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公开(公告)号：US11027243B2
公开(公告)日：2021-06-08
申请号：US15748988
申请日：2016-07-28
Applicant: North Carolina State University
Inventor： Michael Leonard Heller , Ruben G. Carbonell , Behnam Pourdeyhimi
IPC: B01D15/36 , B01D67/00 , B01D69/02 , B01D71/40 , B01D71/48 , B01D71/78 , B01D71/82 , B01J39/19 , B01D69/10 , B01J41/13 , C07K1/18 , D01F8/14 , D04H1/435 , D04H1/4382 , D04H3/011 , D04H3/016 , D06M13/203 , D06M14/10 , D06M14/28 , D06M15/273 , D06M101/32
Abstract: The invention provides polymer-grafted and functionalized nonwoven membranes adapted for use in bioseparation processes, the membranes including a nonwoven web of polyester fibers having an average fiber diameter of less than about 1.5 microns, each of the plurality of polyester fibers having grafted thereon a plurality of polymer segments constructed of a methacrylate polymer, each polymer segment carrying a functional group adapted for binding to a target molecule. The invention also provides a method of bioseparation comprising passing a solution comprising the target molecule, such as a protein, through the nonwoven membrane of the invention such that at least a portion of the target molecule in the solution binds to the nonwoven membrane. A method for preparing a polymer-grafted and functionalized nonwoven membrane adapted for use in bioseparation processes is also provided.
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公开(公告)号：US20250092089A1
公开(公告)日：2025-03-20
申请号：US18890289
申请日：2024-09-19
Applicant: North Carolina State University , Merck Sharp and Dohme LLC , EMD Millipore Corporation , Rensselaer Polytechnic Institute
Inventor： Stefano Menegatti , Ruben G. Carbonell , Jacob P. LeBarre , Cristiana Boi , Steven Cramer , Michael Phillips , David Roush
Abstract: The present disclosure provides methods related to the purification of a biologic. In particular, the present disclosure methods for purifying a biologic using a multifunctional chromatography medium comprising porous beads or particles having a defined pore diameter distribution and one or more ligands conjugated to the surface of the pores capable of removing process-related impurities and product-related impurities from a target biologic.
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公开(公告)号：US10266566B2
公开(公告)日：2019-04-23
申请号：US14904715
申请日：2014-07-15
Applicant: North Carolina State University
Inventor： Stefano Menegatti , Benjamin G. Bobay , Ruben G. Carbonell
Abstract: This invention relates generally to the discovery of novel protease-resistant peptide ligands and uses thereof. Specifically, the present invention provides a protease-resistant peptide with three to twenty amino acids capable of binding a biological and comprising one or more basic amino acid(s) and I or aromatic amino acids, wherein one or more of the amino acids is substituted with a non-naturally occurring amino acid analog.
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公开(公告)号：US20160159859A1
公开(公告)日：2016-06-09
申请号：US14904715
申请日：2014-07-15
Applicant: NORTH CAROLINA STATE UNIVERSITY
Inventor： Stefano Menegatti , Benjamin G. Bobay , Ruben G. Carbonell
CPC classification number: C07K7/06 , C07K1/22 , C07K7/08 , C07K16/065
Abstract: This invention relates generally to the discovery of novel protease-resistant peptide ligands and uses thereof. Specifically, the present invention provides a protease-resistant peptide with three to twenty amino acids capable of binding a biological and comprising one or more basic amino acid(s) and I or aromatic amino acids, wherein one or more of the amino acids is substituted with a non-naturally occurring amino acid analog.
Abstract translation: 本发明一般涉及新蛋白酶抗性肽配体的发现及其用途。 具体地说，本发明提供一种能够结合生物学并含有一个或多个碱性氨基酸和I或芳族氨基酸的3-20个氨基酸的蛋白酶抗性肽，其中一个或多个氨基酸被取代 与非天然存在的氨基酸类似物。
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公开(公告)号：US20150284889A1
公开(公告)日：2015-10-08
申请号：US14746529
申请日：2015-06-22
Inventor： Yong Zheng , Sumana Roy Chowdhury , Patrick Vasconcelos Gurgel , Haiyan Liu , Ruben G. Carbonell
CPC classification number: D04H1/56 , D04H1/42 , D04H1/565 , D04H1/587 , D04H1/64 , D04H1/641 , D06M10/001 , D06M10/025 , D06M14/22 , D06M14/26 , D06M14/28 , D06M14/32 , D06M14/34 , Y10T442/20 , Y10T442/277 , Y10T442/2861 , Y10T442/2877 , Y10T442/2885 , Y10T442/2893 , Y10T442/2902 , Y10T442/291 , Y10T442/2918 , Y10T442/60 , Y10T442/659
Abstract: The present invention describes a novel process for the conformal coating of polymer fibers on nonwoven substrates. This process is based on the modification of polymer fiber surfaces by controlling the degree of etching and oxidation, which improves adhesion of initiators to the surface and facilitates subsequent conformal polymer grafting. The modified fiber surfaces render new functionalities to the surface, such as increasing hydrophilicity, attaching ligands or changing surface energy. The invention includes the modified polymer fibers produced by the process described herein.
Abstract translation: 本发明描述了一种用于在非织造衬底上共聚涂覆聚合物纤维的新方法。 该方法基于通过控制蚀刻和氧化的程度来改进聚合物纤维表面，这改善了引发剂对表面的粘合性，并有助于随后的共形聚合物接枝。 改性纤维表面赋予表面新功能，例如增加亲水性，附着配体或改变表面能。 本发明包括通过本文所述方法生产的改性聚合物纤维。
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Patent Agency Ranking
公开(公告)号：US20190284321A1
公开(公告)日：2019-09-19
申请号：US16318649
申请日：2017-06-17
Applicant: North Carolina State University
Inventor： Michael Leonard Heller , Ruben G. Carbonell
IPC: C08F283/02 , B01J41/20 , B01J41/14 , B01J41/13 , B01J39/26 , B01J39/19 , B01J39/20 , C07K1/18 , B01D71/78 , B01D71/26 , B01D71/40 , B01D67/00 , B01D15/20 , B01D15/36
Abstract: The invention provides methods for preparing a polymer-grafted and functionalized nonwoven membrane adapted for use in separation processes. The invention further provides so-formed membranes as well as improved separation methods utilizing the membranes. The polymer-grafted and functionalized nonwoven membranes are particularly formed utilizing thermal grafting. In particular, an acrylate or methacrylate polymer can be grafted onto a nonwoven web comprising a plurality of polymeric fibers to form a plurality of polymer segments covalently attached to the polymeric fibers. Thermal grafting particularly can comprise using a thermal initiator and exposing the nonwoven web to heat to initiate polymerization of the acrylate or methacrylate monomer. The grafted polymeric fibers can be functionalized to attach at least one functional group adapted for binding to a target molecule to the polymer segments of the grafted polymeric fibers.
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公开(公告)号：US09162223B2
公开(公告)日：2015-10-20
申请号：US13724245
申请日：2012-12-21
Applicant: NORTH CAROLINA STATE UNIVERSITY
Inventor： Ruben G. Carbonell , Stefano Menegatti , Amith D. Naik
IPC: B01J39/26 , B01J20/286 , B01D15/38 , B01J20/32
CPC classification number: B01J39/26 , B01D15/3804 , B01J20/286 , B01J20/3251 , B01J20/3272
Abstract: This invention is directed to the production of improved chromatographic resins and method of making and using such resins.
Abstract translation: 本发明涉及改进色谱树脂的制备及其制备和使用方法。
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公开(公告)号：US20140206021A1
公开(公告)日：2014-07-24
申请号：US14069052
申请日：2013-10-31
Inventor： David J. Hammond , Julia T. Lathrop , Larisa Cervenakova , Ruben G. Carbonell
IPC: G01N33/68
CPC classification number: G01N33/6896 , C07K5/0808 , C07K5/081 , C07K5/0812 , C07K5/0819 , C07K5/0821 , C07K14/47 , G01N2800/2828
Abstract: Ligands that bind to prion proteins and methods for using the ligands for detecting or removing a prion protein from a sample, such as a biological fluid or an environmental sample. The ligands are capable of binding to one or more forms of prion protein including cellular prion protein (PrPc), infectious prion protein (PrPsc), and recombinant prion protein (PrPr). Prions from various species, including humans and hamsters, are bound by the ligands. Also provided is a method of treating or retarding the development of a prion-associated pathology in a subject
Abstract translation: 与朊病毒蛋白结合的配体和使用配体检测或从样品例如生物液体或环境样品中除去朊病毒蛋白的方法。 配体能够结合一种或多种形式的朊病毒蛋白，包括细胞朊蛋白（PrPc），感染性朊病毒蛋白（PrPsc）和重组朊蛋白（PrPr）。 来自各种物种的朊病毒，包括人类和仓鼠，都被配体结合。 还提供了治疗或延缓受试者中朊病毒相关病理学发展的方法
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公开(公告)号：US20190001281A1
公开(公告)日：2019-01-03
申请号：US15748988
申请日：2016-07-28
Applicant: North Carolina State University
Inventor： Michael Leonard Heller , Ruben G. Carbonell , Behnam Pourdeyhimi
IPC: B01D71/82 , B01D71/48 , B01D71/78 , B01D71/40 , B01D67/00 , B01D69/02 , B01D15/36 , D04H1/435 , D04H1/4382 , D04H3/011 , D04H3/016 , D01F8/14 , C07K1/18 , B01J39/19 , B01J41/13
Abstract: The invention provides polymer-grafted and functionalized nonwoven membranes adapted for use in bioseparation processes, the membranes including a nonwoven web of polyester fibers having an average fiber diameter of less than about 1.5 microns, each of the plurality of polyester fibers having grafted thereon a plurality of polymer segments constructed of a methacrylate polymer, each polymer segment carrying a functional group adapted for binding to a target molecule. The invention also provides a method of bioseparation comprising passing a solution comprising the target molecule, such as a protein, through the nonwoven membrane of the invention such that at least a portion of the target molecule in the solution binds to the nonwoven membrane. A method for preparing a polymer-grafted and functionalized nonwoven membrane adapted for use in bioseparation processes is also provided.
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公开(公告)号：US09678085B2
公开(公告)日：2017-06-13
申请号：US14069052
申请日：2013-10-31
Inventor： David J. Hammond , Julia T. Lathrop , Larisa Cervenakova , Ruben G. Carbonell
CPC classification number: G01N33/6896 , C07K5/0808 , C07K5/081 , C07K5/0812 , C07K5/0819 , C07K5/0821 , C07K14/47 , G01N2800/2828
Abstract: Ligands that bind to prion proteins and methods for using the ligands for detecting or removing a prion protein from a sample, such as a biological fluid or an environmental sample. The ligands are capable of binding to one or more forms of prion protein including cellular prion protein (PrPc), infectious prion protein (PrPsc), and recombinant prion protein (PrPr). Prions from various species, including humans and hamsters, are bound by the ligands. Also provided is a method of treating or retarding the development of a prion-associated pathology in a subject.
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