-
公开(公告)号：US09885352B2
公开(公告)日：2018-02-06
申请号：US14867918
申请日：2015-09-28
发明人： Robert A. Yuan
CPC分类号： F04B53/10 , B01L3/502715 , B01L3/502738 , B01L2200/027 , B01L2200/0663 , B01L2300/0627 , B01L2300/0867 , B01L2400/0644 , F16K27/003 , G01N33/48721
摘要： A delivery system for a sensor chip includes a plurality of selectable ports arranged on a first assembly. Each of the selectable ports is in communication with a separate channel. The delivery system includes a second assembly movable in relation to the first assembly. The second assembly has a channel that is mechanically connectable to different ones of the plurality of selectable ports on the first assembly by motion of the second assembly relative to the first assembly. The delivery system includes a mechanical interface configured to engage a separate actuator so that relative motion of the first assembly and the second assembly is affected by the actuator.
-
公开(公告)号：US09869655B2
公开(公告)日：2018-01-16
申请号：US14558268
申请日：2014-12-02
发明人： Roger J. A. Chen
IPC分类号： G01N27/327 , G01N33/487
CPC分类号： G01N27/3278 , C12Q1/6874 , G01N27/44791 , G01N33/48721
摘要： A system for detecting electrical properties of a molecular complex is disclosed. The system includes an electrode electrically coupled to a molecular complex that outputs an electrical signal affected by an electrical property of the molecular complex, wherein the effect of the electrical property of the molecular complex on the electrical signal is characterized by an expected bandwidth. The system further includes an integrating amplifier circuit configured to: receive the electrical signal from the electrode. The integrating amplifier circuit is further configured to selectively amplify and integrate a portion of the electrical signal over time within a predetermined bandwidth, wherein the predetermined bandwidth is selected at least in part based on the expected bandwidth.
-
公开(公告)号：US09850534B2
公开(公告)日：2017-12-26
申请号：US14376836
申请日：2013-02-15
发明人： Randall Davis , Roger Chen
IPC分类号： C12Q1/68 , C12N15/11 , G01N33/487 , G01N33/543
CPC分类号： C12Q1/6869 , C12N15/111 , C12N2310/11 , C12N2310/16 , C12N2310/3517 , C12N2320/10 , C12N2320/32 , G01N33/48721 , G01N33/5432 , C12Q2565/607 , C12Q2565/631
摘要： The present disclosure provides biochips and methods for making biochips. A biochip can comprise a nanopore in a membrane (e.g., lipid bilayer) adjacent or in proximity to an electrode. Methods are described for forming the membrane and inserting the nanopore into the membrane. The biochips and methods can be used for nucleic acid (e.g., DNA) sequencing. The present disclosure also describes methods for detecting, sorting, and binning molecules (e.g., proteins) using biochips.
-
公开(公告)号：US09791432B2
公开(公告)日：2017-10-17
申请号：US14664484
申请日：2015-03-20
发明人： Robert A. Yuan
IPC分类号： G01N33/48 , G01N33/487 , C12Q1/68 , G01N27/447
CPC分类号： G01N33/48721 , C12Q1/6869 , G01N27/44717 , G01N27/44791
摘要： A nanopore based sequencing system is disclosed. The system includes a plurality of nanopore sensors, each nanopore sensor having a top portion for receiving a fluid. The system further includes an inlet delivering the fluid into the nanopore based sequencing system and an outlet delivering the fluid out of the nanopore based sequencing system. The system includes a fluid chamber that comprises one or more fluid flow channels above top portions of the nanopore sensors; wherein the fluid chamber includes at least one divider that limits the width of the one or more fluid flow channels. In some embodiments, the at least one divider limits the width of the one or more fluid flow channel based on whether the surface tension and adhesive forces between the fluid and the fluid flow channel surfaces are sufficient to prevent the fluid from collapsing within the fluid flow channel.
-
公开(公告)号：US20170284962A1
公开(公告)日：2017-10-05
申请号：US15487825
申请日：2017-04-14
IPC分类号： G01N27/447 , G01N27/403 , G01N33/487 , C12Q1/68
CPC分类号： G01N27/44791 , C12Q1/6869 , G01N27/403 , G01N33/48721 , C12Q2527/125 , C12Q2565/607 , C12Q2565/629 , C12Q2565/631
摘要： A nanopore based sequencing chip package is disclosed. The nanopore based sequencing chip package includes a reservoir defined by a plurality of surfaces. The chip package includes a nanopore cell array comprising a plurality of nanopore sensor cells enclosed by the reservoir. Each nanopore sensor cell has a working electrode. At least one surface of the reservoir is configured to be in contact with a conducting fluid when the conducting fluid is flowing through the reservoir. The chip package further includes a counter electrode disposed on the at least one surface of the reservoir.
-
公开(公告)号：US20170199151A1
公开(公告)日：2017-07-13
申请号：US15380721
申请日：2016-12-15
发明人： Roger J.A. Chen
IPC分类号： G01N27/447 , C12Q1/68 , G01N33/487
CPC分类号： G01N27/3278 , C12Q1/6874 , G01N27/44791 , G01N33/48721
摘要： A system for communicating information from an array of sensors is disclosed. The system comprises a sensor array that includes a plurality of sensors, wherein each sensor senses a physical property of a material that is in communication with the sensor. The system further comprises signal processing circuitry associated with each sensor that integrates the output of the sensor over time and compares the integrated output to a threshold. The system further comprises a communication network coupled to the signal processing circuitry that outputs information indicating that the integrated output corresponding to a given sensor has reached the threshold.
-
公开(公告)号：US20170153205A1
公开(公告)日：2017-06-01
申请号：US15288107
申请日：2016-10-07
发明人： Roger J.A. Chen
IPC分类号： G01N27/447 , G01N33/487
CPC分类号： G01N27/44791 , G01N27/3278 , G01N27/4473 , G01N27/44743 , G01N33/48721
摘要： A method of identifying a molecule is disclosed. A molecule is drawn to a nanopore by applying a first voltage signal to a pair of electrodes during a first period, wherein the first voltage signal causes a first ionic current through the nanopore that is indicative of a property of a portion of the molecule proximate to the nanopore. The molecule is released from the nanopore by applying a second voltage signal to the pair of electrodes during a second period, wherein the second voltage signal causes a second ionic current through the nanopore. The first period and the second period are determined based at least in part on a net ionic current through the nanopore comprising the first ionic current and the second ionic current.
-
公开(公告)号：US20170089858A1
公开(公告)日：2017-03-30
申请号：US14864400
申请日：2015-09-24
发明人： Santiago Fernandez-Gomez , Hui Tian , Bill Maney , Seung Shin
IPC分类号： G01N27/40 , G01N33/487
CPC分类号： G01N27/40 , G01N33/48721
摘要： A system includes a circuit configured to detect a voltage corresponding to an electrical measurement of a nanopore. The system also includes a component configured to compare the voltage to another voltage. Based at least in part on the comparison, a one bit indicator is determined. The one bit indicator indicates whether the voltage indicates a change in a state of the nanopore. In the event it is determined that the voltage indicates the change in the state of the nanopore, a multiple bit signal is provided for output.
-
公开(公告)号：US20170058397A1
公开(公告)日：2017-03-02
申请号：US14841120
申请日：2015-08-31
发明人： Morgan Mager , John Foster
CPC分类号： C23C14/35 , C12Q1/6874 , C23C14/0641 , C23C14/3414 , G01N33/48721
摘要： A nanopore cell is disclosed. The nanopore cell includes an electrolyte well having a bottom base, a surrounding sidewall, and a hydrophobic surface above the surrounding sidewall. The nanopore cell further includes a first layer of electrode material disposed on the bottom base of the electrolyte well. The nanopore cell further includes a second layer of electrode material disposed on the surrounding sidewall of the electrolyte well and electrically connected to the first layer of electrode material. The first layer of electrode material and the second layer of electrode material are configured to jointly provide capacitive coupling when an electrolyte is placed in the electrolyte well.
摘要翻译： 公开了一种纳米孔细胞。 纳米孔单元包括电解质阱，其具有底部基底，周围侧壁和在周围侧壁上方的疏水表面。 纳米孔电池还包括设置在电解质阱的底部基底上的第一电极材料层。 纳米孔单元还包括设置在电解质阱的周围侧壁上并电连接到第一电极材料层的第二电极材料层。 第一层电极材料和第二层电极材料构造成当电解质放置在电解质井中时共同提供电容耦合。
-
公开(公告)号：US09567630B2
公开(公告)日：2017-02-14
申请号：US14521425
申请日：2014-10-22
发明人： Randall W. Davis , Edward Shian Liu , Eric Takeshi Harada , Anne Aguirre , Andrew Trans , James Pollard , Cynthia Cech
IPC分类号： G01N27/327 , C12Q1/68 , C12M1/34 , G01N33/487
CPC分类号： C12Q1/6869 , C12M1/34 , G01N27/3277 , G01N27/3278 , G01N27/44791 , G01N33/48721 , C12Q2565/607 , C12Q2565/631
摘要： This disclosure provides a biochip comprising a plurality of wells. The biochip includes a membrane that is disposed in or adjacent to an individual well of the plurality of wells. The membrane comprises a nanopore, and the individual well comprises an electrode that detects a signal upon ionic flow through the pore in response to a species passing through or adjacent to the nanopore. The electrode can be a non-sacrificial electrode. A lipid bilayer can be formed over the plurality of wells using a bubble.
摘要翻译： 本公开提供了包括多个孔的生物芯片。 生物芯片包括设置在多个孔的单个井中或邻近多个井的单个井的膜。 膜包括纳米孔，并且单个孔包括电极，其响应于通过或邻近纳米孔的物质而在离子流动通过孔时检测信号。 电极可以是非牺牲电极。 可以使用气泡在多个孔上形成脂质双层。
-
-
-
-
-
-
-
-
-
搜索结果
153 条记录 (耗时 0.026 秒)
