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公开(公告)号：US20110262949A1
公开(公告)日：2011-10-27
申请号：US12734226
申请日：2008-10-23
申请人： Mohammad R. Seyedsayamdost , Jianming Xie , Clement Tsz Chan , Lital Alfonta , Peter G. Schultz , Joanne Stubbe
发明人： Mohammad R. Seyedsayamdost , Jianming Xie , Clement Tsz Chan , Lital Alfonta , Peter G. Schultz , Joanne Stubbe
CPC分类号： C12N9/0093 , C12Q1/26 , G01N2333/9029
摘要： This invention provides reductase proteins that comprise NH2Y unnatural amino acid residues, systems of orthogonal elements for incorporating NH2Y into reductases and methods of using NH2Y amino acid residues in reductases as molecular probes for probing reductases function, structure and activity.
摘要翻译： 本发明提供了包含NH2Y非天然氨基酸残基的还原酶蛋白，用于将NH2Y并入还原酶的正交元件的系统和在还原酶中使用NH 2 Y氨基酸残基作为探针还原酶功能，结构和活性的分子探针的方法。
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公开(公告)号：US08673591B2
公开(公告)日：2014-03-18
申请号：US12734226
申请日：2008-10-23
申请人： Mohammad R. Seyedsayamdost , Jianming Xie , Clement Tsz Chan , Lital Alfonta , Peter G. Schultz , Joanne Stubbe
发明人： Mohammad R. Seyedsayamdost , Jianming Xie , Clement Tsz Chan , Lital Alfonta , Peter G. Schultz , Joanne Stubbe
IPC分类号： C12N15/67 , C12P21/02 , C07K14/00 , C07K14/505
CPC分类号： C12N9/0093 , C12Q1/26 , G01N2333/9029
摘要： This invention provides reductase proteins that comprise NH2Y unnatural amino acid residues, systems of orthogonal elements for incorporating NH2Y into reductases and methods of using NH2Y amino acid residues in reductases as molecular probes for probing reductases function, structure and activity.
摘要翻译： 本发明提供了包含NH2Y非天然氨基酸残基的还原酶蛋白，用于将NH2Y并入还原酶的正交元件的系统和在还原酶中使用NH 2 Y氨基酸残基作为探针还原酶功能，结构和活性的分子探针的方法。
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公开(公告)号：US20090208994A1
公开(公告)日：2009-08-20
申请号：US12257303
申请日：2008-10-23
申请人： Mohammad R. Seyedsayamdost , Jianming Xie , Tsz Yan Clement Chan , Lital Alfonta , Peter G. Schultz , Joanne Stubbe
发明人： Mohammad R. Seyedsayamdost , Jianming Xie , Tsz Yan Clement Chan , Lital Alfonta , Peter G. Schultz , Joanne Stubbe
CPC分类号： C12N9/0093 , C12Q1/26 , G01N2333/9029
摘要： This invention provides reductase proteins that comprise NH2Y unnatural amino acid residues, systems of orthogonal elements for incorporating NH2Y into reductases and methods of using NH2Y amino acid residues in reductases as molecular probes for probing reductases function, structure and activity.
摘要翻译： 本发明提供了包含NH2Y非天然氨基酸残基的还原酶蛋白，用于将NH2Y并入还原酶的正交元件的系统和在还原酶中使用NH 2 Y氨基酸残基作为探针还原酶功能，结构和活性的分子探针的方法。
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4.发明授权Orthogonal translation components for the in vivo incorporation of unnatural amino acids 有权用于体内引入非天然氨基酸的正交翻译组分公开(公告)号：US09102932B2
公开(公告)日：2015-08-11
申请号：US13492296
申请日：2012-06-08
申请人： Peter G. Schultz , Lital Alfonta , Johnathan R. Chittuluru , Alexander Deiters , Dan Groff , Daniel Summerer , Meng-Lin Tsao , Jiangyun Wang , Ning Wu , Jianming Xie , Huaqiang Zeng , Mohammad Seyedsayamdost , James Turner
发明人： Peter G. Schultz , Lital Alfonta , Johnathan R. Chittuluru , Alexander Deiters , Dan Groff , Daniel Summerer , Meng-Lin Tsao , Jiangyun Wang , Ning Wu , Jianming Xie , Huaqiang Zeng , Mohammad Seyedsayamdost , James Turner
CPC分类号： C12N9/93 , C12N15/1058 , C12P21/00 , C12P21/02
摘要： The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetase that can incorporate unnatural amino acid into proteins produced in eubacterial host cells such as E. coli, or in a eukaryotic host such as a yeast cell. The invention provides, for example but not limited to, novel orthogonal synthetases, methods for identifying and making the novel synthetases, methods for producing proteins containing unnatural amino acids, and translation systems.
摘要翻译： 本发明涉及tRNA和氨酰-tRNA合成酶的正交对，其可以将非天然氨基酸掺入在诸如大肠杆菌或真核宿主如酵母细胞中的细胞宿主细胞中产生的蛋白质中。 本发明提供了例如但不限于新的正交合成酶，用于鉴定和制备新合成酶的方法，用于产生含有非天然氨基酸的蛋白质的方法和翻译系统。
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5.发明申请Orthogonal Translation Components for the in Vivo Incorporation of Unnatural Amino Acids 有权用于体内并入非天然氨基酸的正交翻译组分公开(公告)号：US20080233611A1
公开(公告)日：2008-09-25
申请号：US11665083
申请日：2005-10-27
申请人： Peter G. Schultz , Lital Alfonta , Jonathan R. Chittuluru , Alexander Deiters , Dan Groff , Daniel Summerer , Meng-Lin Tsao , Jiangyun Wang , Ning Wu , Jianming Xie , Huaqiang Zeng
发明人： Peter G. Schultz , Lital Alfonta , Jonathan R. Chittuluru , Alexander Deiters , Dan Groff , Daniel Summerer , Meng-Lin Tsao , Jiangyun Wang , Ning Wu , Jianming Xie , Huaqiang Zeng
CPC分类号： C12N9/93 , C12N15/1058 , C12P21/00 , C12P21/02
摘要： The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetases that can incorporate unnatural amino acids into proteins produced in eubacterial host cells such as E. coli, or in a eukaryotic host such as a yeast cell. The invention provides, for example but not limited to, novel orthogonal synthetases, methods for identifying and making the novel synthetases, methods for producing proteins containing unnatural amino acids, and translation systems.
摘要翻译： 本发明涉及tRNA和氨酰-tRNA合成酶的正交对，其可以将非天然氨基酸掺入到在细菌宿主细胞如大肠杆菌或真核宿主如酵母细胞中产生的蛋白质中。 本发明提供了例如但不限于新的正交合成酶，用于鉴定和制备新合成酶的方法，用于产生含有非天然氨基酸的蛋白质的方法和翻译系统。
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6.发明申请Orthogonal Translation Components for the in Vivo Incorporation of Unnatural Amino Acids 审中-公开用于体内并入非天然氨基酸的正交翻译组分公开(公告)号：US20130244245A1
公开(公告)日：2013-09-19
申请号：US13492296
申请日：2012-06-08
申请人： Peter G. Schultz , Lital Alfonta , Johnathan R. Chittuluru , Alexander Deiters , Dan Groff , Daniel Summerer , Meng-Lin Tsao , Jiangyun Wang , Ning Wu , Jianming Xie , Huaqiang Zeng , Mohammad Seyedsayamdost , James Turner
发明人： Peter G. Schultz , Lital Alfonta , Johnathan R. Chittuluru , Alexander Deiters , Dan Groff , Daniel Summerer , Meng-Lin Tsao , Jiangyun Wang , Ning Wu , Jianming Xie , Huaqiang Zeng , Mohammad Seyedsayamdost , James Turner
CPC分类号： C12N9/93 , C12N15/1058 , C12P21/00 , C12P21/02
摘要： The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetase that can incorporate unnatural amino acid into proteins produced in eubacterial host cells such as E. coli, or in a eukaryotic host such as a yeast cell. The invention provides, for example but not limited to, novel orthogonal synthetases, methods for identifying and making the novel synthetases, methods for producing proteins containing unnatural amino acids, and translation systems.
摘要翻译： 本发明涉及tRNA和氨酰-tRNA合成酶的正交对，其可以将非天然氨基酸掺入在诸如大肠杆菌或真核宿主如酵母细胞中的细胞宿主细胞中产生的蛋白质中。 本发明提供了例如但不限于新的正交合成酶，用于鉴定和制备新合成酶的方法，用于产生含有非天然氨基酸的蛋白质的方法和翻译系统。
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7.发明授权Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells 有权在哺乳动物细胞中将5-羟色氨酸选择性掺入蛋白质公开(公告)号：US08658395B2
公开(公告)日：2014-02-25
申请号：US13546582
申请日：2012-07-11
申请人： Zhiwen Zhang , Lital Alfonta , Peter G. Schultz
发明人： Zhiwen Zhang , Lital Alfonta , Peter G. Schultz
摘要： This invention provides methods and compositions for incorporation of an unnatural amino acid into a peptide using an orthogonal aminoacyl tRNA synthetase/tRNA pair. In particular, an orthogonal pair is provided to incorporate 5-hydroxy-L-tryptophan in a position encoded by an opal mutation.
摘要翻译： 本发明提供了使用正交氨酰基tRNA合成酶/ tRNA对将非天然氨基酸并入肽的方法和组合物。 特别地，提供了正交对以在由蛋白石突变编码的位置中并入5-羟基-L-色氨酸。
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8.发明授权公开(公告)号：US08008041B2
公开(公告)日：2011-08-30
申请号：US10575991
申请日：2004-10-13
申请人： Lital Alfonta , Peter G. Schultz , Zhiwen Zhang
发明人： Lital Alfonta , Peter G. Schultz , Zhiwen Zhang
摘要： Compositions and methods of producing components of protein biosynthetic machinery that include orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of tRNAs/synthetases, which incorporate redox active amino acids into proteins are provided. Methods for identifying these orthogonal pairs are also provided along with methods of producing proteins with redox active amino acids using these orthogonal pairs.
摘要翻译： 提供了将氧化还原活性氨基酸掺入蛋白质的蛋白质生物合成机理组分的组成和方法，其中包括正交tRNA，正交氨酰-tRNA合成酶和tRNA /合成酶正交对。 还提供了用于鉴定这些正交对的方法以及使用这些正交对产生具有氧化还原活性氨基酸的蛋白质的方法。
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9.发明授权公开(公告)号：US07811801B2
公开(公告)日：2010-10-12
申请号：US12317034
申请日：2008-12-17
申请人： Lital Alfonta , Peter G. Schultz , Zhiwen Zhang
发明人： Lital Alfonta , Peter G. Schultz , Zhiwen Zhang
摘要： Compositions and methods of producing components of protein biosynthetic machinery that include orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of tRNAs/synthetases, which incorporate redox active amino acids into proteins are provided. Methods for identifying these orthogonal pairs are also provided along with methods of producing proteins with redox active amino acids using these orthogonal pairs.
摘要翻译： 提供了将氧化还原活性氨基酸掺入蛋白质的蛋白质生物合成机理组分的组成和方法，其中包括正交tRNA，正交氨酰-tRNA合成酶和tRNA /合成酶正交对。 还提供了用于鉴定这些正交对的方法以及使用这些正交对产生具有氧化还原活性氨基酸的蛋白质的方法。
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公开(公告)号：US20110008782A1
公开(公告)日：2011-01-13
申请号：US12806751
申请日：2010-08-20
申请人： Lital Alfonta , Peter G. Schultz , Zhiwen Zhang
发明人： Lital Alfonta , Peter G. Schultz , Zhiwen Zhang
摘要： Compositions and methods of producing components of protein biosynthetic machinery that include orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of tRNAs/synthetases, which incorporate redox active amino acids into proteins are provided. Methods for identifying these orthogonal pairs are also provided along with methods of producing proteins with redox active amino acids using these orthogonal pairs.
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