公开(公告)号：US08420893B2

公开(公告)日：2013-04-16

申请号：US12503482

申请日：2009-07-15

Applicant: William J. Gordon-Kamm ,  Timothy G. Helentjaris ,  Keith S. Lowe ,  Bo Shen ,  Mitchell C. Tarczynski ,  Peizhong Zheng

Inventor： William J. Gordon-Kamm ,  Timothy G. Helentjaris ,  Keith S. Lowe ,  Bo Shen ,  Mitchell C. Tarczynski ,  Peizhong Zheng

IPC: A01H5/00 ,  A01H1/00 ,  C12N15/29 ,  C12N15/82 ,  C12N5/04

CPC classification number: C12N15/8243 ,  A01H1/08 ,  C07K14/415 ,  C12N15/8201 ,  C12N15/8205 ,  C12N15/8209 ,  C12N15/8223 ,  C12N15/8231 ,  C12N15/8233 ,  C12N15/8245 ,  C12N15/8247 ,  C12N15/8261 ,  C12N15/8287 ,  Y02A40/146

Abstract: Methods and compositions for modulating plant development are provided. Nucleotide sequences and amino acid sequences encoding Ovule Development Protein 2 (ODP2) proteins are provided. The sequences can be used in a variety of methods including modulating development, developmental pathways, altering oil content in a plant, increasing transformation efficiencies, modulating stress tolerance, and modulating the regenerative capacity of a plant. Transformed plants, plant cells, tissues, and seed are also provided.

公开(公告)号：US08367913B2

公开(公告)日：2013-02-05

申请号：US12846320

申请日：2010-07-29

Applicant: Tim Uhrig ,  Paul Enger ,  Marie Michael ,  Ron Christensen

Inventor： Tim Uhrig ,  Paul Enger ,  Marie Michael ,  Ron Christensen

IPC: A01H5/00

CPC classification number: C12N15/8201

Abstract: Methods for transforming an explant are provided. The methods may include applying copper amino acid chelate to a plant and transforming an explant obtained therefrom. The transformed explant may have increased transformation frequency relative to a control.

Abstract translation: 提供了转化外植体的方法。 所述方法可包括将铜氨基酸螯合物施用于植物并转化由其获得的外植体。 转化的外植体相对于对照可能具有增加的转化频率。

公开(公告)号：US20120322116A1

公开(公告)日：2012-12-20

申请号：US13497894

申请日：2010-09-24

Applicant: Keishi Sakaguchi ,  Takanori Matsuda ,  Takumi Kobayashi ,  Makoto Ito ,  Naoki Nagano ,  Masahiro Hayashi ,  Daisuke Honda ,  Yosuke Taoka ,  Yuji Okita ,  Hitoshi Izumida ,  Shinichi Sugimoto

Inventor： Keishi Sakaguchi ,  Takanori Matsuda ,  Takumi Kobayashi ,  Makoto Ito ,  Naoki Nagano ,  Masahiro Hayashi ,  Daisuke Honda ,  Yosuke Taoka ,  Yuji Okita ,  Hitoshi Izumida ,  Shinichi Sugimoto

IPC: C12P7/64 ,  C12N15/79

CPC classification number: C12P7/6409 ,  C11C3/00 ,  C12N9/0071 ,  C12N9/0083 ,  C12N15/79 ,  C12N15/8201 ,  C12N15/8247 ,  C12P7/6427

Abstract: Disclosed is a transformation method whereby an ability to produce a useful substance of a stramenopile can be improved. The method for transforming a stramenopile comprises transferring a foreign gene into the stramenopile which is a microorganism belonging to the class Labyrinthula, more specifically, to a genus Labyrinthula, Altornia, Aplanochytrium, Schizochytrium, Aurantiochytrium, Thraustochytrium, Ulkenia, etc. Said foreign gene, which is a gene relating to tolerance against an antibiotic, a colorimetric protein and/or a fatty acid desaturase (Δ5 desaturase gene, Δ12 desaturase gene and/or ω3 desaturase gene), is transferred by using the electroporation or gene-gun technique.

Abstract translation: 公开了能够提高产生有机物的能力的转化方法。 用于转化stramenopile的方法包括将外源基因转移到作为属于Labyrinthula类的微生物的地幔中，更具体地是属于Labyrinthula，Altornia，Aplanochytrium，Schizochytrium，Aurantiochytrium，Thraustochytrium，Ulkenia等。所述外源基因， 其是与抗生素，比色蛋白质和/或脂肪酸去饱和酶（＆Dgr。5去饱和酶基因，＆Dgr; 12去饱和酶基因和/或ω3去饱和酶基因）的耐受相关的基因通过使用电穿孔或基因 - 枪技术。

公开(公告)号：US20120233724A1

公开(公告)日：2012-09-13

申请号：US13480275

申请日：2012-05-24

Applicant: John P. Davies ,  Hein Tsoeng (Medard) Ng ,  D. Ry Wagner

Inventor： John P. Davies ,  Hein Tsoeng (Medard) Ng ,  D. Ry Wagner

IPC: A01H5/00 ,  C12N15/82 ,  A23K1/00 ,  A23L1/212 ,  A23L1/36

CPC classification number: C12N15/8246 ,  C07K14/415 ,  C12N15/8201 ,  C12N15/8241 ,  C12N15/8251 ,  C12N15/8255 ,  C12N15/8261 ,  C12Q1/6895 ,  C12Q2600/13 ,  C12Q2600/156 ,  C12Q2600/16 ,  Y02A40/146

Abstract: The present invention is directed to plants that display an improved oil quantity phenotype or an improved meal quality phenotype due to altered expression of an IMQ nucleic acid. The invention is further directed to methods of generating plants with an improved oil quantity phenotype or improved meal quality phenotype.

Abstract translation: 本发明涉及由于IMQ核酸的表达改变而显示改善的油量表型或改善的膳食质量表型的植物。 本发明进一步涉及产生具有改善的油量表型或改良的膳食质量表型的植物的方法。

公开(公告)号：US20120180166A1

公开(公告)日：2012-07-12

申请号：US13355312

申请日：2012-01-20

Applicant: Anisha Akula ,  David R. Duncan ,  Brenda A. Lowe ,  Michael T. Mann ,  William L. Petersen ,  Jyoti R. Rout ,  David D. Songstad ,  Joel B. Wilks ,  Wanggen Zhang

Inventor： Anisha Akula ,  David R. Duncan ,  Brenda A. Lowe ,  Michael T. Mann ,  William L. Petersen ,  Jyoti R. Rout ,  David D. Songstad ,  Joel B. Wilks ,  Wanggen Zhang

IPC: C12N15/82 ,  C12N5/04 ,  B02C19/00 ,  C12N1/32

CPC classification number: C12N15/8205 ,  C12N15/8201 ,  C12N15/8202 ,  C12N15/8206 ,  C12N15/8207 ,  C12N15/8209 ,  C12N15/821 ,  C12N15/8265 ,  C12Q1/6876 ,  C12Q1/6895 ,  C12Q2600/13 ,  C12Q2600/158 ,  C12Q1/6827 ,  C12Q1/686

Abstract: A method of producing a stably transformed corn plant in a single container is demonstrated. This method allows for the automation of the transformation process and reduces labor, material, and ergonomic costs associated with traditional plant tissue culture systems.

Abstract translation: 证明了在单个容器中生产稳定转化的玉米植物的方法。 这种方法允许转换过程的自动化，并减少与传统植物组织培养系统相关的劳动力，材料和人体工学成本。

公开(公告)号：US08206983B2

公开(公告)日：2012-06-26

申请号：US13206637

申请日：2011-08-10

Applicant: P. Samuel Jayakumar ,  Jeffrey Beringer ,  Paul R. Schmitzer ,  Frank Burroughs ,  Robbi J. Garrison ,  William M. Ainley ,  Narasimha C. Samboju

Inventor： P. Samuel Jayakumar ,  Jeffrey Beringer ,  Paul R. Schmitzer ,  Frank Burroughs ,  Robbi J. Garrison ,  William M. Ainley ,  Narasimha C. Samboju

IPC: C12N5/00 ,  C12N5/02

CPC classification number: C12N5/04 ,  C12N5/0025 ,  C12N15/8201

Abstract: The subject invention provides simple and consistent methods to break suspension cell aggregates to single cells with intact primary cell walls. The subject invention relates in part to cell separation of suspension cell aggregates cultured in medium containing pectin-degrading enzymes or tubulin de-polymerizing compounds including colchicine. The subject invention also relates to novel uses of compounds for such purposes. Another aspect of the subject invention relates to transformation of the subject, isolated cells. Such processes simplify and integrate single-cell-based transformation and selection processes into transgenic and transplastomic event-generation work processes. The subject invention also removes technical constraints and produces marker-free and uniformly expressing transgenic lines in a high throughput fashion to support various needs of animal health, biopharma, and trait and crop protection platforms.

Abstract translation: 本发明提供简单且一致的方法来将悬浮细胞聚集体破碎成具有完整原始细胞壁的单个细胞。 本发明部分涉及在包含果胶降解酶或包含秋水仙素的微管蛋白去聚合化合物的培养基中培养的悬浮细胞聚集体的细胞分离。 本发明还涉及化合物用于这些目的的新用途。 本发明的另一方面涉及受试者，分离的细胞的转化。 这样的过程简化并将基于单细胞的转化和选择过程整合到转基因和转基因组事件生成工作过程中。 本发明还消除了技术限制，并以高通量方式产生无标记和均匀表达的转基因品系，以支持动物健康，生物制药和性状和作物保护平台的各种需要。

公开(公告)号：US20120096586A1

公开(公告)日：2012-04-19

申请号：US13335646

申请日：2011-12-22

Applicant: Joseph Michael PALYS

Inventor： Joseph Michael PALYS

IPC: A01H1/00 ,  C12Q1/02 ,  G01N33/53 ,  C12Q1/68

CPC classification number: C12Q1/6895 ,  C12N15/8201 ,  C12N15/8205 ,  C12N15/8209 ,  C12N15/821 ,  C12N15/8265 ,  C12N15/8286 ,  Y02A40/162

Abstract: Methods for identification of successful transformation without a need for in vitro selection are provided. Direct detection of nucleotide sequences of interest is described which eliminate the need for use of ancillary nucleotide sequences.

Abstract translation: 提供了不需要体外选择的用于鉴定成功转化的方法。 描述了目的核苷酸序列的直接检测，其消除了使用辅助核苷酸序列的需要。

公开(公告)号：US08148603B2

公开(公告)日：2012-04-03

申请号：US11597661

申请日：2005-05-24

Applicant: Moshe Flaishman ,  Avi Pearl ,  Sara Golobowicz

Inventor： Moshe Flaishman ,  Avi Pearl ,  Sara Golobowicz

IPC: A01H1/00 ,  C07H21/04 ,  C12N15/00

CPC classification number: C12N15/8201 ,  A61K36/60 ,  Y02A50/411 ,  Y02A50/471

Abstract: The present invention relates to transgenic Ficus plants, particularly Ficus carica (fig tree), to a method for producing same, and to Ficus plants, plant materials and plant products produced by or from such genetically modified plant material. More specifically, the present invention relates to transgenic Ficus carica plants and use thereof for producing trees having improved agricultural traits and for the production of foreign proteins and edible vaccines.

Abstract translation: 本发明涉及转基因榕属植物，特别是榕树（无花果树），其生产方法，以及由这种转基因植物材料生产的榕属植物，植物材料和植物产品。 更具体地，本发明涉及转基因榕属植物及其用于生产具有改进的农业性状和用于生产外来蛋白质和食用疫苗的树木的用途。

公开(公告)号：US08097788B2

公开(公告)日：2012-01-17

申请号：US12536355

申请日：2009-08-05

Applicant: Maria Rita Mogno

Inventor： Maria Rita Mogno

IPC: A01H1/00 ,  C07H21/04 ,  C07K14/415 ,  C12N15/00

CPC classification number: A01H5/08 ,  A01G22/00 ,  A01H1/02 ,  C12N15/8201 ,  C12N15/8241 ,  C12N15/8245 ,  C12N15/8247 ,  C12N15/8251 ,  C12N15/8271 ,  C12N15/8274 ,  C12N15/8279 ,  C12N15/8286 ,  C12N15/8289 ,  C12Q1/6895 ,  C12Q2600/13 ,  C12Q2600/156 ,  Y10T436/143333

Abstract: The invention provides seed and plants of tomato hybrid PS01819578 and the parent lines thereof. The invention thus relates to the plants, seeds and tissue cultures of tomato hybrid PS01819578 and the parent lines thereof, and to methods for producing a tomato plant produced by crossing such plants with themselves or with another tomato plant, such as a plant of another genotype. The invention further relates to seeds and plants produced by such crossing. The invention further relates to parts of such plants, including the fruit and gametes of such plants.

Abstract translation: 本发明提供番茄混合PS01819578及其亲本的种子和植物。 因此，本发明涉及番茄杂种PS01819578及其亲本系的植物，种子和组织培养物，以及通过将这些植物与其他番茄植物如另一种基因型的植物杂交而产生的番茄植物的方法 。 本发明还涉及通过这种交叉产生的种子和植物。 本发明还涉及这种植物的部分，包括这种植物的水果和配子。

公开(公告)号：US20110296551A1

公开(公告)日：2011-12-01

申请号：US13131081

申请日：2009-11-25

Applicant: Isabelle Malcuit ,  Alexander Sorokin

Inventor： Isabelle Malcuit ,  Alexander Sorokin

IPC: A01H1/00 ,  C12N1/12 ,  C12N5/04 ,  C12N5/10 ,  C12N15/82 ,  C12N1/00

CPC classification number: C12N15/8201 ,  C12N15/8214 ,  C12N15/8217 ,  C12N15/8257 ,  C12N15/8258 ,  C12N15/8289

Abstract: Method for heterologous RNA species and protein production in plant cell mitochondria comprising introducing into plant cells nucleic acid components that encode heterologous proteins/RNAS under the control of promoters operative in mitochondria, vectors, host cells, plants and uses thereof.

Abstract translation: 用于在植物细胞线粒体中异源RNA种类和蛋白质产生的方法，其包括在植物细胞内引入编码在线粒体，载体，宿主细胞，植物及其用途的启动子控制下的异源蛋白/ RNAS的核酸组分。