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公开(公告)号：US07825226B2
公开(公告)日：2010-11-02
申请号：US11978232
申请日：2007-10-26
申请人： Peter G. Schultz , Lei Wang , Zhiwen Zhang
发明人： Peter G. Schultz , Lei Wang , Zhiwen Zhang
CPC分类号： C12P21/005
摘要： Methods for making glycoproteins, both in vitro and in vivo, are provided. One method involves incorporating an unnatural amino acid into a protein and attaching one or more saccharide moieties to the unnatural amino acid. Another method involves incorporating an unnatural amino acid that includes a saccharide moiety into a protein. Proteins made by both methods can be further modified with additional sugars.
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92.发明授权公开(公告)号：US07824893B2
公开(公告)日：2010-11-02
申请号：US11663513
申请日：2005-09-20
CPC分类号： C07K14/195 , A61K47/64 , C07H21/04 , C12N9/93 , C12N15/67 , C12N15/70 , C12P21/00 , C12P21/02
摘要： The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetases that can incorporate alkynyl amino acids such as para-propargyloxyphenylalanine into proteins produced in a eubacteria host such as E. coli. The invention provides novel orthoghonal synthetases, methods for identifying and making the novel synthetases, methods for producing proteins containing alkynyl amino acids, and cellular translation systems.
摘要翻译： 本发明涉及tRNA和氨酰-tRNA合成酶的正交对，其可以将炔基氨基酸如对 - 炔丙氧基苯丙氨酸掺入到在诸如大肠杆菌的真细菌宿主中产生的蛋白质中。 本发明提供新颖的正交合成酶，用于鉴定和制备新型合成酶的方法，用于生产含有炔基氨基酸的蛋白质的方法和细胞翻译系统。
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公开(公告)号：US20100130476A1
公开(公告)日：2010-05-27
申请号：US12617630
申请日：2009-11-12
申请人： Weidong Wang , Peter G. Schultz
发明人： Weidong Wang , Peter G. Schultz
IPC分类号： A61K31/519 , C07D409/14 , C07D409/04 , C07D413/14 , C07D473/16 , C07D403/04 , C12N5/00 , A61K31/5377 , A61K31/52 , A61K31/551 , A61K31/517 , A61P3/10
CPC分类号： C07D409/04 , A61K31/4422 , A61K31/47 , A61K31/506 , A61K31/517 , A61K31/519 , A61K31/52 , C07D403/04 , C07D473/16
摘要： The present invention relates to compounds and compositions for inducing the expansion of pancreatic β-cells. The invention further relates to a use of these expanded pancreatic β-cells to reversibly expand pancreatic β-cells and other quiescent cells to overcome deficits associated with degenerative and/or autoimmune diseases.
摘要翻译： 本发明涉及用于诱导胰腺细胞扩增的化合物和组合物。 本发明还涉及这些扩展的胰腺细胞可逆地扩展胰腺细胞和其他静止细胞以克服与退行性和/或自身免疫疾病相关的缺陷的用途。
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94.发明授权Compositions of orthogonal lysyl-tRNA and aminoacyl-tRNA synthetase pairs and uses thereof 有权正交赖氨酰-tRNA和氨酰-tRNA合成酶对的组合及其用途公开(公告)号：US07638297B2
公开(公告)日：2009-12-29
申请号：US10563686
申请日：2004-07-07
摘要： Compositions and methods of producing components of protein biosynthetic machinery that include orthogonal lysyl-tRNAs, orthogonal lysyl-aminoacyl-tRNA synthetases, and orthogonal pairs of lysyl-tRNAs/synthetases, which incorporate homoglutamines into proteins are provided in response to a four base codon. Methods for identifying these orthogonal pairs are also provided along with methods of producing proteins with homoglutamines using these orthogonal pairs.
摘要翻译： 提供了蛋白质生物合成机理的组成和方法，其包括正交赖氨酰-tRNA，正交赖氨酰 - 酰基-tRNA合成酶和将高聚谷氨酰胺掺入蛋白质的正交对的赖氨酰-tRNA /合成酶，以响应于四碱基密码子。 还提供用于鉴定这些正交对的方法以及使用这些正交对产生具有高谷氨酰胺的蛋白质的方法。
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公开(公告)号：US07608423B2
公开(公告)日：2009-10-27
申请号：US11710672
申请日：2007-02-22
CPC分类号： C12N15/81 , A61K38/1709 , C12N9/93 , C12N15/67 , C12Y601/01 , C12Y601/01001 , C12Y601/0102 , Y10S435/81 , Y10S435/975
摘要： This invention provides compositions and methods for producing translational components that expand the number of genetically encoded amino acids in eukaryotic cells. The components include orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, orthogonal pairs of tRNAs/synthetases and unnatural amino acids. Proteins and methods of producing proteins with unnatural amino acids in eukaryotic cells are also provided.
摘要翻译： 本发明提供用于产生扩增真核细胞中遗传编码氨基酸数目的翻译组分的组合物和方法。 组分包括正交tRNA，正交氨酰-tRNA合成酶，正交对tRNA /合成酶和非天然氨基酸。 还提供了在真核细胞中产生具有非天然氨基酸的蛋白质的蛋白质和方法。
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公开(公告)号：US20090215117A1
公开(公告)日：2009-08-27
申请号：US12228146
申请日：2008-08-07
申请人： Peter G. Schultz , Lei Wang
发明人： Peter G. Schultz , Lei Wang
IPC分类号： C12P21/00
CPC分类号： C12N15/67 , C12N9/93 , C12N15/10 , C12P21/005
摘要： Compositions and methods of producing components of protein biosynthetic machinery that include orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of tRNAs/synthetases, which incorporate keto amino acids into proteins are provided. Methods for identifying these orthogonal pairs are also provided along with methods of producing proteins with keto amino acids using these orthogonal pairs.
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公开(公告)号：US20090163368A1
公开(公告)日：2009-06-25
申请号：US12290673
申请日：2008-10-31
申请人： Chang Liu , Meng-Lin Tsao , Vaughn Smider , Peter G. Schultz
发明人： Chang Liu , Meng-Lin Tsao , Vaughn Smider , Peter G. Schultz
CPC分类号： C12N15/1037 , C12N15/1058 , C12N15/67 , C40B40/08 , C40B40/10
摘要： The invention provides methods and compositions for screening polypeptide libraries that include variants comprising unnatural amino acids. In addition, the invention provides vector packaging systems and methods for packaging a nucleic acid in a vector. Compositions of vectors produced by the methods and systems are also provided
摘要翻译： 本发明提供了用于筛选多肽文库的方法和组合物，其包括包含非天然氨基酸的变体。 此外，本发明提供了用于在载体中包装核酸的载体包装系统和方法。 还提供了由方法和系统生成的载体的组合物
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98.发明申请公开(公告)号：US20080220472A1
公开(公告)日：2008-09-11
申请号：US11663513
申请日：2005-09-20
CPC分类号： C07K14/195 , A61K47/64 , C07H21/04 , C12N9/93 , C12N15/67 , C12N15/70 , C12P21/00 , C12P21/02
摘要： The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetases that can incorporate alkynyl amino acids such as para-propargyloxyphenylalanine into proteins produced in a eubacteria host such as E. coli. The invention provides novel orthoghonal synthetases, methods for identifying and making the novel synthetases, methods for producing proteins containing alkynyl amino acids, and cellular translation systems.
摘要翻译： 本发明涉及tRNA和氨酰-tRNA合成酶的正交对，其可以将炔基氨基酸如对 - 炔丙氧基苯丙氨酸掺入到在诸如大肠杆菌的真细菌宿主中产生的蛋白质中。 本发明提供新颖的正交合成酶，用于鉴定和制备新型合成酶的方法，用于生产含有炔基氨基酸的蛋白质的方法和细胞翻译系统。
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公开(公告)号：US20080176284A1
公开(公告)日：2008-07-24
申请号：US11978160
申请日：2007-10-26
申请人： Peter G. Schultz , Lei Wang
发明人： Peter G. Schultz , Lei Wang
IPC分类号： C12P21/02
CPC分类号： C12N15/67 , C12N9/93 , C12N15/10 , C12P21/005
摘要： Compositions and methods of producing components of protein biosynthetic machinery that include orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of tRNAs/synthetases, which incorporate keto amino acids into proteins are provided. Methods for identifying these orthogonal pairs are also provided along with methods of producing proteins with keto amino acids using these orthogonal pairs.
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公开(公告)号：US20080171317A1
公开(公告)日：2008-07-17
申请号：US11662814
申请日：2005-09-21
CPC分类号： C12P21/02 , C12N9/93 , G01N33/532 , G01N33/60 , G01N2458/15 , G01R33/1269 , Y10T436/24
摘要： Methods of producing and/or analyzing spectroscopically labeled proteins, e.g., proteins site-specifically labeled with NMR active isotopes, spin-labels, chelators for paramagnetic metals, and the like, are provided. The labeled proteins are produced in translation systems including orthogonal aminoacyl tRNA synthetase/tRNA pairs. Methods for assigning NMR resonances, e.g., methods using isotopically labeled proteins, are also provided.
摘要翻译： 提供了生产和/或分析光谱标记的蛋白质的方法，例如用NMR活性同位素特异性标记的蛋白质，自旋标记物，顺磁金属的螯合剂等。 标记的蛋白质在包括正交氨酰基tRNA合成酶/ tRNA对的翻译系统中产生。 也提供了用于分配NMR共振的方法，例如使用同位素标记的蛋白质的方法。
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