-
公开(公告)号：US20110251073A1
公开(公告)日：2011-10-13
申请号：US12996538
申请日：2009-06-03
CPC分类号： C12N9/54 , A23K20/189 , C11D3/386 , C11D3/38681 , C11D11/0017 , C12N9/52 , C12Y304/21062
摘要： The present invention provides methods for protein engineering. Specifically, the invention provides methods utilizing site evaluation libraries to design libraries that optimize two or more properties of a protein. The present invention also provides variant subtilisins suitable for various uses.
摘要翻译： 本发明提供蛋白质工程方法。 具体地说，本发明提供了使用位点评估库来设计优化蛋白质的两个或多个性质的文库的方法。 本发明还提供适用于各种用途的变体枯草杆菌蛋白酶。
-
92.发明申请Systematic Evaluation of Sequence and Activity Relationships Using Site Evaluation Libraries For Engineering Multiple Properties 有权使用现场评估库对工程多重属性进行序列和活动关系的系统评估公开(公告)号：US20110082048A1
公开(公告)日：2011-04-07
申请号：US12778915
申请日：2010-05-12
申请人： David A. Estell , Wolfgang Aehle
发明人： David A. Estell , Wolfgang Aehle
CPC分类号： C12Q1/37 , C12N15/1086
摘要： The present invention provides methods for protein engineering. Specifically, the invention provides methods utilizing site evaluation libraries to design libraries that optimize two or more properties of a protein.
摘要翻译： 本发明提供蛋白质工程方法。 具体地说，本发明提供了使用位点评估库来设计优化蛋白质的两个或多个性质的文库的方法。
-
公开(公告)号：US07803902B2
公开(公告)日：2010-09-28
申请号：US12252062
申请日：2008-10-15
申请人： Neelam S. Amin , Katherine D. Collier , Melodie Estabrook , David A. Estell , Bryan P. Fox , Scott D. Power , Brian F. Schmidt , Gudrun Vogtentanz
发明人： Neelam S. Amin , Katherine D. Collier , Melodie Estabrook , David A. Estell , Bryan P. Fox , Scott D. Power , Brian F. Schmidt , Gudrun Vogtentanz
IPC分类号： C07K14/00
CPC分类号： C07K14/811 , A61K8/64 , A61Q7/02
摘要： The present invention relates to modified variant Bowman Birk Protease Inhibitor proteins (BBPIs) that comprise peptides that bind target proteins, and that are further modified to have greater protease inhibitory activity and/or be produced at greater yields than the unmodified BBPIs. The invention encompasses polynucleotide constructs and expression vectors containing polynucleotide sequences that encode the modified variant BBPIs, the transformed host cells that express and produce the modified variant BBPIs, the modified variant BBPI proteins, the compositions comprising the modified variant BBPIs, and the methods for making and using the modified variant BBPIs in personal care.
摘要翻译： 本发明涉及包含结合靶蛋白的肽的修饰变体Bowman Birk蛋白酶抑制剂蛋白（BBPI），并且被进一步修饰以具有比未修饰的BBPI更大的蛋白酶抑制活性和/或以更高的产量产生。 本发明包括多核苷酸构建体和含有编码修饰变体BBPI的多核苷酸序列的表达载体，表达和产生修饰变体BBPI的转化宿主细胞，修饰变体BBPI蛋白，包含修饰变体BBPI的组合物和制备方法 并在个人护理中使用修改后的变体BBPI。
-
94.发明申请PERSONAL CARE COMPOSITIONS COMPRISING MODIFIED VARIANT BOWMAN BIRK PROTEASE INHIBITORS 有权包含改性变体BAMMAN BIRK蛋白酶抑制剂的个人护理组合物公开(公告)号：US20100092409A1
公开(公告)日：2010-04-15
申请号：US12252032
申请日：2008-10-15
申请人： Neelam S. Amin , Katherine D. Collier , Melodie Estabrook , David A. Estell , Bryan P. Fox , Scott D. Power , Brian F. Schmidt , Gudrun Vogtentanz
发明人： Neelam S. Amin , Katherine D. Collier , Melodie Estabrook , David A. Estell , Bryan P. Fox , Scott D. Power , Brian F. Schmidt , Gudrun Vogtentanz
CPC分类号： A61K8/64 , A61K38/55 , A61Q1/02 , A61Q1/04 , A61Q1/06 , A61Q1/10 , A61Q3/00 , A61Q3/02 , A61Q5/006 , A61Q5/02 , A61Q5/06 , A61Q5/12 , A61Q17/04 , A61Q19/00 , A61Q19/002 , A61Q19/04 , A61Q19/10
摘要： The present invention relates to compositions that comprise modified variant Bowman Birk Protease Inhibitor proteins (BBPIs). The modified variant BBPIs comprise peptides that bind target proteins, and that are further modified to have greater protease inhibitory activity and/or be produced at greater yields than the unmodified BBPIs. The invention encompasses the compositions comprising the modified variant BBPIs, and the methods for making and using the compositions in personal care.
摘要翻译： 本发明涉及包含修饰的变体Bowman Birk蛋白酶抑制蛋白（BBPI）的组合物。 修饰的变体BBPI包含结合靶蛋白的肽，并且被进一步修饰以具有比未修饰的BBPI更大的蛋白酶抑制活性和/或以更高的产量产生。 本发明包括包含修饰变体BBPI的组合物，以及在个人护理中制备和使用组合物的方法。
-
公开(公告)号：US20090093393A1
公开(公告)日：2009-04-09
申请号：US12099632
申请日：2008-04-08
CPC分类号： G01N33/54393 , C07K7/06 , C07K7/08 , C12N15/1034 , C12N15/1037 , C12N15/1072 , C40B40/02 , G01N33/6845 , G01N2500/02
摘要： A selective targeting method is disclosed comprising contacting a library of ligands, particularly a peptide library, with an anti-target to allow the ligands to bind to the anti-target; separating the non-binding ligands from the anti-target bound ligands, contacting the non-binding anti-target ligands with a target allowing the unbound ligands to bind with the target to form a target-bound ligand complex; separating the target-bound ligand complex from ligands which do not bind to the target, and identifying the target-bound ligands on the target-bound ligand complex wherein the target-bound ligands have a KD in the range of about 10−7 to 10−10 M. Additionally claimed are the ligands identified according to the method.
摘要翻译： 公开了一种选择性靶向方法，其包括将配体文库，特别是肽文库与抗靶相接触，以允许配体结合抗靶; 将非结合配体与抗靶结合配体分离，使非结合抗靶配体与允许未结合配体与靶结合以形成靶结合配体复合物的靶; 将靶结合的配体络合物与不结合靶的配体分离，并鉴定靶结合的配体配合物上的靶结合配体，其中靶结合配体的KD在约10-7至10范围内 另外要求的是根据该方法鉴定的配体。
-
96.发明申请公开(公告)号：US20080234465A1
公开(公告)日：2008-09-25
申请号：US11848196
申请日：2007-08-30
申请人： Hans De Nobel , David A. Estell , Wei Liu , Scott D. Power , Brian Schmidt , Huaming Wang
发明人： Hans De Nobel , David A. Estell , Wei Liu , Scott D. Power , Brian Schmidt , Huaming Wang
CPC分类号： C12N15/80 , C07K14/811
摘要： Described herein are protease inhibitors, variants thereof and methods for their production.
摘要翻译： 本文描述的是蛋白酶抑制剂，其变体及其制备方法。
-
公开(公告)号：US20080233100A1
公开(公告)日：2008-09-25
申请号：US11972517
申请日：2008-01-10
申请人： Yiyou Chen , Anthony G. Day , David A. Estell , Christopher J. Murray , Scott D. Power , Volker Schellenberger
发明人： Yiyou Chen , Anthony G. Day , David A. Estell , Christopher J. Murray , Scott D. Power , Volker Schellenberger
CPC分类号： A61K38/46 , C07K2319/01 , C12N9/86 , C12Y305/02006
摘要： The present invention provides targeted enzymes that bind to targets better than the corresponding pre-targeted enzymes bind the target under like conditions, methods of making targeted enzymes, methods of using targeted enzymes to treat diseases, and pharmaceutical compositions comprising targeted enzymes.
摘要翻译： 本发明提供了靶向酶，其比相同的预靶向酶更好地结合靶，在相似条件下与目标酶结合，制备靶向酶的方法，使用靶向酶治疗疾病的方法，以及包含靶向酶的药物组合物。
-
公开(公告)号：US07329527B2
公开(公告)日：2008-02-12
申请号：US11014432
申请日：2004-12-16
申请人： David A. Estell
发明人： David A. Estell
摘要： The present invention relates to the identification of novel serine proteases in Gram-positive microorganisms. The present invention provides the nuclei acid and amino acid sequences for the Bacillus subtilis serine proteases SP1, SP2, SP3, SP4 and SP5. The present invention also provides host cells having a mutation or deletion of part or all of the gene encoding SP1, SP2, SP3, SP4 and SP5. The present invention also provides host cells further comprising nucleic acid encoding desired heterologous proteins such as enzymes. The present invention also provides a cleaning composition comprising a serine protease of the present invention.
-
公开(公告)号：US07329526B2
公开(公告)日：2008-02-12
申请号：US11014339
申请日：2004-12-16
申请人： David A. Estell
发明人： David A. Estell
摘要： The present invention relates to the identification of novel serine proteases in Gram-positive microorganisms. The present invention provides the nuclei acid and amino acid sequences for the Bacillus subtilis serine proteases SP1, SP2, SP3, SP4 and SP5. The present invention also provides host cells having a mutation or deletion of part or all of the gene encoding SP1, SP2, SP3, SP4 and SP5. The present invention also provides host cells further comprising nucleic acid encoding desired heterologous proteins such as enzymes. The present invention also provides a cleaning composition comprising a serine protease of the present invention.
摘要翻译： 本发明涉及革兰氏阳性微生物中新型丝氨酸蛋白酶的鉴定。 本发明提供枯草芽孢杆菌丝氨酸蛋白酶SP1，SP2，SP3，SP4和SP5的核酸和氨基酸序列。 本发明还提供具有编码SP1，SP2，SP3，SP4和SP5的基因的部分或全部的突变或缺失的宿主细胞。 本发明还提供宿主细胞，其进一步包含编码所需异源蛋白质如酶的核酸。 本发明还提供了包含本发明的丝氨酸蛋白酶的清洁组合物。
-
公开(公告)号：US07098021B2
公开(公告)日：2006-08-29
申请号：US11014361
申请日：2004-12-16
申请人： David A. Estell
发明人： David A. Estell
摘要： The present invention relates to the identification of a novel metalloproteases in gram positive microorganisms. The present invention provides the nucleic acid and amino acid sequences for the metalloprotease. The present invention also provides host cells having a mutation or deletion of part or all of the gene encoding the metalloprotease. The present invention provides host cells which further comprises a nucleic acid encoding desired heterologous proteins such as enzymes. The present invention also provides cleaning compositions, animal feeds and compositions used to treat a textile that include the metalloprotease of the present invention.
-
-
-
-
-
-
-
-
-
搜索结果
140 条记录 (耗时 0.097 秒)
