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256 条记录 (耗时 0.061 秒)

    Method of producing saccharide preparations
    94.
    发明授权
    Method of producing saccharide preparations 失效
    生产糖制剂的方法

    公开(公告)号：US06303346B1

    公开(公告)日：2001-10-16

    申请号：US09632392

    申请日：2000-08-04

    申请人： Gin C. Liaw ,  Sven Pedersen ,  Hanne Vang Hendriksen ,  Allan Svendsen ,  Bjarne Rønfeldt Nielsen ,  Ruby Illum Nielsen

    发明人： Gin C. Liaw ,  Sven Pedersen ,  Hanne Vang Hendriksen ,  Allan Svendsen ,  Bjarne Rønfeldt Nielsen ,  Ruby Illum Nielsen

    IPC分类号： C12P1914

    CPC分类号： C12N9/2428 ,  C12P19/12 ,  C12P19/14 ,  C12P19/18 ,  C12P19/20

    摘要： The present invention relates to a method for the production of saccharide preparations, i.e., syrups, by saccharifying a liquefied starch solution, which method comprises a saccharification step during which step one or more enzymatic saccharification stages takes place, and the subsequent steps of one or more high temperature membrane separation steps, and re-circulation of the saccharification enzyme, in which method the membrane separation steps are carried out as an integral part of the saccharification step. In another specific aspect, the invention provides a method of producing a saccharide preparation, which method comprises an enzymatic saccharification step, and the subsequent steps of one or more high temperature membrane separation steps and re-circulation of the saccharification enzyme.

    摘要翻译： 本发明涉及通过糖化液化淀粉溶液来生产糖制剂（即糖浆）的方法，该方法包括糖化步骤，在步骤中进行一个或多个酶促糖化阶段，随后的步骤包括一个或多个 更高温的膜分离步骤和糖化酶的再循环，其中膜分离步骤作为糖化步骤的组成部分进行。 在另一个具体方面，本发明提供了一种制备糖制剂的方法，该方法包括酶糖化步骤，以及随后的一个或多个高温膜分离步骤和糖化酶再循环的步骤。

    Haloperoxidases with altered pH profiles
    95.
    发明授权
    Haloperoxidases with altered pH profiles 有权
    具有改变的pH曲线的过氧化氢酶

    公开(公告)号：US06221821B1

    公开(公告)日：2001-04-24

    申请号：US09271778

    申请日：1999-03-18

    申请人： Allan Svendsen ,  Louise Jørgensen

    发明人： Allan Svendsen ,  Louise Jørgensen

    IPC分类号： C12N908

    CPC分类号： C12N9/0065 ,  C09D5/14 ,  C11D3/38654

    摘要： Variants of a parent vanadium-containing haloperoxidase, which variant has haloperoxidase activity and an altered pH optimum and comprises a mutation in a position corresponding to at least one of the following positions: R490A, L, I, Q, M, E, D; A399G; F397N, Y, E, Q; P395A, S; R360A, L, I, Q, M, E, D; K353Q, M; S402A, T, V, S; D292L; A501S; W350F, Y; V495A, T, V, S; K394 A, L, I, Q, M, E, D; wherein the parent haloperoxidase has the amino acid sequence given in SEQ ID No. 1, or the parent haloperoxidase has an amino acid sequence which is at least 80% homologous to SEQ ID No. 1.

    摘要翻译： 母体含钒卤代过氧化物酶的变体，其变体具有卤代过氧化物酶活性和改变的pH最佳值，并且在与以下至少一个位置相对应的位置包含突变：R490A，L，I，Q，M，E，D; A399G; F397N，Y，E，Q; P395A，S; R360A，L，I，Q，M，E，D; K353Q，M; S402A，T，V，S; D292L; A501S; W350F，Y; V495A，T，V，S; K394 A，L，I，Q，M，E，D; 其中母体卤代过氧化物酶具有SEQ ID No.1所示的氨基酸序列，或者母体过氧化氢酶具有与SEQ ID No.1至少80％同源的氨基酸序列。

    Laccase mutants
    96.
    发明授权
    Laccase mutants 有权
    漆酶突变体

    公开(公告)号：US06218170B1

    公开(公告)日：2001-04-17

    申请号：US09518901

    申请日：2000-03-06

    申请人： Allan Svendsen

    发明人： Allan Svendsen

    IPC分类号： C12N120

    CPC分类号： C12Y110/03002 ,  C11D3/38654 ,  C11D3/38663 ,  C11D3/38672 ,  C12N9/0061

    摘要： The present invention relates to laccase mutants with increased oxidation potential and/or changed pH optimum and/or altered mediator pathway and/or altered O2/OH− pathway.

    摘要翻译： 本发明涉及具有增加的氧化电位和/或改变的pH最佳和/或改变的介体途径和/或改变的O 2 / OH途径的漆酶突变体。

    DNA sequences encoding insulin precursors and methods of production
    99.
    发明授权
    DNA sequences encoding insulin precursors and methods of production 失效
    编码胰岛素前体的DNA序列和生产方法

    公开(公告)号：US5324641A

    公开(公告)日：1994-06-28

    申请号：US952696

    申请日：1992-09-23

    申请人： Ib Jonassen ,  Ib G. Clausen ,  Ejner B. Jensen ,  Allan Svendsen

    发明人： Ib Jonassen ,  Ib G. Clausen ,  Ejner B. Jensen ,  Allan Svendsen

    IPC分类号： C12N15/09 ,  A61K38/00 ,  C07K14/575 ,  C07K14/62 ,  C12N15/17 ,  C12P21/02 ,  C12R1/865 ,  C12N15/00 ,  C12N1/08 ,  C12P21/06

    CPC分类号： C07K14/62 ,  A61K38/00

    摘要： DNA molecule and process for producing insulin precursors having the formula B(1-29) -H.sub.1 --X.sub.2 --Y.sub.2 --Y.sub.1 --A(1-21), wherein B(1-29) are the 29 first amino acid residues of the B chain of human insulin starting from the N-terminus, A(1-21) are the 21 amino acid residues of the A chain of human insulin, X.sub.1 represents a peptide bond or one naturally-occurring alpha-amino acid acid residues, X.sub.2 represents Glu or Asp, and Y.sub.1 and Y.sub.2 each represent Lys or Arg, the positions A6 and A11, A7 and B7 and A20 and B19, respectively, are connected through sulphur bridges, and, if desired, one or more of the glutamic acid residues in positions A4, A17, B13 and B21 are substituted by another naturally-occurring alpha-amino acid residue having an uncharged side chain, are provided. The insulin precursors are prepared by culturing a yeast strain transformed with a replicable plasmid comprising a DNA sequence encoding an insulin precursor of the above formula in a suitable medium and isolating the insulin precursor thus formed from the culture medium. The insulin precursor can be converted into human insulin or insulin analogues by enzymatic treatment in a manner known per se.

    摘要翻译： DNA分子和具有式B（1-29）-H1-X2-Y2-Y1-A（1-21）的胰岛素前体的制备方法，其中B（1-29）是B的第一个氨基酸残基 A（1-21）是人胰岛素A链的21个氨基酸残基，X1表示肽键或一个天然存在的α-氨基酸酸残基，X2表示 Glu或Asp，Y1和Y2各自表示Lys或Arg，位置A6和A11，A7和B7和A20和B19分别通过硫桥连接，如果需要，一个或多个谷氨酸残基在 位置A4，A17，B13和B21被另一个具有不带电侧链的天然存在的α-氨基酸残基所取代。 通过在合适的培养基中培养用包含编码上式胰岛素前体的DNA序列的可复制质粒转化的酵母菌株并分离由培养基形成的胰岛素前体来制备胰岛素前体。 胰岛素前体可以通过本身已知的方式通过酶处理转化为人胰岛素或胰岛素类似物。