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    ANALYSIS OF NUCLEIC ACID AMPLIFICATION CURVES USING WAVELET TRANSFORMATION
    91.
    发明申请
    ANALYSIS OF NUCLEIC ACID AMPLIFICATION CURVES USING WAVELET TRANSFORMATION 有权
    使用小波变换分析核酸放大曲线

    公开(公告)号：US20110039274A1

    公开(公告)日：2011-02-17

    申请号：US12988820

    申请日：2009-04-24

    申请人： Peter D. Ludowise

    发明人： Peter D. Ludowise

    IPC分类号： C12Q1/68 ,  G06F19/00

    CPC分类号： C12Q1/6851 ,  C12Q2561/113 ,  C12Q2537/165

    摘要： A method comprising acquiring amplification data proportional to an amount of nucleic acid present for each of a plurality of PCR cycles includes applying wavelet transformation to the amplification data to determine a PCR cycle corresponding to a point within a growth period of the amplification data, and updating a display A device including a control module, an analysis module and an interface module for initialization of PCR analysis of a nucleic acid sample, receiving amplification data proportional to an amount of nucleic acid present applying wavelet transformation to the amplification data to determine a PCR cycle corresponding to a point within a growth period of the amplification data, and updating a display based on the amplification data is also provided.

    摘要翻译： 一种方法，包括获取与多个PCR循环中的每一个存在的核酸量成比例的扩增数据，包括将小波变换应用于扩增数据，以确定对应于扩增数据生长期内的点的PCR循环，以及更新 显示器包括控制模块，分析模块和用于初始化核酸样品的PCR分析的接口模块的装置，接收与向扩增数据应用小波变换的存在的核酸的量成比例的扩增数据以确定PCR循环 对应于放大数据的生长周期内的点，并且还提供了基于放大数据更新显示。

    Identification and quantification of a plurality of biological (micro)organisms or their components
    92.
    发明授权
    Identification and quantification of a plurality of biological (micro)organisms or their components 有权
    鉴定和定量多个生物（微生物）或其组分

    公开(公告)号：US07875442B2

    公开(公告)日：2011-01-25

    申请号：US11436383

    申请日：2006-05-17

    申请人： Isabelle Alexandre ,  Sylvain Margaine ,  Dieter Husar ,  Nathalie Zammatteo ,  Heinz Koehn ,  José Remacle

    发明人： Isabelle Alexandre ,  Sylvain Margaine ,  Dieter Husar ,  Nathalie Zammatteo ,  Heinz Koehn ,  José Remacle

    IPC分类号： C12P19/34

    CPC分类号： C12Q1/6837 ,  C12Q2561/113

    摘要： Disclosed is a system and method conducting real-time PCR. Unlabeled capture molecules of a specific design are immobilized on a solid support, and contacted with amplicons produced in one or more PCR cycles. Detection of amplicons may take place during or between the PCR cycles while the solid support is in fluidic contact with the PCR solution. In an alternate embodiment detection of the amplicons takes place when the solid support is not in fluidic contact with the PCR solution. The method is suitable for the simultaneous detection and quantification of closely homologous target molecules.

    摘要翻译： 公开了进行实时PCR的系统和方法。 将具体设计的未标记的捕获分子固定在固体支持物上，并与在一个或多个PCR循环中产生的扩增子接触。 扩增子的检测可以在PCR循环期间或之间进行，而固体支持物与PCR溶液流体接触。 在替代实施方案中，当固体支持物不与PCR溶液流体接触时，扩增子的检测发生。 该方法适用于同时检测和定量分析近似同源的靶分子。

    Methods for monitoring polymerase chain reactions
    93.
    发明授权
    Methods for monitoring polymerase chain reactions 有权
    监测聚合酶链反应的方法

    公开(公告)号：US07875425B2

    公开(公告)日：2011-01-25

    申请号：US11270841

    申请日：2005-11-09

    申请人： Stephen J. Gunstream ,  Patrick D. Kinney

    发明人： Stephen J. Gunstream ,  Patrick D. Kinney

    IPC分类号： C12Q1/68 ,  C12P19/34 ,  C12M1/34

    CPC分类号： B01L7/52 ,  B01L2200/147 ,  B01L2200/148 ,  B01L2300/0829 ,  C12Q1/6851 ,  C12Q2561/113 ,  C12Q2527/101

    摘要： A method for calibrating temperature can include cycling temperatures of a set of wells, wherein each well of the set comprises a sample with a spectrally distinguishable species. The method can further include measuring a signal from the spectrally distinguishable species for each well at a temperature during a first temperature cycle, and calibrating the temperatures for measuring the signal from each well during subsequent temperature cycles.

    摘要翻译： 用于校准温度的方法可以包括一组孔的循环温度，其中该组的每个孔包含具有光谱可区分物种的样品。 该方法还可以包括在第一温度循环期间的温度下测量来自每个孔的光谱可区分物质的信号，并且在随后的温度循环期间校准用于测量来自每个孔的信号的温度。

    DETECTION OF PCR PRODUCTS IN GEL ELECTROPHORESIS
    94.
    发明申请
    DETECTION OF PCR PRODUCTS IN GEL ELECTROPHORESIS 失效
    检测凝胶电泳中的PCR产物

    公开(公告)号：US20100330579A1

    公开(公告)日：2010-12-30

    申请号：US12876320

    申请日：2010-09-07

    申请人： Christian Birkner

    发明人： Christian Birkner

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/686 ,  C12Q2565/125 ,  C12Q2563/173 ,  C12Q2561/113

    摘要： Disclosed is a method for analyzing nucleic acids in a sample comprising the following steps: (i) adding a DNA binding dye containing a benzothiazolium or benzoxazolium group to the sample to be analyzed, (ii) carrying out a polymerase chain reaction, (iii) applying the sample to a gel matrix, (iv) separating the nucleic acid molecules according to their size by applying a voltage and (v) excitation with light of a suitable wavelength for the optical visualization of the nucleic acid/DNA binding dye complexes.

    摘要翻译： 本发明公开了一种分析样品中的核酸的方法，包括以下步骤：（i）向待分析的样品中加入含有苯并噻唑鎓或苯并恶唑鎓基团的DNA结合染料，（ii）进行聚合酶链式反应，（iii） 将样品施加到凝胶基质上，（iv）通过施加电压和（v）用适当波长的光激发核酸/ DNA结合染料复合物的光学可视化，根据其大小分离核酸分子。

    Detection and distinguishing infectious bursal disease virus (IBDV) strains by molecular biology method
    95.
    发明授权
    Detection and distinguishing infectious bursal disease virus (IBDV) strains by molecular biology method 有权
    通过分子生物学方法检测和区分传染性法氏囊病病毒（IBDV）菌株

    公开(公告)号：US07858768B2

    公开(公告)日：2010-12-28

    申请号：US12551969

    申请日：2009-09-01

    申请人： Abdul Rahman Omar ,  Mohd Hair-Bejo ,  Ideris Aini ,  Hairul Aini Binti Hamzah

    发明人： Abdul Rahman Omar ,  Mohd Hair-Bejo ,  Ideris Aini ,  Hairul Aini Binti Hamzah

    IPC分类号： C07H21/04 ,  C12Q1/68

    CPC分类号： C12Q1/701 ,  C12Q2561/113

    摘要： The present invention relates to a novel method to detect and differentiate different strains of infectious bursal disease virus (IBDV) in a chicken and other bird sample. RNA was obtained from said samples by using a pair of primer (Primer FVVC & RVVC) in a reverse transcriptase-polymerase chain reaction. Two different primer combinations (Primer IF & IVIR) and (Primer IF & RCLA) and real-time polymerase chain reaction conditions were designed and optimized for rapid differentiation of very virulent and vaccine strains of IBDV based on detection of signatory threshold cycle (Ct) and melting temperature (Tm) values.

    摘要翻译： 本发明涉及一种在鸡和其他鸟类样品中检测和鉴别感染性法氏囊病病毒（IBDV）的不同菌株的新方法。 通过在逆转录酶 - 聚合酶链反应中使用一对引物（引物FVVC和RVVC）从所述样品获得RNA。 设计并优化了两种不同的引物组合（Primer IF和IVIR）和（Primer IF＆RCLA）和实时聚合酶链反应条件，以便基于签名阈值循环（Ct）的检测快速分化IBDV的非常强力和疫苗株， 和熔融温度（Tm）值。