Fusion protein having luminescence activity
    1.
    发明授权
    Fusion protein having luminescence activity 有权
    具有发光活性的融合蛋白

    公开(公告)号:US08481702B2

    公开(公告)日:2013-07-09

    申请号:US13763212

    申请日:2013-02-08

    IPC分类号: C07H21/02 C12N15/00 C12N1/20

    摘要: The fusion protein comprising (1) a first region comprising the amino acid sequence of SEQ ID NO: 18 and (2) a second region comprising an amino acid sequence for a polypeptide containing at least one cysteine residue for binding to other useful compound via the thiol group can be modified by chemical modification, and thus has a high catalytic ability for a luminescence activity and is highly available for general purposes.

    摘要翻译: 该融合蛋白包含(1)包含SEQ ID NO:18的氨基酸序列的第一区域和(2)第二区域,其包含含有至少一个半胱氨酸残基的多肽的氨基酸序列,用于通过 硫醇基可以通过化学改性进行改性,因此具有高的发光活性的催化能力,并且高度可用于一般目的。

    Process for production of recombinant proteins as a soluble form
    10.
    发明申请
    Process for production of recombinant proteins as a soluble form 有权
    以可溶形式生产重组蛋白的方法

    公开(公告)号:US20080076156A1

    公开(公告)日:2008-03-27

    申请号:US11902030

    申请日:2007-09-18

    摘要: A target protein is prepared as soluble protein using a recombinant protein expression system. An expression vector is used that includes (1) an expression-inducible promoter sequence; (2) a first coding sequence including a polynucleotide coding for a polypeptide that is represented by the formula (Z)n; and (3) a second coding sequence that includes a polynucleotide that codes for a target protein. A method of producing the target protein is also used that includes expressing protein using this expression vector.

    摘要翻译: 使用重组蛋白表达系统制备靶蛋白作为可溶性蛋白质。 使用表达载体,其包括(1)表达诱导型启动子序列; (2)包含编码由式(Z)表示的多肽的多核苷酸的第一编码序列。 和(3)包含编码靶蛋白的多核苷酸的第二编码序列。 还使用包含使用该表达载体表达蛋白质的靶蛋白质的制造方法。