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    METHODS FOR MAKING SACCHARIDE-PROTEIN GLYCOCONJUGATES
    102.
    发明申请
    METHODS FOR MAKING SACCHARIDE-PROTEIN GLYCOCONJUGATES 审中-公开
    制备海草蛋白糖蛋白的方法

    公开(公告)号：US20160297895A1

    公开(公告)日：2016-10-13

    申请号：US15150302

    申请日：2016-05-09

    Applicant: GlaxoSmithKline Biologicals SA

    Inventor： Allan James SAUL ,  Francesca MICOLI

    IPC: C08B37/00

    CPC classification number: C08B37/0006 ,  A61K39/02 ,  A61K39/0275 ,  A61K39/095 ,  A61K39/385 ,  A61K47/6415 ,  A61K47/646 ,  A61K2039/6037 ,  C07K14/34 ,  C07K16/1285 ,  C07K16/44 ,  C07K2317/734 ,  C08B37/0003 ,  C08B37/006 ,  C08B37/0063 ,  C08B37/0081 ,  Y02A50/482

    Abstract: The invention provides a process for the reductive amination of a carbonyl group at the reducing terminus of a polysaccharide, wherein the reductive amination is carried out at a pH between 4 and 5. The invention also provides a process for preparing a conjugate of a polysaccharide and a carrier molecule, comprising the steps of: (a) coupling the polysaccharide to a linker, to form a polysaccharide-linker compound in which the free terminus of the linker is an ester group; and (b) reacting the ester group with a primary amine group in the carrier molecule, to form a polysaccharide-linker-carrier molecule conjugate in which the linker is coupled to the carrier molecule via an amide linkage. The invention also provides a process for reducing contamination of a polysaccharide-linker compound with unreacted linker, comprising a step of precipitating unreacted linker under aqueous conditions at a pH of less than 5. The invention also provides polysaccharide-linker-carrier molecule conjugates and intermediate compounds obtained or obtainable by these processes.

    Abstract translation: 本发明提供了在多糖的还原末端还原胺化羰基的方法，其中还原胺化在pH4至5之间进行。本发明还提供了制备多糖和 载体分子，其包括以下步骤：（a）将多糖偶联至接头，以形成其中接头的游离末端为酯基的多糖 - 接头化合物; 和（b）在载体分子中使酯基与伯胺基反应，以形成多糖 - 连接体 - 载体分子缀合物，其中连接体经由酰胺键与载体分子偶联。 本发明还提供了一种减少多糖 - 接头化合物与未反应接头的污染的方法，包括在pH小于5的水性条件下沉淀未反应的接头的步骤。本发明还提供多糖 - 接头 - 载体分子缀合物和中间体 通过这些方法获得或可获得的化合物。

    RNA PURIFICATION METHODS
    106.
    发明申请
    RNA PURIFICATION METHODS 审中-公开
    RNA纯化方法

    公开(公告)号：US20160024139A1

    公开(公告)日：2016-01-28

    申请号：US14775760

    申请日：2014-03-13

    Applicant: GLAXOSMITHKLINE BIOLOGICALS SA

    Inventor： Francesco BERLANDA SCORZA ,  Yingxia WEN ,  Andrew GEALL ,  Frederick PORTER

    IPC: C07H21/02 ,  B01D15/36 ,  B01D15/38 ,  C07H1/06

    CPC classification number: C07H21/02 ,  B01D15/36 ,  B01D15/38 ,  B01D15/3847 ,  B01D61/14 ,  B01D2015/3838 ,  C07H1/06 ,  C12N15/101 ,  C12N15/1017

    Abstract: Methods for purifying RNA from a sample, comprising one or more steps of tangential flow filtration, hydroxyapatite chromatography, core bead flow-through chromatography, or any combinations thereof. These techniques are useful individually, but show very high efficiency when used in combination, or when performed in particular orders. The methods can purify RNA in a highly efficient manner without unduly compromising potency or stability, to provide compositions in which RNA is substantially cleared of contaminants. Moreover, they can be performed without the need for organic solvents.

    Abstract translation: 用于从样品中纯化RNA的方法，包括一个或多个切向流过滤步骤，羟基磷灰石层析，芯珠流通层析或其任何组合。 这些技术可以单独使用，但是当组合使用时，或者特别是以特定顺序执行时，显示出非常高的效率。 该方法可以高效地纯化RNA，而不会不利地影响效力或稳定性，以提供RNA基本上不含污染物的组合物。 此外，它们可以在不需要有机溶剂的情况下进行。

    PURIFICATION OF STAPHYLOCOCCUS AUREUS TYPE 8 CAPSULAR SACCHARIDES
    107.
    发明申请
    PURIFICATION OF STAPHYLOCOCCUS AUREUS TYPE 8 CAPSULAR SACCHARIDES 有权
    US US US US。。。。。。。。。。。。。。。。。。。。。。

    公开(公告)号：US20150315225A1

    公开(公告)日：2015-11-05

    申请号：US14714097

    申请日：2015-05-15

    Applicant: GLAXOSMITHKLINE BIOLOGICALS SA

    Inventor： Paolo COSTANTINO ,  Maria Rosaria ROMANO ,  Francesco BERTI

    IPC: C07H1/08 ,  C12P19/04 ,  A61K39/085

    CPC classification number: C07H1/08 ,  A61K39/085 ,  A61K2039/55505 ,  A61K2039/6037 ,  C12P19/04

    Abstract: The invention provides a method for releasing capsular polysaccharide from S. aureus type 5 or type 8 cells, comprising the step of treating the cells with acid. The invention further provides a process for purifying capsular polysaccharide from S. aureus type 5 or type 8 cells comprising this method. Other processing steps may be included in the process, such as enzymatic treatment, e.g. to remove nucleic acid, protein and/or peptidoglycan contaminants; diafiltration, e.g. to remove low molecular weight contaminants; anion exchange chromatography, e.g. to remove residual protein; and concentration.

    Abstract translation: 本发明提供了一种从金黄色葡萄球菌5型或8型细胞中释放荚膜多糖的方法，包括用酸处理细胞的步骤。 本发明还提供了一种从包括该方法的金黄色葡萄球菌5型或8型细胞中纯化荚膜多糖的方法。 其他处理步骤可以包括在该方法中，例如酶处理，例如， 去除核酸，蛋白质和/或肽聚糖污染物; 渗滤，例如 去除低分子量污染物; 阴离子交换层析，例如 去除残留蛋白质; 和集中。

    IMMUNOGENIC COMPOSITION
    108.
    发明申请
    IMMUNOGENIC COMPOSITION 审中-公开
    免疫组化

    公开(公告)号：US20140193451A1

    公开(公告)日：2014-07-10

    申请号：US13826932

    申请日：2013-03-14

    Applicant: GLAXOSMITHKLINE BIOLOGICALS SA

    Inventor： VINCENT VERLANT

    IPC: A61K39/09 ,  A61K47/48

    CPC classification number: A61K39/092 ,  A61K47/646

    Abstract: The present invention relates to immunogenic compositions comprising 26 μg-45 μg of pneumolysin and/or PhtD, vaccines comprising the immunogenic compositions and their use in medicine.

    Abstract translation: 本发明涉及包含26μg-45μg气溶素和/或PhtD疫苗的免疫原性组合物，其包含免疫原性组合物及其在药物中的用途。

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