzzaman Bashar MULLAH ,  Brian EVANS ,  Scott C. BENSON ,  Chris VONNEGUT

IPC: C09B57/14 ,  C12Q1/6818 ,  C12Q1/6844 ,  G01N29/24

CPC classification number: C09B57/14 ,  C12Q1/6818 ,  C12Q1/6844 ,  G01N29/2418

Abstract: The present disclosure relates to dibenzoxanthene compounds that are efficient quenchers of fluorescence, for example in the far red and near infrared spectrum. Applications using the dibenzoxanthene quenching compounds and methods of making same are also described.

公开(公告)号：US20160108079A1

公开(公告)日：2016-04-21

申请号：US14952149

申请日：2015-11-25

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Zhaochun MA ,  Khairuzzaman Bashar MULLAH ,  Robert G. EASON

IPC: C07H19/16

CPC classification number: C07H19/16 ,  C07D239/54 ,  C07D405/04 ,  C07D473/18 ,  C07D473/34 ,  C07H19/06 ,  C07H21/00 ,  C07H21/02 ,  C07H21/04 ,  C12Q1/6806

Abstract: Methods for preparation of 2′,3′-dideoxynucleotides support structures, such as 2′,3′-dideoxyguanosine, 2′,3′-dideoxyadenosine, and 3′-deoxythymidine support structures are disclosed. Various methods of using such structures are also provided, such as their use for automated DNA synthesis and pyrophosphorolysis activated polymerization.

Abstract translation: 公开了制备2'，3'-二脱氧核苷酸支持结构的方法，例如2'，3'-二脱氧鸟苷，2'，3'-二脱氧腺苷和3'-脱氧胸苷支持结构。 还提供了使用这些结构的各种方法，例如其用于自动DNA合成和焦磷酸裂解活化聚合的用途。

公开(公告)号：US20140170656A1

公开(公告)日：2014-06-19

申请号：US14137376

申请日：2013-12-20

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Muhammad A. SHARAF ,  Timothy WOUDENBERG ,  Khairuzzaman Bashar MULLAH

IPC: C12Q1/68

CPC classification number: C12Q1/6825 ,  C12Q1/6816 ,  C12Q1/6823 ,  C12Q1/6827 ,  C12Q1/6832 ,  C12Q1/6876 ,  G01N27/44726 ,  G01N27/44791 ,  C12Q2565/631

Abstract: The present disclosure relates to methods of identifying target nucleic acids by using coded molecules and its analysis by translocation through a nanopore. Generally, coded molecules are subject to a target polynucleotide dependent modification. The modified coded molecule is detected by isolating the modified coded molecules from the unmodified coded molecules prior to analysis through the nanopore or by detecting a change in the signal pattern of the coded molecule when analyzed through the nanopore.

Abstract translation: 本公开内容涉及通过使用编码分子鉴定靶核酸的方法及其通过纳米孔易位的分析。 通常，经编码的分子经受目标多核苷酸依赖性修饰。 通过在通过纳米孔分析之前将经修饰的编码分子与未修饰的编码分子分离，或者当通过纳米孔分析时，通过检测编码分子的信号图谱的变化来检测修饰的编码分子。