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    METHOD AND INSERT FOR DENSITY GRADIENT SEPARATION
    3.
    发明申请
    METHOD AND INSERT FOR DENSITY GRADIENT SEPARATION 有权
    密度梯度分离的方法和插值

    公开(公告)号：US20140087360A1

    公开(公告)日：2014-03-27

    申请号：US14115446

    申请日：2012-05-03

    Applicant: Stemcell Technologies Inc.

    Inventor： Steven M. Woodside

    IPC: G01N33/49 ,  B04B7/12 ,  G01N33/569 ,  C12M1/26

    CPC classification number: G01N33/491 ,  B01L3/5021 ,  B01L2200/0684 ,  B01L2300/0618 ,  B03D3/00 ,  B04B7/12 ,  C12M33/10 ,  G01N33/56972

    Abstract: An insert for a centrifuge tube suitable for use in density gradient separation is described. The insert includes a member sized to fit within the tube for dividing the tube into a top portion and a bottom portion. Optionally the insert has a support extending or depending from the member for positioning the member within the tube. At least two openings are located on the member so that a first opening is closer to a bottom end of the tube relative to a second opening when the insert is positioned in the centrifuge tube. Also described are methods for separating a target population of cells from a sample using the insert for a centrifuge tube.

    Abstract translation: 描述适用于密度梯度分离的离心管的插入物。 插入件包括尺寸适合于管内以将管分成顶部和底部的构件。 可选地，插入件具有从构件延伸或悬垂的支撑件，用于将构件定位在管内。 至少两个开口位于构件上，使得当插入件位于离心管中时，第一开口相对于第二开口更靠近管的底端。 还描述了使用离心管的插入物从样品中分离靶细胞群的方法。

    Method for separating cells
    4.
    发明授权
    Method for separating cells 有权
    分离细胞的方法

    公开(公告)号：US07316932B2

    公开(公告)日：2008-01-08

    申请号：US10260560

    申请日：2002-10-01

    Applicant: Steven M. Woodside

    Inventor： Steven M. Woodside

    IPC: G01N1/18

    CPC classification number: G01N35/00 ,  Y10S435/803 ,  Y10S436/824 ,  Y10T436/25 ,  Y10T436/25375

    Abstract: The present invention is related to a method for separating a first population of cells from a second population of cells in a sample by discontinuous density gradient separation using dense particles to target the first population of cells and a density separation medium (DSM) that is at least about 0.001 g/cm3 higher than the density of the second population of cells.

    Abstract translation: 本发明涉及一种通过使用致密颗粒以第一细胞群为目标的不连续密度梯度分离和密度分离介质（DSM）分离样品中的第一细胞群的第一细胞群的方法， 比第二细胞群体的密度高约0.001g / cm 3。

    Meniscus reducing member
    6.
    发明授权
    Meniscus reducing member 有权

    公开(公告)号：US10537891B2

    公开(公告)日：2020-01-21

    申请号：US15918798

    申请日：2018-03-12

    Applicant: STEMCELL TECHNOLOGIES INC

    Inventor： Oliver Egeler ,  Steven Woodside

    IPC: B01L3/00

    Abstract: A meniscus reducing member for use in a vessel for containing a liquid may include a physical surface feature overlying at least a portion of an interior surface of the vessel. The physical surface feature may have first and second inner surfaces that are generally parallel and at least a third surface extending between the first and second surfaces. The first inner surface, second inner surface and third surfaces may be configured to physically alter a receding contact angle between the liquid and the physical surface feature. A coating material may be applied to at least one of the surfaces of the physical surface feature to chemically alter the receding contact angle between the liquid and the coated surface whereby the receding contact angle formed between the liquid and the meniscus reducing member is between about 90 degrees and less than 180 degrees.

    Compositions and methods for obtaining enriched mesenchymal stem cell cultures
    7.
    发明授权
    Compositions and methods for obtaining enriched mesenchymal stem cell cultures 有权

    公开(公告)号：US10017741B2

    公开(公告)日：2018-07-10

    申请号：US14776866

    申请日：2014-03-12

    Applicant: StemCell Technologies Inc.

    Inventor： Brenton John Short ,  Christopher Duronio

    IPC: C12N5/0775

    Abstract: The disclosure provides a method of culturing cells of the mesenchymal cell lineage, said method comprising contacting the cells with a culture media comprising a CSF1R kinase inhibitor. The disclosure also provides a method of culturing cells from bone marrow and/or compact bone to enrich the cells with cells of the mesenchymal cell lineage comprising contacting the cells with a culture media comprising a CSF1R kinase inhibitor. Cell culture media comprising a CSF1R kinase inhibitor and useful for culturing cells of the mesenchymal cell lineage and/or enriching cells of the mesenchymal cell lineage is also provided.

    METHOD OF DIFFERENTIATING STEM CELLS
    8.
    发明申请
    METHOD OF DIFFERENTIATING STEM CELLS 有权
    差异化干细胞的方法

    公开(公告)号：US20130224857A1

    公开(公告)日：2013-08-29

    申请号：US13829783

    申请日：2013-03-14

    Applicant: STEMCELL TECHNOLOGIES INC.

    Inventor： Alexandra A. Blak ,  Sharon A. Louis ,  Michael John Riedel

    IPC: C12N5/071

    CPC classification number: C12N5/0608 ,  C12N5/0606 ,  C12N5/0623 ,  C12N2500/60 ,  C12N2500/90 ,  C12N2500/92 ,  C12N2500/98 ,  C12N2502/13 ,  C12N2506/02 ,  C12N2509/00 ,  C12N2533/32 ,  C12N2533/90

    Abstract: The present disclosure provides methods of generating germ layers from stem cells comprising culturing the stem cells in a culture media having osmolality ranges that promote the generation of specific germ layer progenitor cells. The present disclosure also includes a method to generate different cell lineages from the germ layers as well as to detect them by immunological methods. The present disclosure further provides methods for the generation, isolation, cultivation and propagation of committed progenitor cells and for the production of differentiated cells from the three germ layers. The present disclosure also provides culture media and kits for use in inducing the three germ layers.

    Abstract translation: 本公开提供了从干细胞产生胚层的方法，包括在培养基中培养干细胞，所述培养基具有促进特异性胚层祖细胞产生的渗透压浓度范围。 本公开还包括从胚芽层产生不同细胞谱系以及通过免疫学方法检测它们的方法。 本公开进一步提供了用于产生，分离，培养和繁殖定型祖细胞以及从三个胚层生产分化细胞的方法。 本公开还提供用于诱导三个胚层的培养基和试剂盒。

    Automated pipette machine
    9.
    发明授权
    Automated pipette machine 有权
    自动移液器

    公开(公告)号：US07819029B2

    公开(公告)日：2010-10-26

    申请号：US12164154

    申请日：2008-06-30

    Applicant: Andrew Angus ,  Fred Davis ,  Adam Donath ,  Richard Grant ,  Dirk Kurpershoek

    Inventor： Andrew Angus ,  Fred Davis ,  Adam Donath ,  Richard Grant ,  Dirk Kurpershoek

    IPC: G01N1/14 ,  B01L3/02 ,  G01N35/10

    CPC classification number: B01L3/0279 ,  B01L2200/023 ,  B01L2200/0689 ,  G01N35/10

    Abstract: There is also provided an apparatus for use on an automated pipette machine for transmitting pressure changes produced by a pump on the machine to a pipette nozzle. The apparatus comprises a housing defining a chamber a first conduit and a second conduit. The first conduit extends into the chamber and has a first opening positioned in the chamber. The first conduit is fluidically connectible to the nozzle. The second conduit extends into the chamber and has a first opening positioned in the chamber. The second conduit is fluidically connectible to the pump. The first opening of the first conduit is positioned above the first opening of the second conduit. The chamber defines at least a selected volume between the height of the first opening of the first conduit and the height of the first opening of the second conduit.

    Abstract translation: 还提供了一种用于自动移液器的用于将由机器上的泵产生的压力变化传递到移液管喷嘴的装置。 该装置包括限定腔室的壳体，第一导管和第二导管。 第一导管延伸到腔室中并且具有位于腔室中的第一开口。 第一导管可流体地连接到喷嘴。 第二导管延伸到腔室中并且具有位于腔室中的第一开口。 第二导管可流体地连接到泵。 第一导管的第一开口位于第二导管的第一开口的上方。 腔室限定第一导管的第一开口的高度与第二导管的第一开口的高度之间的至少一个选定的体积。

    Method for the discrimination and isolation of mammary epithelial stem and colony-forming cells
    10.
    发明授权
    Method for the discrimination and isolation of mammary epithelial stem and colony-forming cells 有权
    用于鉴别和分离乳腺上皮干细胞和集落形成细胞的方法

    公开(公告)号：US07723047B2

    公开(公告)日：2010-05-25

    申请号：US11758964

    申请日：2007-06-06

    Applicant: John Stingl ,  Connie J. Eaves

    Inventor： John Stingl ,  Connie J. Eaves

    IPC: G01N33/53 ,  C12N5/08

    CPC classification number: C12N5/0631 ,  C07K16/2803 ,  C07K16/2842 ,  C07K16/289 ,  C07K16/2896 ,  C12N2501/599

    Abstract: The present invention relates to an improved method that permits the differential isolation of mouse mammary stem cells and colony forming cells (CFCs). The method involves depletion of non-epithelial cells from freshly dissociated mouse mammary tissue by incubation with an antibody composition containing antibodies specific for CD45, Ter119, CD35 and optionally CD140a. After formation of conjugates between the non-epithelial mammary cells and the antibodies specific for CD45, Ter119, CD35 and optionally CD140a, the cell conjugates are removed and the remaining epithelial cells are then incubated with an antibody composition containing antibodies specific for CD24 and CD49f or CD24 and CD14. After formation of conjugates between the epithelial cells and the antibodies specific for CD24 and CD49f or CD24 and CD14, the mouse mammary stem and the luminal-restricted CFC cells can be differentially isolated. The invention also relates to kits for carrying out this method and to the cell preparations prepared by this method.

    Abstract translation: 本发明涉及允许小鼠乳腺干细胞和集落形成细胞（CFC）的差异分离的改进方法。 该方法涉及通过与含有对CD45，Ter119，CD35和任选的CD140a特异性抗体的抗体组合物孵育而从新解离的小鼠乳腺组织中消耗非上皮细胞。 在形成非上皮乳腺细胞和对CD45，Ter119，CD35和任选的CD140a特异性抗体之间的缀合物之后，移除细胞缀合物，然后将剩余的上皮细胞与含有对CD24和CD49f特异性抗体的抗体组合物孵育，或 CD24和CD14。 在形成上皮细胞和CD24和CD49f或CD24和CD14特异性抗体之间的缀合物之后，可以差异分离小鼠乳腺干和腔限制的CFC细胞。 本发明还涉及用于实施该方法的试剂盒以及通过该方法制备的细胞制剂。

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