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    Polyunsaturated fatty acid production in heterologous organisms using PUFA polyketide synthase systems
    91.
    发明授权
    Polyunsaturated fatty acid production in heterologous organisms using PUFA polyketide synthase systems 有权
    使用PUFA聚酮化合物系统在异源生物体中产生多不饱和脂肪酸

    公开(公告)号：US07759548B2

    公开(公告)日：2010-07-20

    申请号：US11686856

    申请日：2007-03-15

    申请人： James G. Metz ,  Jerry M. Kuner ,  James Casey Lippmeier

    发明人： James G. Metz ,  Jerry M. Kuner ,  James Casey Lippmeier

    IPC分类号： A01H5/00 ,  C12N15/82 ,  C07H21/04 ,  C12N1/20 ,  C12N5/10

    CPC分类号： C12N9/1029 ,  C12N9/93 ,  C12N15/8247 ,  C12P7/6427 ,  C12P7/6472 ,  Y02P20/52

    摘要： Disclosed are novel acyl-CoA synthetases and novel acyltransferases, nucleic acid molecules encoding the same, recombinant nucleic acid molecules and recombinant host cells comprising such nucleic acid molecules, genetically modified organisms (microorganisms and plants) comprising the same, and methods of making and using the same. Also disclosed are genetically modified organisms (e.g., plants, microorganisms) that have been genetically modified to express a PKS-like system for the production of PUFAs (a PUFA PKS system or PUFA synthase), wherein the organisms have been modified to express an acyl-CoA synthetase, to express an acyl transferase, to delete or inactivate a fatty acid synthase (FAS) expressed by the organism, to reduce competition for malonyl CoA with the PUFA synthase or to increase the level of malonyl CoA in the organism, and in one aspect, to inhibit KASII or KASIII. Additional modifications, and methods to make and use such organisms, in addition to PUFAs and oils obtained from such organisms, are disclosed, alone with various products including such PUFAs and oils.

    摘要翻译： 公开了新型酰基辅酶A合成酶和新型酰基转移酶，编码该酰基转移酶的核酸分子，重组核酸分子和包含该核酸分子的重组宿主细胞，包含该核酸分子的转基因生物（微生物和植物），以及制备和使用 一样。 还公开了经遗传修饰以表达用于生产PUFA（PUFA PKS系统或PUFA合酶）的PKS样系统的遗传修饰生物体（例如，植物，微生物），其中生物体被修饰以表达酰基 -CoA合成酶，以表达酰基转移酶，以删除或失活由生物体表达的脂肪酸合酶（FAS），以减少与PUFA合酶的丙二酰辅酶A竞争或增加生物体中丙二酰辅酶A的水平，以及 一方面抑制KASII或KASIII。 除了PUFA和从这些生物获得的油之外，还公开了另外的修饰和制造和使用这些生物体的方法，其中包括各种产品，包括这种PUFA和油。

    METHODS FOR PRODUCING POLYUNSATURATED FATTY ACID AND LIPID CONTAINING POLYUNSATURATED FATTY ACID
    92.
    发明申请
    METHODS FOR PRODUCING POLYUNSATURATED FATTY ACID AND LIPID CONTAINING POLYUNSATURATED FATTY ACID 有权
    生产聚氨酯脂肪酸的方法和含脂肪酸脂肪酸的脂质

    公开(公告)号：US20100167359A1

    公开(公告)日：2010-07-01

    申请号：US12523087

    申请日：2008-01-15

    申请人： Kenji Katano ,  Hiroshi Kawashima

    发明人： Kenji Katano ,  Hiroshi Kawashima

    IPC分类号： C12P7/64 ,  C12N1/14

    CPC分类号： C12P7/6427 ,  A23K20/158 ,  A23K50/40 ,  A23K50/80 ,  A23L33/12 ,  C11B1/10 ,  C11B3/00 ,  C12P7/6472

    摘要： A method for producing a polyunsaturated fatty acid (PUFA) or a lipid containing a PUFA, a microbial cell containing a PUFA, and use of the microbial cell are provided. A method for producing a polyunsaturated fatty acid (PUFA) or a lipid containing a PUFA including culture of a microorganism capable of producing arachidonic acid (ARA) and/or dihomo-gamma-linolenic acid (DGLA) is provided, the method including at least one of the following steps: (a) adding an organic acid in an amount of 0.01 to 5 w/v % to a culture medium after the beginning of main culture; (b) increasing the pH of the culture medium to a range effective for culture after the beginning of the main culture; and (c) adding a metal sulfate in an amount of 0.01 to 0.5 w/w % to the main culture medium.

    摘要翻译： 提供了制备多不饱和脂肪酸（PUFA）或含有PUFA的脂质的方法，含有PUFA的微生物细胞和微生物细胞的使用。 提供了一种生产多不饱和脂肪酸（PUFA）或含有PUFA的脂质的方法，包括能够产生花生四烯酸（ARA）和/或二氢-γ-亚麻酸（DGLA）的微生物的培养物，该方法至少包括 以下步骤之一：（a）在主培养开始后向培养基中加入0.01至5w / v％的有机酸; （b）将培养基的pH值提高到主培养开始后对培养有效的范围; 和（c）向主培养基中加入0.01〜0.5w / w％的金属硫酸盐。

    Desaturase genes, enzymes encoded thereby, and uses thereof
    93.
    发明授权
    Desaturase genes, enzymes encoded thereby, and uses thereof 有权
    去饱和酶基因，由此编码的酶及其用途

    公开(公告)号：US07723503B2

    公开(公告)日：2010-05-25

    申请号：US11621446

    申请日：2007-01-09

    申请人： Pradip Mukerji ,  Suzette L. Pereira ,  Yung-Sheng Huang

    发明人： Pradip Mukerji ,  Suzette L. Pereira ,  Yung-Sheng Huang

    IPC分类号： C07H21/04

    CPC分类号： C12N9/0083 ,  C12P7/6427 ,  C12P7/6472

    摘要： Disclosed are isolated polynucleotides encoding an omega-3 desaturase and a delta-12 desaturase, the enzymes encoded by the isolated polynucleotides, vectors containing the isolated polynucleotides, transgenic hosts that contain the isolated polynucleotides that express the enzymes encoded thereby, methods for producing the desaturase enzymes, and method of using the enzymes to make polyunsaturated fatty acids. The isolated polynucleotides are derived from a fungus, Saprolegnia diclina (ATCC 56851). In particular, omega-3-desaturase may be utilized, for example, in the conversion of arachidonic acid (AA) to eicosapentaenoic acid (EPA). Delta-12 desaturase may be used, for example, in the conversion of oleic acid (OA) to linoleic (LA). EPA or polyunsaturated fatty acids produced therefrom may be added to pharmaceutical compositions, nutritional compositions, animal feeds, as well as other products such as cosmetics.

    摘要翻译： 公开了编码ω-3去饱和酶和δ-12去饱和酶的分离的多核苷酸，由分离的多核苷酸编码的酶，含有分离的多核苷酸的载体，含有表达由其编码的酶的分离的多核苷酸的转基因宿主，用于产生去饱和酶的方法 酶和使用酶制备多不饱和脂肪酸的方法。 分离的多核苷酸衍生自真菌Saprolegnia diclina（ATCC 56851）。 特别地，ω-3-去饱和酶可用于例如花生四烯酸（AA）转化为二十碳五烯酸（EPA）。 Δ-12去饱和酶可以用于例如将油酸（OA）转化为亚油酸（LA）。 由此产生的EPA或多不饱和脂肪酸可以加入到药物组合物，营养组合物，动物饲料中，以及其它产品如化妆品中。

    Recombinant Production Docosahexaenoic Acid (DHA) in Yeast
    94.
    发明申请
    Recombinant Production Docosahexaenoic Acid (DHA) in Yeast 审中-公开
    酵母中重组生产二十二碳六烯酸（DHA）

    公开(公告)号：US20100120103A1

    公开(公告)日：2010-05-13

    申请号：US11721660

    申请日：2005-11-09

    申请人： Villoo Morawala Patell ,  K.R. Rajyashri

    发明人： Villoo Morawala Patell ,  K.R. Rajyashri

    IPC分类号： C12P7/64 ,  C12N1/16 ,  C12N1/19

    CPC分类号： C12P7/6472 ,  C12P7/6427

    摘要： The present invention relates to a specifically novel recombinant method of production of the omega-3 fatty acid, Docosahexaenoic acid by a potentially safe recombinant organism Saccharomyces cerevisiae. The invention describes the process of bioconversion of oleic acid to docosahexaenoic acid through a series of enzymatic conversions facilitated through the cloning of the respective genes into appropriate vectors and the final expression of the DHA in the host cell, Yeast.

    摘要翻译： 本发明涉及通过潜在安全的重组生物酿酒酵母生产ω-3脂肪酸二十二碳六烯酸的特异性新型重组方法。 本发明描述了通过将各基因克隆到适当载体中促进的一系列酶转化和DHA在宿主细胞酵母中的最终表达来将油酸生物转化为二十二碳六烯酸的方法。

    Method for the production of polyunsaturated fatty acids
    95.
    发明授权
    Method for the production of polyunsaturated fatty acids 有权
    生产多不饱和脂肪酸的方法

    公开(公告)号：US07714185B2

    公开(公告)日：2010-05-11

    申请号：US10539891

    申请日：2003-12-11

    申请人： Jonathan A. Napier ,  Olga Sayanova ,  Colin M. Lazarus ,  Baoxiu Qi ,  Ernst Heinz ,  Thorsten Zank ,  Ulrich Zähringer

    发明人： Jonathan A. Napier ,  Olga Sayanova ,  Colin M. Lazarus ,  Baoxiu Qi ,  Ernst Heinz ,  Thorsten Zank ,  Ulrich Zähringer

    IPC分类号： C12N15/82 ,  C12N15/87 ,  C07H21/04

    CPC分类号： C12N9/0083 ,  C12N9/1029 ,  C12N15/8247 ,  C12P7/6427 ,  C12P7/6472

    摘要： The present invention relates to an improved process for the specific production of poly-unsaturated omega-3 and omega-6 fatty acids and a process for the production of triglycerides having an increased content of unsaturated fatty acids, in particular omega-3 and omega-6 fatty acids having at least two double bonds and a 20 or 22 carbon atom chain length. The invention relates to the production of a transgenic organism, preferably a transgenic plant or a transgenic microorganism, hav-ing an increased content of fatty acids, oils or lipids containing C20- or C22-fatty acids with a delta-5, 7, 8, 10 double bond, respectively due to the expression of a delta-8-desaturase and a delta-9-elon-gase from organisms such as plants preferably Algae like Isochrysis galbana or Euglena gracilis. In addition the invention relates to a process for the production of poly unsaturated fatty acids such as Eicosapentaenoic, Arachidonic, Docosapentaenoic or Doosahexaenoic acid through the co-expression of a delta-8-desaturase, a delta-9-elongase and a delta-5 desaturase in organisms such as microorganisms or plants. The invention additionally relates to the use of specific nucleic acid sequences encoding for the aforementioned proteins with delta-8-desaturase-, delta-9-elongase- or delta-5-desaturase-activity, nucleic acid constructs, vectors and organisms containing said nucleic acid sequences. The invention further relates to unsaturated fatty acids and triglycerides having an increased content of at least 1% by weight of unsaturated fatty acids and use thereof.

    摘要翻译： 本发明涉及一种用于特异性生产多不饱和ω-3和ω-6脂肪酸的改进方法和一种具有增加的不饱和脂肪酸含量，特别是ω-3和ω- 具有至少两个双键和20或22个碳原子链长的6种脂肪酸。 本发明涉及转基因生物，优选转基因植物或转基因微生物的生产，其具有增加的含有C20-C22-脂肪酸的脂肪酸，油或脂质含量，其中Δ-5,7,8 ，10双键，分别由于诸如植物优选藻类如等鞭毛藻或小球虫的生物体的δ-8-去饱和酶和δ-9-脱氢酶的表达。 此外，本发明涉及通过共同表达δ-8-去饱和酶，δ-9-延伸酶和δ-5的多不饱和脂肪酸如二十碳五烯酸，花生四烯酸，多糖五烯酸或多沙六酸， 微生物或植物等生物体中的去饱和酶。 本发明还涉及编码上述蛋白质的特定核酸序列与δ-8-去饱和酶，Δ-9-延伸酶或δ-5-去饱和酶活性，核酸构建体，载体和含有所述核酸的生物体的用途 酸序列。 本发明还涉及不饱和脂肪酸和甘油三酸酯，其具有增加的至少1重量％的不饱和脂肪酸的含量及其用途。

    PRODUCTION OF OMEGA-3 FATTY ACIDS IN MICROFLORA OF THRAUSTOCHYTRIALES USING MODIFIED MEDIA
    97.
    发明申请
    PRODUCTION OF OMEGA-3 FATTY ACIDS IN MICROFLORA OF THRAUSTOCHYTRIALES USING MODIFIED MEDIA 审中-公开
    使用修改后的媒体生产谷类微生物中的OMEGA-3脂肪酸

    公开(公告)号：US20100086979A1

    公开(公告)日：2010-04-08

    申请号：US12446776

    申请日：2007-10-09

    申请人： Thomas Kiy ,  Markus Luy ,  Oliver Zeumer

    发明人： Thomas Kiy ,  Markus Luy ,  Oliver Zeumer

    IPC分类号： C12P7/64 ,  C12P1/00

    CPC分类号： C12P7/6472 ,  C12P7/6427

    摘要： The present invention relates to improved methods for the production of omega-3 fatty acids by microorganisms belonging to the microflora of Thraustochytriales using a modified composition of the culture medium. In particular, the invention is directed to a process for the production of omega-3 fatty acids by culturing microflora, such as strains of Ulkenia, Thraustochytrium and/or Schizochytrium, in fermentors which includes the step of culturing the Thraustochytriales microflora in an environment of reduced sodium ions and increased potassium ions.

    摘要翻译： 本发明涉及使用改良的培养基组合物来产生属于破囊壶菌菌群的微生物的ω-3脂肪酸的改进方法。 特别地，本发明涉及通过在发酵罐中培养微生物菌群如乌尔金氏菌属，破囊壶菌属和/或裂殖壶菌属的菌株来生产ω-3脂肪酸的方法，该方法包括在环境中培养破囊壶菌菌群的步骤 减少钠离子和增加钾离子。