公开(公告)号：US20240329063A1

公开(公告)日：2024-10-03

申请号：US18579109

申请日：2022-07-14

申请人： Centro Cardiologico Monzino S.P.A. ,  Universita' Degli Studi di Milano

发明人： Marina Camera ,  Marta Brambilla ,  Paola Canzano

IPC分类号： G01N33/86

CPC分类号： G01N33/86 ,  G01N2333/7454 ,  G01N2440/14

摘要： Disclosed is a method for evaluating residual platelet thrombotic potential in patients undergoing antiplatelet treatment, which comprises determination in a patient's blood sample of vasodilator-stimulated phosphoprotein phosphorylation (VASP-P) status and platelet tissue factor (TF) expression.

公开(公告)号：US20080026447A1

公开(公告)日：2008-01-31

申请号：US11788350

申请日：2007-04-19

申请人： Yuval Blat

发明人： Yuval Blat

IPC分类号： G01N33/48 ,  C12N9/50 ,  G01N33/566

CPC分类号： C12Q1/56 ,  G01N33/86 ,  G01N2333/7454 ,  G01N2333/96444 ,  G01N2333/96447

摘要： This invention provides a method for determining the concentration of a factor VIIa inhibitor in a sample. A method for determining non specific binding of a factor VIIa inhibitor to proteins other than factor VIIa is also provided.

摘要翻译： 本发明提供了一种测定样品中因子VIIa抑制剂浓度的方法。 还提供了确定因子VIIa抑制剂与因子VIIa以外的蛋白质的非特异性结合的方法。

公开(公告)号：US20060034846A1

公开(公告)日：2006-02-16

申请号：US10822943

申请日：2004-04-12

申请人： Mirella Ezban ,  Nathalie Busso

发明人： Mirella Ezban ,  Nathalie Busso

IPC分类号： A61K39/395 ,  A61K38/37

CPC分类号： G01N33/564 ,  A61K38/4846 ,  C07K16/2896 ,  G01N2333/7454 ,  G01N2500/00 ,  G01N2800/102 ,  G01N2800/105 ,  Y02A50/401

摘要： Methods of inducing, promoting, and/or enhancing one or more physiological responses associated with the treatment and/or prevention of inflammatory arthritides and/or other inflammation-related conditions in mammals and/or mammalian tissues including delivery of an effective amount of at least one tissue factor antagonist, at least one tissue factor inhibitor, or a combination thereof to a mammal and/or mammalian tissue under conditions such that such a physiological response is induced, promoted, and/or enhanced.

摘要翻译： 在哺乳动物和/或哺乳动物组织中诱导，促进和/或增强与治疗和/或预防炎性关节炎和/或其他炎症相关病症相关的一种或多种生理反应的方法，包括递送有效量的至少 一种组织因子拮抗剂，至少一种组织因子抑制剂或其组合，在诱导，促进和/或增强这种生理反应的条件下与哺乳动物和/或哺乳动物组织接触。

公开(公告)号：US20040086953A1

公开(公告)日：2004-05-06

申请号：US10288249

申请日：2002-11-05

发明人： Richard Jenny ,  Paul E. Haley ,  Brian Earp

IPC分类号： C12Q001/56

CPC分类号： C12Q1/56 ,  G01N2333/7454

摘要： A method for manufacturing a tissue factor-based prothrombin-time reagent includes combining predetermined quantities of tissue factor (TF), phospholipid and detergent-containing buffer to create a TF/phospholipid mixture. In this combining step, the quantity of TF is predetermined based upon its measured TF activity. Next, the detergent is removed from the TF/phospholipid mixture to produce an essentially detergent-free TF/phospholipid mixture that is useful as a tissue factor-based PT reagent. The method can include the use of hydrophobic resin that has been prescreened based on phospholipid recovery to accomplish the removal of the detergent.

摘要翻译： 用于制造基于组织因子的凝血酶原时间试剂的方法包括组合预定量的组织因子（TF），磷脂和含洗涤剂的缓冲液以产生TF /磷脂混合物。 在该组合步骤中，TF的量基于其测量的TF活性而预先确定。 接下来，将去污剂从TF /磷脂混合物中除去以产生可用作基于组织因子的PT试剂的基本上不含洗涤剂的TF /磷脂混合物。 该方法可以包括使用已经基于磷脂回收预筛选以完成去除洗涤剂的疏水性树脂。

公开(公告)号：US20020110486A1

公开(公告)日：2002-08-15

申请号：US10121636

申请日：2002-04-11

发明人： Robert Justice Shartle ,  Herbert Chow ,  Christa Hartmann

IPC分类号： G01N031/22

CPC分类号： B01L3/5027 ,  B01L3/502723 ,  B01L3/502738 ,  B01L2200/0621 ,  B01L2200/12 ,  B01L2300/0681 ,  B01L2300/0822 ,  B01L2300/0825 ,  B01L2300/0864 ,  B01L2300/087 ,  B01L2300/0887 ,  B01L2400/0406 ,  B01L2400/0481 ,  B01L2400/0688 ,  G01N21/8483 ,  G01N33/4905 ,  G01N33/525 ,  G01N33/5304 ,  G01N33/54386 ,  G01N33/558 ,  G01N2333/7454 ,  G01N2333/96447 ,  Y10T436/112499 ,  Y10T436/25375

摘要： A fluidic medical diagnostic device permits measurement of analyte concentration or a property of a biological fluid, particularly the coagulation time of blood. The device has at one-end a sample port for introducing a sample and at the other end a bladder for drawing the sample to a measurement area. A channel carries the sample from the sample port to the measurement area, and a stop junction, between the measurement area and bladder, halts the sample flow. The desired measurement can be made by placing the device into a meter which measures a physical property of the samplenulltypically, optical transmittancenullafter it has interacted with a reagent in the measurement area.

摘要翻译： 流体医学诊断装置允许测量分析物浓度或生物流体的性质，特别是血液的凝固时间。 该装置在一端具有用于引入样品的样品端口，另一端具有用于将样品抽吸到测量区域的气囊。 通道将样品从样品端口运送到测量区域，测量区域和气囊之间的停止点停止样品流动。 可以通过将装置放置在测量样品的物理性质（通常为光学透射率）的仪表中，在与测量区域中的试剂相互作用之后进行所需的测量。

公开(公告)号：US20020098114A1

公开(公告)日：2002-07-25

申请号：US10061723

申请日：2002-02-01

发明人： Ian A. Harding ,  Robert Justice Shartle

IPC分类号： G01N033/52

CPC分类号： B05D3/00 ,  B01L3/5027 ,  B01L3/502707 ,  B01L2200/0621 ,  B01L2200/12 ,  B01L2200/16 ,  B01L2300/0681 ,  B01L2300/0822 ,  B01L2300/0825 ,  B01L2300/0864 ,  B01L2300/087 ,  B01L2300/0887 ,  B01L2400/0406 ,  B01L2400/0481 ,  B01L2400/0688 ,  G01N21/8483 ,  G01N33/4905 ,  G01N33/521 ,  G01N33/525 ,  G01N33/5304 ,  G01N33/54386 ,  G01N33/558 ,  G01N33/86 ,  G01N2333/7454 ,  G01N2333/96447

摘要： A medical diagnostic device has a non-absorbent substrate that has a hydrophilic target area on which a reagent is deposited by non-impact printing of microdroplets. During deposition, the device is moved relative to the stream of microdroplets to form a substantially uniform reagent layer on the substrate. The device is particularly well adapted for measuring blood coagulation times. In a preferred embodiment, coagulation times are determined by monitoring the optical transmission of light through the target area as an applied blood sample interacts with the reagent.

摘要翻译： 医疗诊断装置具有非吸收性基材，其具有亲水性目标区域，通过微滴的非冲击印刷沉积试剂。 在沉积期间，装置相对于微滴流移动，以在基底上形成基本均匀的试剂层。 该装置特别适合于测量凝血时间。 在优选的实施方案中，凝固时间是通过在施加的血液样品与试剂相互作用时监测通过目标区域的光的光学透射来确定的。

公开(公告)号：US5270451A

公开(公告)日：1993-12-14

申请号：US926134

申请日：1992-08-06

申请人： Pamela L. H. Hawkins ,  James R. Maynard

发明人： Pamela L. H. Hawkins ,  James R. Maynard

IPC分类号： A61K38/00 ,  C07K14/745 ,  C07K3/02 ,  C07K3/74 ,  C07K13/00

CPC分类号： C07K14/745 ,  A61K38/00 ,  G01N2333/7454

摘要： The Prothrombin Time (PT) is used as a screening test for blood coagulation factor deficiencies and for monitoring oral anti-coagulant therapy using coumadin. Thromboplastin reagents activate the extrinsic pathway of coagulation and are the basis for the Prothrombin Time (PT) test. This invention describes the use of barium sulfate and chaotropic agents, and nonionic detergents, for the extraction of sensitive thromboplastin reagents from tissue. Extraction with sodium thiocyanate alone also greatly enhances thromboplastin sensitivity. This invention should be useful for all thromboplastins and will improve their sensitivity for all PT-based tests and specific assays.

摘要翻译： 凝血酶原时间（PT）用作凝血因子缺乏症的筛选试验，并用于使用香豆素监测口服抗凝血剂治疗。 凝血酶原激活凝血因子的外在途径，是凝血酶原时间（PT）检测的基础。 本发明描述了硫酸钡和离液剂以及非离子型洗涤剂用于从组织中提取敏感的凝血激酶原试剂。 单独使用硫氰酸钠的提取也大大增强凝血活酶敏感性。 本发明对于所有的凝血活酶都应该是有用的，并且将提高它们对所有基于PT的试验和特异性测定的敏感性。

公开(公告)号：US20230314454A1

公开(公告)日：2023-10-05

申请号：US18329833

申请日：2023-06-06

申请人： Thrombo Translational Research Lab Inc.

发明人： Yuichi KAMIKUBO

IPC分类号： G01N33/86 ,  C07K14/745

CPC分类号： G01N33/86 ,  C07K14/745 ,  G01N2333/974 ,  G01N2333/96452 ,  G01N2333/7454

摘要： A highly sensitive blood coagulation test reagent that enables determining and evaluating bleeding risk of direct oral anticoagulants (DOACs) and the like accurately in hemorrhagic diseases, and the like are provided. A blood coagulation test reagent containing: an active form of a blood coagulation factor XI (FXIa); and tissue factor (TF). A blood coagulation test method including a step of measuring the amount of thrombin generated by reacting a biological sample as a test specimen with a test reagent containing: 0.5 pM to 1,000 pM of an active form of a blood coagulation factor XI(FXIa); and 1 fM to 1,000 fM of tissue factor.

公开(公告)号：US20180031539A1

公开(公告)日：2018-02-01

申请号：US15661313

申请日：2017-07-27

申请人： SYSMEX CORPORATION ,  PUBLIC UNIVERSITY CORPORATION NARA MEDICAL UNIVERSITY

发明人： Midori SHIMA ,  Keiji NOGAMI ,  Tomoko MATSUMOTO ,  Yuka TABUCHI ,  Nobuo ARAI

IPC分类号： G01N33/49 ,  G01N21/77 ,  G01N21/82 ,  G01N35/00

CPC分类号： G01N33/4905 ,  G01N21/77 ,  G01N21/82 ,  G01N33/86 ,  G01N35/00584 ,  G01N2021/7783 ,  G01N2035/0097 ,  G01N2333/7454 ,  G01N2333/755 ,  G01N2800/224 ,  G01N2800/56 ,  G01N2800/7033

摘要： Disclosed is a method for determining the severity of hemophilia, the method including the steps of: coagulating a blood specimen to acquire a coagulation waveform; acquiring an average change rate of a coagulation rate from the coagulation waveform; and determining the severity of hemophilia in the blood specimen based on the average change rate of the coagulation rate.

公开(公告)号：US20160091513A1

公开(公告)日：2016-03-31

申请号：US14891119

申请日：2014-02-21

申请人： STRUSZYM, S.L.

发明人： Bernat GALMÉS SUREDA ,  Mariana Isabel CANARO HIRNYK ,  Vicente R. CORTINA GINER

IPC分类号： G01N33/86

CPC分类号： G01N33/86 ,  B01L3/502 ,  B01L3/5027 ,  B01L2300/024 ,  B01L2300/0825 ,  B01L2300/0838 ,  B01L2300/0864 ,  C12Q1/56 ,  G01N2333/7454

摘要： Test strips for determining the activity of a coagulation factor in a blood sample are provided. The strip comprises a support, a sample inlet port for deposition of a blood sample, and a reaction area comprising a blood coagulation reagent. The sample inlet port is connected to the reaction area, and the coagulation reagent comprises blood plasma deficient in the coagulation factor for which activity is to be measured, an ionic citrate source an ionic calcium source, and either one or more coagulation contact phase activator reagents and phospholipids or a mixture of tissue factor and phospholipids. The disclosure further relates to in vitro methods for measuring an activity of a coagulation factor.

摘要翻译： 提供了用于测定血液样品中凝血因子活性的测试条。 该条带包括支撑体，用于沉积血液样本的样本入口端口和包括凝血试剂的反应区域。 样品入口连接到反应区域，凝血试剂包括血液中缺乏要测量活性的凝血因子，离子柠檬酸盐源，离子钙源和一种或多种凝血接触相活化剂试剂 和磷脂或组织因子和磷脂的混合物。 本公开还涉及用于测量凝血因子活性的体外方法。