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公开(公告)号：US08481702B2
公开(公告)日：2013-07-09
申请号：US13763212
申请日：2013-02-08
申请人： Satoshi Inouye , Yuiko Sahara , Junichi Sato
发明人： Satoshi Inouye , Yuiko Sahara , Junichi Sato
CPC分类号： G01N33/581 , C07K14/43509 , C07K2319/00 , C07K2319/20 , C07K2319/60 , C12N9/0069 , C12Q1/66
摘要： The fusion protein comprising (1) a first region comprising the amino acid sequence of SEQ ID NO: 18 and (2) a second region comprising an amino acid sequence for a polypeptide containing at least one cysteine residue for binding to other useful compound via the thiol group can be modified by chemical modification, and thus has a high catalytic ability for a luminescence activity and is highly available for general purposes.
摘要翻译： 该融合蛋白包含（1）包含SEQ ID NO：18的氨基酸序列的第一区域和（2）第二区域，其包含含有至少一个半胱氨酸残基的多肽的氨基酸序列，用于通过 硫醇基可以通过化学改性进行改性，因此具有高的发光活性的催化能力，并且高度可用于一般目的。
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2.发明申请CODON-OPTIMIZED NUCLEIC ACID FOR CODING APO-CLYTIN-II AND METHOD FOR USING THE SAME 有权用于编码APO-CLYTIN-II的CODON优化核酸及其使用方法公开(公告)号：US20100273202A1
公开(公告)日：2010-10-28
申请号：US12694549
申请日：2010-01-27
申请人： Satoshi Inouye , Yuiko Sahara , Junichi Sato
发明人： Satoshi Inouye , Yuiko Sahara , Junichi Sato
CPC分类号： C12P21/02 , C07K14/43595 , C12N15/67 , C12Q1/025 , G01N2333/43595
摘要： A codon-optimization of nucleic acid for coding apo-clytin-II protein is provided. Luminescent activity of clytin-II is remarkably enhanced. Accordingly, compared with the conventional use of the wild-type clytin-II, an intracellular calcium ion can be detected much more easily.
摘要翻译： 提供了用于编码载脂蛋白-II蛋白的核酸的密码子优化。 clytin-II的发光活性明显增强。 因此，与野生型clytin-II的常规用途相比，可以更容易地检测到细胞内钙离子。
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公开(公告)号：US08569003B2
公开(公告)日：2013-10-29
申请号：US13908686
申请日：2013-06-03
申请人： Satoshi Inouye , Yuiko Sahara , Junichi Sato
发明人： Satoshi Inouye , Yuiko Sahara , Junichi Sato
IPC分类号： C12Q1/66
CPC分类号： G01N33/581 , C07K14/43509 , C07K2319/00 , C07K2319/20 , C07K2319/60 , C12N9/0069 , C12Q1/66
摘要： The fusion protein comprising (1) a first region comprising the amino acid sequence of SEQ ID NO: 18 and (2) a second region comprising an amino acid sequence for a polypeptide containing at least one cysteine residue for binding to other useful compound via the thiol group can be modified by chemical modification, and thus has a high catalytic ability for a luminescence activity and is highly available for general purposes.
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4.发明授权Codon-optimized nucleic acid for coding apo-clytin-II and method for using the same 有权密码子优化的用于编码载脂蛋白-II的核酸及其使用方法公开(公告)号：US08394603B2
公开(公告)日：2013-03-12
申请号：US12694549
申请日：2010-01-27
申请人： Satoshi Inouye , Yuiko Sahara , Junichi Sato
发明人： Satoshi Inouye , Yuiko Sahara , Junichi Sato
IPC分类号： C12Q1/02 , C07H21/04 , C07K14/435
CPC分类号： C12P21/02 , C07K14/43595 , C12N15/67 , C12Q1/025 , G01N2333/43595
摘要： A codon-optimization of nucleic acid for coding apo-clytin-II protein is provided. Luminescent activity of clytin-II is remarkably enhanced. Accordingly, compared with the conventional use of the wild-type clytin-II, an intracellular calcium ion can be detected much more easily.
摘要翻译： 提供了用于编码载脂蛋白-II蛋白的核酸的密码子优化。 clytin-II的发光活性明显增强。 因此，与野生型clytin-II的常规用途相比，可以更容易地检测到细胞内钙离子。
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5.发明申请Fusion protein of Fc-binding domain and calcium-binding photoprotein, gene encoding the same and use thereof 有权融合蛋白的Fc结合结构域和钙结合光蛋白，基因编码相同并使用公开(公告)号：US20080193980A1
公开(公告)日：2008-08-14
申请号：US11892294
申请日：2007-08-21
申请人： Satoshi Inouye , Yuiko Sahara , Junichi Sato
发明人： Satoshi Inouye , Yuiko Sahara , Junichi Sato
CPC分类号： C12N15/62 , C07K2319/20 , G01N33/84
摘要： The invention provides a calcium-binding photoprotein which has a high ability to bind to IgG and is capable of more sensitively detecting IgG. A fusion protein of the present invention composed of a ZZ domain having the ability to bind to the Fc region of IgG and the calcium-binding photoprotein apoaequorin is used for the detection and assay of calcium ions and the detection of IgG.
摘要翻译： 本发明提供了具有高结合IgG能力并能够更敏感地检测IgG的钙结合光蛋白。 本发明的融合蛋白由具有与IgG的Fc区结合的ZZ结构域和钙结合光蛋白脱辅基融合蛋白的融合蛋白用于钙离子的检测和测定以及IgG的检测。
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公开(公告)号：US08399616B2
公开(公告)日：2013-03-19
申请号：US12975710
申请日：2010-12-22
申请人： Satoshi Inouye , Yuiko Sahara , Junichi Sato
发明人： Satoshi Inouye , Yuiko Sahara , Junichi Sato
IPC分类号： C07K1/00
CPC分类号： G01N33/581 , C07K14/43509 , C07K2319/00 , C07K2319/20 , C07K2319/60 , C12N9/0069 , C12Q1/66
摘要： The fusion protein comprising (1) a first region comprising the amino acid sequence of SEQ ID NO: 18 and (2) a second region comprising an amino acid sequence for a polypeptide containing at least one cysteine residue for binding to other useful compound via the thiol group can be modified by chemical modification, and thus has a high catalytic ability for a luminescence activity and is highly available for general purposes.
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7.发明授权Fusion protein of Fc-binding domain and calcium-binding photoprotein, gene encoding the same and use thereof 有权融合蛋白的Fc结合结构域和钙结合光蛋白，基因编码相同并使用公开(公告)号：US07888087B2
公开(公告)日：2011-02-15
申请号：US11892294
申请日：2007-08-21
申请人： Satoshi Inouye , Yuiko Sahara , Junichi Sato
发明人： Satoshi Inouye , Yuiko Sahara , Junichi Sato
IPC分类号： C12N9/00 , C12N9/02 , C12N1/20 , C12N15/00 , C12Q1/00 , C12Q1/68 , C12P21/04 , C07H21/04 , C07K1/00
CPC分类号： C12N15/62 , C07K2319/20 , G01N33/84
摘要： The invention provides a calcium-binding photoprotein which has a high ability to bind to IgG and is capable of more sensitively detecting IgG. A fusion protein of the present invention composed of a ZZ domain having the ability to bind to the Fc region of IgG and the calcium-binding photoprotein apoaequorin is used for the detection and assay of calcium ions and the detection of IgG.
摘要翻译： 本发明提供了具有高结合IgG能力并能够更敏感地检测IgG的钙结合光蛋白。 本发明的融合蛋白由具有与IgG的Fc区结合的ZZ结构域和钙结合光蛋白脱辅基融合蛋白的融合蛋白用于钙离子的检测和测定以及IgG的检测。
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公开(公告)号：US08642281B2
公开(公告)日：2014-02-04
申请号：US13080385
申请日：2011-04-05
申请人： Satoshi Inouye , Yuiko Sahara , Takamitsu Hosoya
发明人： Satoshi Inouye , Yuiko Sahara , Takamitsu Hosoya
IPC分类号： G01N33/53 , G01N33/20 , G01N21/64 , C07D487/04 , C07D241/10 , C07K14/00
CPC分类号： G01N33/573 , C07D241/12 , C07D241/20 , C07D409/04 , C07D409/14 , C07D487/04 , C07D495/04 , C07D495/14 , C12N9/96 , C12Q1/66 , C12Q1/68 , G01N21/6428 , G01N33/53 , G01N2333/43595 , G01N2333/90241
摘要： Coelenterazine analogues with different luminescence properties from conventional ones and coelenteramide analogues with different fluorescence properties from conventional ones have been desired. The invention provides coelenterazine analogues modified at the 8-position of coelenterazine and coelenteramide analogues modified at the 2- or 3-position of coelenteramide.
摘要翻译： 已经期望具有不同于常规荧光发光性质的Coelenterazine类似物和具有不同于常规荧光性质的coelenteramide类似物。 本发明提供在coelenterazine和coelenteramide类似物的8位修饰的coelenterazine类似物，其在coelenteramide的2-或3-位修饰。
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公开(公告)号：US20090011460A1
公开(公告)日：2009-01-08
申请号：US11826915
申请日：2007-07-19
申请人： Satoshi Inouye , Yuiko Sahara
发明人： Satoshi Inouye , Yuiko Sahara
CPC分类号： C07K14/43595
摘要： A protein according to the invention can be used to detect or measure calcium ions is provided. Further the protein is useful as a reporter protein or a luminescence marker. A polynucleotide according to the invention is also useful as a reporter gene.
摘要翻译： 根据本发明的蛋白质可用于检测或测量提供的钙离子。 此外，蛋白质可用作报告蛋白或发光标记。 根据本发明的多核苷酸也可用作报道基因。
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公开(公告)号：US20080076156A1
公开(公告)日：2008-03-27
申请号：US11902030
申请日：2007-09-18
申请人： Satoshi Inouye , Yuiko Sahara
发明人： Satoshi Inouye , Yuiko Sahara
CPC分类号： C07K14/31 , C07K2319/61 , C07K2319/705 , C12N9/0069 , C12N15/62 , C12Y113/12005 , C12Y113/12007 , C12Y113/12013
摘要： A target protein is prepared as soluble protein using a recombinant protein expression system. An expression vector is used that includes (1) an expression-inducible promoter sequence; (2) a first coding sequence including a polynucleotide coding for a polypeptide that is represented by the formula (Z)n; and (3) a second coding sequence that includes a polynucleotide that codes for a target protein. A method of producing the target protein is also used that includes expressing protein using this expression vector.
摘要翻译： 使用重组蛋白表达系统制备靶蛋白作为可溶性蛋白质。 使用表达载体，其包括（1）表达诱导型启动子序列; （2）包含编码由式（Z）表示的多肽的多核苷酸的第一编码序列。 和（3）包含编码靶蛋白的多核苷酸的第二编码序列。 还使用包含使用该表达载体表达蛋白质的靶蛋白质的制造方法。
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